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Mechanisms of Variable p44 Expression by Anaplasma phagocytophilum
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| Zeitschriftentitel: | Infection and Immunity |
|---|---|
| Personen und Körperschaften: | , , , |
| In: | Infection and Immunity, 71, 2003, 10, S. 5650-5661 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Lin, Quan Rikihisa, Yasuko Ohashi, Norio Zhi, Ning Lin, Quan Rikihisa, Yasuko Ohashi, Norio Zhi, Ning |
|---|---|
| author |
Lin, Quan Rikihisa, Yasuko Ohashi, Norio Zhi, Ning |
| spellingShingle |
Lin, Quan Rikihisa, Yasuko Ohashi, Norio Zhi, Ning Infection and Immunity Mechanisms of Variable p44 Expression by Anaplasma phagocytophilum Infectious Diseases Immunology Microbiology Parasitology |
| author_sort |
lin, quan |
| spelling |
Lin, Quan Rikihisa, Yasuko Ohashi, Norio Zhi, Ning 0019-9567 1098-5522 American Society for Microbiology Infectious Diseases Immunology Microbiology Parasitology http://dx.doi.org/10.1128/iai.71.10.5650-5661.2003 <jats:title>ABSTRACT</jats:title> <jats:p> The human intragranulocytic bacterium <jats:italic>Anaplasma phagocytophilum</jats:italic> promotes variation of P44s, which are surface-exposed proteins encoded by a <jats:italic>p44</jats:italic> multigene family. In the present study, the specific <jats:italic>p44</jats:italic> gene expression loci in four strains of <jats:italic>A. phagocytophilum</jats:italic> were identified and it was determined that each consisted of four tandem genes, <jats:italic>tr1</jats:italic> , <jats:italic>omp-1X</jats:italic> , <jats:italic>omp-1N</jats:italic> , and <jats:italic>p44</jats:italic> . A putative σ <jats:sup>70</jats:sup> -type promoter was found upstream of <jats:italic>tr1</jats:italic> . The <jats:italic>p44</jats:italic> genes include a central hypervariable region flanked by conserved regions. The hypervariable region sequence in the <jats:italic>p44</jats:italic> expression locus was duplicated and, regardless of the expression status, conserved at another locus in both low- and high-passage cell cultures of strain NY-37. No significant differences in the hypervariable region were found when we compared <jats:italic>p44</jats:italic> sequences, at the level of cDNA, within the expression locus and within other loci in the genomes of strains NY-37 and HZ. Similarly, in cDNA isolated from patients and from assorted cultures of strains NY-31, NY-36, and NY-37, hypervariable regions of 450 deduced amino acid sequences of various <jats:italic>p44</jats:italic> s within each strain were found to be identical, as were those of <jats:italic>p44</jats:italic> sequences in the genome of strain HZ. These data suggest that variations in <jats:italic>p44</jats:italic> sequences at the level of the <jats:italic>p44</jats:italic> expression locus occur through unidirectional conversion of the entire (nonsegmental) <jats:italic>p44</jats:italic> hypervariable region including flanking regions with a corresponding sequence copied from one of the conserved donor <jats:italic>p44</jats:italic> genomic loci. The data suggest that the P44 antigenic repertoire within the hypervariable region is restricted. </jats:p> Mechanisms of Variable <i>p44</i> Expression by <i>Anaplasma phagocytophilum</i> Infection and Immunity |
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American Society for Microbiology, 2003 |
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Infection and Immunity |
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49 |
| title |
Mechanisms of Variable p44 Expression by Anaplasma phagocytophilum |
| title_unstemmed |
Mechanisms of Variable p44 Expression by Anaplasma phagocytophilum |
| title_full |
Mechanisms of Variable p44 Expression by Anaplasma phagocytophilum |
| title_fullStr |
Mechanisms of Variable p44 Expression by Anaplasma phagocytophilum |
| title_full_unstemmed |
Mechanisms of Variable p44 Expression by Anaplasma phagocytophilum |
| title_short |
Mechanisms of Variable p44 Expression by Anaplasma phagocytophilum |
| title_sort |
mechanisms of variable
<i>p44</i>
expression by
<i>anaplasma phagocytophilum</i> |
| topic |
Infectious Diseases Immunology Microbiology Parasitology |
| url |
http://dx.doi.org/10.1128/iai.71.10.5650-5661.2003 |
| publishDate |
2003 |
| physical |
5650-5661 |
| description |
<jats:title>ABSTRACT</jats:title>
<jats:p>
The human intragranulocytic bacterium
<jats:italic>Anaplasma phagocytophilum</jats:italic>
promotes variation of P44s, which are surface-exposed proteins encoded by a
<jats:italic>p44</jats:italic>
multigene family. In the present study, the specific
<jats:italic>p44</jats:italic>
gene expression loci in four strains of
<jats:italic>A. phagocytophilum</jats:italic>
were identified and it was determined that each consisted of four tandem genes,
<jats:italic>tr1</jats:italic>
,
<jats:italic>omp-1X</jats:italic>
,
<jats:italic>omp-1N</jats:italic>
, and
<jats:italic>p44</jats:italic>
. A putative σ
<jats:sup>70</jats:sup>
-type promoter was found upstream of
<jats:italic>tr1</jats:italic>
. The
<jats:italic>p44</jats:italic>
genes include a central hypervariable region flanked by conserved regions. The hypervariable region sequence in the
<jats:italic>p44</jats:italic>
expression locus was duplicated and, regardless of the expression status, conserved at another locus in both low- and high-passage cell cultures of strain NY-37. No significant differences in the hypervariable region were found when we compared
<jats:italic>p44</jats:italic>
sequences, at the level of cDNA, within the expression locus and within other loci in the genomes of strains NY-37 and HZ. Similarly, in cDNA isolated from patients and from assorted cultures of strains NY-31, NY-36, and NY-37, hypervariable regions of 450 deduced amino acid sequences of various
<jats:italic>p44</jats:italic>
s within each strain were found to be identical, as were those of
<jats:italic>p44</jats:italic>
sequences in the genome of strain HZ. These data suggest that variations in
<jats:italic>p44</jats:italic>
sequences at the level of the
<jats:italic>p44</jats:italic>
expression locus occur through unidirectional conversion of the entire (nonsegmental)
<jats:italic>p44</jats:italic>
hypervariable region including flanking regions with a corresponding sequence copied from one of the conserved donor
<jats:italic>p44</jats:italic>
genomic loci. The data suggest that the P44 antigenic repertoire within the hypervariable region is restricted.
</jats:p> |
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| author | Lin, Quan, Rikihisa, Yasuko, Ohashi, Norio, Zhi, Ning |
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| description | <jats:title>ABSTRACT</jats:title> <jats:p> The human intragranulocytic bacterium <jats:italic>Anaplasma phagocytophilum</jats:italic> promotes variation of P44s, which are surface-exposed proteins encoded by a <jats:italic>p44</jats:italic> multigene family. In the present study, the specific <jats:italic>p44</jats:italic> gene expression loci in four strains of <jats:italic>A. phagocytophilum</jats:italic> were identified and it was determined that each consisted of four tandem genes, <jats:italic>tr1</jats:italic> , <jats:italic>omp-1X</jats:italic> , <jats:italic>omp-1N</jats:italic> , and <jats:italic>p44</jats:italic> . A putative σ <jats:sup>70</jats:sup> -type promoter was found upstream of <jats:italic>tr1</jats:italic> . The <jats:italic>p44</jats:italic> genes include a central hypervariable region flanked by conserved regions. The hypervariable region sequence in the <jats:italic>p44</jats:italic> expression locus was duplicated and, regardless of the expression status, conserved at another locus in both low- and high-passage cell cultures of strain NY-37. No significant differences in the hypervariable region were found when we compared <jats:italic>p44</jats:italic> sequences, at the level of cDNA, within the expression locus and within other loci in the genomes of strains NY-37 and HZ. Similarly, in cDNA isolated from patients and from assorted cultures of strains NY-31, NY-36, and NY-37, hypervariable regions of 450 deduced amino acid sequences of various <jats:italic>p44</jats:italic> s within each strain were found to be identical, as were those of <jats:italic>p44</jats:italic> sequences in the genome of strain HZ. These data suggest that variations in <jats:italic>p44</jats:italic> sequences at the level of the <jats:italic>p44</jats:italic> expression locus occur through unidirectional conversion of the entire (nonsegmental) <jats:italic>p44</jats:italic> hypervariable region including flanking regions with a corresponding sequence copied from one of the conserved donor <jats:italic>p44</jats:italic> genomic loci. The data suggest that the P44 antigenic repertoire within the hypervariable region is restricted. </jats:p> |
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| imprint | American Society for Microbiology, 2003 |
| imprint_str_mv | American Society for Microbiology, 2003 |
| institution | DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, FID-BBI-DE-23, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4 |
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| physical | 5650-5661 |
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| spelling | Lin, Quan Rikihisa, Yasuko Ohashi, Norio Zhi, Ning 0019-9567 1098-5522 American Society for Microbiology Infectious Diseases Immunology Microbiology Parasitology http://dx.doi.org/10.1128/iai.71.10.5650-5661.2003 <jats:title>ABSTRACT</jats:title> <jats:p> The human intragranulocytic bacterium <jats:italic>Anaplasma phagocytophilum</jats:italic> promotes variation of P44s, which are surface-exposed proteins encoded by a <jats:italic>p44</jats:italic> multigene family. In the present study, the specific <jats:italic>p44</jats:italic> gene expression loci in four strains of <jats:italic>A. phagocytophilum</jats:italic> were identified and it was determined that each consisted of four tandem genes, <jats:italic>tr1</jats:italic> , <jats:italic>omp-1X</jats:italic> , <jats:italic>omp-1N</jats:italic> , and <jats:italic>p44</jats:italic> . A putative σ <jats:sup>70</jats:sup> -type promoter was found upstream of <jats:italic>tr1</jats:italic> . The <jats:italic>p44</jats:italic> genes include a central hypervariable region flanked by conserved regions. The hypervariable region sequence in the <jats:italic>p44</jats:italic> expression locus was duplicated and, regardless of the expression status, conserved at another locus in both low- and high-passage cell cultures of strain NY-37. No significant differences in the hypervariable region were found when we compared <jats:italic>p44</jats:italic> sequences, at the level of cDNA, within the expression locus and within other loci in the genomes of strains NY-37 and HZ. Similarly, in cDNA isolated from patients and from assorted cultures of strains NY-31, NY-36, and NY-37, hypervariable regions of 450 deduced amino acid sequences of various <jats:italic>p44</jats:italic> s within each strain were found to be identical, as were those of <jats:italic>p44</jats:italic> sequences in the genome of strain HZ. These data suggest that variations in <jats:italic>p44</jats:italic> sequences at the level of the <jats:italic>p44</jats:italic> expression locus occur through unidirectional conversion of the entire (nonsegmental) <jats:italic>p44</jats:italic> hypervariable region including flanking regions with a corresponding sequence copied from one of the conserved donor <jats:italic>p44</jats:italic> genomic loci. The data suggest that the P44 antigenic repertoire within the hypervariable region is restricted. </jats:p> Mechanisms of Variable <i>p44</i> Expression by <i>Anaplasma phagocytophilum</i> Infection and Immunity |
| spellingShingle | Lin, Quan, Rikihisa, Yasuko, Ohashi, Norio, Zhi, Ning, Infection and Immunity, Mechanisms of Variable p44 Expression by Anaplasma phagocytophilum, Infectious Diseases, Immunology, Microbiology, Parasitology |
| title | Mechanisms of Variable p44 Expression by Anaplasma phagocytophilum |
| title_full | Mechanisms of Variable p44 Expression by Anaplasma phagocytophilum |
| title_fullStr | Mechanisms of Variable p44 Expression by Anaplasma phagocytophilum |
| title_full_unstemmed | Mechanisms of Variable p44 Expression by Anaplasma phagocytophilum |
| title_short | Mechanisms of Variable p44 Expression by Anaplasma phagocytophilum |
| title_sort | mechanisms of variable <i>p44</i> expression by <i>anaplasma phagocytophilum</i> |
| title_unstemmed | Mechanisms of Variable p44 Expression by Anaplasma phagocytophilum |
| topic | Infectious Diseases, Immunology, Microbiology, Parasitology |
| url | http://dx.doi.org/10.1128/iai.71.10.5650-5661.2003 |