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In vitro Synthesis of CDP‐D‐Abequose Using Salmonella Enzymes of Cloned rfb Genes : Production of CDP‐6‐Deoxy‐D‐Xylo ‐4‐Hexulose, CDP‐3,6‐Dideoxy‐D‐Xylo ‐4‐Hexulose and CDP‐3,6‐Did...
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| Zeitschriftentitel: | European Journal of Biochemistry |
|---|---|
| Personen und Körperschaften: | , , , |
| In: | European Journal of Biochemistry, 225, 1994, 3, S. 863-872 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
|
| Schlagwörter: |
| author_facet |
Lindqvist, Lennart Schweda, K. H. Reeves, Peter R. Lindberg, Alf A. Lindqvist, Lennart Schweda, K. H. Reeves, Peter R. Lindberg, Alf A. |
|---|---|
| author |
Lindqvist, Lennart Schweda, K. H. Reeves, Peter R. Lindberg, Alf A. |
| spellingShingle |
Lindqvist, Lennart Schweda, K. H. Reeves, Peter R. Lindberg, Alf A. European Journal of Biochemistry In vitro Synthesis of CDP‐D‐Abequose Using Salmonella Enzymes of Cloned rfb Genes : Production of CDP‐6‐Deoxy‐D‐Xylo ‐4‐Hexulose, CDP‐3,6‐Dideoxy‐D‐Xylo ‐4‐Hexulose and CDP‐3,6‐Dideoxy‐D‐Galactose, and Isolation by HPLC Biochemistry |
| author_sort |
lindqvist, lennart |
| spelling |
Lindqvist, Lennart Schweda, K. H. Reeves, Peter R. Lindberg, Alf A. 0014-2956 1432-1033 Wiley Biochemistry http://dx.doi.org/10.1111/j.1432-1033.1994.0863b.x <jats:p> <jats:italic>In vitro</jats:italic> enzymic synthesis of CDP‐D‐abequose, CDP‐D‐[U‐<jats:sup>14</jats:sup>C]abequose, CDP‐6‐deoxy‐D‐<jats:italic>xylo</jats:italic> ‐4‐hexulose and CDP‐3,6‐dideoxy‐D‐<jats:italic>xylo</jats:italic> ‐4‐hexulose was achieved using enzymes from cell extracts of cultures of <jats:italic>Escherichia coli</jats:italic> strains harbouring and expressing genes of the <jats:italic>rfb</jats:italic> gene cluster of <jats:italic>Salmonella enterica</jats:italic> LT2. From an initial synthesis step, CDP‐6‐deoxy‐D‐<jats:italic>xylo</jats:italic> ‐4‐hexulose was isolated after 30 min reaction, using CDP‐D‐glucose, NAD and CDP‐glucose 4,6‐dehydratase, followed by protein precipitation and desalting by gel chromatography (yield 90.6%). From that intermediate, CDP‐3,6‐dideoxy‐D‐<jats:italic>xylo</jats:italic> ‐4‐hexulose was produced in a reaction using NADH and a crude extract containing the required enzymes. CDP‐D‐abequose synthesis was performed either in the presence of excess NADH and NADPH or using an enzymic system which regenerates low concentrations of the coenzymes. In a two‐step reaction, CDP‐D‐glucose was first converted to CDP‐6‐deoxy‐D‐<jats:italic>xylo</jats:italic> ‐4‐hexulose, then, following addition of the required coenzymes and enzymes, CDP‐D‐abequose was formed from this intermediary product in a 1‐h incubation. Starting from 250 mg CDP‐D‐glucose, the molar yield of CDP‐D‐abequose after protein precipitation and HPLC was 82%, corresponding to more than 200 mg. CDP‐D‐[U‐<jats:sup>14</jats:sup>C]abequose was synthesised from α‐D‐[U<jats:sup>14</jats:sup>C]glucose 1‐phosphate and CTP using purified glucose‐1‐phosphate cytidylyltransferase in a reaction preceding the later steps. GC‐MS and NMR revealed that the hexose part of the end product was 3,6‐dideoxy‐D‐galactose (abequose) and that the corresponding intermediates were 4‐keto‐6‐deoxy‐D‐<jats:italic>xylo</jats:italic> ‐hexose and 4‐keto‐3,6‐dideoxy‐D‐<jats:italic>xylo</jats:italic> ‐hexose, respectively. The synthesized CDP‐6‐deoxy‐D‐<jats:italic>xylo</jats:italic> ‐4‐hexulose exhibited the characteristic ultraviolet light absorption at 318 nm but no corresponding absorption was found for CDP‐3,6‐dideoxy‐D‐<jats:italic>xylo</jats:italic> ‐4‐hexulose. A HPLC technique, where the four CDP‐sugars were baseline separated, was developed and used for enzyme assays and for the analysis of synthesized products.</jats:p> Production of CDP‐6‐Deoxy‐D‐<i>Xylo</i> ‐4‐Hexulose, CDP‐3,6‐Dideoxy‐D‐<i>Xylo</i> ‐4‐Hexulose and CDP‐3,6‐Dideoxy‐D‐Galactose, and Isolation by HPLC <i>In vitro</i> Synthesis of CDP‐D‐Abequose Using <i>Salmonella</i> Enzymes of Cloned <i>rfb</i> Genes : Production of CDP‐6‐Deoxy‐D‐<i>Xylo</i> ‐4‐Hexulose, CDP‐3,6‐Dideoxy‐D‐<i>Xylo</i> ‐4‐Hexulose and CDP‐3,6‐Dideoxy‐D‐Galactose, and Isolation by HPLC European Journal of Biochemistry |
| doi_str_mv |
10.1111/j.1432-1033.1994.0863b.x |
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Online Free |
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Chemie und Pharmazie |
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ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTExMS9qLjE0MzItMTAzMy4xOTk0LjA4NjNiLng |
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DE-L229 DE-D275 DE-Bn3 DE-Brt1 DE-Zwi2 DE-D161 DE-Gla1 DE-Zi4 DE-15 DE-Pl11 DE-Rs1 DE-105 DE-14 DE-Ch1 |
| imprint |
Wiley, 1994 |
| imprint_str_mv |
Wiley, 1994 |
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0014-2956 1432-1033 |
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0014-2956 1432-1033 |
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English |
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Wiley (CrossRef) |
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lindqvist1994invitrosynthesisofcdpdabequoseusingsalmonellaenzymesofclonedrfbgenesproductionofcdp6deoxydxylo4hexulosecdp36dideoxydxylo4hexuloseandcdp36dideoxydgalactoseandisolationbyhplcproductionofcdp6deoxydixyloi4hexulosecdp36dideoxydixyloi4hexuloseandcdp36dideoxydgalactoseandisolationbyhplc |
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1994 |
| publisher |
Wiley |
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ai |
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ai |
| series |
European Journal of Biochemistry |
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49 |
| title_sub |
Production of CDP‐6‐Deoxy‐D‐<i>Xylo</i> ‐4‐Hexulose, CDP‐3,6‐Dideoxy‐D‐<i>Xylo</i> ‐4‐Hexulose and CDP‐3,6‐Dideoxy‐D‐Galactose, and Isolation by HPLC |
| title |
In vitro Synthesis of CDP‐D‐Abequose Using Salmonella Enzymes of Cloned rfb Genes : Production of CDP‐6‐Deoxy‐D‐Xylo ‐4‐Hexulose, CDP‐3,6‐Dideoxy‐D‐Xylo ‐4‐Hexulose and CDP‐3,6‐Dideoxy‐D‐Galactose, and Isolation by HPLC |
| title_unstemmed |
In vitro Synthesis of CDP‐D‐Abequose Using Salmonella Enzymes of Cloned rfb Genes : Production of CDP‐6‐Deoxy‐D‐Xylo ‐4‐Hexulose, CDP‐3,6‐Dideoxy‐D‐Xylo ‐4‐Hexulose and CDP‐3,6‐Dideoxy‐D‐Galactose, and Isolation by HPLC |
| title_full |
In vitro Synthesis of CDP‐D‐Abequose Using Salmonella Enzymes of Cloned rfb Genes : Production of CDP‐6‐Deoxy‐D‐Xylo ‐4‐Hexulose, CDP‐3,6‐Dideoxy‐D‐Xylo ‐4‐Hexulose and CDP‐3,6‐Dideoxy‐D‐Galactose, and Isolation by HPLC |
| title_fullStr |
In vitro Synthesis of CDP‐D‐Abequose Using Salmonella Enzymes of Cloned rfb Genes : Production of CDP‐6‐Deoxy‐D‐Xylo ‐4‐Hexulose, CDP‐3,6‐Dideoxy‐D‐Xylo ‐4‐Hexulose and CDP‐3,6‐Dideoxy‐D‐Galactose, and Isolation by HPLC |
| title_full_unstemmed |
In vitro Synthesis of CDP‐D‐Abequose Using Salmonella Enzymes of Cloned rfb Genes : Production of CDP‐6‐Deoxy‐D‐Xylo ‐4‐Hexulose, CDP‐3,6‐Dideoxy‐D‐Xylo ‐4‐Hexulose and CDP‐3,6‐Dideoxy‐D‐Galactose, and Isolation by HPLC |
| title_short |
In vitro Synthesis of CDP‐D‐Abequose Using Salmonella Enzymes of Cloned rfb Genes : Production of CDP‐6‐Deoxy‐D‐Xylo ‐4‐Hexulose, CDP‐3,6‐Dideoxy‐D‐Xylo ‐4‐Hexulose and CDP‐3,6‐Dideoxy‐D‐Galactose, and Isolation by HPLC |
| title_sort |
<i>in vitro</i> synthesis of cdp‐d‐abequose using <i>salmonella</i> enzymes of cloned <i>rfb</i> genes : production of cdp‐6‐deoxy‐d‐<i>xylo</i> ‐4‐hexulose, cdp‐3,6‐dideoxy‐d‐<i>xylo</i> ‐4‐hexulose and cdp‐3,6‐dideoxy‐d‐galactose, and isolation by hplc |
| topic |
Biochemistry |
| url |
http://dx.doi.org/10.1111/j.1432-1033.1994.0863b.x |
| publishDate |
1994 |
| physical |
863-872 |
| description |
<jats:p> <jats:italic>In vitro</jats:italic> enzymic synthesis of CDP‐D‐abequose, CDP‐D‐[U‐<jats:sup>14</jats:sup>C]abequose, CDP‐6‐deoxy‐D‐<jats:italic>xylo</jats:italic> ‐4‐hexulose and CDP‐3,6‐dideoxy‐D‐<jats:italic>xylo</jats:italic> ‐4‐hexulose was achieved using enzymes from cell extracts of cultures of <jats:italic>Escherichia coli</jats:italic> strains harbouring and expressing genes of the <jats:italic>rfb</jats:italic> gene cluster of <jats:italic>Salmonella enterica</jats:italic> LT2. From an initial synthesis step, CDP‐6‐deoxy‐D‐<jats:italic>xylo</jats:italic> ‐4‐hexulose was isolated after 30 min reaction, using CDP‐D‐glucose, NAD and CDP‐glucose 4,6‐dehydratase, followed by protein precipitation and desalting by gel chromatography (yield 90.6%). From that intermediate, CDP‐3,6‐dideoxy‐D‐<jats:italic>xylo</jats:italic> ‐4‐hexulose was produced in a reaction using NADH and a crude extract containing the required enzymes. CDP‐D‐abequose synthesis was performed either in the presence of excess NADH and NADPH or using an enzymic system which regenerates low concentrations of the coenzymes. In a two‐step reaction, CDP‐D‐glucose was first converted to CDP‐6‐deoxy‐D‐<jats:italic>xylo</jats:italic> ‐4‐hexulose, then, following addition of the required coenzymes and enzymes, CDP‐D‐abequose was formed from this intermediary product in a 1‐h incubation. Starting from 250 mg CDP‐D‐glucose, the molar yield of CDP‐D‐abequose after protein precipitation and HPLC was 82%, corresponding to more than 200 mg. CDP‐D‐[U‐<jats:sup>14</jats:sup>C]abequose was synthesised from α‐D‐[U<jats:sup>14</jats:sup>C]glucose 1‐phosphate and CTP using purified glucose‐1‐phosphate cytidylyltransferase in a reaction preceding the later steps. GC‐MS and NMR revealed that the hexose part of the end product was 3,6‐dideoxy‐D‐galactose (abequose) and that the corresponding intermediates were 4‐keto‐6‐deoxy‐D‐<jats:italic>xylo</jats:italic> ‐hexose and 4‐keto‐3,6‐dideoxy‐D‐<jats:italic>xylo</jats:italic> ‐hexose, respectively. The synthesized CDP‐6‐deoxy‐D‐<jats:italic>xylo</jats:italic> ‐4‐hexulose exhibited the characteristic ultraviolet light absorption at 318 nm but no corresponding absorption was found for CDP‐3,6‐dideoxy‐D‐<jats:italic>xylo</jats:italic> ‐4‐hexulose. A HPLC technique, where the four CDP‐sugars were baseline separated, was developed and used for enzyme assays and for the analysis of synthesized products.</jats:p> |
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| author | Lindqvist, Lennart, Schweda, K. H., Reeves, Peter R., Lindberg, Alf A. |
| author_facet | Lindqvist, Lennart, Schweda, K. H., Reeves, Peter R., Lindberg, Alf A., Lindqvist, Lennart, Schweda, K. H., Reeves, Peter R., Lindberg, Alf A. |
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| description | <jats:p> <jats:italic>In vitro</jats:italic> enzymic synthesis of CDP‐D‐abequose, CDP‐D‐[U‐<jats:sup>14</jats:sup>C]abequose, CDP‐6‐deoxy‐D‐<jats:italic>xylo</jats:italic> ‐4‐hexulose and CDP‐3,6‐dideoxy‐D‐<jats:italic>xylo</jats:italic> ‐4‐hexulose was achieved using enzymes from cell extracts of cultures of <jats:italic>Escherichia coli</jats:italic> strains harbouring and expressing genes of the <jats:italic>rfb</jats:italic> gene cluster of <jats:italic>Salmonella enterica</jats:italic> LT2. From an initial synthesis step, CDP‐6‐deoxy‐D‐<jats:italic>xylo</jats:italic> ‐4‐hexulose was isolated after 30 min reaction, using CDP‐D‐glucose, NAD and CDP‐glucose 4,6‐dehydratase, followed by protein precipitation and desalting by gel chromatography (yield 90.6%). From that intermediate, CDP‐3,6‐dideoxy‐D‐<jats:italic>xylo</jats:italic> ‐4‐hexulose was produced in a reaction using NADH and a crude extract containing the required enzymes. CDP‐D‐abequose synthesis was performed either in the presence of excess NADH and NADPH or using an enzymic system which regenerates low concentrations of the coenzymes. In a two‐step reaction, CDP‐D‐glucose was first converted to CDP‐6‐deoxy‐D‐<jats:italic>xylo</jats:italic> ‐4‐hexulose, then, following addition of the required coenzymes and enzymes, CDP‐D‐abequose was formed from this intermediary product in a 1‐h incubation. Starting from 250 mg CDP‐D‐glucose, the molar yield of CDP‐D‐abequose after protein precipitation and HPLC was 82%, corresponding to more than 200 mg. CDP‐D‐[U‐<jats:sup>14</jats:sup>C]abequose was synthesised from α‐D‐[U<jats:sup>14</jats:sup>C]glucose 1‐phosphate and CTP using purified glucose‐1‐phosphate cytidylyltransferase in a reaction preceding the later steps. GC‐MS and NMR revealed that the hexose part of the end product was 3,6‐dideoxy‐D‐galactose (abequose) and that the corresponding intermediates were 4‐keto‐6‐deoxy‐D‐<jats:italic>xylo</jats:italic> ‐hexose and 4‐keto‐3,6‐dideoxy‐D‐<jats:italic>xylo</jats:italic> ‐hexose, respectively. The synthesized CDP‐6‐deoxy‐D‐<jats:italic>xylo</jats:italic> ‐4‐hexulose exhibited the characteristic ultraviolet light absorption at 318 nm but no corresponding absorption was found for CDP‐3,6‐dideoxy‐D‐<jats:italic>xylo</jats:italic> ‐4‐hexulose. A HPLC technique, where the four CDP‐sugars were baseline separated, was developed and used for enzyme assays and for the analysis of synthesized products.</jats:p> |
| doi_str_mv | 10.1111/j.1432-1033.1994.0863b.x |
| facet_avail | Online, Free |
| finc_class_facet | Chemie und Pharmazie |
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| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTExMS9qLjE0MzItMTAzMy4xOTk0LjA4NjNiLng |
| imprint | Wiley, 1994 |
| imprint_str_mv | Wiley, 1994 |
| institution | DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1 |
| issn | 0014-2956, 1432-1033 |
| issn_str_mv | 0014-2956, 1432-1033 |
| language | English |
| last_indexed | 2024-03-01T15:43:25.77Z |
| match_str | lindqvist1994invitrosynthesisofcdpdabequoseusingsalmonellaenzymesofclonedrfbgenesproductionofcdp6deoxydxylo4hexulosecdp36dideoxydxylo4hexuloseandcdp36dideoxydgalactoseandisolationbyhplcproductionofcdp6deoxydixyloi4hexulosecdp36dideoxydixyloi4hexuloseandcdp36dideoxydgalactoseandisolationbyhplc |
| mega_collection | Wiley (CrossRef) |
| physical | 863-872 |
| publishDate | 1994 |
| publishDateSort | 1994 |
| publisher | Wiley |
| record_format | ai |
| recordtype | ai |
| series | European Journal of Biochemistry |
| source_id | 49 |
| spelling | Lindqvist, Lennart Schweda, K. H. Reeves, Peter R. Lindberg, Alf A. 0014-2956 1432-1033 Wiley Biochemistry http://dx.doi.org/10.1111/j.1432-1033.1994.0863b.x <jats:p> <jats:italic>In vitro</jats:italic> enzymic synthesis of CDP‐D‐abequose, CDP‐D‐[U‐<jats:sup>14</jats:sup>C]abequose, CDP‐6‐deoxy‐D‐<jats:italic>xylo</jats:italic> ‐4‐hexulose and CDP‐3,6‐dideoxy‐D‐<jats:italic>xylo</jats:italic> ‐4‐hexulose was achieved using enzymes from cell extracts of cultures of <jats:italic>Escherichia coli</jats:italic> strains harbouring and expressing genes of the <jats:italic>rfb</jats:italic> gene cluster of <jats:italic>Salmonella enterica</jats:italic> LT2. From an initial synthesis step, CDP‐6‐deoxy‐D‐<jats:italic>xylo</jats:italic> ‐4‐hexulose was isolated after 30 min reaction, using CDP‐D‐glucose, NAD and CDP‐glucose 4,6‐dehydratase, followed by protein precipitation and desalting by gel chromatography (yield 90.6%). From that intermediate, CDP‐3,6‐dideoxy‐D‐<jats:italic>xylo</jats:italic> ‐4‐hexulose was produced in a reaction using NADH and a crude extract containing the required enzymes. CDP‐D‐abequose synthesis was performed either in the presence of excess NADH and NADPH or using an enzymic system which regenerates low concentrations of the coenzymes. In a two‐step reaction, CDP‐D‐glucose was first converted to CDP‐6‐deoxy‐D‐<jats:italic>xylo</jats:italic> ‐4‐hexulose, then, following addition of the required coenzymes and enzymes, CDP‐D‐abequose was formed from this intermediary product in a 1‐h incubation. Starting from 250 mg CDP‐D‐glucose, the molar yield of CDP‐D‐abequose after protein precipitation and HPLC was 82%, corresponding to more than 200 mg. CDP‐D‐[U‐<jats:sup>14</jats:sup>C]abequose was synthesised from α‐D‐[U<jats:sup>14</jats:sup>C]glucose 1‐phosphate and CTP using purified glucose‐1‐phosphate cytidylyltransferase in a reaction preceding the later steps. GC‐MS and NMR revealed that the hexose part of the end product was 3,6‐dideoxy‐D‐galactose (abequose) and that the corresponding intermediates were 4‐keto‐6‐deoxy‐D‐<jats:italic>xylo</jats:italic> ‐hexose and 4‐keto‐3,6‐dideoxy‐D‐<jats:italic>xylo</jats:italic> ‐hexose, respectively. The synthesized CDP‐6‐deoxy‐D‐<jats:italic>xylo</jats:italic> ‐4‐hexulose exhibited the characteristic ultraviolet light absorption at 318 nm but no corresponding absorption was found for CDP‐3,6‐dideoxy‐D‐<jats:italic>xylo</jats:italic> ‐4‐hexulose. A HPLC technique, where the four CDP‐sugars were baseline separated, was developed and used for enzyme assays and for the analysis of synthesized products.</jats:p> Production of CDP‐6‐Deoxy‐D‐<i>Xylo</i> ‐4‐Hexulose, CDP‐3,6‐Dideoxy‐D‐<i>Xylo</i> ‐4‐Hexulose and CDP‐3,6‐Dideoxy‐D‐Galactose, and Isolation by HPLC <i>In vitro</i> Synthesis of CDP‐D‐Abequose Using <i>Salmonella</i> Enzymes of Cloned <i>rfb</i> Genes : Production of CDP‐6‐Deoxy‐D‐<i>Xylo</i> ‐4‐Hexulose, CDP‐3,6‐Dideoxy‐D‐<i>Xylo</i> ‐4‐Hexulose and CDP‐3,6‐Dideoxy‐D‐Galactose, and Isolation by HPLC European Journal of Biochemistry |
| spellingShingle | Lindqvist, Lennart, Schweda, K. H., Reeves, Peter R., Lindberg, Alf A., European Journal of Biochemistry, In vitro Synthesis of CDP‐D‐Abequose Using Salmonella Enzymes of Cloned rfb Genes : Production of CDP‐6‐Deoxy‐D‐Xylo ‐4‐Hexulose, CDP‐3,6‐Dideoxy‐D‐Xylo ‐4‐Hexulose and CDP‐3,6‐Dideoxy‐D‐Galactose, and Isolation by HPLC, Biochemistry |
| title | In vitro Synthesis of CDP‐D‐Abequose Using Salmonella Enzymes of Cloned rfb Genes : Production of CDP‐6‐Deoxy‐D‐Xylo ‐4‐Hexulose, CDP‐3,6‐Dideoxy‐D‐Xylo ‐4‐Hexulose and CDP‐3,6‐Dideoxy‐D‐Galactose, and Isolation by HPLC |
| title_full | In vitro Synthesis of CDP‐D‐Abequose Using Salmonella Enzymes of Cloned rfb Genes : Production of CDP‐6‐Deoxy‐D‐Xylo ‐4‐Hexulose, CDP‐3,6‐Dideoxy‐D‐Xylo ‐4‐Hexulose and CDP‐3,6‐Dideoxy‐D‐Galactose, and Isolation by HPLC |
| title_fullStr | In vitro Synthesis of CDP‐D‐Abequose Using Salmonella Enzymes of Cloned rfb Genes : Production of CDP‐6‐Deoxy‐D‐Xylo ‐4‐Hexulose, CDP‐3,6‐Dideoxy‐D‐Xylo ‐4‐Hexulose and CDP‐3,6‐Dideoxy‐D‐Galactose, and Isolation by HPLC |
| title_full_unstemmed | In vitro Synthesis of CDP‐D‐Abequose Using Salmonella Enzymes of Cloned rfb Genes : Production of CDP‐6‐Deoxy‐D‐Xylo ‐4‐Hexulose, CDP‐3,6‐Dideoxy‐D‐Xylo ‐4‐Hexulose and CDP‐3,6‐Dideoxy‐D‐Galactose, and Isolation by HPLC |
| title_short | In vitro Synthesis of CDP‐D‐Abequose Using Salmonella Enzymes of Cloned rfb Genes : Production of CDP‐6‐Deoxy‐D‐Xylo ‐4‐Hexulose, CDP‐3,6‐Dideoxy‐D‐Xylo ‐4‐Hexulose and CDP‐3,6‐Dideoxy‐D‐Galactose, and Isolation by HPLC |
| title_sort | <i>in vitro</i> synthesis of cdp‐d‐abequose using <i>salmonella</i> enzymes of cloned <i>rfb</i> genes : production of cdp‐6‐deoxy‐d‐<i>xylo</i> ‐4‐hexulose, cdp‐3,6‐dideoxy‐d‐<i>xylo</i> ‐4‐hexulose and cdp‐3,6‐dideoxy‐d‐galactose, and isolation by hplc |
| title_sub | Production of CDP‐6‐Deoxy‐D‐<i>Xylo</i> ‐4‐Hexulose, CDP‐3,6‐Dideoxy‐D‐<i>Xylo</i> ‐4‐Hexulose and CDP‐3,6‐Dideoxy‐D‐Galactose, and Isolation by HPLC |
| title_unstemmed | In vitro Synthesis of CDP‐D‐Abequose Using Salmonella Enzymes of Cloned rfb Genes : Production of CDP‐6‐Deoxy‐D‐Xylo ‐4‐Hexulose, CDP‐3,6‐Dideoxy‐D‐Xylo ‐4‐Hexulose and CDP‐3,6‐Dideoxy‐D‐Galactose, and Isolation by HPLC |
| topic | Biochemistry |
| url | http://dx.doi.org/10.1111/j.1432-1033.1994.0863b.x |