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Two sources of endogenous hydrogen peroxide in Escherichia coli
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| Zeitschriftentitel: | Molecular Microbiology |
|---|---|
| Personen und Körperschaften: | , |
| In: | Molecular Microbiology, 75, 2010, 6, S. 1389-1401 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
|
| Schlagwörter: |
| author_facet |
Korshunov, Sergei Imlay, James A. Korshunov, Sergei Imlay, James A. |
|---|---|
| author |
Korshunov, Sergei Imlay, James A. |
| spellingShingle |
Korshunov, Sergei Imlay, James A. Molecular Microbiology Two sources of endogenous hydrogen peroxide in Escherichia coli Molecular Biology Microbiology |
| author_sort |
korshunov, sergei |
| spelling |
Korshunov, Sergei Imlay, James A. 0950-382X 1365-2958 Wiley Molecular Biology Microbiology http://dx.doi.org/10.1111/j.1365-2958.2010.07059.x <jats:title>Summary</jats:title><jats:p>Mechanisms of hydrogen peroxide generation in <jats:italic>Escherichia coli</jats:italic> were investigated using a strain lacking scavenging enzymes. Surprisingly, the deletion of many abundant flavoenzymes that are known to autoxidize <jats:italic>in vitro</jats:italic> did not substantially lessen overall H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub> formation. However, H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub> production diminished by 25–30% when NadB turnover was eliminated. The flavin‐dependent desaturating dehydrogenase, NadB uses fumarate as an electron acceptor in anaerobic cells. Experiments showed that aerobic NadB turnover depends upon its oxidation by molecular oxygen, with H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub> as a product. This reaction appears to be mechanistically adventitious. In contrast, most desaturating dehydrogenases are associated with the respiratory chain and deliver electrons to fumarate anaerobically or oxygen aerobically without the formation of toxic by‐products. Presumably, NadB can persist as an H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub>‐generating enzyme because its flux is limited. The anaerobic respiratory enzyme fumarate reductase uses a flavoprotein subunit that is homologous to NadB and accordingly forms substantial H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub> upon aeration. This tendency is substantially suppressed by cytochrome oxidase. Thus cytochrome <jats:italic>d</jats:italic> oxidase, which is prevalent among anaerobes, may diminish intracellular H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub> formation by the anaerobic respiratory chain, whenever these organisms encounter oxygen. These two examples reveal biochemical and physiological arrangements through which evolution has minimized the rate of intracellular oxidant formation.</jats:p> Two sources of endogenous hydrogen peroxide in <i>Escherichia coli</i> Molecular Microbiology |
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| title |
Two sources of endogenous hydrogen peroxide in Escherichia coli |
| title_unstemmed |
Two sources of endogenous hydrogen peroxide in Escherichia coli |
| title_full |
Two sources of endogenous hydrogen peroxide in Escherichia coli |
| title_fullStr |
Two sources of endogenous hydrogen peroxide in Escherichia coli |
| title_full_unstemmed |
Two sources of endogenous hydrogen peroxide in Escherichia coli |
| title_short |
Two sources of endogenous hydrogen peroxide in Escherichia coli |
| title_sort |
two sources of endogenous hydrogen peroxide in <i>escherichia coli</i> |
| topic |
Molecular Biology Microbiology |
| url |
http://dx.doi.org/10.1111/j.1365-2958.2010.07059.x |
| publishDate |
2010 |
| physical |
1389-1401 |
| description |
<jats:title>Summary</jats:title><jats:p>Mechanisms of hydrogen peroxide generation in <jats:italic>Escherichia coli</jats:italic> were investigated using a strain lacking scavenging enzymes. Surprisingly, the deletion of many abundant flavoenzymes that are known to autoxidize <jats:italic>in vitro</jats:italic> did not substantially lessen overall H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub> formation. However, H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub> production diminished by 25–30% when NadB turnover was eliminated. The flavin‐dependent desaturating dehydrogenase, NadB uses fumarate as an electron acceptor in anaerobic cells. Experiments showed that aerobic NadB turnover depends upon its oxidation by molecular oxygen, with H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub> as a product. This reaction appears to be mechanistically adventitious. In contrast, most desaturating dehydrogenases are associated with the respiratory chain and deliver electrons to fumarate anaerobically or oxygen aerobically without the formation of toxic by‐products. Presumably, NadB can persist as an H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub>‐generating enzyme because its flux is limited. The anaerobic respiratory enzyme fumarate reductase uses a flavoprotein subunit that is homologous to NadB and accordingly forms substantial H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub> upon aeration. This tendency is substantially suppressed by cytochrome oxidase. Thus cytochrome <jats:italic>d</jats:italic> oxidase, which is prevalent among anaerobes, may diminish intracellular H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub> formation by the anaerobic respiratory chain, whenever these organisms encounter oxygen. These two examples reveal biochemical and physiological arrangements through which evolution has minimized the rate of intracellular oxidant formation.</jats:p> |
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| author | Korshunov, Sergei, Imlay, James A. |
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| description | <jats:title>Summary</jats:title><jats:p>Mechanisms of hydrogen peroxide generation in <jats:italic>Escherichia coli</jats:italic> were investigated using a strain lacking scavenging enzymes. Surprisingly, the deletion of many abundant flavoenzymes that are known to autoxidize <jats:italic>in vitro</jats:italic> did not substantially lessen overall H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub> formation. However, H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub> production diminished by 25–30% when NadB turnover was eliminated. The flavin‐dependent desaturating dehydrogenase, NadB uses fumarate as an electron acceptor in anaerobic cells. Experiments showed that aerobic NadB turnover depends upon its oxidation by molecular oxygen, with H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub> as a product. This reaction appears to be mechanistically adventitious. In contrast, most desaturating dehydrogenases are associated with the respiratory chain and deliver electrons to fumarate anaerobically or oxygen aerobically without the formation of toxic by‐products. Presumably, NadB can persist as an H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub>‐generating enzyme because its flux is limited. The anaerobic respiratory enzyme fumarate reductase uses a flavoprotein subunit that is homologous to NadB and accordingly forms substantial H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub> upon aeration. This tendency is substantially suppressed by cytochrome oxidase. Thus cytochrome <jats:italic>d</jats:italic> oxidase, which is prevalent among anaerobes, may diminish intracellular H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub> formation by the anaerobic respiratory chain, whenever these organisms encounter oxygen. These two examples reveal biochemical and physiological arrangements through which evolution has minimized the rate of intracellular oxidant formation.</jats:p> |
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| institution | DE-Rs1, DE-Pl11, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15 |
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| spelling | Korshunov, Sergei Imlay, James A. 0950-382X 1365-2958 Wiley Molecular Biology Microbiology http://dx.doi.org/10.1111/j.1365-2958.2010.07059.x <jats:title>Summary</jats:title><jats:p>Mechanisms of hydrogen peroxide generation in <jats:italic>Escherichia coli</jats:italic> were investigated using a strain lacking scavenging enzymes. Surprisingly, the deletion of many abundant flavoenzymes that are known to autoxidize <jats:italic>in vitro</jats:italic> did not substantially lessen overall H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub> formation. However, H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub> production diminished by 25–30% when NadB turnover was eliminated. The flavin‐dependent desaturating dehydrogenase, NadB uses fumarate as an electron acceptor in anaerobic cells. Experiments showed that aerobic NadB turnover depends upon its oxidation by molecular oxygen, with H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub> as a product. This reaction appears to be mechanistically adventitious. In contrast, most desaturating dehydrogenases are associated with the respiratory chain and deliver electrons to fumarate anaerobically or oxygen aerobically without the formation of toxic by‐products. Presumably, NadB can persist as an H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub>‐generating enzyme because its flux is limited. The anaerobic respiratory enzyme fumarate reductase uses a flavoprotein subunit that is homologous to NadB and accordingly forms substantial H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub> upon aeration. This tendency is substantially suppressed by cytochrome oxidase. Thus cytochrome <jats:italic>d</jats:italic> oxidase, which is prevalent among anaerobes, may diminish intracellular H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub> formation by the anaerobic respiratory chain, whenever these organisms encounter oxygen. These two examples reveal biochemical and physiological arrangements through which evolution has minimized the rate of intracellular oxidant formation.</jats:p> Two sources of endogenous hydrogen peroxide in <i>Escherichia coli</i> Molecular Microbiology |
| spellingShingle | Korshunov, Sergei, Imlay, James A., Molecular Microbiology, Two sources of endogenous hydrogen peroxide in Escherichia coli, Molecular Biology, Microbiology |
| title | Two sources of endogenous hydrogen peroxide in Escherichia coli |
| title_full | Two sources of endogenous hydrogen peroxide in Escherichia coli |
| title_fullStr | Two sources of endogenous hydrogen peroxide in Escherichia coli |
| title_full_unstemmed | Two sources of endogenous hydrogen peroxide in Escherichia coli |
| title_short | Two sources of endogenous hydrogen peroxide in Escherichia coli |
| title_sort | two sources of endogenous hydrogen peroxide in <i>escherichia coli</i> |
| title_unstemmed | Two sources of endogenous hydrogen peroxide in Escherichia coli |
| topic | Molecular Biology, Microbiology |
| url | http://dx.doi.org/10.1111/j.1365-2958.2010.07059.x |