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Binding and neutralization of endotoxin by Limulus antilipopolysaccharide factor
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| Zeitschriftentitel: | Infection and Immunity |
|---|---|
| Personen und Körperschaften: | , , , , , , , , , |
| In: | Infection and Immunity, 60, 1992, 6, S. 2506-2513 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Warren, H S Glennon, M L Wainwright, N Amato, S F Black, K M Kirsch, S J Riveau, G R Whyte, R I Zapol, W M Novitsky, T J Warren, H S Glennon, M L Wainwright, N Amato, S F Black, K M Kirsch, S J Riveau, G R Whyte, R I Zapol, W M Novitsky, T J |
|---|---|
| author |
Warren, H S Glennon, M L Wainwright, N Amato, S F Black, K M Kirsch, S J Riveau, G R Whyte, R I Zapol, W M Novitsky, T J |
| spellingShingle |
Warren, H S Glennon, M L Wainwright, N Amato, S F Black, K M Kirsch, S J Riveau, G R Whyte, R I Zapol, W M Novitsky, T J Infection and Immunity Binding and neutralization of endotoxin by Limulus antilipopolysaccharide factor Infectious Diseases Immunology Microbiology Parasitology |
| author_sort |
warren, h s |
| spelling |
Warren, H S Glennon, M L Wainwright, N Amato, S F Black, K M Kirsch, S J Riveau, G R Whyte, R I Zapol, W M Novitsky, T J 0019-9567 1098-5522 American Society for Microbiology Infectious Diseases Immunology Microbiology Parasitology http://dx.doi.org/10.1128/iai.60.6.2506-2513.1992 <jats:p>In order to examine the ability of Limulus antilipopolysaccharide factor (LALF) to bind lipopolysaccharide (LPS), we purified LALF to homogeneity from Limulus amoebocyte lysate and coupled it covalently to agarose beads. LALF-coupled beads captured more tritiated LPS from rough and smooth strains of gram-negative bacteria than did control human serum albumin-coupled beads. Unlabeled homologous and heterologous LPS competed for the binding of 3H-LPS to LALF-coupled beads. LALF bound LPS in a dose-dependent manner as assessed by the precipitation of LPS-LALF complexes with 50% saturated ammonium sulfate. We also studied the ability of LALF to neutralize LPS. LPS preincubated with LALF was less mitogenic for murine splenocytes, was less pyrogenic in the rabbit fever assay, was less lethal in mice which had been sensitized to LPS with actinomycin D, and induced less fever, neutropenia, and pulmonary hypertension when infused into sheep. Our findings extend prior studies which suggested that LALF binds to and neutralizes LPS from multiple strains of gram-negative bacteria.</jats:p> Binding and neutralization of endotoxin by Limulus antilipopolysaccharide factor Infection and Immunity |
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American Society for Microbiology, 1992 |
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| title |
Binding and neutralization of endotoxin by Limulus antilipopolysaccharide factor |
| title_unstemmed |
Binding and neutralization of endotoxin by Limulus antilipopolysaccharide factor |
| title_full |
Binding and neutralization of endotoxin by Limulus antilipopolysaccharide factor |
| title_fullStr |
Binding and neutralization of endotoxin by Limulus antilipopolysaccharide factor |
| title_full_unstemmed |
Binding and neutralization of endotoxin by Limulus antilipopolysaccharide factor |
| title_short |
Binding and neutralization of endotoxin by Limulus antilipopolysaccharide factor |
| title_sort |
binding and neutralization of endotoxin by limulus antilipopolysaccharide factor |
| topic |
Infectious Diseases Immunology Microbiology Parasitology |
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http://dx.doi.org/10.1128/iai.60.6.2506-2513.1992 |
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1992 |
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2506-2513 |
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<jats:p>In order to examine the ability of Limulus antilipopolysaccharide factor (LALF) to bind lipopolysaccharide (LPS), we purified LALF to homogeneity from Limulus amoebocyte lysate and coupled it covalently to agarose beads. LALF-coupled beads captured more tritiated LPS from rough and smooth strains of gram-negative bacteria than did control human serum albumin-coupled beads. Unlabeled homologous and heterologous LPS competed for the binding of 3H-LPS to LALF-coupled beads. LALF bound LPS in a dose-dependent manner as assessed by the precipitation of LPS-LALF complexes with 50% saturated ammonium sulfate. We also studied the ability of LALF to neutralize LPS. LPS preincubated with LALF was less mitogenic for murine splenocytes, was less pyrogenic in the rabbit fever assay, was less lethal in mice which had been sensitized to LPS with actinomycin D, and induced less fever, neutropenia, and pulmonary hypertension when infused into sheep. Our findings extend prior studies which suggested that LALF binds to and neutralizes LPS from multiple strains of gram-negative bacteria.</jats:p> |
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| author | Warren, H S, Glennon, M L, Wainwright, N, Amato, S F, Black, K M, Kirsch, S J, Riveau, G R, Whyte, R I, Zapol, W M, Novitsky, T J |
| author_facet | Warren, H S, Glennon, M L, Wainwright, N, Amato, S F, Black, K M, Kirsch, S J, Riveau, G R, Whyte, R I, Zapol, W M, Novitsky, T J, Warren, H S, Glennon, M L, Wainwright, N, Amato, S F, Black, K M, Kirsch, S J, Riveau, G R, Whyte, R I, Zapol, W M, Novitsky, T J |
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| description | <jats:p>In order to examine the ability of Limulus antilipopolysaccharide factor (LALF) to bind lipopolysaccharide (LPS), we purified LALF to homogeneity from Limulus amoebocyte lysate and coupled it covalently to agarose beads. LALF-coupled beads captured more tritiated LPS from rough and smooth strains of gram-negative bacteria than did control human serum albumin-coupled beads. Unlabeled homologous and heterologous LPS competed for the binding of 3H-LPS to LALF-coupled beads. LALF bound LPS in a dose-dependent manner as assessed by the precipitation of LPS-LALF complexes with 50% saturated ammonium sulfate. We also studied the ability of LALF to neutralize LPS. LPS preincubated with LALF was less mitogenic for murine splenocytes, was less pyrogenic in the rabbit fever assay, was less lethal in mice which had been sensitized to LPS with actinomycin D, and induced less fever, neutropenia, and pulmonary hypertension when infused into sheep. Our findings extend prior studies which suggested that LALF binds to and neutralizes LPS from multiple strains of gram-negative bacteria.</jats:p> |
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| imprint | American Society for Microbiology, 1992 |
| imprint_str_mv | American Society for Microbiology, 1992 |
| institution | DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, FID-BBI-DE-23, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161 |
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| physical | 2506-2513 |
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| spelling | Warren, H S Glennon, M L Wainwright, N Amato, S F Black, K M Kirsch, S J Riveau, G R Whyte, R I Zapol, W M Novitsky, T J 0019-9567 1098-5522 American Society for Microbiology Infectious Diseases Immunology Microbiology Parasitology http://dx.doi.org/10.1128/iai.60.6.2506-2513.1992 <jats:p>In order to examine the ability of Limulus antilipopolysaccharide factor (LALF) to bind lipopolysaccharide (LPS), we purified LALF to homogeneity from Limulus amoebocyte lysate and coupled it covalently to agarose beads. LALF-coupled beads captured more tritiated LPS from rough and smooth strains of gram-negative bacteria than did control human serum albumin-coupled beads. Unlabeled homologous and heterologous LPS competed for the binding of 3H-LPS to LALF-coupled beads. LALF bound LPS in a dose-dependent manner as assessed by the precipitation of LPS-LALF complexes with 50% saturated ammonium sulfate. We also studied the ability of LALF to neutralize LPS. LPS preincubated with LALF was less mitogenic for murine splenocytes, was less pyrogenic in the rabbit fever assay, was less lethal in mice which had been sensitized to LPS with actinomycin D, and induced less fever, neutropenia, and pulmonary hypertension when infused into sheep. Our findings extend prior studies which suggested that LALF binds to and neutralizes LPS from multiple strains of gram-negative bacteria.</jats:p> Binding and neutralization of endotoxin by Limulus antilipopolysaccharide factor Infection and Immunity |
| spellingShingle | Warren, H S, Glennon, M L, Wainwright, N, Amato, S F, Black, K M, Kirsch, S J, Riveau, G R, Whyte, R I, Zapol, W M, Novitsky, T J, Infection and Immunity, Binding and neutralization of endotoxin by Limulus antilipopolysaccharide factor, Infectious Diseases, Immunology, Microbiology, Parasitology |
| title | Binding and neutralization of endotoxin by Limulus antilipopolysaccharide factor |
| title_full | Binding and neutralization of endotoxin by Limulus antilipopolysaccharide factor |
| title_fullStr | Binding and neutralization of endotoxin by Limulus antilipopolysaccharide factor |
| title_full_unstemmed | Binding and neutralization of endotoxin by Limulus antilipopolysaccharide factor |
| title_short | Binding and neutralization of endotoxin by Limulus antilipopolysaccharide factor |
| title_sort | binding and neutralization of endotoxin by limulus antilipopolysaccharide factor |
| title_unstemmed | Binding and neutralization of endotoxin by Limulus antilipopolysaccharide factor |
| topic | Infectious Diseases, Immunology, Microbiology, Parasitology |
| url | http://dx.doi.org/10.1128/iai.60.6.2506-2513.1992 |