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The Iron-Sensing Fur Regulator Controls Expression Timing and Levels of Salmonella Pathogenicity Island 2 Genes in the Course of Environmental Acidification
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| Zeitschriftentitel: | Infection and Immunity |
|---|---|
| Personen und Körperschaften: | , , , , , |
| In: | Infection and Immunity, 82, 2014, 6, S. 2203-2210 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
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| Schlagwörter: |
| author_facet |
Choi, Eunna Kim, Hyunkeun Lee, Hwiseop Nam, Daesil Choi, Jeongjoon Shin, Dongwoo Choi, Eunna Kim, Hyunkeun Lee, Hwiseop Nam, Daesil Choi, Jeongjoon Shin, Dongwoo |
|---|---|
| author |
Choi, Eunna Kim, Hyunkeun Lee, Hwiseop Nam, Daesil Choi, Jeongjoon Shin, Dongwoo |
| spellingShingle |
Choi, Eunna Kim, Hyunkeun Lee, Hwiseop Nam, Daesil Choi, Jeongjoon Shin, Dongwoo Infection and Immunity The Iron-Sensing Fur Regulator Controls Expression Timing and Levels of Salmonella Pathogenicity Island 2 Genes in the Course of Environmental Acidification Infectious Diseases Immunology Microbiology Parasitology |
| author_sort |
choi, eunna |
| spelling |
Choi, Eunna Kim, Hyunkeun Lee, Hwiseop Nam, Daesil Choi, Jeongjoon Shin, Dongwoo 0019-9567 1098-5522 American Society for Microbiology Infectious Diseases Immunology Microbiology Parasitology http://dx.doi.org/10.1128/iai.01625-13 <jats:title>ABSTRACT</jats:title> <jats:p> In order to survive inside macrophages, <jats:named-content content-type="genus-species">Salmonella</jats:named-content> produces a series of proteins encoded by genes within <jats:named-content content-type="genus-species">Salmonella</jats:named-content> pathogenicity island 2 (SPI-2). In the present study, we report that Fur, a central regulator of iron utilization, negatively controls the expression of SPI-2 genes. Time course analysis of SPI-2 expression after the entry of <jats:named-content content-type="genus-species">Salmonella</jats:named-content> into macrophages revealed that SPI-2 genes are induced earlier and at higher levels in the absence of the Fur regulator. It was hypothesized that Fur repressed the SPI-2 expression that was activated during acidification of the phagosome. Indeed, as pH was lowered from pH 7.0 to pH 5.5, the lack of Fur enabled SPI-2 gene expression to be induced at higher pH and to be expressed at higher levels. Fur controlled SPI-2 genes via repression of the SsrB response regulator, a primary activator of SPI-2 expression. Fur repressed <jats:italic>ssrB</jats:italic> expression both inside macrophages and under acidic conditions, which we ascribe to the direct binding of Fur to the <jats:italic>ssrB</jats:italic> promoter. Our study suggests that <jats:named-content content-type="genus-species">Salmonella</jats:named-content> could employ iron inside the phagosome to precisely control the timing and levels of SPI-2 expression inside macrophages. </jats:p> The Iron-Sensing Fur Regulator Controls Expression Timing and Levels of Salmonella Pathogenicity Island 2 Genes in the Course of Environmental Acidification Infection and Immunity |
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10.1128/iai.01625-13 |
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American Society for Microbiology, 2014 |
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| title |
The Iron-Sensing Fur Regulator Controls Expression Timing and Levels of Salmonella Pathogenicity Island 2 Genes in the Course of Environmental Acidification |
| title_unstemmed |
The Iron-Sensing Fur Regulator Controls Expression Timing and Levels of Salmonella Pathogenicity Island 2 Genes in the Course of Environmental Acidification |
| title_full |
The Iron-Sensing Fur Regulator Controls Expression Timing and Levels of Salmonella Pathogenicity Island 2 Genes in the Course of Environmental Acidification |
| title_fullStr |
The Iron-Sensing Fur Regulator Controls Expression Timing and Levels of Salmonella Pathogenicity Island 2 Genes in the Course of Environmental Acidification |
| title_full_unstemmed |
The Iron-Sensing Fur Regulator Controls Expression Timing and Levels of Salmonella Pathogenicity Island 2 Genes in the Course of Environmental Acidification |
| title_short |
The Iron-Sensing Fur Regulator Controls Expression Timing and Levels of Salmonella Pathogenicity Island 2 Genes in the Course of Environmental Acidification |
| title_sort |
the iron-sensing fur regulator controls expression timing and levels of salmonella pathogenicity island 2 genes in the course of environmental acidification |
| topic |
Infectious Diseases Immunology Microbiology Parasitology |
| url |
http://dx.doi.org/10.1128/iai.01625-13 |
| publishDate |
2014 |
| physical |
2203-2210 |
| description |
<jats:title>ABSTRACT</jats:title>
<jats:p>
In order to survive inside macrophages,
<jats:named-content content-type="genus-species">Salmonella</jats:named-content>
produces a series of proteins encoded by genes within
<jats:named-content content-type="genus-species">Salmonella</jats:named-content>
pathogenicity island 2 (SPI-2). In the present study, we report that Fur, a central regulator of iron utilization, negatively controls the expression of SPI-2 genes. Time course analysis of SPI-2 expression after the entry of
<jats:named-content content-type="genus-species">Salmonella</jats:named-content>
into macrophages revealed that SPI-2 genes are induced earlier and at higher levels in the absence of the Fur regulator. It was hypothesized that Fur repressed the SPI-2 expression that was activated during acidification of the phagosome. Indeed, as pH was lowered from pH 7.0 to pH 5.5, the lack of Fur enabled SPI-2 gene expression to be induced at higher pH and to be expressed at higher levels. Fur controlled SPI-2 genes via repression of the SsrB response regulator, a primary activator of SPI-2 expression. Fur repressed
<jats:italic>ssrB</jats:italic>
expression both inside macrophages and under acidic conditions, which we ascribe to the direct binding of Fur to the
<jats:italic>ssrB</jats:italic>
promoter. Our study suggests that
<jats:named-content content-type="genus-species">Salmonella</jats:named-content>
could employ iron inside the phagosome to precisely control the timing and levels of SPI-2 expression inside macrophages.
</jats:p> |
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| author | Choi, Eunna, Kim, Hyunkeun, Lee, Hwiseop, Nam, Daesil, Choi, Jeongjoon, Shin, Dongwoo |
| author_facet | Choi, Eunna, Kim, Hyunkeun, Lee, Hwiseop, Nam, Daesil, Choi, Jeongjoon, Shin, Dongwoo, Choi, Eunna, Kim, Hyunkeun, Lee, Hwiseop, Nam, Daesil, Choi, Jeongjoon, Shin, Dongwoo |
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| description | <jats:title>ABSTRACT</jats:title> <jats:p> In order to survive inside macrophages, <jats:named-content content-type="genus-species">Salmonella</jats:named-content> produces a series of proteins encoded by genes within <jats:named-content content-type="genus-species">Salmonella</jats:named-content> pathogenicity island 2 (SPI-2). In the present study, we report that Fur, a central regulator of iron utilization, negatively controls the expression of SPI-2 genes. Time course analysis of SPI-2 expression after the entry of <jats:named-content content-type="genus-species">Salmonella</jats:named-content> into macrophages revealed that SPI-2 genes are induced earlier and at higher levels in the absence of the Fur regulator. It was hypothesized that Fur repressed the SPI-2 expression that was activated during acidification of the phagosome. Indeed, as pH was lowered from pH 7.0 to pH 5.5, the lack of Fur enabled SPI-2 gene expression to be induced at higher pH and to be expressed at higher levels. Fur controlled SPI-2 genes via repression of the SsrB response regulator, a primary activator of SPI-2 expression. Fur repressed <jats:italic>ssrB</jats:italic> expression both inside macrophages and under acidic conditions, which we ascribe to the direct binding of Fur to the <jats:italic>ssrB</jats:italic> promoter. Our study suggests that <jats:named-content content-type="genus-species">Salmonella</jats:named-content> could employ iron inside the phagosome to precisely control the timing and levels of SPI-2 expression inside macrophages. </jats:p> |
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| spelling | Choi, Eunna Kim, Hyunkeun Lee, Hwiseop Nam, Daesil Choi, Jeongjoon Shin, Dongwoo 0019-9567 1098-5522 American Society for Microbiology Infectious Diseases Immunology Microbiology Parasitology http://dx.doi.org/10.1128/iai.01625-13 <jats:title>ABSTRACT</jats:title> <jats:p> In order to survive inside macrophages, <jats:named-content content-type="genus-species">Salmonella</jats:named-content> produces a series of proteins encoded by genes within <jats:named-content content-type="genus-species">Salmonella</jats:named-content> pathogenicity island 2 (SPI-2). In the present study, we report that Fur, a central regulator of iron utilization, negatively controls the expression of SPI-2 genes. Time course analysis of SPI-2 expression after the entry of <jats:named-content content-type="genus-species">Salmonella</jats:named-content> into macrophages revealed that SPI-2 genes are induced earlier and at higher levels in the absence of the Fur regulator. It was hypothesized that Fur repressed the SPI-2 expression that was activated during acidification of the phagosome. Indeed, as pH was lowered from pH 7.0 to pH 5.5, the lack of Fur enabled SPI-2 gene expression to be induced at higher pH and to be expressed at higher levels. Fur controlled SPI-2 genes via repression of the SsrB response regulator, a primary activator of SPI-2 expression. Fur repressed <jats:italic>ssrB</jats:italic> expression both inside macrophages and under acidic conditions, which we ascribe to the direct binding of Fur to the <jats:italic>ssrB</jats:italic> promoter. Our study suggests that <jats:named-content content-type="genus-species">Salmonella</jats:named-content> could employ iron inside the phagosome to precisely control the timing and levels of SPI-2 expression inside macrophages. </jats:p> The Iron-Sensing Fur Regulator Controls Expression Timing and Levels of Salmonella Pathogenicity Island 2 Genes in the Course of Environmental Acidification Infection and Immunity |
| spellingShingle | Choi, Eunna, Kim, Hyunkeun, Lee, Hwiseop, Nam, Daesil, Choi, Jeongjoon, Shin, Dongwoo, Infection and Immunity, The Iron-Sensing Fur Regulator Controls Expression Timing and Levels of Salmonella Pathogenicity Island 2 Genes in the Course of Environmental Acidification, Infectious Diseases, Immunology, Microbiology, Parasitology |
| title | The Iron-Sensing Fur Regulator Controls Expression Timing and Levels of Salmonella Pathogenicity Island 2 Genes in the Course of Environmental Acidification |
| title_full | The Iron-Sensing Fur Regulator Controls Expression Timing and Levels of Salmonella Pathogenicity Island 2 Genes in the Course of Environmental Acidification |
| title_fullStr | The Iron-Sensing Fur Regulator Controls Expression Timing and Levels of Salmonella Pathogenicity Island 2 Genes in the Course of Environmental Acidification |
| title_full_unstemmed | The Iron-Sensing Fur Regulator Controls Expression Timing and Levels of Salmonella Pathogenicity Island 2 Genes in the Course of Environmental Acidification |
| title_short | The Iron-Sensing Fur Regulator Controls Expression Timing and Levels of Salmonella Pathogenicity Island 2 Genes in the Course of Environmental Acidification |
| title_sort | the iron-sensing fur regulator controls expression timing and levels of salmonella pathogenicity island 2 genes in the course of environmental acidification |
| title_unstemmed | The Iron-Sensing Fur Regulator Controls Expression Timing and Levels of Salmonella Pathogenicity Island 2 Genes in the Course of Environmental Acidification |
| topic | Infectious Diseases, Immunology, Microbiology, Parasitology |
| url | http://dx.doi.org/10.1128/iai.01625-13 |