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Enumeration of viable bacteria in the marine pelagic environment
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| Zeitschriftentitel: | Applied and Environmental Microbiology |
|---|---|
| Personen und Körperschaften: | , |
| In: | Applied and Environmental Microbiology, 62, 1996, 1, S. 174-177 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Bianchi, A Giuliano, L Bianchi, A Giuliano, L |
|---|---|
| author |
Bianchi, A Giuliano, L |
| spellingShingle |
Bianchi, A Giuliano, L Applied and Environmental Microbiology Enumeration of viable bacteria in the marine pelagic environment Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
| author_sort |
bianchi, a |
| spelling |
Bianchi, A Giuliano, L 0099-2240 1098-5336 American Society for Microbiology Ecology Applied Microbiology and Biotechnology Food Science Biotechnology http://dx.doi.org/10.1128/aem.62.1.174-177.1996 <jats:p>The low percentage of living bacteria commonly obtained when comparing viable counts with total direct counts in seawater could be due more to inappropriate techniques for appreciating the growth ability of living cells than to unadapted culture conditions. The most-probable-number counts in filtered seawater cultures and the microscopic counts of 4(prm1),6-diamidino-2-phenylindole (DAPI)-stained aggregate-forming units grown on black polycarbonate filters appeared significantly correlated to the direct counts. Both these techniques show that in the superficial and intermediate water masses, the living cells may constitute an important (frequently higher than 20%) but highly variable part of the total populations. These viable counts appear more realistic than the conventional CFU counts, which provide only 0.001 to 0.2% of the total counts.</jats:p> Enumeration of viable bacteria in the marine pelagic environment Applied and Environmental Microbiology |
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American Society for Microbiology, 1996 |
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Applied and Environmental Microbiology |
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| title |
Enumeration of viable bacteria in the marine pelagic environment |
| title_unstemmed |
Enumeration of viable bacteria in the marine pelagic environment |
| title_full |
Enumeration of viable bacteria in the marine pelagic environment |
| title_fullStr |
Enumeration of viable bacteria in the marine pelagic environment |
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Enumeration of viable bacteria in the marine pelagic environment |
| title_short |
Enumeration of viable bacteria in the marine pelagic environment |
| title_sort |
enumeration of viable bacteria in the marine pelagic environment |
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Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
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http://dx.doi.org/10.1128/aem.62.1.174-177.1996 |
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1996 |
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174-177 |
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<jats:p>The low percentage of living bacteria commonly obtained when comparing viable counts with total direct counts in seawater could be due more to inappropriate techniques for appreciating the growth ability of living cells than to unadapted culture conditions. The most-probable-number counts in filtered seawater cultures and the microscopic counts of 4(prm1),6-diamidino-2-phenylindole (DAPI)-stained aggregate-forming units grown on black polycarbonate filters appeared significantly correlated to the direct counts. Both these techniques show that in the superficial and intermediate water masses, the living cells may constitute an important (frequently higher than 20%) but highly variable part of the total populations. These viable counts appear more realistic than the conventional CFU counts, which provide only 0.001 to 0.2% of the total counts.</jats:p> |
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| description | <jats:p>The low percentage of living bacteria commonly obtained when comparing viable counts with total direct counts in seawater could be due more to inappropriate techniques for appreciating the growth ability of living cells than to unadapted culture conditions. The most-probable-number counts in filtered seawater cultures and the microscopic counts of 4(prm1),6-diamidino-2-phenylindole (DAPI)-stained aggregate-forming units grown on black polycarbonate filters appeared significantly correlated to the direct counts. Both these techniques show that in the superficial and intermediate water masses, the living cells may constitute an important (frequently higher than 20%) but highly variable part of the total populations. These viable counts appear more realistic than the conventional CFU counts, which provide only 0.001 to 0.2% of the total counts.</jats:p> |
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| spelling | Bianchi, A Giuliano, L 0099-2240 1098-5336 American Society for Microbiology Ecology Applied Microbiology and Biotechnology Food Science Biotechnology http://dx.doi.org/10.1128/aem.62.1.174-177.1996 <jats:p>The low percentage of living bacteria commonly obtained when comparing viable counts with total direct counts in seawater could be due more to inappropriate techniques for appreciating the growth ability of living cells than to unadapted culture conditions. The most-probable-number counts in filtered seawater cultures and the microscopic counts of 4(prm1),6-diamidino-2-phenylindole (DAPI)-stained aggregate-forming units grown on black polycarbonate filters appeared significantly correlated to the direct counts. Both these techniques show that in the superficial and intermediate water masses, the living cells may constitute an important (frequently higher than 20%) but highly variable part of the total populations. These viable counts appear more realistic than the conventional CFU counts, which provide only 0.001 to 0.2% of the total counts.</jats:p> Enumeration of viable bacteria in the marine pelagic environment Applied and Environmental Microbiology |
| spellingShingle | Bianchi, A, Giuliano, L, Applied and Environmental Microbiology, Enumeration of viable bacteria in the marine pelagic environment, Ecology, Applied Microbiology and Biotechnology, Food Science, Biotechnology |
| title | Enumeration of viable bacteria in the marine pelagic environment |
| title_full | Enumeration of viable bacteria in the marine pelagic environment |
| title_fullStr | Enumeration of viable bacteria in the marine pelagic environment |
| title_full_unstemmed | Enumeration of viable bacteria in the marine pelagic environment |
| title_short | Enumeration of viable bacteria in the marine pelagic environment |
| title_sort | enumeration of viable bacteria in the marine pelagic environment |
| title_unstemmed | Enumeration of viable bacteria in the marine pelagic environment |
| topic | Ecology, Applied Microbiology and Biotechnology, Food Science, Biotechnology |
| url | http://dx.doi.org/10.1128/aem.62.1.174-177.1996 |