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Glycerylphosphorylcholine (GPC) diesterase related alterations in the oxygen consumption profile of rat spermatozoa in differing functional states
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| Zeitschriftentitel: | International Journal of Andrology |
|---|---|
| Personen und Körperschaften: | , |
| In: | International Journal of Andrology, 15, 1992, 4, S. 345-354 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
|
| Schlagwörter: |
| author_facet |
CHOWDHURY, M. MITRA, J. CHOWDHURY, M. MITRA, J. |
|---|---|
| author |
CHOWDHURY, M. MITRA, J. |
| spellingShingle |
CHOWDHURY, M. MITRA, J. International Journal of Andrology Glycerylphosphorylcholine (GPC) diesterase related alterations in the oxygen consumption profile of rat spermatozoa in differing functional states Urology Reproductive Medicine Endocrinology, Diabetes and Metabolism |
| author_sort |
chowdhury, m. |
| spelling |
CHOWDHURY, M. MITRA, J. 0105-6263 1365-2605 Wiley Urology Reproductive Medicine Endocrinology, Diabetes and Metabolism http://dx.doi.org/10.1111/j.1365-2605.1992.tb01134.x <jats:title>Summary</jats:title><jats:p>The functional interaction of the estrogen‐induced uterine enzyme glycerylphosphorylcholine (GPC) diesterase with epididymal rat sperm before and after incubation under capacitating conditions was investigated indirectly, by measuring the glycerol phosphate (GP) released on enzymatic hydrolysis of GPC and using oxygen consumption as a measure of oxidative phosphorylation by the sperm.</jats:p><jats:p>Freshly released, washed sperm metabolized GP but not GPC. Utilization of GP was found to be inhibited by 2 μM oligomycin but was enhanced dose‐dependently in the presence of the uncoupling agent, 2,4‐dinitrophenol, indicating that the process was essentially similar to that occurring in somatic mitochondria in that it involved electron transport via ubiquinone and the cytochrome system and was coupled to ATP synthesis.</jats:p><jats:p>However, on incubation of sperm under conditions supporting capacitation, which led to a striking increase in oxygen consumption, the presence of GP decreased oxygen uptake significantly, in a manner similar to that occurring in the presence of the glycolyzable substrate, glucose. The implications of these GPC diesterase‐induced metabolic alterations in sperm function are discussed.</jats:p> Glycerylphosphorylcholine (GPC) diesterase related alterations in the oxygen consumption profile of rat spermatozoa in differing functional states International Journal of Andrology |
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International Journal of Andrology |
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| title |
Glycerylphosphorylcholine (GPC) diesterase related alterations in the oxygen consumption profile of rat spermatozoa in differing functional states |
| title_unstemmed |
Glycerylphosphorylcholine (GPC) diesterase related alterations in the oxygen consumption profile of rat spermatozoa in differing functional states |
| title_full |
Glycerylphosphorylcholine (GPC) diesterase related alterations in the oxygen consumption profile of rat spermatozoa in differing functional states |
| title_fullStr |
Glycerylphosphorylcholine (GPC) diesterase related alterations in the oxygen consumption profile of rat spermatozoa in differing functional states |
| title_full_unstemmed |
Glycerylphosphorylcholine (GPC) diesterase related alterations in the oxygen consumption profile of rat spermatozoa in differing functional states |
| title_short |
Glycerylphosphorylcholine (GPC) diesterase related alterations in the oxygen consumption profile of rat spermatozoa in differing functional states |
| title_sort |
glycerylphosphorylcholine (gpc) diesterase related alterations in the oxygen consumption profile of rat spermatozoa in differing functional states |
| topic |
Urology Reproductive Medicine Endocrinology, Diabetes and Metabolism |
| url |
http://dx.doi.org/10.1111/j.1365-2605.1992.tb01134.x |
| publishDate |
1992 |
| physical |
345-354 |
| description |
<jats:title>Summary</jats:title><jats:p>The functional interaction of the estrogen‐induced uterine enzyme glycerylphosphorylcholine (GPC) diesterase with epididymal rat sperm before and after incubation under capacitating conditions was investigated indirectly, by measuring the glycerol phosphate (GP) released on enzymatic hydrolysis of GPC and using oxygen consumption as a measure of oxidative phosphorylation by the sperm.</jats:p><jats:p>Freshly released, washed sperm metabolized GP but not GPC. Utilization of GP was found to be inhibited by 2 μM oligomycin but was enhanced dose‐dependently in the presence of the uncoupling agent, 2,4‐dinitrophenol, indicating that the process was essentially similar to that occurring in somatic mitochondria in that it involved electron transport via ubiquinone and the cytochrome system and was coupled to ATP synthesis.</jats:p><jats:p>However, on incubation of sperm under conditions supporting capacitation, which led to a striking increase in oxygen consumption, the presence of GP decreased oxygen uptake significantly, in a manner similar to that occurring in the presence of the glycolyzable substrate, glucose. The implications of these GPC diesterase‐induced metabolic alterations in sperm function are discussed.</jats:p> |
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| author | CHOWDHURY, M., MITRA, J. |
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| author_sort | chowdhury, m. |
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| container_title | International Journal of Andrology |
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| description | <jats:title>Summary</jats:title><jats:p>The functional interaction of the estrogen‐induced uterine enzyme glycerylphosphorylcholine (GPC) diesterase with epididymal rat sperm before and after incubation under capacitating conditions was investigated indirectly, by measuring the glycerol phosphate (GP) released on enzymatic hydrolysis of GPC and using oxygen consumption as a measure of oxidative phosphorylation by the sperm.</jats:p><jats:p>Freshly released, washed sperm metabolized GP but not GPC. Utilization of GP was found to be inhibited by 2 μM oligomycin but was enhanced dose‐dependently in the presence of the uncoupling agent, 2,4‐dinitrophenol, indicating that the process was essentially similar to that occurring in somatic mitochondria in that it involved electron transport via ubiquinone and the cytochrome system and was coupled to ATP synthesis.</jats:p><jats:p>However, on incubation of sperm under conditions supporting capacitation, which led to a striking increase in oxygen consumption, the presence of GP decreased oxygen uptake significantly, in a manner similar to that occurring in the presence of the glycolyzable substrate, glucose. The implications of these GPC diesterase‐induced metabolic alterations in sperm function are discussed.</jats:p> |
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| imprint | Wiley, 1992 |
| imprint_str_mv | Wiley, 1992 |
| institution | DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3 |
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| spelling | CHOWDHURY, M. MITRA, J. 0105-6263 1365-2605 Wiley Urology Reproductive Medicine Endocrinology, Diabetes and Metabolism http://dx.doi.org/10.1111/j.1365-2605.1992.tb01134.x <jats:title>Summary</jats:title><jats:p>The functional interaction of the estrogen‐induced uterine enzyme glycerylphosphorylcholine (GPC) diesterase with epididymal rat sperm before and after incubation under capacitating conditions was investigated indirectly, by measuring the glycerol phosphate (GP) released on enzymatic hydrolysis of GPC and using oxygen consumption as a measure of oxidative phosphorylation by the sperm.</jats:p><jats:p>Freshly released, washed sperm metabolized GP but not GPC. Utilization of GP was found to be inhibited by 2 μM oligomycin but was enhanced dose‐dependently in the presence of the uncoupling agent, 2,4‐dinitrophenol, indicating that the process was essentially similar to that occurring in somatic mitochondria in that it involved electron transport via ubiquinone and the cytochrome system and was coupled to ATP synthesis.</jats:p><jats:p>However, on incubation of sperm under conditions supporting capacitation, which led to a striking increase in oxygen consumption, the presence of GP decreased oxygen uptake significantly, in a manner similar to that occurring in the presence of the glycolyzable substrate, glucose. The implications of these GPC diesterase‐induced metabolic alterations in sperm function are discussed.</jats:p> Glycerylphosphorylcholine (GPC) diesterase related alterations in the oxygen consumption profile of rat spermatozoa in differing functional states International Journal of Andrology |
| spellingShingle | CHOWDHURY, M., MITRA, J., International Journal of Andrology, Glycerylphosphorylcholine (GPC) diesterase related alterations in the oxygen consumption profile of rat spermatozoa in differing functional states, Urology, Reproductive Medicine, Endocrinology, Diabetes and Metabolism |
| title | Glycerylphosphorylcholine (GPC) diesterase related alterations in the oxygen consumption profile of rat spermatozoa in differing functional states |
| title_full | Glycerylphosphorylcholine (GPC) diesterase related alterations in the oxygen consumption profile of rat spermatozoa in differing functional states |
| title_fullStr | Glycerylphosphorylcholine (GPC) diesterase related alterations in the oxygen consumption profile of rat spermatozoa in differing functional states |
| title_full_unstemmed | Glycerylphosphorylcholine (GPC) diesterase related alterations in the oxygen consumption profile of rat spermatozoa in differing functional states |
| title_short | Glycerylphosphorylcholine (GPC) diesterase related alterations in the oxygen consumption profile of rat spermatozoa in differing functional states |
| title_sort | glycerylphosphorylcholine (gpc) diesterase related alterations in the oxygen consumption profile of rat spermatozoa in differing functional states |
| title_unstemmed | Glycerylphosphorylcholine (GPC) diesterase related alterations in the oxygen consumption profile of rat spermatozoa in differing functional states |
| topic | Urology, Reproductive Medicine, Endocrinology, Diabetes and Metabolism |
| url | http://dx.doi.org/10.1111/j.1365-2605.1992.tb01134.x |