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Upregulated expression of monocyte chemoattractant protein‐1 in human periodontal ligament cells induced by interleukin‐1β
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| Zeitschriftentitel: | Australian Dental Journal |
|---|---|
| Personen und Körperschaften: | , |
| In: | Australian Dental Journal, 60, 2015, 3, S. 382-389 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
|
| Schlagwörter: |
| author_facet |
Jin, J Cao, J Jin, J Cao, J |
|---|---|
| author |
Jin, J Cao, J |
| spellingShingle |
Jin, J Cao, J Australian Dental Journal Upregulated expression of monocyte chemoattractant protein‐1 in human periodontal ligament cells induced by interleukin‐1β General Dentistry |
| author_sort |
jin, j |
| spelling |
Jin, J Cao, J 0045-0421 1834-7819 Wiley General Dentistry http://dx.doi.org/10.1111/adj.12234 <jats:title>Abstract</jats:title><jats:sec><jats:title>Background</jats:title><jats:p>Root resorption during orthodontic treatment is a complex and sterile inflammatory process, characterized by the recruitment of mononuclear cells in the local periodontal ligament. This study aimed to investigate whether interleukin (<jats:styled-content style="fixed-case">IL</jats:styled-content>)‐1β could induce the migration of monocytes through upregulating monocyte chemoattractant protein (<jats:styled-content style="fixed-case">MCP</jats:styled-content>)‐1 expression in human periodontal ligament cells.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>Human periodontal ligament cells were cultured in medium containing various <jats:styled-content style="fixed-case">IL</jats:styled-content>‐1β concentrations. After 24 hours of incubation, the messenger <jats:styled-content style="fixed-case">RNA</jats:styled-content> (<jats:styled-content style="fixed-case">mRNA</jats:styled-content>) and <jats:styled-content style="fixed-case">MCP</jats:styled-content>‐1 protein in periodontal ligament cells were detected by reverse transcriptase–polymerase chain reaction and Western blot analysis, respectively. The effect of supernatants of periodontal ligament cells on <jats:styled-content style="fixed-case">THP</jats:styled-content>‐1 cells was analysed via migration assay.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p><jats:styled-content style="fixed-case">IL</jats:styled-content>‐1β (10 ng/<jats:styled-content style="fixed-case">mL</jats:styled-content> and 25 ng/<jats:styled-content style="fixed-case">mL</jats:styled-content>) increased the expression of <jats:styled-content style="fixed-case">MCP</jats:styled-content>‐1 <jats:styled-content style="fixed-case">mRNA</jats:styled-content> and protein in periodontal ligament cells (p < 0.05). In addition, the supernatants from the <jats:styled-content style="fixed-case">IL</jats:styled-content>‐1β‐treated periodontal ligament cells increased the migratory response of <jats:styled-content style="fixed-case">THP</jats:styled-content>‐1 cells (p < 0.05), which could be blocked by the anti‐<jats:styled-content style="fixed-case">MCP</jats:styled-content>‐1 antibody.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p><jats:styled-content style="fixed-case">IL</jats:styled-content>‐1β had the potential to induce the migratory response of monocytes via upregulation of the expression of <jats:styled-content style="fixed-case">MCP</jats:styled-content>‐1 in human periodontal ligament cells and could contribute to orthodontic root resorption.</jats:p></jats:sec> Upregulated expression of monocyte chemoattractant protein‐1 in human periodontal ligament cells induced by interleukin‐1β Australian Dental Journal |
| doi_str_mv |
10.1111/adj.12234 |
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Online Free |
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ElectronicArticle |
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| imprint |
Wiley, 2015 |
| imprint_str_mv |
Wiley, 2015 |
| issn |
1834-7819 0045-0421 |
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1834-7819 0045-0421 |
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jin2015upregulatedexpressionofmonocytechemoattractantprotein1inhumanperiodontalligamentcellsinducedbyinterleukin1b |
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2015 |
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Wiley |
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ai |
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ai |
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Australian Dental Journal |
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49 |
| title |
Upregulated expression of monocyte chemoattractant protein‐1 in human periodontal ligament cells induced by interleukin‐1β |
| title_unstemmed |
Upregulated expression of monocyte chemoattractant protein‐1 in human periodontal ligament cells induced by interleukin‐1β |
| title_full |
Upregulated expression of monocyte chemoattractant protein‐1 in human periodontal ligament cells induced by interleukin‐1β |
| title_fullStr |
Upregulated expression of monocyte chemoattractant protein‐1 in human periodontal ligament cells induced by interleukin‐1β |
| title_full_unstemmed |
Upregulated expression of monocyte chemoattractant protein‐1 in human periodontal ligament cells induced by interleukin‐1β |
| title_short |
Upregulated expression of monocyte chemoattractant protein‐1 in human periodontal ligament cells induced by interleukin‐1β |
| title_sort |
upregulated expression of monocyte chemoattractant protein‐1 in human periodontal ligament cells induced by interleukin‐1β |
| topic |
General Dentistry |
| url |
http://dx.doi.org/10.1111/adj.12234 |
| publishDate |
2015 |
| physical |
382-389 |
| description |
<jats:title>Abstract</jats:title><jats:sec><jats:title>Background</jats:title><jats:p>Root resorption during orthodontic treatment is a complex and sterile inflammatory process, characterized by the recruitment of mononuclear cells in the local periodontal ligament. This study aimed to investigate whether interleukin (<jats:styled-content style="fixed-case">IL</jats:styled-content>)‐1β could induce the migration of monocytes through upregulating monocyte chemoattractant protein (<jats:styled-content style="fixed-case">MCP</jats:styled-content>)‐1 expression in human periodontal ligament cells.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>Human periodontal ligament cells were cultured in medium containing various <jats:styled-content style="fixed-case">IL</jats:styled-content>‐1β concentrations. After 24 hours of incubation, the messenger <jats:styled-content style="fixed-case">RNA</jats:styled-content> (<jats:styled-content style="fixed-case">mRNA</jats:styled-content>) and <jats:styled-content style="fixed-case">MCP</jats:styled-content>‐1 protein in periodontal ligament cells were detected by reverse transcriptase–polymerase chain reaction and Western blot analysis, respectively. The effect of supernatants of periodontal ligament cells on <jats:styled-content style="fixed-case">THP</jats:styled-content>‐1 cells was analysed via migration assay.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p><jats:styled-content style="fixed-case">IL</jats:styled-content>‐1β (10 ng/<jats:styled-content style="fixed-case">mL</jats:styled-content> and 25 ng/<jats:styled-content style="fixed-case">mL</jats:styled-content>) increased the expression of <jats:styled-content style="fixed-case">MCP</jats:styled-content>‐1 <jats:styled-content style="fixed-case">mRNA</jats:styled-content> and protein in periodontal ligament cells (p < 0.05). In addition, the supernatants from the <jats:styled-content style="fixed-case">IL</jats:styled-content>‐1β‐treated periodontal ligament cells increased the migratory response of <jats:styled-content style="fixed-case">THP</jats:styled-content>‐1 cells (p < 0.05), which could be blocked by the anti‐<jats:styled-content style="fixed-case">MCP</jats:styled-content>‐1 antibody.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p><jats:styled-content style="fixed-case">IL</jats:styled-content>‐1β had the potential to induce the migratory response of monocytes via upregulation of the expression of <jats:styled-content style="fixed-case">MCP</jats:styled-content>‐1 in human periodontal ligament cells and could contribute to orthodontic root resorption.</jats:p></jats:sec> |
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| author | Jin, J, Cao, J |
| author_facet | Jin, J, Cao, J, Jin, J, Cao, J |
| author_sort | jin, j |
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| container_start_page | 382 |
| container_title | Australian Dental Journal |
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| description | <jats:title>Abstract</jats:title><jats:sec><jats:title>Background</jats:title><jats:p>Root resorption during orthodontic treatment is a complex and sterile inflammatory process, characterized by the recruitment of mononuclear cells in the local periodontal ligament. This study aimed to investigate whether interleukin (<jats:styled-content style="fixed-case">IL</jats:styled-content>)‐1β could induce the migration of monocytes through upregulating monocyte chemoattractant protein (<jats:styled-content style="fixed-case">MCP</jats:styled-content>)‐1 expression in human periodontal ligament cells.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>Human periodontal ligament cells were cultured in medium containing various <jats:styled-content style="fixed-case">IL</jats:styled-content>‐1β concentrations. After 24 hours of incubation, the messenger <jats:styled-content style="fixed-case">RNA</jats:styled-content> (<jats:styled-content style="fixed-case">mRNA</jats:styled-content>) and <jats:styled-content style="fixed-case">MCP</jats:styled-content>‐1 protein in periodontal ligament cells were detected by reverse transcriptase–polymerase chain reaction and Western blot analysis, respectively. The effect of supernatants of periodontal ligament cells on <jats:styled-content style="fixed-case">THP</jats:styled-content>‐1 cells was analysed via migration assay.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p><jats:styled-content style="fixed-case">IL</jats:styled-content>‐1β (10 ng/<jats:styled-content style="fixed-case">mL</jats:styled-content> and 25 ng/<jats:styled-content style="fixed-case">mL</jats:styled-content>) increased the expression of <jats:styled-content style="fixed-case">MCP</jats:styled-content>‐1 <jats:styled-content style="fixed-case">mRNA</jats:styled-content> and protein in periodontal ligament cells (p < 0.05). In addition, the supernatants from the <jats:styled-content style="fixed-case">IL</jats:styled-content>‐1β‐treated periodontal ligament cells increased the migratory response of <jats:styled-content style="fixed-case">THP</jats:styled-content>‐1 cells (p < 0.05), which could be blocked by the anti‐<jats:styled-content style="fixed-case">MCP</jats:styled-content>‐1 antibody.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p><jats:styled-content style="fixed-case">IL</jats:styled-content>‐1β had the potential to induce the migratory response of monocytes via upregulation of the expression of <jats:styled-content style="fixed-case">MCP</jats:styled-content>‐1 in human periodontal ligament cells and could contribute to orthodontic root resorption.</jats:p></jats:sec> |
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| imprint | Wiley, 2015 |
| imprint_str_mv | Wiley, 2015 |
| institution | DE-Gla1, DE-Zi4, DE-15, DE-Rs1, DE-Pl11, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161 |
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| physical | 382-389 |
| publishDate | 2015 |
| publishDateSort | 2015 |
| publisher | Wiley |
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| series | Australian Dental Journal |
| source_id | 49 |
| spelling | Jin, J Cao, J 0045-0421 1834-7819 Wiley General Dentistry http://dx.doi.org/10.1111/adj.12234 <jats:title>Abstract</jats:title><jats:sec><jats:title>Background</jats:title><jats:p>Root resorption during orthodontic treatment is a complex and sterile inflammatory process, characterized by the recruitment of mononuclear cells in the local periodontal ligament. This study aimed to investigate whether interleukin (<jats:styled-content style="fixed-case">IL</jats:styled-content>)‐1β could induce the migration of monocytes through upregulating monocyte chemoattractant protein (<jats:styled-content style="fixed-case">MCP</jats:styled-content>)‐1 expression in human periodontal ligament cells.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>Human periodontal ligament cells were cultured in medium containing various <jats:styled-content style="fixed-case">IL</jats:styled-content>‐1β concentrations. After 24 hours of incubation, the messenger <jats:styled-content style="fixed-case">RNA</jats:styled-content> (<jats:styled-content style="fixed-case">mRNA</jats:styled-content>) and <jats:styled-content style="fixed-case">MCP</jats:styled-content>‐1 protein in periodontal ligament cells were detected by reverse transcriptase–polymerase chain reaction and Western blot analysis, respectively. The effect of supernatants of periodontal ligament cells on <jats:styled-content style="fixed-case">THP</jats:styled-content>‐1 cells was analysed via migration assay.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p><jats:styled-content style="fixed-case">IL</jats:styled-content>‐1β (10 ng/<jats:styled-content style="fixed-case">mL</jats:styled-content> and 25 ng/<jats:styled-content style="fixed-case">mL</jats:styled-content>) increased the expression of <jats:styled-content style="fixed-case">MCP</jats:styled-content>‐1 <jats:styled-content style="fixed-case">mRNA</jats:styled-content> and protein in periodontal ligament cells (p < 0.05). In addition, the supernatants from the <jats:styled-content style="fixed-case">IL</jats:styled-content>‐1β‐treated periodontal ligament cells increased the migratory response of <jats:styled-content style="fixed-case">THP</jats:styled-content>‐1 cells (p < 0.05), which could be blocked by the anti‐<jats:styled-content style="fixed-case">MCP</jats:styled-content>‐1 antibody.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p><jats:styled-content style="fixed-case">IL</jats:styled-content>‐1β had the potential to induce the migratory response of monocytes via upregulation of the expression of <jats:styled-content style="fixed-case">MCP</jats:styled-content>‐1 in human periodontal ligament cells and could contribute to orthodontic root resorption.</jats:p></jats:sec> Upregulated expression of monocyte chemoattractant protein‐1 in human periodontal ligament cells induced by interleukin‐1β Australian Dental Journal |
| spellingShingle | Jin, J, Cao, J, Australian Dental Journal, Upregulated expression of monocyte chemoattractant protein‐1 in human periodontal ligament cells induced by interleukin‐1β, General Dentistry |
| title | Upregulated expression of monocyte chemoattractant protein‐1 in human periodontal ligament cells induced by interleukin‐1β |
| title_full | Upregulated expression of monocyte chemoattractant protein‐1 in human periodontal ligament cells induced by interleukin‐1β |
| title_fullStr | Upregulated expression of monocyte chemoattractant protein‐1 in human periodontal ligament cells induced by interleukin‐1β |
| title_full_unstemmed | Upregulated expression of monocyte chemoattractant protein‐1 in human periodontal ligament cells induced by interleukin‐1β |
| title_short | Upregulated expression of monocyte chemoattractant protein‐1 in human periodontal ligament cells induced by interleukin‐1β |
| title_sort | upregulated expression of monocyte chemoattractant protein‐1 in human periodontal ligament cells induced by interleukin‐1β |
| title_unstemmed | Upregulated expression of monocyte chemoattractant protein‐1 in human periodontal ligament cells induced by interleukin‐1β |
| topic | General Dentistry |
| url | http://dx.doi.org/10.1111/adj.12234 |