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Role of a xyloglucan‐specific endo‐β‐1,4‐glucanase inhibitor in the interactions of Nicotiana benthamiana with Colletotrichum destructivum, C. orbiculare or Pseudomonas syringae pv...
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| Zeitschriftentitel: | Molecular Plant Pathology |
|---|---|
| Personen und Körperschaften: | , , |
| In: | Molecular Plant Pathology, 9, 2008, 2, S. 191-202 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
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| Schlagwörter: |
| author_facet |
XIE, W. HAO, L. GOODWIN, P. H. XIE, W. HAO, L. GOODWIN, P. H. |
|---|---|
| author |
XIE, W. HAO, L. GOODWIN, P. H. |
| spellingShingle |
XIE, W. HAO, L. GOODWIN, P. H. Molecular Plant Pathology Role of a xyloglucan‐specific endo‐β‐1,4‐glucanase inhibitor in the interactions of Nicotiana benthamiana with Colletotrichum destructivum, C. orbiculare or Pseudomonas syringae pv. tabaci Plant Science Soil Science Agronomy and Crop Science Molecular Biology |
| author_sort |
xie, w. |
| spelling |
XIE, W. HAO, L. GOODWIN, P. H. 1464-6722 1364-3703 Wiley Plant Science Soil Science Agronomy and Crop Science Molecular Biology http://dx.doi.org/10.1111/j.1364-3703.2007.00457.x <jats:title>SUMMARY</jats:title><jats:p>A xyloglucan‐specific endo‐β‐1,4‐glucanase inhibitor cDNA, <jats:italic>NbXEGIP1</jats:italic>, was amplified from diseased leaves of <jats:italic>Nicotiana benthamiana</jats:italic>. The sequence was similar to the tomato xyloglucan‐specific endo‐β‐1,4‐glucanase inhibitor (XEGIP) and tobacco nectarin IV genes that have been described as binding and inactivating fungal Family 12 xyloglucan‐specific endo‐β‐1,4‐glucanases. Expression of<jats:italic> NbXEGIP1</jats:italic> was not detected in healthy leaves, but the gene was induced during the later stages of infection by the fungi <jats:italic>Colletotrichum destructivum</jats:italic> and <jats:italic>C. orbiculare</jats:italic>. Induction of <jats:italic>NbXEGIP1</jats:italic> also occurred during disease development by the bacterium <jats:italic>Pseudomonas syringae</jats:italic> pv. <jats:italic>tabaci</jats:italic> and during the hypersensitive response produced by <jats:italic>P. syringae</jats:italic> pv. <jats:italic>tabaci</jats:italic> expressing<jats:italic> avrPto</jats:italic>. A portion of <jats:italic>NbXEGIP1 </jats:italic>was cloned into a tobacco rattle virus vector for virus‐induced gene silencing in <jats:italic>N. benthamiana</jats:italic>. Silencing <jats:italic>NbXEGIP1</jats:italic> did not affect the interactions with either <jats:italic>Colletotrichum</jats:italic> species but did significantly reduce population levels of <jats:italic>P. syringae</jats:italic> pv. <jats:italic>tabaci</jats:italic> in the compatible interaction and <jats:italic>P. syringae</jats:italic> pv. <jats:italic>tabaci</jats:italic> expressing <jats:italic>avrPto</jats:italic> in the incompatible interaction. In the susceptible response to <jats:italic>P. syringae</jats:italic> pv. <jats:italic>tabaci</jats:italic>, silencing of<jats:italic> NbXEGIP1</jats:italic> also resulted in visibly wilted leaves several hours prior to necrosis, which was not observed in control plants. This was related to a significantly higher level of electrolyte leakage and higher expression of a defensin gene from infected <jats:italic>NbXEGIP1</jats:italic>‐silenced leaves compared with control leaves. Silencing appeared to be specific as it did not affect expression of a related gene, <jats:italic>NbXEGIP2</jats:italic>. <jats:italic>NbXEGIP1</jats:italic> may act as an inhibitor of a bacterial enzyme that degrades the xyloglucan–cellulose plant cell‐wall network, and degradation of the cell wall results in host membrane disruption and signalling of defence responses.</jats:p> Role of a xyloglucan‐specific endo‐β‐1,4‐glucanase inhibitor in the interactions of <i>Nicotiana benthamiana</i> with <i>Colletotrichum destructivum</i>, <i>C. orbiculare</i> or <i>Pseudomonas syringae </i>pv.<i> tabaci</i> Molecular Plant Pathology |
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Molecular Plant Pathology |
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| title |
Role of a xyloglucan‐specific endo‐β‐1,4‐glucanase inhibitor in the interactions of Nicotiana benthamiana with Colletotrichum destructivum, C. orbiculare or Pseudomonas syringae pv. tabaci |
| title_unstemmed |
Role of a xyloglucan‐specific endo‐β‐1,4‐glucanase inhibitor in the interactions of Nicotiana benthamiana with Colletotrichum destructivum, C. orbiculare or Pseudomonas syringae pv. tabaci |
| title_full |
Role of a xyloglucan‐specific endo‐β‐1,4‐glucanase inhibitor in the interactions of Nicotiana benthamiana with Colletotrichum destructivum, C. orbiculare or Pseudomonas syringae pv. tabaci |
| title_fullStr |
Role of a xyloglucan‐specific endo‐β‐1,4‐glucanase inhibitor in the interactions of Nicotiana benthamiana with Colletotrichum destructivum, C. orbiculare or Pseudomonas syringae pv. tabaci |
| title_full_unstemmed |
Role of a xyloglucan‐specific endo‐β‐1,4‐glucanase inhibitor in the interactions of Nicotiana benthamiana with Colletotrichum destructivum, C. orbiculare or Pseudomonas syringae pv. tabaci |
| title_short |
Role of a xyloglucan‐specific endo‐β‐1,4‐glucanase inhibitor in the interactions of Nicotiana benthamiana with Colletotrichum destructivum, C. orbiculare or Pseudomonas syringae pv. tabaci |
| title_sort |
role of a xyloglucan‐specific endo‐β‐1,4‐glucanase inhibitor in the interactions of <i>nicotiana benthamiana</i> with <i>colletotrichum destructivum</i>, <i>c. orbiculare</i> or <i>pseudomonas syringae </i>pv.<i> tabaci</i> |
| topic |
Plant Science Soil Science Agronomy and Crop Science Molecular Biology |
| url |
http://dx.doi.org/10.1111/j.1364-3703.2007.00457.x |
| publishDate |
2008 |
| physical |
191-202 |
| description |
<jats:title>SUMMARY</jats:title><jats:p>A xyloglucan‐specific endo‐β‐1,4‐glucanase inhibitor cDNA, <jats:italic>NbXEGIP1</jats:italic>, was amplified from diseased leaves of <jats:italic>Nicotiana benthamiana</jats:italic>. The sequence was similar to the tomato xyloglucan‐specific endo‐β‐1,4‐glucanase inhibitor (XEGIP) and tobacco nectarin IV genes that have been described as binding and inactivating fungal Family 12 xyloglucan‐specific endo‐β‐1,4‐glucanases. Expression of<jats:italic> NbXEGIP1</jats:italic> was not detected in healthy leaves, but the gene was induced during the later stages of infection by the fungi <jats:italic>Colletotrichum destructivum</jats:italic> and <jats:italic>C. orbiculare</jats:italic>. Induction of <jats:italic>NbXEGIP1</jats:italic> also occurred during disease development by the bacterium <jats:italic>Pseudomonas syringae</jats:italic> pv. <jats:italic>tabaci</jats:italic> and during the hypersensitive response produced by <jats:italic>P. syringae</jats:italic> pv. <jats:italic>tabaci</jats:italic> expressing<jats:italic> avrPto</jats:italic>. A portion of <jats:italic>NbXEGIP1 </jats:italic>was cloned into a tobacco rattle virus vector for virus‐induced gene silencing in <jats:italic>N. benthamiana</jats:italic>. Silencing <jats:italic>NbXEGIP1</jats:italic> did not affect the interactions with either <jats:italic>Colletotrichum</jats:italic> species but did significantly reduce population levels of <jats:italic>P. syringae</jats:italic> pv. <jats:italic>tabaci</jats:italic> in the compatible interaction and <jats:italic>P. syringae</jats:italic> pv. <jats:italic>tabaci</jats:italic> expressing <jats:italic>avrPto</jats:italic> in the incompatible interaction. In the susceptible response to <jats:italic>P. syringae</jats:italic> pv. <jats:italic>tabaci</jats:italic>, silencing of<jats:italic> NbXEGIP1</jats:italic> also resulted in visibly wilted leaves several hours prior to necrosis, which was not observed in control plants. This was related to a significantly higher level of electrolyte leakage and higher expression of a defensin gene from infected <jats:italic>NbXEGIP1</jats:italic>‐silenced leaves compared with control leaves. Silencing appeared to be specific as it did not affect expression of a related gene, <jats:italic>NbXEGIP2</jats:italic>. <jats:italic>NbXEGIP1</jats:italic> may act as an inhibitor of a bacterial enzyme that degrades the xyloglucan–cellulose plant cell‐wall network, and degradation of the cell wall results in host membrane disruption and signalling of defence responses.</jats:p> |
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| description | <jats:title>SUMMARY</jats:title><jats:p>A xyloglucan‐specific endo‐β‐1,4‐glucanase inhibitor cDNA, <jats:italic>NbXEGIP1</jats:italic>, was amplified from diseased leaves of <jats:italic>Nicotiana benthamiana</jats:italic>. The sequence was similar to the tomato xyloglucan‐specific endo‐β‐1,4‐glucanase inhibitor (XEGIP) and tobacco nectarin IV genes that have been described as binding and inactivating fungal Family 12 xyloglucan‐specific endo‐β‐1,4‐glucanases. Expression of<jats:italic> NbXEGIP1</jats:italic> was not detected in healthy leaves, but the gene was induced during the later stages of infection by the fungi <jats:italic>Colletotrichum destructivum</jats:italic> and <jats:italic>C. orbiculare</jats:italic>. Induction of <jats:italic>NbXEGIP1</jats:italic> also occurred during disease development by the bacterium <jats:italic>Pseudomonas syringae</jats:italic> pv. <jats:italic>tabaci</jats:italic> and during the hypersensitive response produced by <jats:italic>P. syringae</jats:italic> pv. <jats:italic>tabaci</jats:italic> expressing<jats:italic> avrPto</jats:italic>. A portion of <jats:italic>NbXEGIP1 </jats:italic>was cloned into a tobacco rattle virus vector for virus‐induced gene silencing in <jats:italic>N. benthamiana</jats:italic>. Silencing <jats:italic>NbXEGIP1</jats:italic> did not affect the interactions with either <jats:italic>Colletotrichum</jats:italic> species but did significantly reduce population levels of <jats:italic>P. syringae</jats:italic> pv. <jats:italic>tabaci</jats:italic> in the compatible interaction and <jats:italic>P. syringae</jats:italic> pv. <jats:italic>tabaci</jats:italic> expressing <jats:italic>avrPto</jats:italic> in the incompatible interaction. In the susceptible response to <jats:italic>P. syringae</jats:italic> pv. <jats:italic>tabaci</jats:italic>, silencing of<jats:italic> NbXEGIP1</jats:italic> also resulted in visibly wilted leaves several hours prior to necrosis, which was not observed in control plants. This was related to a significantly higher level of electrolyte leakage and higher expression of a defensin gene from infected <jats:italic>NbXEGIP1</jats:italic>‐silenced leaves compared with control leaves. Silencing appeared to be specific as it did not affect expression of a related gene, <jats:italic>NbXEGIP2</jats:italic>. <jats:italic>NbXEGIP1</jats:italic> may act as an inhibitor of a bacterial enzyme that degrades the xyloglucan–cellulose plant cell‐wall network, and degradation of the cell wall results in host membrane disruption and signalling of defence responses.</jats:p> |
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| spelling | XIE, W. HAO, L. GOODWIN, P. H. 1464-6722 1364-3703 Wiley Plant Science Soil Science Agronomy and Crop Science Molecular Biology http://dx.doi.org/10.1111/j.1364-3703.2007.00457.x <jats:title>SUMMARY</jats:title><jats:p>A xyloglucan‐specific endo‐β‐1,4‐glucanase inhibitor cDNA, <jats:italic>NbXEGIP1</jats:italic>, was amplified from diseased leaves of <jats:italic>Nicotiana benthamiana</jats:italic>. The sequence was similar to the tomato xyloglucan‐specific endo‐β‐1,4‐glucanase inhibitor (XEGIP) and tobacco nectarin IV genes that have been described as binding and inactivating fungal Family 12 xyloglucan‐specific endo‐β‐1,4‐glucanases. Expression of<jats:italic> NbXEGIP1</jats:italic> was not detected in healthy leaves, but the gene was induced during the later stages of infection by the fungi <jats:italic>Colletotrichum destructivum</jats:italic> and <jats:italic>C. orbiculare</jats:italic>. Induction of <jats:italic>NbXEGIP1</jats:italic> also occurred during disease development by the bacterium <jats:italic>Pseudomonas syringae</jats:italic> pv. <jats:italic>tabaci</jats:italic> and during the hypersensitive response produced by <jats:italic>P. syringae</jats:italic> pv. <jats:italic>tabaci</jats:italic> expressing<jats:italic> avrPto</jats:italic>. A portion of <jats:italic>NbXEGIP1 </jats:italic>was cloned into a tobacco rattle virus vector for virus‐induced gene silencing in <jats:italic>N. benthamiana</jats:italic>. Silencing <jats:italic>NbXEGIP1</jats:italic> did not affect the interactions with either <jats:italic>Colletotrichum</jats:italic> species but did significantly reduce population levels of <jats:italic>P. syringae</jats:italic> pv. <jats:italic>tabaci</jats:italic> in the compatible interaction and <jats:italic>P. syringae</jats:italic> pv. <jats:italic>tabaci</jats:italic> expressing <jats:italic>avrPto</jats:italic> in the incompatible interaction. In the susceptible response to <jats:italic>P. syringae</jats:italic> pv. <jats:italic>tabaci</jats:italic>, silencing of<jats:italic> NbXEGIP1</jats:italic> also resulted in visibly wilted leaves several hours prior to necrosis, which was not observed in control plants. This was related to a significantly higher level of electrolyte leakage and higher expression of a defensin gene from infected <jats:italic>NbXEGIP1</jats:italic>‐silenced leaves compared with control leaves. Silencing appeared to be specific as it did not affect expression of a related gene, <jats:italic>NbXEGIP2</jats:italic>. <jats:italic>NbXEGIP1</jats:italic> may act as an inhibitor of a bacterial enzyme that degrades the xyloglucan–cellulose plant cell‐wall network, and degradation of the cell wall results in host membrane disruption and signalling of defence responses.</jats:p> Role of a xyloglucan‐specific endo‐β‐1,4‐glucanase inhibitor in the interactions of <i>Nicotiana benthamiana</i> with <i>Colletotrichum destructivum</i>, <i>C. orbiculare</i> or <i>Pseudomonas syringae </i>pv.<i> tabaci</i> Molecular Plant Pathology |
| spellingShingle | XIE, W., HAO, L., GOODWIN, P. H., Molecular Plant Pathology, Role of a xyloglucan‐specific endo‐β‐1,4‐glucanase inhibitor in the interactions of Nicotiana benthamiana with Colletotrichum destructivum, C. orbiculare or Pseudomonas syringae pv. tabaci, Plant Science, Soil Science, Agronomy and Crop Science, Molecular Biology |
| title | Role of a xyloglucan‐specific endo‐β‐1,4‐glucanase inhibitor in the interactions of Nicotiana benthamiana with Colletotrichum destructivum, C. orbiculare or Pseudomonas syringae pv. tabaci |
| title_full | Role of a xyloglucan‐specific endo‐β‐1,4‐glucanase inhibitor in the interactions of Nicotiana benthamiana with Colletotrichum destructivum, C. orbiculare or Pseudomonas syringae pv. tabaci |
| title_fullStr | Role of a xyloglucan‐specific endo‐β‐1,4‐glucanase inhibitor in the interactions of Nicotiana benthamiana with Colletotrichum destructivum, C. orbiculare or Pseudomonas syringae pv. tabaci |
| title_full_unstemmed | Role of a xyloglucan‐specific endo‐β‐1,4‐glucanase inhibitor in the interactions of Nicotiana benthamiana with Colletotrichum destructivum, C. orbiculare or Pseudomonas syringae pv. tabaci |
| title_short | Role of a xyloglucan‐specific endo‐β‐1,4‐glucanase inhibitor in the interactions of Nicotiana benthamiana with Colletotrichum destructivum, C. orbiculare or Pseudomonas syringae pv. tabaci |
| title_sort | role of a xyloglucan‐specific endo‐β‐1,4‐glucanase inhibitor in the interactions of <i>nicotiana benthamiana</i> with <i>colletotrichum destructivum</i>, <i>c. orbiculare</i> or <i>pseudomonas syringae </i>pv.<i> tabaci</i> |
| title_unstemmed | Role of a xyloglucan‐specific endo‐β‐1,4‐glucanase inhibitor in the interactions of Nicotiana benthamiana with Colletotrichum destructivum, C. orbiculare or Pseudomonas syringae pv. tabaci |
| topic | Plant Science, Soil Science, Agronomy and Crop Science, Molecular Biology |
| url | http://dx.doi.org/10.1111/j.1364-3703.2007.00457.x |