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Futile cycling of glycogen in Fibrobacter succinogenes as shown by in situ1H‐NMR and 13C‐NMR investigation
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| Zeitschriftentitel: | European Journal of Biochemistry |
|---|---|
| Personen und Körperschaften: | , , , |
| In: | European Journal of Biochemistry, 207, 1992, 1, S. 155-162 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
|
| Schlagwörter: |
| author_facet |
GAUDET, Geneviève FORANO, Evelyne DAUPHIN, Gérard DELORT, Anne‐Marie GAUDET, Geneviève FORANO, Evelyne DAUPHIN, Gérard DELORT, Anne‐Marie |
|---|---|
| author |
GAUDET, Geneviève FORANO, Evelyne DAUPHIN, Gérard DELORT, Anne‐Marie |
| spellingShingle |
GAUDET, Geneviève FORANO, Evelyne DAUPHIN, Gérard DELORT, Anne‐Marie European Journal of Biochemistry Futile cycling of glycogen in Fibrobacter succinogenes as shown by in situ1H‐NMR and 13C‐NMR investigation Biochemistry |
| author_sort |
gaudet, geneviève |
| spelling |
GAUDET, Geneviève FORANO, Evelyne DAUPHIN, Gérard DELORT, Anne‐Marie 0014-2956 1432-1033 Wiley Biochemistry http://dx.doi.org/10.1111/j.1432-1033.1992.tb17032.x <jats:p>Glycogen was synthesized during all the growth phases in the rumen anaerobic cellulolytic bacterium <jats:italic>Fibrobacter succinogenes</jats:italic>. Glycogen synthesis and degradation were monitored using <jats:italic>in situ</jats:italic><jats:sup>13</jats:sup>C and <jats:sup>1</jats:sup>H‐NMR spectroscopy in resting cells of <jats:italic>F. succinogenes</jats:italic>. The cells were incubated at 37°C under anaerobic conditions with [1‐<jats:sup>13</jats:sup>C]glucose and [2‐<jats:sup>13</jats:sup>C]glucose. <jats:sup>1</jats:sup>H‐NMR spectra were used to quantify enrichment by <jats:sup>13</jats:sup>C of metabolism products. Glucose was utilized for energy requirements of the bacterium essentially via the Embden‐Meyerhof pathway, leading to the synthesis of succinate and acetate, while glycogen was stored. From [1‐<jats:sup>13</jats:sup>C]glucose, labeling occurred on C2 of succinate and acetate, and on both C1 and C6 of glycogen, the labeling on C1 being predominant. The C6‐labeling of glycogen may be explained by scrambling and reversal of the glycolytic pathway at the triose‐phosphate and fructose 1,6‐bisphosphate level. When the bacteria were incubated first with [1‐<jats:sup>13</jats:sup>C]glucose, then washed and incubated with [2‐<jats:sup>13</jats:sup>C]glucose, the pattern of <jats:sup>13</jats:sup>C labeling in the products of the metabolism, as shown by <jats:sup>13</jats:sup>C and <jats:sup>1</jats:sup>H‐NMR spectra, indicated that glycogen was degraded at the same time as it was being stored, suggesting futile cycling of glycogen. The hydrolysis of previously stored glycogen can provide, in the presence of glucose, up to 30% of the carbon source for the bacteria.</jats:p> Futile cycling of glycogen in <i>Fibrobacter succinogenes</i> as shown by <i>in situ</i><sup>1</sup>H‐NMR and <sup>13</sup>C‐NMR investigation European Journal of Biochemistry |
| doi_str_mv |
10.1111/j.1432-1033.1992.tb17032.x |
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Online Free |
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Chemie und Pharmazie |
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Wiley, 1992 |
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Wiley, 1992 |
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0014-2956 1432-1033 |
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0014-2956 1432-1033 |
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1992 |
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Wiley |
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European Journal of Biochemistry |
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49 |
| title |
Futile cycling of glycogen in Fibrobacter succinogenes as shown by in situ1H‐NMR and 13C‐NMR investigation |
| title_unstemmed |
Futile cycling of glycogen in Fibrobacter succinogenes as shown by in situ1H‐NMR and 13C‐NMR investigation |
| title_full |
Futile cycling of glycogen in Fibrobacter succinogenes as shown by in situ1H‐NMR and 13C‐NMR investigation |
| title_fullStr |
Futile cycling of glycogen in Fibrobacter succinogenes as shown by in situ1H‐NMR and 13C‐NMR investigation |
| title_full_unstemmed |
Futile cycling of glycogen in Fibrobacter succinogenes as shown by in situ1H‐NMR and 13C‐NMR investigation |
| title_short |
Futile cycling of glycogen in Fibrobacter succinogenes as shown by in situ1H‐NMR and 13C‐NMR investigation |
| title_sort |
futile cycling of glycogen in <i>fibrobacter succinogenes</i> as shown by <i>in situ</i><sup>1</sup>h‐nmr and <sup>13</sup>c‐nmr investigation |
| topic |
Biochemistry |
| url |
http://dx.doi.org/10.1111/j.1432-1033.1992.tb17032.x |
| publishDate |
1992 |
| physical |
155-162 |
| description |
<jats:p>Glycogen was synthesized during all the growth phases in the rumen anaerobic cellulolytic bacterium <jats:italic>Fibrobacter succinogenes</jats:italic>. Glycogen synthesis and degradation were monitored using <jats:italic>in situ</jats:italic><jats:sup>13</jats:sup>C and <jats:sup>1</jats:sup>H‐NMR spectroscopy in resting cells of <jats:italic>F. succinogenes</jats:italic>. The cells were incubated at 37°C under anaerobic conditions with [1‐<jats:sup>13</jats:sup>C]glucose and [2‐<jats:sup>13</jats:sup>C]glucose. <jats:sup>1</jats:sup>H‐NMR spectra were used to quantify enrichment by <jats:sup>13</jats:sup>C of metabolism products. Glucose was utilized for energy requirements of the bacterium essentially via the Embden‐Meyerhof pathway, leading to the synthesis of succinate and acetate, while glycogen was stored. From [1‐<jats:sup>13</jats:sup>C]glucose, labeling occurred on C2 of succinate and acetate, and on both C1 and C6 of glycogen, the labeling on C1 being predominant. The C6‐labeling of glycogen may be explained by scrambling and reversal of the glycolytic pathway at the triose‐phosphate and fructose 1,6‐bisphosphate level. When the bacteria were incubated first with [1‐<jats:sup>13</jats:sup>C]glucose, then washed and incubated with [2‐<jats:sup>13</jats:sup>C]glucose, the pattern of <jats:sup>13</jats:sup>C labeling in the products of the metabolism, as shown by <jats:sup>13</jats:sup>C and <jats:sup>1</jats:sup>H‐NMR spectra, indicated that glycogen was degraded at the same time as it was being stored, suggesting futile cycling of glycogen. The hydrolysis of previously stored glycogen can provide, in the presence of glucose, up to 30% of the carbon source for the bacteria.</jats:p> |
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| author | GAUDET, Geneviève, FORANO, Evelyne, DAUPHIN, Gérard, DELORT, Anne‐Marie |
| author_facet | GAUDET, Geneviève, FORANO, Evelyne, DAUPHIN, Gérard, DELORT, Anne‐Marie, GAUDET, Geneviève, FORANO, Evelyne, DAUPHIN, Gérard, DELORT, Anne‐Marie |
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| description | <jats:p>Glycogen was synthesized during all the growth phases in the rumen anaerobic cellulolytic bacterium <jats:italic>Fibrobacter succinogenes</jats:italic>. Glycogen synthesis and degradation were monitored using <jats:italic>in situ</jats:italic><jats:sup>13</jats:sup>C and <jats:sup>1</jats:sup>H‐NMR spectroscopy in resting cells of <jats:italic>F. succinogenes</jats:italic>. The cells were incubated at 37°C under anaerobic conditions with [1‐<jats:sup>13</jats:sup>C]glucose and [2‐<jats:sup>13</jats:sup>C]glucose. <jats:sup>1</jats:sup>H‐NMR spectra were used to quantify enrichment by <jats:sup>13</jats:sup>C of metabolism products. Glucose was utilized for energy requirements of the bacterium essentially via the Embden‐Meyerhof pathway, leading to the synthesis of succinate and acetate, while glycogen was stored. From [1‐<jats:sup>13</jats:sup>C]glucose, labeling occurred on C2 of succinate and acetate, and on both C1 and C6 of glycogen, the labeling on C1 being predominant. The C6‐labeling of glycogen may be explained by scrambling and reversal of the glycolytic pathway at the triose‐phosphate and fructose 1,6‐bisphosphate level. When the bacteria were incubated first with [1‐<jats:sup>13</jats:sup>C]glucose, then washed and incubated with [2‐<jats:sup>13</jats:sup>C]glucose, the pattern of <jats:sup>13</jats:sup>C labeling in the products of the metabolism, as shown by <jats:sup>13</jats:sup>C and <jats:sup>1</jats:sup>H‐NMR spectra, indicated that glycogen was degraded at the same time as it was being stored, suggesting futile cycling of glycogen. The hydrolysis of previously stored glycogen can provide, in the presence of glucose, up to 30% of the carbon source for the bacteria.</jats:p> |
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| imprint | Wiley, 1992 |
| imprint_str_mv | Wiley, 1992 |
| institution | DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275 |
| issn | 0014-2956, 1432-1033 |
| issn_str_mv | 0014-2956, 1432-1033 |
| language | English |
| last_indexed | 2024-03-01T15:46:46.492Z |
| match_str | gaudet1992futilecyclingofglycogeninfibrobactersuccinogenesasshownbyinsitu1hnmrand13cnmrinvestigation |
| mega_collection | Wiley (CrossRef) |
| physical | 155-162 |
| publishDate | 1992 |
| publishDateSort | 1992 |
| publisher | Wiley |
| record_format | ai |
| recordtype | ai |
| series | European Journal of Biochemistry |
| source_id | 49 |
| spelling | GAUDET, Geneviève FORANO, Evelyne DAUPHIN, Gérard DELORT, Anne‐Marie 0014-2956 1432-1033 Wiley Biochemistry http://dx.doi.org/10.1111/j.1432-1033.1992.tb17032.x <jats:p>Glycogen was synthesized during all the growth phases in the rumen anaerobic cellulolytic bacterium <jats:italic>Fibrobacter succinogenes</jats:italic>. Glycogen synthesis and degradation were monitored using <jats:italic>in situ</jats:italic><jats:sup>13</jats:sup>C and <jats:sup>1</jats:sup>H‐NMR spectroscopy in resting cells of <jats:italic>F. succinogenes</jats:italic>. The cells were incubated at 37°C under anaerobic conditions with [1‐<jats:sup>13</jats:sup>C]glucose and [2‐<jats:sup>13</jats:sup>C]glucose. <jats:sup>1</jats:sup>H‐NMR spectra were used to quantify enrichment by <jats:sup>13</jats:sup>C of metabolism products. Glucose was utilized for energy requirements of the bacterium essentially via the Embden‐Meyerhof pathway, leading to the synthesis of succinate and acetate, while glycogen was stored. From [1‐<jats:sup>13</jats:sup>C]glucose, labeling occurred on C2 of succinate and acetate, and on both C1 and C6 of glycogen, the labeling on C1 being predominant. The C6‐labeling of glycogen may be explained by scrambling and reversal of the glycolytic pathway at the triose‐phosphate and fructose 1,6‐bisphosphate level. When the bacteria were incubated first with [1‐<jats:sup>13</jats:sup>C]glucose, then washed and incubated with [2‐<jats:sup>13</jats:sup>C]glucose, the pattern of <jats:sup>13</jats:sup>C labeling in the products of the metabolism, as shown by <jats:sup>13</jats:sup>C and <jats:sup>1</jats:sup>H‐NMR spectra, indicated that glycogen was degraded at the same time as it was being stored, suggesting futile cycling of glycogen. The hydrolysis of previously stored glycogen can provide, in the presence of glucose, up to 30% of the carbon source for the bacteria.</jats:p> Futile cycling of glycogen in <i>Fibrobacter succinogenes</i> as shown by <i>in situ</i><sup>1</sup>H‐NMR and <sup>13</sup>C‐NMR investigation European Journal of Biochemistry |
| spellingShingle | GAUDET, Geneviève, FORANO, Evelyne, DAUPHIN, Gérard, DELORT, Anne‐Marie, European Journal of Biochemistry, Futile cycling of glycogen in Fibrobacter succinogenes as shown by in situ1H‐NMR and 13C‐NMR investigation, Biochemistry |
| title | Futile cycling of glycogen in Fibrobacter succinogenes as shown by in situ1H‐NMR and 13C‐NMR investigation |
| title_full | Futile cycling of glycogen in Fibrobacter succinogenes as shown by in situ1H‐NMR and 13C‐NMR investigation |
| title_fullStr | Futile cycling of glycogen in Fibrobacter succinogenes as shown by in situ1H‐NMR and 13C‐NMR investigation |
| title_full_unstemmed | Futile cycling of glycogen in Fibrobacter succinogenes as shown by in situ1H‐NMR and 13C‐NMR investigation |
| title_short | Futile cycling of glycogen in Fibrobacter succinogenes as shown by in situ1H‐NMR and 13C‐NMR investigation |
| title_sort | futile cycling of glycogen in <i>fibrobacter succinogenes</i> as shown by <i>in situ</i><sup>1</sup>h‐nmr and <sup>13</sup>c‐nmr investigation |
| title_unstemmed | Futile cycling of glycogen in Fibrobacter succinogenes as shown by in situ1H‐NMR and 13C‐NMR investigation |
| topic | Biochemistry |
| url | http://dx.doi.org/10.1111/j.1432-1033.1992.tb17032.x |