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The role of basophil activation test in special populations with mastocytosis and reactions to hymenoptera sting
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| Zeitschriftentitel: | Allergy |
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| Personen und Körperschaften: | , , , , , , , |
| In: | Allergy, 67, 2012, 7, S. 962-965 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
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| Schlagwörter: |
| Zusammenfassung: | <jats:title>Abstract</jats:title><jats:sec><jats:title>Background</jats:title><jats:p>Systemic mastocytosis (<jats:styled-content style="fixed-case">SM</jats:styled-content>) may be associated with hymenoptera allergy. In such cases, immunotherapy is a life‐saving treatment, but a circumstantiated diagnosis is needed for its prescription. Patients with <jats:styled-content style="fixed-case">SM</jats:styled-content> and previous reactions to stings, but with negative tests represent a diagnostic dilemma. The basophil activation test (<jats:styled-content style="fixed-case">BAT</jats:styled-content>) may be helpful in refining the diagnosis.</jats:p></jats:sec><jats:sec><jats:title>Objective</jats:title><jats:p>We assessed the usefulness of <jats:styled-content style="fixed-case">BAT</jats:styled-content> in subpopulations of mastocytosis patients, including those with negative tests for insect allergy.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>Within a population of patients with mastocytosis and previous stings, we studied by <jats:styled-content style="fixed-case">BAT</jats:styled-content> and augmented intradermal test (<jats:styled-content style="fixed-case">IDT</jats:styled-content>) (10 µg/ml) two groups: (1) with reactions to stings and negative tests; (2) without reactions and negative tests. Basophil activation test was performed with different venoms, assessing at flow cytometry basophils’ activation.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>Sixty‐three patients had mastocytosis and 52 had reactions to previous hymenoptera stings. Of them, seven proved negative to diagnostic tests. In six of seven of those patients, BAT was negative with all venoms, and in one, basophils resulted activated also with the negative control. In six patients without previous reactions and negative tests, BAT was totally negative in five of six patients and weakly positive to Hornet in one. Finally, the <jats:styled-content style="fixed-case">IDT</jats:styled-content> at 10 µg/ml venom produced nonspecific positive results in most cases.</jats:p></jats:sec><jats:sec><jats:title>Conclusion</jats:title><jats:p>In patients with mastocytosis, the negative results of standard tests are reliable, because <jats:styled-content style="fixed-case">BAT</jats:styled-content> and <jats:styled-content style="fixed-case">IDT</jats:styled-content> at higher concentration do not add useful information.</jats:p></jats:sec> |
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| Umfang: | 962-965 |
| ISSN: |
0105-4538
1398-9995 |
| DOI: | 10.1111/j.1398-9995.2012.02849.x |