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In Vitro Characterization of the Microglial Inflammatory Response to Streptococcus suis , an Important Emerging Zoonotic Agent of Meningitis
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| Zeitschriftentitel: | Infection and Immunity |
|---|---|
| Personen und Körperschaften: | , , , , , , , |
| In: | Infection and Immunity, 78, 2010, 12, S. 5074-5085 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
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| Schlagwörter: |
| author_facet |
Domínguez-Punaro, María de la Cruz Segura, Mariela Contreras, Irazú Lachance, Claude Houde, Mathieu Lecours, Marie-Pier Olivier, Martin Gottschalk, Marcelo Domínguez-Punaro, María de la Cruz Segura, Mariela Contreras, Irazú Lachance, Claude Houde, Mathieu Lecours, Marie-Pier Olivier, Martin Gottschalk, Marcelo |
|---|---|
| author |
Domínguez-Punaro, María de la Cruz Segura, Mariela Contreras, Irazú Lachance, Claude Houde, Mathieu Lecours, Marie-Pier Olivier, Martin Gottschalk, Marcelo |
| spellingShingle |
Domínguez-Punaro, María de la Cruz Segura, Mariela Contreras, Irazú Lachance, Claude Houde, Mathieu Lecours, Marie-Pier Olivier, Martin Gottschalk, Marcelo Infection and Immunity In Vitro Characterization of the Microglial Inflammatory Response to Streptococcus suis , an Important Emerging Zoonotic Agent of Meningitis Infectious Diseases Immunology Microbiology Parasitology |
| author_sort |
domínguez-punaro, maría de la cruz |
| spelling |
Domínguez-Punaro, María de la Cruz Segura, Mariela Contreras, Irazú Lachance, Claude Houde, Mathieu Lecours, Marie-Pier Olivier, Martin Gottschalk, Marcelo 0019-9567 1098-5522 American Society for Microbiology Infectious Diseases Immunology Microbiology Parasitology http://dx.doi.org/10.1128/iai.00698-10 <jats:title>ABSTRACT</jats:title> <jats:p> <jats:italic>Streptococcus suis</jats:italic> is an important swine and human pathogen responsible for septicemia and meningitis. <jats:italic>In vivo</jats:italic> research in mice suggested that in the brain, microglia might be involved in activating the inflammatory response against <jats:italic>S. suis</jats:italic> . The aim of this study was to better understand the interactions between <jats:italic>S. suis</jats:italic> and microglia. Murine microglial cells were infected with a virulent wild-type strain of <jats:italic>S. suis</jats:italic> . Two isogenic mutants deficient at either capsular polysaccharide (CPS) or hemolysin production were also included. CPS contributed to <jats:italic>S. suis</jats:italic> resistance to phagocytosis and regulated the inflammatory response by hiding proinflammatory components from the bacterial cell wall, while the absence of hemolysin, a potential cytotoxic factor, did not have a major impact on <jats:italic>S. suis</jats:italic> interactions with microglia. Wild-type <jats:italic>S. suis</jats:italic> induced enhanced expression of Toll-like receptor 2 by microglial cells, as well as phophotyrosine, protein kinase C, and different mitogen-activated protein kinase signaling events. However, cells infected with the CPS-deficient mutant showed overall stronger and more sustained phosphorylation profiles. CPS also modulated inducible nitric oxide synthase expression and further nitric oxide production from <jats:italic>S. suis</jats:italic> -infected microglia. Finally, <jats:italic>S. suis</jats:italic> -induced NF-κB translocation was faster for cells stimulated with the CPS-deficient mutant, suggesting that bacterial cell wall components are potent inducers of NF-κB. These results contribute to increase the knowledge of mechanisms underlying <jats:italic>S. suis</jats:italic> inflammation in the brain and will be useful in designing more efficient anti-inflammatory strategies for meningitis. </jats:p> <i>In Vitro</i> Characterization of the Microglial Inflammatory Response to <i>Streptococcus suis</i> , an Important Emerging Zoonotic Agent of Meningitis Infection and Immunity |
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| title |
In Vitro Characterization of the Microglial Inflammatory Response to Streptococcus suis , an Important Emerging Zoonotic Agent of Meningitis |
| title_unstemmed |
In Vitro Characterization of the Microglial Inflammatory Response to Streptococcus suis , an Important Emerging Zoonotic Agent of Meningitis |
| title_full |
In Vitro Characterization of the Microglial Inflammatory Response to Streptococcus suis , an Important Emerging Zoonotic Agent of Meningitis |
| title_fullStr |
In Vitro Characterization of the Microglial Inflammatory Response to Streptococcus suis , an Important Emerging Zoonotic Agent of Meningitis |
| title_full_unstemmed |
In Vitro Characterization of the Microglial Inflammatory Response to Streptococcus suis , an Important Emerging Zoonotic Agent of Meningitis |
| title_short |
In Vitro Characterization of the Microglial Inflammatory Response to Streptococcus suis , an Important Emerging Zoonotic Agent of Meningitis |
| title_sort |
<i>in vitro</i>
characterization of the microglial inflammatory response to
<i>streptococcus suis</i>
, an important emerging zoonotic agent of meningitis |
| topic |
Infectious Diseases Immunology Microbiology Parasitology |
| url |
http://dx.doi.org/10.1128/iai.00698-10 |
| publishDate |
2010 |
| physical |
5074-5085 |
| description |
<jats:title>ABSTRACT</jats:title>
<jats:p>
<jats:italic>Streptococcus suis</jats:italic>
is an important swine and human pathogen responsible for septicemia and meningitis.
<jats:italic>In vivo</jats:italic>
research in mice suggested that in the brain, microglia might be involved in activating the inflammatory response against
<jats:italic>S. suis</jats:italic>
. The aim of this study was to better understand the interactions between
<jats:italic>S. suis</jats:italic>
and microglia. Murine microglial cells were infected with a virulent wild-type strain of
<jats:italic>S. suis</jats:italic>
. Two isogenic mutants deficient at either capsular polysaccharide (CPS) or hemolysin production were also included. CPS contributed to
<jats:italic>S. suis</jats:italic>
resistance to phagocytosis and regulated the inflammatory response by hiding proinflammatory components from the bacterial cell wall, while the absence of hemolysin, a potential cytotoxic factor, did not have a major impact on
<jats:italic>S. suis</jats:italic>
interactions with microglia. Wild-type
<jats:italic>S. suis</jats:italic>
induced enhanced expression of Toll-like receptor 2 by microglial cells, as well as phophotyrosine, protein kinase C, and different mitogen-activated protein kinase signaling events. However, cells infected with the CPS-deficient mutant showed overall stronger and more sustained phosphorylation profiles. CPS also modulated inducible nitric oxide synthase expression and further nitric oxide production from
<jats:italic>S. suis</jats:italic>
-infected microglia. Finally,
<jats:italic>S. suis</jats:italic>
-induced NF-κB translocation was faster for cells stimulated with the CPS-deficient mutant, suggesting that bacterial cell wall components are potent inducers of NF-κB. These results contribute to increase the knowledge of mechanisms underlying
<jats:italic>S. suis</jats:italic>
inflammation in the brain and will be useful in designing more efficient anti-inflammatory strategies for meningitis.
</jats:p> |
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| author | Domínguez-Punaro, María de la Cruz, Segura, Mariela, Contreras, Irazú, Lachance, Claude, Houde, Mathieu, Lecours, Marie-Pier, Olivier, Martin, Gottschalk, Marcelo |
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| description | <jats:title>ABSTRACT</jats:title> <jats:p> <jats:italic>Streptococcus suis</jats:italic> is an important swine and human pathogen responsible for septicemia and meningitis. <jats:italic>In vivo</jats:italic> research in mice suggested that in the brain, microglia might be involved in activating the inflammatory response against <jats:italic>S. suis</jats:italic> . The aim of this study was to better understand the interactions between <jats:italic>S. suis</jats:italic> and microglia. Murine microglial cells were infected with a virulent wild-type strain of <jats:italic>S. suis</jats:italic> . Two isogenic mutants deficient at either capsular polysaccharide (CPS) or hemolysin production were also included. CPS contributed to <jats:italic>S. suis</jats:italic> resistance to phagocytosis and regulated the inflammatory response by hiding proinflammatory components from the bacterial cell wall, while the absence of hemolysin, a potential cytotoxic factor, did not have a major impact on <jats:italic>S. suis</jats:italic> interactions with microglia. Wild-type <jats:italic>S. suis</jats:italic> induced enhanced expression of Toll-like receptor 2 by microglial cells, as well as phophotyrosine, protein kinase C, and different mitogen-activated protein kinase signaling events. However, cells infected with the CPS-deficient mutant showed overall stronger and more sustained phosphorylation profiles. CPS also modulated inducible nitric oxide synthase expression and further nitric oxide production from <jats:italic>S. suis</jats:italic> -infected microglia. Finally, <jats:italic>S. suis</jats:italic> -induced NF-κB translocation was faster for cells stimulated with the CPS-deficient mutant, suggesting that bacterial cell wall components are potent inducers of NF-κB. These results contribute to increase the knowledge of mechanisms underlying <jats:italic>S. suis</jats:italic> inflammation in the brain and will be useful in designing more efficient anti-inflammatory strategies for meningitis. </jats:p> |
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| spelling | Domínguez-Punaro, María de la Cruz Segura, Mariela Contreras, Irazú Lachance, Claude Houde, Mathieu Lecours, Marie-Pier Olivier, Martin Gottschalk, Marcelo 0019-9567 1098-5522 American Society for Microbiology Infectious Diseases Immunology Microbiology Parasitology http://dx.doi.org/10.1128/iai.00698-10 <jats:title>ABSTRACT</jats:title> <jats:p> <jats:italic>Streptococcus suis</jats:italic> is an important swine and human pathogen responsible for septicemia and meningitis. <jats:italic>In vivo</jats:italic> research in mice suggested that in the brain, microglia might be involved in activating the inflammatory response against <jats:italic>S. suis</jats:italic> . The aim of this study was to better understand the interactions between <jats:italic>S. suis</jats:italic> and microglia. Murine microglial cells were infected with a virulent wild-type strain of <jats:italic>S. suis</jats:italic> . Two isogenic mutants deficient at either capsular polysaccharide (CPS) or hemolysin production were also included. CPS contributed to <jats:italic>S. suis</jats:italic> resistance to phagocytosis and regulated the inflammatory response by hiding proinflammatory components from the bacterial cell wall, while the absence of hemolysin, a potential cytotoxic factor, did not have a major impact on <jats:italic>S. suis</jats:italic> interactions with microglia. Wild-type <jats:italic>S. suis</jats:italic> induced enhanced expression of Toll-like receptor 2 by microglial cells, as well as phophotyrosine, protein kinase C, and different mitogen-activated protein kinase signaling events. However, cells infected with the CPS-deficient mutant showed overall stronger and more sustained phosphorylation profiles. CPS also modulated inducible nitric oxide synthase expression and further nitric oxide production from <jats:italic>S. suis</jats:italic> -infected microglia. Finally, <jats:italic>S. suis</jats:italic> -induced NF-κB translocation was faster for cells stimulated with the CPS-deficient mutant, suggesting that bacterial cell wall components are potent inducers of NF-κB. These results contribute to increase the knowledge of mechanisms underlying <jats:italic>S. suis</jats:italic> inflammation in the brain and will be useful in designing more efficient anti-inflammatory strategies for meningitis. </jats:p> <i>In Vitro</i> Characterization of the Microglial Inflammatory Response to <i>Streptococcus suis</i> , an Important Emerging Zoonotic Agent of Meningitis Infection and Immunity |
| spellingShingle | Domínguez-Punaro, María de la Cruz, Segura, Mariela, Contreras, Irazú, Lachance, Claude, Houde, Mathieu, Lecours, Marie-Pier, Olivier, Martin, Gottschalk, Marcelo, Infection and Immunity, In Vitro Characterization of the Microglial Inflammatory Response to Streptococcus suis , an Important Emerging Zoonotic Agent of Meningitis, Infectious Diseases, Immunology, Microbiology, Parasitology |
| title | In Vitro Characterization of the Microglial Inflammatory Response to Streptococcus suis , an Important Emerging Zoonotic Agent of Meningitis |
| title_full | In Vitro Characterization of the Microglial Inflammatory Response to Streptococcus suis , an Important Emerging Zoonotic Agent of Meningitis |
| title_fullStr | In Vitro Characterization of the Microglial Inflammatory Response to Streptococcus suis , an Important Emerging Zoonotic Agent of Meningitis |
| title_full_unstemmed | In Vitro Characterization of the Microglial Inflammatory Response to Streptococcus suis , an Important Emerging Zoonotic Agent of Meningitis |
| title_short | In Vitro Characterization of the Microglial Inflammatory Response to Streptococcus suis , an Important Emerging Zoonotic Agent of Meningitis |
| title_sort | <i>in vitro</i> characterization of the microglial inflammatory response to <i>streptococcus suis</i> , an important emerging zoonotic agent of meningitis |
| title_unstemmed | In Vitro Characterization of the Microglial Inflammatory Response to Streptococcus suis , an Important Emerging Zoonotic Agent of Meningitis |
| topic | Infectious Diseases, Immunology, Microbiology, Parasitology |
| url | http://dx.doi.org/10.1128/iai.00698-10 |