Eintrag weiter verarbeiten
Role of the Regulatory GenerirAin the Transcriptional Response ofSinorhizobium melilotito Iron Limitation
Gespeichert in:
| Zeitschriftentitel: | Applied and Environmental Microbiology |
|---|---|
| Personen und Körperschaften: | , , , , |
| In: | Applied and Environmental Microbiology, 71, 2005, 10, S. 5969-5982 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Chao, Tzu-Chiao Buhrmester, Jens Hansmeier, Nicole Pühler, Alfred Weidner, Stefan Chao, Tzu-Chiao Buhrmester, Jens Hansmeier, Nicole Pühler, Alfred Weidner, Stefan |
|---|---|
| author |
Chao, Tzu-Chiao Buhrmester, Jens Hansmeier, Nicole Pühler, Alfred Weidner, Stefan |
| spellingShingle |
Chao, Tzu-Chiao Buhrmester, Jens Hansmeier, Nicole Pühler, Alfred Weidner, Stefan Applied and Environmental Microbiology Role of the Regulatory GenerirAin the Transcriptional Response ofSinorhizobium melilotito Iron Limitation Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
| author_sort |
chao, tzu-chiao |
| spelling |
Chao, Tzu-Chiao Buhrmester, Jens Hansmeier, Nicole Pühler, Alfred Weidner, Stefan 0099-2240 1098-5336 American Society for Microbiology Ecology Applied Microbiology and Biotechnology Food Science Biotechnology http://dx.doi.org/10.1128/aem.71.10.5969-5982.2005 <jats:title>ABSTRACT</jats:title><jats:p>A regulatory network of<jats:italic>Sinorhizobium meliloti</jats:italic>genes involved in adaptation to iron-limiting conditions and the involvement of the rhizobial iron regulator gene (<jats:italic>rirA</jats:italic>) were analyzed by mutation and microarray analyses. A constructed<jats:italic>S. meliloti rirA</jats:italic>mutant exhibited growth defects and enhanced H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub>sensitivity in the presence of iron, but symbiotic nitrogen fixation was not affected. To identify iron-responsive and RirA-regulated<jats:italic>S. meliloti</jats:italic>genes, a transcriptome approach using whole-genome microarrays was used. Altogether, 45 genes were found to be jointly derepressed by mutation of<jats:italic>rirA</jats:italic>and under different iron-limited conditions. As expected, a number of genes involved in iron transport (e.g.,<jats:italic>hmuPSTU</jats:italic>,<jats:italic>shmR</jats:italic>,<jats:italic>rhbABCDEF</jats:italic>,<jats:italic>rhtX</jats:italic>, and<jats:italic>rhtA</jats:italic>) and also genes with predicted functions in energy metabolism (e.g.,<jats:italic>fixN3</jats:italic>,<jats:italic>fixP3</jats:italic>, and<jats:italic>qxtAB</jats:italic>) and exopolysaccharide production (e.g.,<jats:italic>exoY</jats:italic>and<jats:italic>exoN</jats:italic>) were found in this group of genes. In addition, the iron deficiency response of<jats:italic>S. meliloti</jats:italic>also involved<jats:italic>rirA</jats:italic>-independent expression changes, including repression of the<jats:italic>S. meliloti</jats:italic>flagellar regulon. Finally, the RirA modulon also includes genes that are not iron responsive, including a gene cluster putatively involved in Fe-S cluster formation (<jats:italic>sufA</jats:italic>,<jats:italic>sufS</jats:italic>,<jats:italic>sufD</jats:italic>,<jats:italic>sufC</jats:italic>, and<jats:italic>sufB</jats:italic>).</jats:p> Role of the Regulatory Gene<i>rirA</i>in the Transcriptional Response of<i>Sinorhizobium meliloti</i>to Iron Limitation Applied and Environmental Microbiology |
| doi_str_mv |
10.1128/aem.71.10.5969-5982.2005 |
| facet_avail |
Online Free |
| finc_class_facet |
Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft Geographie Biologie Technik |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTEyOC9hZW0uNzEuMTAuNTk2OS01OTgyLjIwMDU |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTEyOC9hZW0uNzEuMTAuNTk2OS01OTgyLjIwMDU |
| institution |
DE-Pl11 DE-Rs1 DE-105 DE-14 DE-Ch1 DE-L229 DE-D275 DE-Bn3 DE-Brt1 DE-D161 DE-Zwi2 DE-Gla1 DE-Zi4 DE-15 |
| imprint |
American Society for Microbiology, 2005 |
| imprint_str_mv |
American Society for Microbiology, 2005 |
| issn |
0099-2240 1098-5336 |
| issn_str_mv |
0099-2240 1098-5336 |
| language |
English |
| mega_collection |
American Society for Microbiology (CrossRef) |
| match_str |
chao2005roleoftheregulatorygenerirainthetranscriptionalresponseofsinorhizobiummelilotitoironlimitation |
| publishDateSort |
2005 |
| publisher |
American Society for Microbiology |
| recordtype |
ai |
| record_format |
ai |
| series |
Applied and Environmental Microbiology |
| source_id |
49 |
| title |
Role of the Regulatory GenerirAin the Transcriptional Response ofSinorhizobium melilotito Iron Limitation |
| title_unstemmed |
Role of the Regulatory GenerirAin the Transcriptional Response ofSinorhizobium melilotito Iron Limitation |
| title_full |
Role of the Regulatory GenerirAin the Transcriptional Response ofSinorhizobium melilotito Iron Limitation |
| title_fullStr |
Role of the Regulatory GenerirAin the Transcriptional Response ofSinorhizobium melilotito Iron Limitation |
| title_full_unstemmed |
Role of the Regulatory GenerirAin the Transcriptional Response ofSinorhizobium melilotito Iron Limitation |
| title_short |
Role of the Regulatory GenerirAin the Transcriptional Response ofSinorhizobium melilotito Iron Limitation |
| title_sort |
role of the regulatory gene<i>rira</i>in the transcriptional response of<i>sinorhizobium meliloti</i>to iron limitation |
| topic |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
| url |
http://dx.doi.org/10.1128/aem.71.10.5969-5982.2005 |
| publishDate |
2005 |
| physical |
5969-5982 |
| description |
<jats:title>ABSTRACT</jats:title><jats:p>A regulatory network of<jats:italic>Sinorhizobium meliloti</jats:italic>genes involved in adaptation to iron-limiting conditions and the involvement of the rhizobial iron regulator gene (<jats:italic>rirA</jats:italic>) were analyzed by mutation and microarray analyses. A constructed<jats:italic>S. meliloti rirA</jats:italic>mutant exhibited growth defects and enhanced H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub>sensitivity in the presence of iron, but symbiotic nitrogen fixation was not affected. To identify iron-responsive and RirA-regulated<jats:italic>S. meliloti</jats:italic>genes, a transcriptome approach using whole-genome microarrays was used. Altogether, 45 genes were found to be jointly derepressed by mutation of<jats:italic>rirA</jats:italic>and under different iron-limited conditions. As expected, a number of genes involved in iron transport (e.g.,<jats:italic>hmuPSTU</jats:italic>,<jats:italic>shmR</jats:italic>,<jats:italic>rhbABCDEF</jats:italic>,<jats:italic>rhtX</jats:italic>, and<jats:italic>rhtA</jats:italic>) and also genes with predicted functions in energy metabolism (e.g.,<jats:italic>fixN3</jats:italic>,<jats:italic>fixP3</jats:italic>, and<jats:italic>qxtAB</jats:italic>) and exopolysaccharide production (e.g.,<jats:italic>exoY</jats:italic>and<jats:italic>exoN</jats:italic>) were found in this group of genes. In addition, the iron deficiency response of<jats:italic>S. meliloti</jats:italic>also involved<jats:italic>rirA</jats:italic>-independent expression changes, including repression of the<jats:italic>S. meliloti</jats:italic>flagellar regulon. Finally, the RirA modulon also includes genes that are not iron responsive, including a gene cluster putatively involved in Fe-S cluster formation (<jats:italic>sufA</jats:italic>,<jats:italic>sufS</jats:italic>,<jats:italic>sufD</jats:italic>,<jats:italic>sufC</jats:italic>, and<jats:italic>sufB</jats:italic>).</jats:p> |
| container_issue |
10 |
| container_start_page |
5969 |
| container_title |
Applied and Environmental Microbiology |
| container_volume |
71 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792345419979685902 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T17:23:11.571Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Role+of+the+Regulatory+GenerirAin+the+Transcriptional+Response+ofSinorhizobium+melilotito+Iron+Limitation&rft.date=2005-10-01&genre=article&issn=1098-5336&volume=71&issue=10&spage=5969&epage=5982&pages=5969-5982&jtitle=Applied+and+Environmental+Microbiology&atitle=Role+of+the+Regulatory+Gene%3Ci%3ErirA%3C%2Fi%3Ein+the+Transcriptional+Response+of%3Ci%3ESinorhizobium+meliloti%3C%2Fi%3Eto+Iron+Limitation&aulast=Weidner&aufirst=Stefan&rft_id=info%3Adoi%2F10.1128%2Faem.71.10.5969-5982.2005&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792345419979685902 |
| author | Chao, Tzu-Chiao, Buhrmester, Jens, Hansmeier, Nicole, Pühler, Alfred, Weidner, Stefan |
| author_facet | Chao, Tzu-Chiao, Buhrmester, Jens, Hansmeier, Nicole, Pühler, Alfred, Weidner, Stefan, Chao, Tzu-Chiao, Buhrmester, Jens, Hansmeier, Nicole, Pühler, Alfred, Weidner, Stefan |
| author_sort | chao, tzu-chiao |
| container_issue | 10 |
| container_start_page | 5969 |
| container_title | Applied and Environmental Microbiology |
| container_volume | 71 |
| description | <jats:title>ABSTRACT</jats:title><jats:p>A regulatory network of<jats:italic>Sinorhizobium meliloti</jats:italic>genes involved in adaptation to iron-limiting conditions and the involvement of the rhizobial iron regulator gene (<jats:italic>rirA</jats:italic>) were analyzed by mutation and microarray analyses. A constructed<jats:italic>S. meliloti rirA</jats:italic>mutant exhibited growth defects and enhanced H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub>sensitivity in the presence of iron, but symbiotic nitrogen fixation was not affected. To identify iron-responsive and RirA-regulated<jats:italic>S. meliloti</jats:italic>genes, a transcriptome approach using whole-genome microarrays was used. Altogether, 45 genes were found to be jointly derepressed by mutation of<jats:italic>rirA</jats:italic>and under different iron-limited conditions. As expected, a number of genes involved in iron transport (e.g.,<jats:italic>hmuPSTU</jats:italic>,<jats:italic>shmR</jats:italic>,<jats:italic>rhbABCDEF</jats:italic>,<jats:italic>rhtX</jats:italic>, and<jats:italic>rhtA</jats:italic>) and also genes with predicted functions in energy metabolism (e.g.,<jats:italic>fixN3</jats:italic>,<jats:italic>fixP3</jats:italic>, and<jats:italic>qxtAB</jats:italic>) and exopolysaccharide production (e.g.,<jats:italic>exoY</jats:italic>and<jats:italic>exoN</jats:italic>) were found in this group of genes. In addition, the iron deficiency response of<jats:italic>S. meliloti</jats:italic>also involved<jats:italic>rirA</jats:italic>-independent expression changes, including repression of the<jats:italic>S. meliloti</jats:italic>flagellar regulon. Finally, the RirA modulon also includes genes that are not iron responsive, including a gene cluster putatively involved in Fe-S cluster formation (<jats:italic>sufA</jats:italic>,<jats:italic>sufS</jats:italic>,<jats:italic>sufD</jats:italic>,<jats:italic>sufC</jats:italic>, and<jats:italic>sufB</jats:italic>).</jats:p> |
| doi_str_mv | 10.1128/aem.71.10.5969-5982.2005 |
| facet_avail | Online, Free |
| finc_class_facet | Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft, Geographie, Biologie, Technik |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTEyOC9hZW0uNzEuMTAuNTk2OS01OTgyLjIwMDU |
| imprint | American Society for Microbiology, 2005 |
| imprint_str_mv | American Society for Microbiology, 2005 |
| institution | DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-D161, DE-Zwi2, DE-Gla1, DE-Zi4, DE-15 |
| issn | 0099-2240, 1098-5336 |
| issn_str_mv | 0099-2240, 1098-5336 |
| language | English |
| last_indexed | 2024-03-01T17:23:11.571Z |
| match_str | chao2005roleoftheregulatorygenerirainthetranscriptionalresponseofsinorhizobiummelilotitoironlimitation |
| mega_collection | American Society for Microbiology (CrossRef) |
| physical | 5969-5982 |
| publishDate | 2005 |
| publishDateSort | 2005 |
| publisher | American Society for Microbiology |
| record_format | ai |
| recordtype | ai |
| series | Applied and Environmental Microbiology |
| source_id | 49 |
| spelling | Chao, Tzu-Chiao Buhrmester, Jens Hansmeier, Nicole Pühler, Alfred Weidner, Stefan 0099-2240 1098-5336 American Society for Microbiology Ecology Applied Microbiology and Biotechnology Food Science Biotechnology http://dx.doi.org/10.1128/aem.71.10.5969-5982.2005 <jats:title>ABSTRACT</jats:title><jats:p>A regulatory network of<jats:italic>Sinorhizobium meliloti</jats:italic>genes involved in adaptation to iron-limiting conditions and the involvement of the rhizobial iron regulator gene (<jats:italic>rirA</jats:italic>) were analyzed by mutation and microarray analyses. A constructed<jats:italic>S. meliloti rirA</jats:italic>mutant exhibited growth defects and enhanced H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub>sensitivity in the presence of iron, but symbiotic nitrogen fixation was not affected. To identify iron-responsive and RirA-regulated<jats:italic>S. meliloti</jats:italic>genes, a transcriptome approach using whole-genome microarrays was used. Altogether, 45 genes were found to be jointly derepressed by mutation of<jats:italic>rirA</jats:italic>and under different iron-limited conditions. As expected, a number of genes involved in iron transport (e.g.,<jats:italic>hmuPSTU</jats:italic>,<jats:italic>shmR</jats:italic>,<jats:italic>rhbABCDEF</jats:italic>,<jats:italic>rhtX</jats:italic>, and<jats:italic>rhtA</jats:italic>) and also genes with predicted functions in energy metabolism (e.g.,<jats:italic>fixN3</jats:italic>,<jats:italic>fixP3</jats:italic>, and<jats:italic>qxtAB</jats:italic>) and exopolysaccharide production (e.g.,<jats:italic>exoY</jats:italic>and<jats:italic>exoN</jats:italic>) were found in this group of genes. In addition, the iron deficiency response of<jats:italic>S. meliloti</jats:italic>also involved<jats:italic>rirA</jats:italic>-independent expression changes, including repression of the<jats:italic>S. meliloti</jats:italic>flagellar regulon. Finally, the RirA modulon also includes genes that are not iron responsive, including a gene cluster putatively involved in Fe-S cluster formation (<jats:italic>sufA</jats:italic>,<jats:italic>sufS</jats:italic>,<jats:italic>sufD</jats:italic>,<jats:italic>sufC</jats:italic>, and<jats:italic>sufB</jats:italic>).</jats:p> Role of the Regulatory Gene<i>rirA</i>in the Transcriptional Response of<i>Sinorhizobium meliloti</i>to Iron Limitation Applied and Environmental Microbiology |
| spellingShingle | Chao, Tzu-Chiao, Buhrmester, Jens, Hansmeier, Nicole, Pühler, Alfred, Weidner, Stefan, Applied and Environmental Microbiology, Role of the Regulatory GenerirAin the Transcriptional Response ofSinorhizobium melilotito Iron Limitation, Ecology, Applied Microbiology and Biotechnology, Food Science, Biotechnology |
| title | Role of the Regulatory GenerirAin the Transcriptional Response ofSinorhizobium melilotito Iron Limitation |
| title_full | Role of the Regulatory GenerirAin the Transcriptional Response ofSinorhizobium melilotito Iron Limitation |
| title_fullStr | Role of the Regulatory GenerirAin the Transcriptional Response ofSinorhizobium melilotito Iron Limitation |
| title_full_unstemmed | Role of the Regulatory GenerirAin the Transcriptional Response ofSinorhizobium melilotito Iron Limitation |
| title_short | Role of the Regulatory GenerirAin the Transcriptional Response ofSinorhizobium melilotito Iron Limitation |
| title_sort | role of the regulatory gene<i>rira</i>in the transcriptional response of<i>sinorhizobium meliloti</i>to iron limitation |
| title_unstemmed | Role of the Regulatory GenerirAin the Transcriptional Response ofSinorhizobium melilotito Iron Limitation |
| topic | Ecology, Applied Microbiology and Biotechnology, Food Science, Biotechnology |
| url | http://dx.doi.org/10.1128/aem.71.10.5969-5982.2005 |