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Increased Survival of Antibiotic-Resistant Escherichia coli inside Macrophages
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| Zeitschriftentitel: | Antimicrobial Agents and Chemotherapy |
|---|---|
| Personen und Körperschaften: | , |
| In: | Antimicrobial Agents and Chemotherapy, 57, 2013, 1, S. 189-195 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Miskinyte, Migla Gordo, Isabel Miskinyte, Migla Gordo, Isabel |
|---|---|
| author |
Miskinyte, Migla Gordo, Isabel |
| spellingShingle |
Miskinyte, Migla Gordo, Isabel Antimicrobial Agents and Chemotherapy Increased Survival of Antibiotic-Resistant Escherichia coli inside Macrophages Infectious Diseases Pharmacology (medical) Pharmacology |
| author_sort |
miskinyte, migla |
| spelling |
Miskinyte, Migla Gordo, Isabel 0066-4804 1098-6596 American Society for Microbiology Infectious Diseases Pharmacology (medical) Pharmacology http://dx.doi.org/10.1128/aac.01632-12 <jats:title>ABSTRACT</jats:title> <jats:p> Mutations causing antibiotic resistance usually incur a fitness cost in the absence of antibiotics. The magnitude of such costs is known to vary with the environment. Little is known about the fitness effects of antibiotic resistance mutations when bacteria confront the host's immune system. Here, we study the fitness effects of mutations in the <jats:italic>rpoB</jats:italic> , <jats:italic>rpsL</jats:italic> , and <jats:italic>gyrA</jats:italic> genes, which confer resistance to rifampin, streptomycin, and nalidixic acid, respectively. These antibiotics are frequently used in the treatment of bacterial infections. We measured two important fitness traits—growth rate and survival ability—of 12 <jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="genus-species" xlink:type="simple">Escherichia coli</jats:named-content> K-12 strains, each carrying a single resistance mutation, in the presence of macrophages. Strikingly, we found that 67% of the mutants survived better than the susceptible bacteria in the intracellular niche of the phagocytic cells. In particular, all <jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="genus-species" xlink:type="simple">E. coli</jats:named-content> streptomycin-resistant mutants exhibited an intracellular advantage. On the other hand, 42% of the mutants incurred a high fitness cost when the bacteria were allowed to divide outside of macrophages. This study shows that single nonsynonymous changes affecting fundamental processes in the cell can contribute to prolonged survival of <jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="genus-species" xlink:type="simple">E. coli</jats:named-content> in the context of an infection. </jats:p> Increased Survival of Antibiotic-Resistant Escherichia coli inside Macrophages Antimicrobial Agents and Chemotherapy |
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10.1128/aac.01632-12 |
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American Society for Microbiology, 2013 |
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American Society for Microbiology, 2013 |
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0066-4804 1098-6596 |
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Antimicrobial Agents and Chemotherapy |
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49 |
| title |
Increased Survival of Antibiotic-Resistant Escherichia coli inside Macrophages |
| title_unstemmed |
Increased Survival of Antibiotic-Resistant Escherichia coli inside Macrophages |
| title_full |
Increased Survival of Antibiotic-Resistant Escherichia coli inside Macrophages |
| title_fullStr |
Increased Survival of Antibiotic-Resistant Escherichia coli inside Macrophages |
| title_full_unstemmed |
Increased Survival of Antibiotic-Resistant Escherichia coli inside Macrophages |
| title_short |
Increased Survival of Antibiotic-Resistant Escherichia coli inside Macrophages |
| title_sort |
increased survival of antibiotic-resistant escherichia coli inside macrophages |
| topic |
Infectious Diseases Pharmacology (medical) Pharmacology |
| url |
http://dx.doi.org/10.1128/aac.01632-12 |
| publishDate |
2013 |
| physical |
189-195 |
| description |
<jats:title>ABSTRACT</jats:title>
<jats:p>
Mutations causing antibiotic resistance usually incur a fitness cost in the absence of antibiotics. The magnitude of such costs is known to vary with the environment. Little is known about the fitness effects of antibiotic resistance mutations when bacteria confront the host's immune system. Here, we study the fitness effects of mutations in the
<jats:italic>rpoB</jats:italic>
,
<jats:italic>rpsL</jats:italic>
, and
<jats:italic>gyrA</jats:italic>
genes, which confer resistance to rifampin, streptomycin, and nalidixic acid, respectively. These antibiotics are frequently used in the treatment of bacterial infections. We measured two important fitness traits—growth rate and survival ability—of 12
<jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="genus-species" xlink:type="simple">Escherichia coli</jats:named-content>
K-12 strains, each carrying a single resistance mutation, in the presence of macrophages. Strikingly, we found that 67% of the mutants survived better than the susceptible bacteria in the intracellular niche of the phagocytic cells. In particular, all
<jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="genus-species" xlink:type="simple">E. coli</jats:named-content>
streptomycin-resistant mutants exhibited an intracellular advantage. On the other hand, 42% of the mutants incurred a high fitness cost when the bacteria were allowed to divide outside of macrophages. This study shows that single nonsynonymous changes affecting fundamental processes in the cell can contribute to prolonged survival of
<jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="genus-species" xlink:type="simple">E. coli</jats:named-content>
in the context of an infection.
</jats:p> |
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| author | Miskinyte, Migla, Gordo, Isabel |
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| description | <jats:title>ABSTRACT</jats:title> <jats:p> Mutations causing antibiotic resistance usually incur a fitness cost in the absence of antibiotics. The magnitude of such costs is known to vary with the environment. Little is known about the fitness effects of antibiotic resistance mutations when bacteria confront the host's immune system. Here, we study the fitness effects of mutations in the <jats:italic>rpoB</jats:italic> , <jats:italic>rpsL</jats:italic> , and <jats:italic>gyrA</jats:italic> genes, which confer resistance to rifampin, streptomycin, and nalidixic acid, respectively. These antibiotics are frequently used in the treatment of bacterial infections. We measured two important fitness traits—growth rate and survival ability—of 12 <jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="genus-species" xlink:type="simple">Escherichia coli</jats:named-content> K-12 strains, each carrying a single resistance mutation, in the presence of macrophages. Strikingly, we found that 67% of the mutants survived better than the susceptible bacteria in the intracellular niche of the phagocytic cells. In particular, all <jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="genus-species" xlink:type="simple">E. coli</jats:named-content> streptomycin-resistant mutants exhibited an intracellular advantage. On the other hand, 42% of the mutants incurred a high fitness cost when the bacteria were allowed to divide outside of macrophages. This study shows that single nonsynonymous changes affecting fundamental processes in the cell can contribute to prolonged survival of <jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="genus-species" xlink:type="simple">E. coli</jats:named-content> in the context of an infection. </jats:p> |
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| spelling | Miskinyte, Migla Gordo, Isabel 0066-4804 1098-6596 American Society for Microbiology Infectious Diseases Pharmacology (medical) Pharmacology http://dx.doi.org/10.1128/aac.01632-12 <jats:title>ABSTRACT</jats:title> <jats:p> Mutations causing antibiotic resistance usually incur a fitness cost in the absence of antibiotics. The magnitude of such costs is known to vary with the environment. Little is known about the fitness effects of antibiotic resistance mutations when bacteria confront the host's immune system. Here, we study the fitness effects of mutations in the <jats:italic>rpoB</jats:italic> , <jats:italic>rpsL</jats:italic> , and <jats:italic>gyrA</jats:italic> genes, which confer resistance to rifampin, streptomycin, and nalidixic acid, respectively. These antibiotics are frequently used in the treatment of bacterial infections. We measured two important fitness traits—growth rate and survival ability—of 12 <jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="genus-species" xlink:type="simple">Escherichia coli</jats:named-content> K-12 strains, each carrying a single resistance mutation, in the presence of macrophages. Strikingly, we found that 67% of the mutants survived better than the susceptible bacteria in the intracellular niche of the phagocytic cells. In particular, all <jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="genus-species" xlink:type="simple">E. coli</jats:named-content> streptomycin-resistant mutants exhibited an intracellular advantage. On the other hand, 42% of the mutants incurred a high fitness cost when the bacteria were allowed to divide outside of macrophages. This study shows that single nonsynonymous changes affecting fundamental processes in the cell can contribute to prolonged survival of <jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="genus-species" xlink:type="simple">E. coli</jats:named-content> in the context of an infection. </jats:p> Increased Survival of Antibiotic-Resistant Escherichia coli inside Macrophages Antimicrobial Agents and Chemotherapy |
| spellingShingle | Miskinyte, Migla, Gordo, Isabel, Antimicrobial Agents and Chemotherapy, Increased Survival of Antibiotic-Resistant Escherichia coli inside Macrophages, Infectious Diseases, Pharmacology (medical), Pharmacology |
| title | Increased Survival of Antibiotic-Resistant Escherichia coli inside Macrophages |
| title_full | Increased Survival of Antibiotic-Resistant Escherichia coli inside Macrophages |
| title_fullStr | Increased Survival of Antibiotic-Resistant Escherichia coli inside Macrophages |
| title_full_unstemmed | Increased Survival of Antibiotic-Resistant Escherichia coli inside Macrophages |
| title_short | Increased Survival of Antibiotic-Resistant Escherichia coli inside Macrophages |
| title_sort | increased survival of antibiotic-resistant escherichia coli inside macrophages |
| title_unstemmed | Increased Survival of Antibiotic-Resistant Escherichia coli inside Macrophages |
| topic | Infectious Diseases, Pharmacology (medical), Pharmacology |
| url | http://dx.doi.org/10.1128/aac.01632-12 |