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Improved Understanding of the Bacterial Vaginal Microbiota of Women before and after Probiotic Instillation
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| Zeitschriftentitel: | Applied and Environmental Microbiology |
|---|---|
| Personen und Körperschaften: | , , |
| In: | Applied and Environmental Microbiology, 69, 2003, 1, S. 97-101 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Burton, Jeremy P. Cadieux, Peter A. Reid, Gregor Burton, Jeremy P. Cadieux, Peter A. Reid, Gregor |
|---|---|
| author |
Burton, Jeremy P. Cadieux, Peter A. Reid, Gregor |
| spellingShingle |
Burton, Jeremy P. Cadieux, Peter A. Reid, Gregor Applied and Environmental Microbiology Improved Understanding of the Bacterial Vaginal Microbiota of Women before and after Probiotic Instillation Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
| author_sort |
burton, jeremy p. |
| spelling |
Burton, Jeremy P. Cadieux, Peter A. Reid, Gregor 0099-2240 1098-5336 American Society for Microbiology Ecology Applied Microbiology and Biotechnology Food Science Biotechnology http://dx.doi.org/10.1128/aem.69.1.97-101.2003 <jats:title>ABSTRACT</jats:title> <jats:p> The vaginal bacterial microbiota of 19 premenopausal women was examined by PCR-denaturing gradient gel electrophoresis (DGGE) and sequencing of the V2-V3 region of the 16S rRNA gene. Ten of the women were studied further to investigate the effect and persistence of vaginally inserted capsules containing viable lactobacilli. PCR-DGGE indicated that most subjects had a microbiota represented by one to three dominant DNA fragments. Analysis of these fragments revealed that 79% of the women possessed sequences with high levels of similarity to <jats:italic>Lactobacillus</jats:italic> species sequences. Sequences homologous to <jats:italic>Lactobacillus iners</jats:italic> sequences were the most common and were detected in 42% of the women tested. Alteration of the vaginal microbiota could be detected by PCR-DGGE in several women after the instillation of lactobacilli. Additionally, randomly amplified polymorphic DNA analysis of lactobacilli isolated from selective media demonstrated that the exogenous strains could be detected for up to 21 days in some subjects. This study demonstrates that non-culture-based techniques, such as PCR-DGGE, are useful adjuncts for studies of the vaginal microbiota. </jats:p> Improved Understanding of the Bacterial Vaginal Microbiota of Women before and after Probiotic Instillation Applied and Environmental Microbiology |
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| title |
Improved Understanding of the Bacterial Vaginal Microbiota of Women before and after Probiotic Instillation |
| title_unstemmed |
Improved Understanding of the Bacterial Vaginal Microbiota of Women before and after Probiotic Instillation |
| title_full |
Improved Understanding of the Bacterial Vaginal Microbiota of Women before and after Probiotic Instillation |
| title_fullStr |
Improved Understanding of the Bacterial Vaginal Microbiota of Women before and after Probiotic Instillation |
| title_full_unstemmed |
Improved Understanding of the Bacterial Vaginal Microbiota of Women before and after Probiotic Instillation |
| title_short |
Improved Understanding of the Bacterial Vaginal Microbiota of Women before and after Probiotic Instillation |
| title_sort |
improved understanding of the bacterial vaginal microbiota of women before and after probiotic instillation |
| topic |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
| url |
http://dx.doi.org/10.1128/aem.69.1.97-101.2003 |
| publishDate |
2003 |
| physical |
97-101 |
| description |
<jats:title>ABSTRACT</jats:title>
<jats:p>
The vaginal bacterial microbiota of 19 premenopausal women was examined by PCR-denaturing gradient gel electrophoresis (DGGE) and sequencing of the V2-V3 region of the 16S rRNA gene. Ten of the women were studied further to investigate the effect and persistence of vaginally inserted capsules containing viable lactobacilli. PCR-DGGE indicated that most subjects had a microbiota represented by one to three dominant DNA fragments. Analysis of these fragments revealed that 79% of the women possessed sequences with high levels of similarity to
<jats:italic>Lactobacillus</jats:italic>
species sequences. Sequences homologous to
<jats:italic>Lactobacillus iners</jats:italic>
sequences were the most common and were detected in 42% of the women tested. Alteration of the vaginal microbiota could be detected by PCR-DGGE in several women after the instillation of lactobacilli. Additionally, randomly amplified polymorphic DNA analysis of lactobacilli isolated from selective media demonstrated that the exogenous strains could be detected for up to 21 days in some subjects. This study demonstrates that non-culture-based techniques, such as PCR-DGGE, are useful adjuncts for studies of the vaginal microbiota.
</jats:p> |
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| description | <jats:title>ABSTRACT</jats:title> <jats:p> The vaginal bacterial microbiota of 19 premenopausal women was examined by PCR-denaturing gradient gel electrophoresis (DGGE) and sequencing of the V2-V3 region of the 16S rRNA gene. Ten of the women were studied further to investigate the effect and persistence of vaginally inserted capsules containing viable lactobacilli. PCR-DGGE indicated that most subjects had a microbiota represented by one to three dominant DNA fragments. Analysis of these fragments revealed that 79% of the women possessed sequences with high levels of similarity to <jats:italic>Lactobacillus</jats:italic> species sequences. Sequences homologous to <jats:italic>Lactobacillus iners</jats:italic> sequences were the most common and were detected in 42% of the women tested. Alteration of the vaginal microbiota could be detected by PCR-DGGE in several women after the instillation of lactobacilli. Additionally, randomly amplified polymorphic DNA analysis of lactobacilli isolated from selective media demonstrated that the exogenous strains could be detected for up to 21 days in some subjects. This study demonstrates that non-culture-based techniques, such as PCR-DGGE, are useful adjuncts for studies of the vaginal microbiota. </jats:p> |
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| spelling | Burton, Jeremy P. Cadieux, Peter A. Reid, Gregor 0099-2240 1098-5336 American Society for Microbiology Ecology Applied Microbiology and Biotechnology Food Science Biotechnology http://dx.doi.org/10.1128/aem.69.1.97-101.2003 <jats:title>ABSTRACT</jats:title> <jats:p> The vaginal bacterial microbiota of 19 premenopausal women was examined by PCR-denaturing gradient gel electrophoresis (DGGE) and sequencing of the V2-V3 region of the 16S rRNA gene. Ten of the women were studied further to investigate the effect and persistence of vaginally inserted capsules containing viable lactobacilli. PCR-DGGE indicated that most subjects had a microbiota represented by one to three dominant DNA fragments. Analysis of these fragments revealed that 79% of the women possessed sequences with high levels of similarity to <jats:italic>Lactobacillus</jats:italic> species sequences. Sequences homologous to <jats:italic>Lactobacillus iners</jats:italic> sequences were the most common and were detected in 42% of the women tested. Alteration of the vaginal microbiota could be detected by PCR-DGGE in several women after the instillation of lactobacilli. Additionally, randomly amplified polymorphic DNA analysis of lactobacilli isolated from selective media demonstrated that the exogenous strains could be detected for up to 21 days in some subjects. This study demonstrates that non-culture-based techniques, such as PCR-DGGE, are useful adjuncts for studies of the vaginal microbiota. </jats:p> Improved Understanding of the Bacterial Vaginal Microbiota of Women before and after Probiotic Instillation Applied and Environmental Microbiology |
| spellingShingle | Burton, Jeremy P., Cadieux, Peter A., Reid, Gregor, Applied and Environmental Microbiology, Improved Understanding of the Bacterial Vaginal Microbiota of Women before and after Probiotic Instillation, Ecology, Applied Microbiology and Biotechnology, Food Science, Biotechnology |
| title | Improved Understanding of the Bacterial Vaginal Microbiota of Women before and after Probiotic Instillation |
| title_full | Improved Understanding of the Bacterial Vaginal Microbiota of Women before and after Probiotic Instillation |
| title_fullStr | Improved Understanding of the Bacterial Vaginal Microbiota of Women before and after Probiotic Instillation |
| title_full_unstemmed | Improved Understanding of the Bacterial Vaginal Microbiota of Women before and after Probiotic Instillation |
| title_short | Improved Understanding of the Bacterial Vaginal Microbiota of Women before and after Probiotic Instillation |
| title_sort | improved understanding of the bacterial vaginal microbiota of women before and after probiotic instillation |
| title_unstemmed | Improved Understanding of the Bacterial Vaginal Microbiota of Women before and after Probiotic Instillation |
| topic | Ecology, Applied Microbiology and Biotechnology, Food Science, Biotechnology |
| url | http://dx.doi.org/10.1128/aem.69.1.97-101.2003 |