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Measurement of T-Lymphocyte Responses in Whole-Blood Cultures Using Newly Synthesized DNA and ATP
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| Zeitschriftentitel: | Clinical Diagnostic Laboratory Immunology |
|---|---|
| Personen und Körperschaften: | , , , |
| In: | Clinical Diagnostic Laboratory Immunology, 7, 2000, 2, S. 307-311 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Sottong, P. R. Rosebrock, J. A. Britz, J. A. Kramer, T. R. Sottong, P. R. Rosebrock, J. A. Britz, J. A. Kramer, T. R. |
|---|---|
| author |
Sottong, P. R. Rosebrock, J. A. Britz, J. A. Kramer, T. R. |
| spellingShingle |
Sottong, P. R. Rosebrock, J. A. Britz, J. A. Kramer, T. R. Clinical Diagnostic Laboratory Immunology Measurement of T-Lymphocyte Responses in Whole-Blood Cultures Using Newly Synthesized DNA and ATP Microbiology (medical) Clinical Biochemistry Immunology Immunology and Allergy |
| author_sort |
sottong, p. r. |
| spelling |
Sottong, P. R. Rosebrock, J. A. Britz, J. A. Kramer, T. R. 1071-412X 1098-6588 American Society for Microbiology Microbiology (medical) Clinical Biochemistry Immunology Immunology and Allergy http://dx.doi.org/10.1128/cdli.7.2.307-311.2000 <jats:title>ABSTRACT</jats:title> <jats:p> The proliferative response is most frequently determined by estimating the amount of [ <jats:sup>3</jats:sup> H]thymidine incorporated into newly synthesized DNA. The [ <jats:sup>3</jats:sup> H]thymidine procedure requires the use of radioisotopes as well as lengthy periods of incubation (>72 h). An alternative method of assessing T-lymphocyte activation in whole-blood cultures involves the measurement of the nucleotide ATP instead of [ <jats:sup>3</jats:sup> H]thymidine incorporation. In addition, the Luminetics assay of T-cell activation measures specific T-lymphocyte subset responses through the use of paramagnetic particles coated with monoclonal antibodies against CD antigens. This assay permits rapid (24 h) analysis of lymphocyte subset activation responses to mitogens and recall antigens in small amounts of blood. </jats:p> Measurement of T-Lymphocyte Responses in Whole-Blood Cultures Using Newly Synthesized DNA and ATP Clinical Diagnostic Laboratory Immunology |
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| title |
Measurement of T-Lymphocyte Responses in Whole-Blood Cultures Using Newly Synthesized DNA and ATP |
| title_unstemmed |
Measurement of T-Lymphocyte Responses in Whole-Blood Cultures Using Newly Synthesized DNA and ATP |
| title_full |
Measurement of T-Lymphocyte Responses in Whole-Blood Cultures Using Newly Synthesized DNA and ATP |
| title_fullStr |
Measurement of T-Lymphocyte Responses in Whole-Blood Cultures Using Newly Synthesized DNA and ATP |
| title_full_unstemmed |
Measurement of T-Lymphocyte Responses in Whole-Blood Cultures Using Newly Synthesized DNA and ATP |
| title_short |
Measurement of T-Lymphocyte Responses in Whole-Blood Cultures Using Newly Synthesized DNA and ATP |
| title_sort |
measurement of t-lymphocyte responses in whole-blood cultures using newly synthesized dna and atp |
| topic |
Microbiology (medical) Clinical Biochemistry Immunology Immunology and Allergy |
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http://dx.doi.org/10.1128/cdli.7.2.307-311.2000 |
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2000 |
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307-311 |
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<jats:title>ABSTRACT</jats:title>
<jats:p>
The proliferative response is most frequently determined by estimating the amount of [
<jats:sup>3</jats:sup>
H]thymidine incorporated into newly synthesized DNA. The [
<jats:sup>3</jats:sup>
H]thymidine procedure requires the use of radioisotopes as well as lengthy periods of incubation (>72 h). An alternative method of assessing T-lymphocyte activation in whole-blood cultures involves the measurement of the nucleotide ATP instead of [
<jats:sup>3</jats:sup>
H]thymidine incorporation. In addition, the Luminetics assay of T-cell activation measures specific T-lymphocyte subset responses through the use of paramagnetic particles coated with monoclonal antibodies against CD antigens. This assay permits rapid (24 h) analysis of lymphocyte subset activation responses to mitogens and recall antigens in small amounts of blood.
</jats:p> |
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| description | <jats:title>ABSTRACT</jats:title> <jats:p> The proliferative response is most frequently determined by estimating the amount of [ <jats:sup>3</jats:sup> H]thymidine incorporated into newly synthesized DNA. The [ <jats:sup>3</jats:sup> H]thymidine procedure requires the use of radioisotopes as well as lengthy periods of incubation (>72 h). An alternative method of assessing T-lymphocyte activation in whole-blood cultures involves the measurement of the nucleotide ATP instead of [ <jats:sup>3</jats:sup> H]thymidine incorporation. In addition, the Luminetics assay of T-cell activation measures specific T-lymphocyte subset responses through the use of paramagnetic particles coated with monoclonal antibodies against CD antigens. This assay permits rapid (24 h) analysis of lymphocyte subset activation responses to mitogens and recall antigens in small amounts of blood. </jats:p> |
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| spelling | Sottong, P. R. Rosebrock, J. A. Britz, J. A. Kramer, T. R. 1071-412X 1098-6588 American Society for Microbiology Microbiology (medical) Clinical Biochemistry Immunology Immunology and Allergy http://dx.doi.org/10.1128/cdli.7.2.307-311.2000 <jats:title>ABSTRACT</jats:title> <jats:p> The proliferative response is most frequently determined by estimating the amount of [ <jats:sup>3</jats:sup> H]thymidine incorporated into newly synthesized DNA. The [ <jats:sup>3</jats:sup> H]thymidine procedure requires the use of radioisotopes as well as lengthy periods of incubation (>72 h). An alternative method of assessing T-lymphocyte activation in whole-blood cultures involves the measurement of the nucleotide ATP instead of [ <jats:sup>3</jats:sup> H]thymidine incorporation. In addition, the Luminetics assay of T-cell activation measures specific T-lymphocyte subset responses through the use of paramagnetic particles coated with monoclonal antibodies against CD antigens. This assay permits rapid (24 h) analysis of lymphocyte subset activation responses to mitogens and recall antigens in small amounts of blood. </jats:p> Measurement of T-Lymphocyte Responses in Whole-Blood Cultures Using Newly Synthesized DNA and ATP Clinical Diagnostic Laboratory Immunology |
| spellingShingle | Sottong, P. R., Rosebrock, J. A., Britz, J. A., Kramer, T. R., Clinical Diagnostic Laboratory Immunology, Measurement of T-Lymphocyte Responses in Whole-Blood Cultures Using Newly Synthesized DNA and ATP, Microbiology (medical), Clinical Biochemistry, Immunology, Immunology and Allergy |
| title | Measurement of T-Lymphocyte Responses in Whole-Blood Cultures Using Newly Synthesized DNA and ATP |
| title_full | Measurement of T-Lymphocyte Responses in Whole-Blood Cultures Using Newly Synthesized DNA and ATP |
| title_fullStr | Measurement of T-Lymphocyte Responses in Whole-Blood Cultures Using Newly Synthesized DNA and ATP |
| title_full_unstemmed | Measurement of T-Lymphocyte Responses in Whole-Blood Cultures Using Newly Synthesized DNA and ATP |
| title_short | Measurement of T-Lymphocyte Responses in Whole-Blood Cultures Using Newly Synthesized DNA and ATP |
| title_sort | measurement of t-lymphocyte responses in whole-blood cultures using newly synthesized dna and atp |
| title_unstemmed | Measurement of T-Lymphocyte Responses in Whole-Blood Cultures Using Newly Synthesized DNA and ATP |
| topic | Microbiology (medical), Clinical Biochemistry, Immunology, Immunology and Allergy |
| url | http://dx.doi.org/10.1128/cdli.7.2.307-311.2000 |