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Microbial Composition of Near-Boiling Silica-Depositing Thermal Springs throughout Yellowstone National Park
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| Zeitschriftentitel: | Applied and Environmental Microbiology |
|---|---|
| Personen und Körperschaften: | , , |
| In: | Applied and Environmental Microbiology, 68, 2002, 10, S. 5123-5135 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
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| Schlagwörter: |
| author_facet |
Blank, Carrine E. Cady, Sherry L. Pace, Norman R. Blank, Carrine E. Cady, Sherry L. Pace, Norman R. |
|---|---|
| author |
Blank, Carrine E. Cady, Sherry L. Pace, Norman R. |
| spellingShingle |
Blank, Carrine E. Cady, Sherry L. Pace, Norman R. Applied and Environmental Microbiology Microbial Composition of Near-Boiling Silica-Depositing Thermal Springs throughout Yellowstone National Park Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
| author_sort |
blank, carrine e. |
| spelling |
Blank, Carrine E. Cady, Sherry L. Pace, Norman R. 0099-2240 1098-5336 American Society for Microbiology Ecology Applied Microbiology and Biotechnology Food Science Biotechnology http://dx.doi.org/10.1128/aem.68.10.5123-5135.2002 <jats:title>ABSTRACT</jats:title> <jats:p> The extent of hyperthermophilic microbial diversity associated with siliceous sinter (geyserite) was characterized in seven near-boiling silica-depositing springs throughout Yellowstone National Park using environmental PCR amplification of small-subunit rRNA genes (SSU rDNA), large-subunit rDNA, and the internal transcribed spacer (ITS). We found that <jats:italic>Thermocrinis ruber</jats:italic> , a member of the order <jats:italic>Aquificales</jats:italic> , is ubiquitous, an indication that primary production in these springs is driven by hydrogen oxidation. Several other lineages with no known close relatives were identified that branch among the hyperthermophilic bacteria. Although they all branch deep in the bacterial tree, the precise phylogenetic placement of many of these lineages is unresolved at this time. While some springs contained a fair amount of phylogenetic diversity, others did not. Within the same spring, communities in the subaqueous environment were not appreciably different than those in the splash zone at the edge of the pool, although a greater number of phylotypes was found along the pool's edge. Also, microbial community composition appeared to have little correlation with the type of sinter morphology. The number of cell morphotypes identified by fluorescence in situ hybridization and scanning electron microscopy was greater than the number of phylotypes in SSU clone libraries. Despite little variation in <jats:italic>Thermocrinis ruber</jats:italic> SSU sequences, abundant variation was found in the hypervariable ITS region. The distribution of ITS sequence types appeared to be correlated with distinct morphotypes of <jats:italic>Thermocrinis ruber</jats:italic> in different pools. Therefore, species- or subspecies-level divergences are present but not detectable in highly conserved SSU sequences. </jats:p> Microbial Composition of Near-Boiling Silica-Depositing Thermal Springs throughout Yellowstone National Park Applied and Environmental Microbiology |
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| title |
Microbial Composition of Near-Boiling Silica-Depositing Thermal Springs throughout Yellowstone National Park |
| title_unstemmed |
Microbial Composition of Near-Boiling Silica-Depositing Thermal Springs throughout Yellowstone National Park |
| title_full |
Microbial Composition of Near-Boiling Silica-Depositing Thermal Springs throughout Yellowstone National Park |
| title_fullStr |
Microbial Composition of Near-Boiling Silica-Depositing Thermal Springs throughout Yellowstone National Park |
| title_full_unstemmed |
Microbial Composition of Near-Boiling Silica-Depositing Thermal Springs throughout Yellowstone National Park |
| title_short |
Microbial Composition of Near-Boiling Silica-Depositing Thermal Springs throughout Yellowstone National Park |
| title_sort |
microbial composition of near-boiling silica-depositing thermal springs throughout yellowstone national park |
| topic |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
| url |
http://dx.doi.org/10.1128/aem.68.10.5123-5135.2002 |
| publishDate |
2002 |
| physical |
5123-5135 |
| description |
<jats:title>ABSTRACT</jats:title>
<jats:p>
The extent of hyperthermophilic microbial diversity associated with siliceous sinter (geyserite) was characterized in seven near-boiling silica-depositing springs throughout Yellowstone National Park using environmental PCR amplification of small-subunit rRNA genes (SSU rDNA), large-subunit rDNA, and the internal transcribed spacer (ITS). We found that
<jats:italic>Thermocrinis ruber</jats:italic>
, a member of the order
<jats:italic>Aquificales</jats:italic>
, is ubiquitous, an indication that primary production in these springs is driven by hydrogen oxidation. Several other lineages with no known close relatives were identified that branch among the hyperthermophilic bacteria. Although they all branch deep in the bacterial tree, the precise phylogenetic placement of many of these lineages is unresolved at this time. While some springs contained a fair amount of phylogenetic diversity, others did not. Within the same spring, communities in the subaqueous environment were not appreciably different than those in the splash zone at the edge of the pool, although a greater number of phylotypes was found along the pool's edge. Also, microbial community composition appeared to have little correlation with the type of sinter morphology. The number of cell morphotypes identified by fluorescence in situ hybridization and scanning electron microscopy was greater than the number of phylotypes in SSU clone libraries. Despite little variation in
<jats:italic>Thermocrinis ruber</jats:italic>
SSU sequences, abundant variation was found in the hypervariable ITS region. The distribution of ITS sequence types appeared to be correlated with distinct morphotypes of
<jats:italic>Thermocrinis ruber</jats:italic>
in different pools. Therefore, species- or subspecies-level divergences are present but not detectable in highly conserved SSU sequences.
</jats:p> |
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| author | Blank, Carrine E., Cady, Sherry L., Pace, Norman R. |
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| description | <jats:title>ABSTRACT</jats:title> <jats:p> The extent of hyperthermophilic microbial diversity associated with siliceous sinter (geyserite) was characterized in seven near-boiling silica-depositing springs throughout Yellowstone National Park using environmental PCR amplification of small-subunit rRNA genes (SSU rDNA), large-subunit rDNA, and the internal transcribed spacer (ITS). We found that <jats:italic>Thermocrinis ruber</jats:italic> , a member of the order <jats:italic>Aquificales</jats:italic> , is ubiquitous, an indication that primary production in these springs is driven by hydrogen oxidation. Several other lineages with no known close relatives were identified that branch among the hyperthermophilic bacteria. Although they all branch deep in the bacterial tree, the precise phylogenetic placement of many of these lineages is unresolved at this time. While some springs contained a fair amount of phylogenetic diversity, others did not. Within the same spring, communities in the subaqueous environment were not appreciably different than those in the splash zone at the edge of the pool, although a greater number of phylotypes was found along the pool's edge. Also, microbial community composition appeared to have little correlation with the type of sinter morphology. The number of cell morphotypes identified by fluorescence in situ hybridization and scanning electron microscopy was greater than the number of phylotypes in SSU clone libraries. Despite little variation in <jats:italic>Thermocrinis ruber</jats:italic> SSU sequences, abundant variation was found in the hypervariable ITS region. The distribution of ITS sequence types appeared to be correlated with distinct morphotypes of <jats:italic>Thermocrinis ruber</jats:italic> in different pools. Therefore, species- or subspecies-level divergences are present but not detectable in highly conserved SSU sequences. </jats:p> |
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| imprint_str_mv | American Society for Microbiology, 2002 |
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| spelling | Blank, Carrine E. Cady, Sherry L. Pace, Norman R. 0099-2240 1098-5336 American Society for Microbiology Ecology Applied Microbiology and Biotechnology Food Science Biotechnology http://dx.doi.org/10.1128/aem.68.10.5123-5135.2002 <jats:title>ABSTRACT</jats:title> <jats:p> The extent of hyperthermophilic microbial diversity associated with siliceous sinter (geyserite) was characterized in seven near-boiling silica-depositing springs throughout Yellowstone National Park using environmental PCR amplification of small-subunit rRNA genes (SSU rDNA), large-subunit rDNA, and the internal transcribed spacer (ITS). We found that <jats:italic>Thermocrinis ruber</jats:italic> , a member of the order <jats:italic>Aquificales</jats:italic> , is ubiquitous, an indication that primary production in these springs is driven by hydrogen oxidation. Several other lineages with no known close relatives were identified that branch among the hyperthermophilic bacteria. Although they all branch deep in the bacterial tree, the precise phylogenetic placement of many of these lineages is unresolved at this time. While some springs contained a fair amount of phylogenetic diversity, others did not. Within the same spring, communities in the subaqueous environment were not appreciably different than those in the splash zone at the edge of the pool, although a greater number of phylotypes was found along the pool's edge. Also, microbial community composition appeared to have little correlation with the type of sinter morphology. The number of cell morphotypes identified by fluorescence in situ hybridization and scanning electron microscopy was greater than the number of phylotypes in SSU clone libraries. Despite little variation in <jats:italic>Thermocrinis ruber</jats:italic> SSU sequences, abundant variation was found in the hypervariable ITS region. The distribution of ITS sequence types appeared to be correlated with distinct morphotypes of <jats:italic>Thermocrinis ruber</jats:italic> in different pools. Therefore, species- or subspecies-level divergences are present but not detectable in highly conserved SSU sequences. </jats:p> Microbial Composition of Near-Boiling Silica-Depositing Thermal Springs throughout Yellowstone National Park Applied and Environmental Microbiology |
| spellingShingle | Blank, Carrine E., Cady, Sherry L., Pace, Norman R., Applied and Environmental Microbiology, Microbial Composition of Near-Boiling Silica-Depositing Thermal Springs throughout Yellowstone National Park, Ecology, Applied Microbiology and Biotechnology, Food Science, Biotechnology |
| title | Microbial Composition of Near-Boiling Silica-Depositing Thermal Springs throughout Yellowstone National Park |
| title_full | Microbial Composition of Near-Boiling Silica-Depositing Thermal Springs throughout Yellowstone National Park |
| title_fullStr | Microbial Composition of Near-Boiling Silica-Depositing Thermal Springs throughout Yellowstone National Park |
| title_full_unstemmed | Microbial Composition of Near-Boiling Silica-Depositing Thermal Springs throughout Yellowstone National Park |
| title_short | Microbial Composition of Near-Boiling Silica-Depositing Thermal Springs throughout Yellowstone National Park |
| title_sort | microbial composition of near-boiling silica-depositing thermal springs throughout yellowstone national park |
| title_unstemmed | Microbial Composition of Near-Boiling Silica-Depositing Thermal Springs throughout Yellowstone National Park |
| topic | Ecology, Applied Microbiology and Biotechnology, Food Science, Biotechnology |
| url | http://dx.doi.org/10.1128/aem.68.10.5123-5135.2002 |