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Citrate Metabolism by Enterococcus faecalis FAIR-E 229
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| Zeitschriftentitel: | Applied and Environmental Microbiology |
|---|---|
| Personen und Körperschaften: | , , |
| In: | Applied and Environmental Microbiology, 67, 2001, 12, S. 5482-5487 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Sarantinopoulos, Panagiotis Kalantzopoulos, George Tsakalidou, Effie Sarantinopoulos, Panagiotis Kalantzopoulos, George Tsakalidou, Effie |
|---|---|
| author |
Sarantinopoulos, Panagiotis Kalantzopoulos, George Tsakalidou, Effie |
| spellingShingle |
Sarantinopoulos, Panagiotis Kalantzopoulos, George Tsakalidou, Effie Applied and Environmental Microbiology Citrate Metabolism by Enterococcus faecalis FAIR-E 229 Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
| author_sort |
sarantinopoulos, panagiotis |
| spelling |
Sarantinopoulos, Panagiotis Kalantzopoulos, George Tsakalidou, Effie 0099-2240 1098-5336 American Society for Microbiology Ecology Applied Microbiology and Biotechnology Food Science Biotechnology http://dx.doi.org/10.1128/aem.67.12.5482-5487.2001 <jats:title>ABSTRACT</jats:title> <jats:p> Citrate metabolism by <jats:italic>Enterococcus faecalis</jats:italic> FAIR-E 229 was studied in various growth media containing citrate either in the presence of glucose or lactose or as the sole carbon source. In skim milk (130 mM lactose, 8 mM citrate), cometabolism of citrate and lactose was observed from the first stages of the growth phase. Lactose was stoichiometrically converted into lactate, while citrate was converted into acetate, formate, and ethanol. When de Man-Rogosa-Sharpe (MRS) broth containing lactose (28 mM) instead of glucose was used, <jats:italic>E. faecalis</jats:italic> FAIR-E 229 catabolized only the carbohydrate. Lactate was the major end product, and small amounts of ethanol were also detected. Increasing concentrations of citrate (10, 40, 70, and 100 mM) added to MRS broth enhanced both the maximum growth rate of <jats:italic>E. faecalis</jats:italic> FAIR-E 229 and glucose catabolism, although citrate itself was not catabolized. Glucose was converted stoichiometrically into lactate, while small amounts of ethanol were produced as well. Finally, when increasing initial concentrations of citrate (10, 40, 70, and 100 mM) were used as the sole carbon sources in MRS broth without glucose, the main end products were acetate and formate. Small amounts of lactate, ethanol, and acetoin were also detected. This work strongly supports the suggestion that enterococcal strains have the metabolic potential to metabolize citrate and therefore to actively contribute to the flavor development of fermented dairy products. </jats:p> Citrate Metabolism by <i>Enterococcus faecalis</i> FAIR-E 229 Applied and Environmental Microbiology |
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| title |
Citrate Metabolism by Enterococcus faecalis FAIR-E 229 |
| title_unstemmed |
Citrate Metabolism by Enterococcus faecalis FAIR-E 229 |
| title_full |
Citrate Metabolism by Enterococcus faecalis FAIR-E 229 |
| title_fullStr |
Citrate Metabolism by Enterococcus faecalis FAIR-E 229 |
| title_full_unstemmed |
Citrate Metabolism by Enterococcus faecalis FAIR-E 229 |
| title_short |
Citrate Metabolism by Enterococcus faecalis FAIR-E 229 |
| title_sort |
citrate metabolism by
<i>enterococcus faecalis</i>
fair-e 229 |
| topic |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
| url |
http://dx.doi.org/10.1128/aem.67.12.5482-5487.2001 |
| publishDate |
2001 |
| physical |
5482-5487 |
| description |
<jats:title>ABSTRACT</jats:title>
<jats:p>
Citrate metabolism by
<jats:italic>Enterococcus faecalis</jats:italic>
FAIR-E 229 was studied in various growth media containing citrate either in the presence of glucose or lactose or as the sole carbon source. In skim milk (130 mM lactose, 8 mM citrate), cometabolism of citrate and lactose was observed from the first stages of the growth phase. Lactose was stoichiometrically converted into lactate, while citrate was converted into acetate, formate, and ethanol. When de Man-Rogosa-Sharpe (MRS) broth containing lactose (28 mM) instead of glucose was used,
<jats:italic>E. faecalis</jats:italic>
FAIR-E 229 catabolized only the carbohydrate. Lactate was the major end product, and small amounts of ethanol were also detected. Increasing concentrations of citrate (10, 40, 70, and 100 mM) added to MRS broth enhanced both the maximum growth rate of
<jats:italic>E. faecalis</jats:italic>
FAIR-E 229 and glucose catabolism, although citrate itself was not catabolized. Glucose was converted stoichiometrically into lactate, while small amounts of ethanol were produced as well. Finally, when increasing initial concentrations of citrate (10, 40, 70, and 100 mM) were used as the sole carbon sources in MRS broth without glucose, the main end products were acetate and formate. Small amounts of lactate, ethanol, and acetoin were also detected. This work strongly supports the suggestion that enterococcal strains have the metabolic potential to metabolize citrate and therefore to actively contribute to the flavor development of fermented dairy products.
</jats:p> |
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| author | Sarantinopoulos, Panagiotis, Kalantzopoulos, George, Tsakalidou, Effie |
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| description | <jats:title>ABSTRACT</jats:title> <jats:p> Citrate metabolism by <jats:italic>Enterococcus faecalis</jats:italic> FAIR-E 229 was studied in various growth media containing citrate either in the presence of glucose or lactose or as the sole carbon source. In skim milk (130 mM lactose, 8 mM citrate), cometabolism of citrate and lactose was observed from the first stages of the growth phase. Lactose was stoichiometrically converted into lactate, while citrate was converted into acetate, formate, and ethanol. When de Man-Rogosa-Sharpe (MRS) broth containing lactose (28 mM) instead of glucose was used, <jats:italic>E. faecalis</jats:italic> FAIR-E 229 catabolized only the carbohydrate. Lactate was the major end product, and small amounts of ethanol were also detected. Increasing concentrations of citrate (10, 40, 70, and 100 mM) added to MRS broth enhanced both the maximum growth rate of <jats:italic>E. faecalis</jats:italic> FAIR-E 229 and glucose catabolism, although citrate itself was not catabolized. Glucose was converted stoichiometrically into lactate, while small amounts of ethanol were produced as well. Finally, when increasing initial concentrations of citrate (10, 40, 70, and 100 mM) were used as the sole carbon sources in MRS broth without glucose, the main end products were acetate and formate. Small amounts of lactate, ethanol, and acetoin were also detected. This work strongly supports the suggestion that enterococcal strains have the metabolic potential to metabolize citrate and therefore to actively contribute to the flavor development of fermented dairy products. </jats:p> |
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| institution | DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3 |
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| spelling | Sarantinopoulos, Panagiotis Kalantzopoulos, George Tsakalidou, Effie 0099-2240 1098-5336 American Society for Microbiology Ecology Applied Microbiology and Biotechnology Food Science Biotechnology http://dx.doi.org/10.1128/aem.67.12.5482-5487.2001 <jats:title>ABSTRACT</jats:title> <jats:p> Citrate metabolism by <jats:italic>Enterococcus faecalis</jats:italic> FAIR-E 229 was studied in various growth media containing citrate either in the presence of glucose or lactose or as the sole carbon source. In skim milk (130 mM lactose, 8 mM citrate), cometabolism of citrate and lactose was observed from the first stages of the growth phase. Lactose was stoichiometrically converted into lactate, while citrate was converted into acetate, formate, and ethanol. When de Man-Rogosa-Sharpe (MRS) broth containing lactose (28 mM) instead of glucose was used, <jats:italic>E. faecalis</jats:italic> FAIR-E 229 catabolized only the carbohydrate. Lactate was the major end product, and small amounts of ethanol were also detected. Increasing concentrations of citrate (10, 40, 70, and 100 mM) added to MRS broth enhanced both the maximum growth rate of <jats:italic>E. faecalis</jats:italic> FAIR-E 229 and glucose catabolism, although citrate itself was not catabolized. Glucose was converted stoichiometrically into lactate, while small amounts of ethanol were produced as well. Finally, when increasing initial concentrations of citrate (10, 40, 70, and 100 mM) were used as the sole carbon sources in MRS broth without glucose, the main end products were acetate and formate. Small amounts of lactate, ethanol, and acetoin were also detected. This work strongly supports the suggestion that enterococcal strains have the metabolic potential to metabolize citrate and therefore to actively contribute to the flavor development of fermented dairy products. </jats:p> Citrate Metabolism by <i>Enterococcus faecalis</i> FAIR-E 229 Applied and Environmental Microbiology |
| spellingShingle | Sarantinopoulos, Panagiotis, Kalantzopoulos, George, Tsakalidou, Effie, Applied and Environmental Microbiology, Citrate Metabolism by Enterococcus faecalis FAIR-E 229, Ecology, Applied Microbiology and Biotechnology, Food Science, Biotechnology |
| title | Citrate Metabolism by Enterococcus faecalis FAIR-E 229 |
| title_full | Citrate Metabolism by Enterococcus faecalis FAIR-E 229 |
| title_fullStr | Citrate Metabolism by Enterococcus faecalis FAIR-E 229 |
| title_full_unstemmed | Citrate Metabolism by Enterococcus faecalis FAIR-E 229 |
| title_short | Citrate Metabolism by Enterococcus faecalis FAIR-E 229 |
| title_sort | citrate metabolism by <i>enterococcus faecalis</i> fair-e 229 |
| title_unstemmed | Citrate Metabolism by Enterococcus faecalis FAIR-E 229 |
| topic | Ecology, Applied Microbiology and Biotechnology, Food Science, Biotechnology |
| url | http://dx.doi.org/10.1128/aem.67.12.5482-5487.2001 |