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Feeding of Enterococcus faecium NCIMB 10415 Leads to Intestinal miRNA-423-5p-Induced Regulation of Immune-Relevant Genes

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Bibliographische Detailangaben
Zeitschriftentitel: Applied and Environmental Microbiology
Personen und Körperschaften: Kreuzer-Redmer, Susanne, Bekurtz, Jennifer C., Arends, Danny, Bortfeldt, Ralf, Kutz-Lohroff, Barbara, Sharbati, Soroush, Einspanier, Ralf, Brockmann, Gudrun A.
In: Applied and Environmental Microbiology, 82, 2016, 8, S. 2263-2269
Format: E-Article
Sprache: Englisch
veröffentlicht:
American Society for Microbiology
Schlagwörter:
Ecology
Applied Microbiology and Biotechnology
Food Science
Biotechnology
Details
Zusammenfassung: <jats:title>ABSTRACT</jats:title> <jats:p> Probiotics are widely used in human and animal health, but little is known about the mode of action of probiotics. One possible mechanism at the molecular level could be an influence on microRNAs (miRNAs) and the related immune-relevant target genes. Here, we analyzed differential expression of miRNA and potential target genes of ileal and jejunal lymphatic tissues from <jats:named-content content-type="genus-species">Enterococcus faecium</jats:named-content> NCIMB 10415-fed piglets versus untreated controls by using next-generation sequencing. We identified miR-423-5p as being greatly affected by the treatment group (2.32-fold; <jats:italic>P</jats:italic> = 0.014). Validation by reverse transcription-quantitative PCR (RT-qPCR) confirmed a significant upregulation of miR-423-5p (2.11-fold; <jats:italic>P</jats:italic> = 0.03) and, additionally, downregulation of the important immune-relevant immunoglobulin lambda light C region (IGLC) (0.61-fold; <jats:italic>P</jats:italic> = 0.03) and immunoglobulin kappa constant (IGKC) (0.69-fold; <jats:italic>P</jats:italic> = 0.04) target genes. Expression analysis of miR-423-5p and IGLC at different age points shows a clear anticorrelated relationship. Luciferase reporter assays with a HeLa cell line verified IGLC as a target of miR-423-5p. The results provided evidence for an effect of feeding of <jats:named-content content-type="genus-species">E. faecium</jats:named-content> on the expression of miR-423-5p and on the regulation of the IGLC gene through miR-423-5p. This might be a possible mode of action of <jats:named-content content-type="genus-species">E. faecium</jats:named-content> on immune cell regulation in the small intestine. </jats:p>
Umfang: 2263-2269
ISSN: 0099-2240
1098-5336
DOI: 10.1128/aem.04044-15