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Ectopic expression of ORANGE promotes carotenoid accumulation and fruit development in tomato

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Bibliographische Detailangaben
Zeitschriftentitel: Plant Biotechnology Journal
Personen und Körperschaften: Yazdani, Mohammad, Sun, Zhaoxia, Yuan, Hui, Zeng, Shaohua, Thannhauser, Theodore W., Vrebalov, Julia, Ma, Qiyue, Xu, Yimin, Fei, Zhangjun, Van Eck, Joyce, Tian, Shiping, Tadmor, Yaakov, Giovannoni, James J., Li, Li
In: Plant Biotechnology Journal, 17, 2019, 1, S. 33-49
Format: E-Article
Sprache: Englisch
veröffentlicht:
Wiley
Schlagwörter:
Plant Science
Agronomy and Crop Science
Biotechnology
Details
Zusammenfassung: <jats:title>Summary</jats:title><jats:p>Carotenoids are critically important to plants and humans. The <jats:italic><jats:styled-content style="fixed-case">ORANGE</jats:styled-content></jats:italic> (<jats:italic><jats:styled-content style="fixed-case">OR</jats:styled-content></jats:italic>) gene is a key regulator for carotenoid accumulation, but its physiological roles in crops remain elusive. In this study, we generated transgenic tomato ectopically overexpressing the Arabidopsis wild‐type <jats:italic><jats:styled-content style="fixed-case">OR</jats:styled-content></jats:italic> (<jats:italic>At</jats:italic><jats:styled-content style="fixed-case"><jats:italic>OR</jats:italic><jats:sup><jats:italic>WT</jats:italic></jats:sup></jats:styled-content>) and a ‘golden <jats:styled-content style="fixed-case">SNP</jats:styled-content>’‐containing <jats:italic><jats:styled-content style="fixed-case">OR</jats:styled-content></jats:italic> (<jats:italic>At</jats:italic><jats:styled-content style="fixed-case"><jats:italic>OR</jats:italic><jats:sup><jats:italic>H</jats:italic></jats:sup></jats:styled-content><jats:sup><jats:italic>is</jats:italic></jats:sup>). We found that <jats:italic>At</jats:italic><jats:styled-content style="fixed-case"><jats:italic>OR</jats:italic><jats:sup><jats:italic>H</jats:italic></jats:sup></jats:styled-content><jats:sup><jats:italic>is</jats:italic></jats:sup> initiated chromoplast formation in very young fruit and stimulated carotenoid accumulation at all fruit developmental stages, uncoupled from other ripening activities. The elevated levels of carotenoids in the <jats:italic>At<jats:styled-content style="fixed-case">OR</jats:styled-content></jats:italic> lines were distributed in the same subplastidial fractions as in wild‐type tomato, indicating an adaptive response of plastids to sequester the increased carotenoids. Microscopic analysis revealed that the plastid sizes were increased in both <jats:italic>At</jats:italic><jats:styled-content style="fixed-case"><jats:italic>OR</jats:italic><jats:sup><jats:italic>WT</jats:italic></jats:sup></jats:styled-content> and <jats:italic>At</jats:italic><jats:styled-content style="fixed-case"><jats:italic>OR</jats:italic><jats:sup><jats:italic>H</jats:italic></jats:sup></jats:styled-content><jats:sup><jats:italic>is</jats:italic></jats:sup> lines at early fruit developmental stages. Moreover, <jats:italic>At<jats:styled-content style="fixed-case">OR</jats:styled-content></jats:italic> overexpression promoted early flowering, fruit set and seed production. Ethylene production and the expression of ripening‐associated genes were also significantly increased in the <jats:italic>At<jats:styled-content style="fixed-case">OR</jats:styled-content></jats:italic> transgenic fruit at ripening stages. <jats:styled-content style="fixed-case">RNA</jats:styled-content>‐Seq transcriptomic profiling highlighted the primary effects of <jats:italic><jats:styled-content style="fixed-case">OR</jats:styled-content></jats:italic> overexpression on the genes in the processes related to <jats:styled-content style="fixed-case">RNA</jats:styled-content>, protein and signalling in tomato fruit. Taken together, these results expand our understanding of <jats:italic><jats:styled-content style="fixed-case">OR</jats:styled-content></jats:italic> in mediating carotenoid accumulation in plants and suggest additional roles of <jats:italic><jats:styled-content style="fixed-case">OR</jats:styled-content></jats:italic> in affecting plastid size as well as flower and fruit development, thus making <jats:italic><jats:styled-content style="fixed-case">OR</jats:styled-content></jats:italic> a target gene not only for nutritional biofortification of agricultural products but also for alteration of horticultural traits.</jats:p>
Umfang: 33-49
ISSN: 1467-7644
1467-7652
DOI: 10.1111/pbi.12945