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Role of Malic Enzyme during Fatty Acid Synthesis in the Oleaginous Fungus Mortierella alpina
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| Zeitschriftentitel: | Applied and Environmental Microbiology |
|---|---|
| Personen und Körperschaften: | , , , , , , , |
| In: | Applied and Environmental Microbiology, 80, 2014, 9, S. 2672-2678 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
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| Schlagwörter: |
| author_facet |
Hao, Guangfei Chen, Haiqin Wang, Lei Gu, Zhennan Song, Yuanda Zhang, Hao Chen, Wei Chen, Yong Q. Hao, Guangfei Chen, Haiqin Wang, Lei Gu, Zhennan Song, Yuanda Zhang, Hao Chen, Wei Chen, Yong Q. |
|---|---|
| author |
Hao, Guangfei Chen, Haiqin Wang, Lei Gu, Zhennan Song, Yuanda Zhang, Hao Chen, Wei Chen, Yong Q. |
| spellingShingle |
Hao, Guangfei Chen, Haiqin Wang, Lei Gu, Zhennan Song, Yuanda Zhang, Hao Chen, Wei Chen, Yong Q. Applied and Environmental Microbiology Role of Malic Enzyme during Fatty Acid Synthesis in the Oleaginous Fungus Mortierella alpina Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
| author_sort |
hao, guangfei |
| spelling |
Hao, Guangfei Chen, Haiqin Wang, Lei Gu, Zhennan Song, Yuanda Zhang, Hao Chen, Wei Chen, Yong Q. 0099-2240 1098-5336 American Society for Microbiology Ecology Applied Microbiology and Biotechnology Food Science Biotechnology http://dx.doi.org/10.1128/aem.00140-14 <jats:title>ABSTRACT</jats:title> <jats:p> The generation of NADPH by malic enzyme (ME) was postulated to be a rate-limiting step during fatty acid synthesis in oleaginous fungi, based primarily on the results from research focusing on ME in <jats:named-content content-type="genus-species">Mucor circinelloides</jats:named-content> . This hypothesis is challenged by a recent study showing that leucine metabolism, rather than ME, is critical for fatty acid synthesis in <jats:named-content content-type="genus-species">M. circinelloides</jats:named-content> . To clarify this, the gene encoding ME isoform E from <jats:named-content content-type="genus-species">Mortierella alpina</jats:named-content> was homologously expressed. ME overexpression increased the fatty acid content by 30% compared to that for a control. Our results suggest that ME may not be the sole rate-limiting enzyme, but does play a role, during fatty acid synthesis in oleaginous fungi. </jats:p> Role of Malic Enzyme during Fatty Acid Synthesis in the Oleaginous Fungus Mortierella alpina Applied and Environmental Microbiology |
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10.1128/aem.00140-14 |
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| title |
Role of Malic Enzyme during Fatty Acid Synthesis in the Oleaginous Fungus Mortierella alpina |
| title_unstemmed |
Role of Malic Enzyme during Fatty Acid Synthesis in the Oleaginous Fungus Mortierella alpina |
| title_full |
Role of Malic Enzyme during Fatty Acid Synthesis in the Oleaginous Fungus Mortierella alpina |
| title_fullStr |
Role of Malic Enzyme during Fatty Acid Synthesis in the Oleaginous Fungus Mortierella alpina |
| title_full_unstemmed |
Role of Malic Enzyme during Fatty Acid Synthesis in the Oleaginous Fungus Mortierella alpina |
| title_short |
Role of Malic Enzyme during Fatty Acid Synthesis in the Oleaginous Fungus Mortierella alpina |
| title_sort |
role of malic enzyme during fatty acid synthesis in the oleaginous fungus mortierella alpina |
| topic |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
| url |
http://dx.doi.org/10.1128/aem.00140-14 |
| publishDate |
2014 |
| physical |
2672-2678 |
| description |
<jats:title>ABSTRACT</jats:title>
<jats:p>
The generation of NADPH by malic enzyme (ME) was postulated to be a rate-limiting step during fatty acid synthesis in oleaginous fungi, based primarily on the results from research focusing on ME in
<jats:named-content content-type="genus-species">Mucor circinelloides</jats:named-content>
. This hypothesis is challenged by a recent study showing that leucine metabolism, rather than ME, is critical for fatty acid synthesis in
<jats:named-content content-type="genus-species">M. circinelloides</jats:named-content>
. To clarify this, the gene encoding ME isoform E from
<jats:named-content content-type="genus-species">Mortierella alpina</jats:named-content>
was homologously expressed. ME overexpression increased the fatty acid content by 30% compared to that for a control. Our results suggest that ME may not be the sole rate-limiting enzyme, but does play a role, during fatty acid synthesis in oleaginous fungi.
</jats:p> |
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| author | Hao, Guangfei, Chen, Haiqin, Wang, Lei, Gu, Zhennan, Song, Yuanda, Zhang, Hao, Chen, Wei, Chen, Yong Q. |
| author_facet | Hao, Guangfei, Chen, Haiqin, Wang, Lei, Gu, Zhennan, Song, Yuanda, Zhang, Hao, Chen, Wei, Chen, Yong Q., Hao, Guangfei, Chen, Haiqin, Wang, Lei, Gu, Zhennan, Song, Yuanda, Zhang, Hao, Chen, Wei, Chen, Yong Q. |
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| description | <jats:title>ABSTRACT</jats:title> <jats:p> The generation of NADPH by malic enzyme (ME) was postulated to be a rate-limiting step during fatty acid synthesis in oleaginous fungi, based primarily on the results from research focusing on ME in <jats:named-content content-type="genus-species">Mucor circinelloides</jats:named-content> . This hypothesis is challenged by a recent study showing that leucine metabolism, rather than ME, is critical for fatty acid synthesis in <jats:named-content content-type="genus-species">M. circinelloides</jats:named-content> . To clarify this, the gene encoding ME isoform E from <jats:named-content content-type="genus-species">Mortierella alpina</jats:named-content> was homologously expressed. ME overexpression increased the fatty acid content by 30% compared to that for a control. Our results suggest that ME may not be the sole rate-limiting enzyme, but does play a role, during fatty acid synthesis in oleaginous fungi. </jats:p> |
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| physical | 2672-2678 |
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| spelling | Hao, Guangfei Chen, Haiqin Wang, Lei Gu, Zhennan Song, Yuanda Zhang, Hao Chen, Wei Chen, Yong Q. 0099-2240 1098-5336 American Society for Microbiology Ecology Applied Microbiology and Biotechnology Food Science Biotechnology http://dx.doi.org/10.1128/aem.00140-14 <jats:title>ABSTRACT</jats:title> <jats:p> The generation of NADPH by malic enzyme (ME) was postulated to be a rate-limiting step during fatty acid synthesis in oleaginous fungi, based primarily on the results from research focusing on ME in <jats:named-content content-type="genus-species">Mucor circinelloides</jats:named-content> . This hypothesis is challenged by a recent study showing that leucine metabolism, rather than ME, is critical for fatty acid synthesis in <jats:named-content content-type="genus-species">M. circinelloides</jats:named-content> . To clarify this, the gene encoding ME isoform E from <jats:named-content content-type="genus-species">Mortierella alpina</jats:named-content> was homologously expressed. ME overexpression increased the fatty acid content by 30% compared to that for a control. Our results suggest that ME may not be the sole rate-limiting enzyme, but does play a role, during fatty acid synthesis in oleaginous fungi. </jats:p> Role of Malic Enzyme during Fatty Acid Synthesis in the Oleaginous Fungus Mortierella alpina Applied and Environmental Microbiology |
| spellingShingle | Hao, Guangfei, Chen, Haiqin, Wang, Lei, Gu, Zhennan, Song, Yuanda, Zhang, Hao, Chen, Wei, Chen, Yong Q., Applied and Environmental Microbiology, Role of Malic Enzyme during Fatty Acid Synthesis in the Oleaginous Fungus Mortierella alpina, Ecology, Applied Microbiology and Biotechnology, Food Science, Biotechnology |
| title | Role of Malic Enzyme during Fatty Acid Synthesis in the Oleaginous Fungus Mortierella alpina |
| title_full | Role of Malic Enzyme during Fatty Acid Synthesis in the Oleaginous Fungus Mortierella alpina |
| title_fullStr | Role of Malic Enzyme during Fatty Acid Synthesis in the Oleaginous Fungus Mortierella alpina |
| title_full_unstemmed | Role of Malic Enzyme during Fatty Acid Synthesis in the Oleaginous Fungus Mortierella alpina |
| title_short | Role of Malic Enzyme during Fatty Acid Synthesis in the Oleaginous Fungus Mortierella alpina |
| title_sort | role of malic enzyme during fatty acid synthesis in the oleaginous fungus mortierella alpina |
| title_unstemmed | Role of Malic Enzyme during Fatty Acid Synthesis in the Oleaginous Fungus Mortierella alpina |
| topic | Ecology, Applied Microbiology and Biotechnology, Food Science, Biotechnology |
| url | http://dx.doi.org/10.1128/aem.00140-14 |