Eintrag weiter verarbeiten
Rapid Immunoassays for Detection of UV-Induced Cyclobutane Pyrimidine Dimers in Whole Bacterial Cells
Gespeichert in:
| Zeitschriftentitel: | Applied and Environmental Microbiology |
|---|---|
| Personen und Körperschaften: | , |
| In: | Applied and Environmental Microbiology, 68, 2002, 5, S. 2542-2549 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Peccia, Jordan Hernandez, Mark Peccia, Jordan Hernandez, Mark |
|---|---|
| author |
Peccia, Jordan Hernandez, Mark |
| spellingShingle |
Peccia, Jordan Hernandez, Mark Applied and Environmental Microbiology Rapid Immunoassays for Detection of UV-Induced Cyclobutane Pyrimidine Dimers in Whole Bacterial Cells Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
| author_sort |
peccia, jordan |
| spelling |
Peccia, Jordan Hernandez, Mark 0099-2240 1098-5336 American Society for Microbiology Ecology Applied Microbiology and Biotechnology Food Science Biotechnology http://dx.doi.org/10.1128/aem.68.5.2542-2549.2002 <jats:title>ABSTRACT</jats:title> <jats:p> Immunoassays were developed to measure DNA damage retained by UV-irradiated whole bacterial cells. Active <jats:italic>Mycobacterium parafortuitum</jats:italic> and <jats:italic>Serratia marcescens</jats:italic> cells were fixed and incubated with cyclobutane pyrimidine dimer-binding antibodies after being exposed to known UV doses (254 nm). When both fluorescent (Alexa Fluor 488) and radiolabeled ( <jats:sup>125</jats:sup> I) secondary antibodies were used as reporters, indirect whole-cell assays were sensitive enough to measure intracellular UV photoproducts in <jats:italic>M. parafortuitum</jats:italic> and <jats:italic>S. marcescens</jats:italic> cells as well as photoenzymatic repair responses in <jats:italic>S. marcescens</jats:italic> cells. For the same UV dose, fluorescent DNA photoproduct detection limits in whole-cell assays (immunofluorescent microscopy) were similar to those in fluorescent assays performed on membrane-bound DNA extracts (immunoslot blot). With either fluorescent or radiolabeled reporters, the intracellular cyclobutane pyrimidine dimer content of UV-irradiated whole bacterial cells could be reliably quantified after undergoing a <0.5-order-of-magnitude decrease in culturability. Immunofluorescent microscopy results showed that photoenzymatic repair competence is not uniformly distributed among exponential-growth UV-irradiated pure cultures. </jats:p> Rapid Immunoassays for Detection of UV-Induced Cyclobutane Pyrimidine Dimers in Whole Bacterial Cells Applied and Environmental Microbiology |
| doi_str_mv |
10.1128/aem.68.5.2542-2549.2002 |
| facet_avail |
Online Free |
| finc_class_facet |
Geographie Biologie Technik Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTEyOC9hZW0uNjguNS4yNTQyLTI1NDkuMjAwMg |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTEyOC9hZW0uNjguNS4yNTQyLTI1NDkuMjAwMg |
| institution |
DE-105 DE-14 DE-Ch1 DE-L229 DE-D275 DE-Bn3 DE-Brt1 DE-Zwi2 DE-D161 DE-Gla1 DE-Zi4 DE-15 DE-Pl11 DE-Rs1 |
| imprint |
American Society for Microbiology, 2002 |
| imprint_str_mv |
American Society for Microbiology, 2002 |
| issn |
0099-2240 1098-5336 |
| issn_str_mv |
0099-2240 1098-5336 |
| language |
English |
| mega_collection |
American Society for Microbiology (CrossRef) |
| match_str |
peccia2002rapidimmunoassaysfordetectionofuvinducedcyclobutanepyrimidinedimersinwholebacterialcells |
| publishDateSort |
2002 |
| publisher |
American Society for Microbiology |
| recordtype |
ai |
| record_format |
ai |
| series |
Applied and Environmental Microbiology |
| source_id |
49 |
| title |
Rapid Immunoassays for Detection of UV-Induced Cyclobutane Pyrimidine Dimers in Whole Bacterial Cells |
| title_unstemmed |
Rapid Immunoassays for Detection of UV-Induced Cyclobutane Pyrimidine Dimers in Whole Bacterial Cells |
| title_full |
Rapid Immunoassays for Detection of UV-Induced Cyclobutane Pyrimidine Dimers in Whole Bacterial Cells |
| title_fullStr |
Rapid Immunoassays for Detection of UV-Induced Cyclobutane Pyrimidine Dimers in Whole Bacterial Cells |
| title_full_unstemmed |
Rapid Immunoassays for Detection of UV-Induced Cyclobutane Pyrimidine Dimers in Whole Bacterial Cells |
| title_short |
Rapid Immunoassays for Detection of UV-Induced Cyclobutane Pyrimidine Dimers in Whole Bacterial Cells |
| title_sort |
rapid immunoassays for detection of uv-induced cyclobutane pyrimidine dimers in whole bacterial cells |
| topic |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
| url |
http://dx.doi.org/10.1128/aem.68.5.2542-2549.2002 |
| publishDate |
2002 |
| physical |
2542-2549 |
| description |
<jats:title>ABSTRACT</jats:title>
<jats:p>
Immunoassays were developed to measure DNA damage retained by UV-irradiated whole bacterial cells. Active
<jats:italic>Mycobacterium parafortuitum</jats:italic>
and
<jats:italic>Serratia marcescens</jats:italic>
cells were fixed and incubated with cyclobutane pyrimidine dimer-binding antibodies after being exposed to known UV doses (254 nm). When both fluorescent (Alexa Fluor 488) and radiolabeled (
<jats:sup>125</jats:sup>
I) secondary antibodies were used as reporters, indirect whole-cell assays were sensitive enough to measure intracellular UV photoproducts in
<jats:italic>M. parafortuitum</jats:italic>
and
<jats:italic>S. marcescens</jats:italic>
cells as well as photoenzymatic repair responses in
<jats:italic>S. marcescens</jats:italic>
cells. For the same UV dose, fluorescent DNA photoproduct detection limits in whole-cell assays (immunofluorescent microscopy) were similar to those in fluorescent assays performed on membrane-bound DNA extracts (immunoslot blot). With either fluorescent or radiolabeled reporters, the intracellular cyclobutane pyrimidine dimer content of UV-irradiated whole bacterial cells could be reliably quantified after undergoing a <0.5-order-of-magnitude decrease in culturability. Immunofluorescent microscopy results showed that photoenzymatic repair competence is not uniformly distributed among exponential-growth UV-irradiated pure cultures.
</jats:p> |
| container_issue |
5 |
| container_start_page |
2542 |
| container_title |
Applied and Environmental Microbiology |
| container_volume |
68 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792335743546294272 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T14:49:21.612Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Rapid+Immunoassays+for+Detection+of+UV-Induced+Cyclobutane+Pyrimidine+Dimers+in+Whole+Bacterial+Cells&rft.date=2002-05-01&genre=article&issn=1098-5336&volume=68&issue=5&spage=2542&epage=2549&pages=2542-2549&jtitle=Applied+and+Environmental+Microbiology&atitle=Rapid+Immunoassays+for+Detection+of+UV-Induced+Cyclobutane+Pyrimidine+Dimers+in+Whole+Bacterial+Cells&aulast=Hernandez&aufirst=Mark&rft_id=info%3Adoi%2F10.1128%2Faem.68.5.2542-2549.2002&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792335743546294272 |
| author | Peccia, Jordan, Hernandez, Mark |
| author_facet | Peccia, Jordan, Hernandez, Mark, Peccia, Jordan, Hernandez, Mark |
| author_sort | peccia, jordan |
| container_issue | 5 |
| container_start_page | 2542 |
| container_title | Applied and Environmental Microbiology |
| container_volume | 68 |
| description | <jats:title>ABSTRACT</jats:title> <jats:p> Immunoassays were developed to measure DNA damage retained by UV-irradiated whole bacterial cells. Active <jats:italic>Mycobacterium parafortuitum</jats:italic> and <jats:italic>Serratia marcescens</jats:italic> cells were fixed and incubated with cyclobutane pyrimidine dimer-binding antibodies after being exposed to known UV doses (254 nm). When both fluorescent (Alexa Fluor 488) and radiolabeled ( <jats:sup>125</jats:sup> I) secondary antibodies were used as reporters, indirect whole-cell assays were sensitive enough to measure intracellular UV photoproducts in <jats:italic>M. parafortuitum</jats:italic> and <jats:italic>S. marcescens</jats:italic> cells as well as photoenzymatic repair responses in <jats:italic>S. marcescens</jats:italic> cells. For the same UV dose, fluorescent DNA photoproduct detection limits in whole-cell assays (immunofluorescent microscopy) were similar to those in fluorescent assays performed on membrane-bound DNA extracts (immunoslot blot). With either fluorescent or radiolabeled reporters, the intracellular cyclobutane pyrimidine dimer content of UV-irradiated whole bacterial cells could be reliably quantified after undergoing a <0.5-order-of-magnitude decrease in culturability. Immunofluorescent microscopy results showed that photoenzymatic repair competence is not uniformly distributed among exponential-growth UV-irradiated pure cultures. </jats:p> |
| doi_str_mv | 10.1128/aem.68.5.2542-2549.2002 |
| facet_avail | Online, Free |
| finc_class_facet | Geographie, Biologie, Technik, Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTEyOC9hZW0uNjguNS4yNTQyLTI1NDkuMjAwMg |
| imprint | American Society for Microbiology, 2002 |
| imprint_str_mv | American Society for Microbiology, 2002 |
| institution | DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1 |
| issn | 0099-2240, 1098-5336 |
| issn_str_mv | 0099-2240, 1098-5336 |
| language | English |
| last_indexed | 2024-03-01T14:49:21.612Z |
| match_str | peccia2002rapidimmunoassaysfordetectionofuvinducedcyclobutanepyrimidinedimersinwholebacterialcells |
| mega_collection | American Society for Microbiology (CrossRef) |
| physical | 2542-2549 |
| publishDate | 2002 |
| publishDateSort | 2002 |
| publisher | American Society for Microbiology |
| record_format | ai |
| recordtype | ai |
| series | Applied and Environmental Microbiology |
| source_id | 49 |
| spelling | Peccia, Jordan Hernandez, Mark 0099-2240 1098-5336 American Society for Microbiology Ecology Applied Microbiology and Biotechnology Food Science Biotechnology http://dx.doi.org/10.1128/aem.68.5.2542-2549.2002 <jats:title>ABSTRACT</jats:title> <jats:p> Immunoassays were developed to measure DNA damage retained by UV-irradiated whole bacterial cells. Active <jats:italic>Mycobacterium parafortuitum</jats:italic> and <jats:italic>Serratia marcescens</jats:italic> cells were fixed and incubated with cyclobutane pyrimidine dimer-binding antibodies after being exposed to known UV doses (254 nm). When both fluorescent (Alexa Fluor 488) and radiolabeled ( <jats:sup>125</jats:sup> I) secondary antibodies were used as reporters, indirect whole-cell assays were sensitive enough to measure intracellular UV photoproducts in <jats:italic>M. parafortuitum</jats:italic> and <jats:italic>S. marcescens</jats:italic> cells as well as photoenzymatic repair responses in <jats:italic>S. marcescens</jats:italic> cells. For the same UV dose, fluorescent DNA photoproduct detection limits in whole-cell assays (immunofluorescent microscopy) were similar to those in fluorescent assays performed on membrane-bound DNA extracts (immunoslot blot). With either fluorescent or radiolabeled reporters, the intracellular cyclobutane pyrimidine dimer content of UV-irradiated whole bacterial cells could be reliably quantified after undergoing a <0.5-order-of-magnitude decrease in culturability. Immunofluorescent microscopy results showed that photoenzymatic repair competence is not uniformly distributed among exponential-growth UV-irradiated pure cultures. </jats:p> Rapid Immunoassays for Detection of UV-Induced Cyclobutane Pyrimidine Dimers in Whole Bacterial Cells Applied and Environmental Microbiology |
| spellingShingle | Peccia, Jordan, Hernandez, Mark, Applied and Environmental Microbiology, Rapid Immunoassays for Detection of UV-Induced Cyclobutane Pyrimidine Dimers in Whole Bacterial Cells, Ecology, Applied Microbiology and Biotechnology, Food Science, Biotechnology |
| title | Rapid Immunoassays for Detection of UV-Induced Cyclobutane Pyrimidine Dimers in Whole Bacterial Cells |
| title_full | Rapid Immunoassays for Detection of UV-Induced Cyclobutane Pyrimidine Dimers in Whole Bacterial Cells |
| title_fullStr | Rapid Immunoassays for Detection of UV-Induced Cyclobutane Pyrimidine Dimers in Whole Bacterial Cells |
| title_full_unstemmed | Rapid Immunoassays for Detection of UV-Induced Cyclobutane Pyrimidine Dimers in Whole Bacterial Cells |
| title_short | Rapid Immunoassays for Detection of UV-Induced Cyclobutane Pyrimidine Dimers in Whole Bacterial Cells |
| title_sort | rapid immunoassays for detection of uv-induced cyclobutane pyrimidine dimers in whole bacterial cells |
| title_unstemmed | Rapid Immunoassays for Detection of UV-Induced Cyclobutane Pyrimidine Dimers in Whole Bacterial Cells |
| topic | Ecology, Applied Microbiology and Biotechnology, Food Science, Biotechnology |
| url | http://dx.doi.org/10.1128/aem.68.5.2542-2549.2002 |