Eintrag weiter verarbeiten
Single-cell RNA-seq reveals changes in cell cycle and differentiation programs upon aging of hematopoietic stem cells
Gespeichert in:
| Zeitschriftentitel: | Genome Research |
|---|---|
| Personen und Körperschaften: | , , , , , , , , , |
| In: | Genome Research, 25, 2015, 12, S. 1860-1872 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Cold Spring Harbor Laboratory
|
| Schlagwörter: |
| author_facet |
Kowalczyk, Monika S. Tirosh, Itay Heckl, Dirk Rao, Tata Nageswara Dixit, Atray Haas, Brian J. Schneider, Rebekka K. Wagers, Amy J. Ebert, Benjamin L. Regev, Aviv Kowalczyk, Monika S. Tirosh, Itay Heckl, Dirk Rao, Tata Nageswara Dixit, Atray Haas, Brian J. Schneider, Rebekka K. Wagers, Amy J. Ebert, Benjamin L. Regev, Aviv |
|---|---|
| author |
Kowalczyk, Monika S. Tirosh, Itay Heckl, Dirk Rao, Tata Nageswara Dixit, Atray Haas, Brian J. Schneider, Rebekka K. Wagers, Amy J. Ebert, Benjamin L. Regev, Aviv |
| spellingShingle |
Kowalczyk, Monika S. Tirosh, Itay Heckl, Dirk Rao, Tata Nageswara Dixit, Atray Haas, Brian J. Schneider, Rebekka K. Wagers, Amy J. Ebert, Benjamin L. Regev, Aviv Genome Research Single-cell RNA-seq reveals changes in cell cycle and differentiation programs upon aging of hematopoietic stem cells Genetics (clinical) Genetics |
| author_sort |
kowalczyk, monika s. |
| spelling |
Kowalczyk, Monika S. Tirosh, Itay Heckl, Dirk Rao, Tata Nageswara Dixit, Atray Haas, Brian J. Schneider, Rebekka K. Wagers, Amy J. Ebert, Benjamin L. Regev, Aviv 1088-9051 1549-5469 Cold Spring Harbor Laboratory Genetics (clinical) Genetics http://dx.doi.org/10.1101/gr.192237.115 <jats:p>Both intrinsic cell state changes and variations in the composition of stem cell populations have been implicated as contributors to aging. We used single-cell RNA-seq to dissect variability in hematopoietic stem cell (HSC) and hematopoietic progenitor cell populations from young and old mice from two strains. We found that cell cycle dominates the variability within each population and that there is a lower frequency of cells in the G1 phase among old compared with young long-term HSCs, suggesting that they traverse through G1 faster. Moreover, transcriptional changes in HSCs during aging are inversely related to those upon HSC differentiation, such that old short-term (ST) HSCs resemble young long-term (LT-HSCs), suggesting that they exist in a less differentiated state. Our results indicate both compositional changes and intrinsic, population-wide changes with age and are consistent with a model where a relationship between cell cycle progression and self-renewal versus differentiation of HSCs is affected by aging and may contribute to the functional decline of old HSCs.</jats:p> Single-cell RNA-seq reveals changes in cell cycle and differentiation programs upon aging of hematopoietic stem cells Genome Research |
| doi_str_mv |
10.1101/gr.192237.115 |
| facet_avail |
Online Free |
| finc_class_facet |
Biologie |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTEwMS9nci4xOTIyMzcuMTE1 |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTEwMS9nci4xOTIyMzcuMTE1 |
| institution |
DE-D275 DE-Bn3 DE-Brt1 DE-Zwi2 DE-D161 DE-Zi4 DE-Gla1 DE-15 DE-Pl11 DE-Rs1 DE-14 DE-105 DE-Ch1 DE-L229 |
| imprint |
Cold Spring Harbor Laboratory, 2015 |
| imprint_str_mv |
Cold Spring Harbor Laboratory, 2015 |
| issn |
1088-9051 1549-5469 |
| issn_str_mv |
1088-9051 1549-5469 |
| language |
English |
| mega_collection |
Cold Spring Harbor Laboratory (CrossRef) |
| match_str |
kowalczyk2015singlecellrnaseqrevealschangesincellcycleanddifferentiationprogramsuponagingofhematopoieticstemcells |
| publishDateSort |
2015 |
| publisher |
Cold Spring Harbor Laboratory |
| recordtype |
ai |
| record_format |
ai |
| series |
Genome Research |
| source_id |
49 |
| title |
Single-cell RNA-seq reveals changes in cell cycle and differentiation programs upon aging of hematopoietic stem cells |
| title_unstemmed |
Single-cell RNA-seq reveals changes in cell cycle and differentiation programs upon aging of hematopoietic stem cells |
| title_full |
Single-cell RNA-seq reveals changes in cell cycle and differentiation programs upon aging of hematopoietic stem cells |
| title_fullStr |
Single-cell RNA-seq reveals changes in cell cycle and differentiation programs upon aging of hematopoietic stem cells |
| title_full_unstemmed |
Single-cell RNA-seq reveals changes in cell cycle and differentiation programs upon aging of hematopoietic stem cells |
| title_short |
Single-cell RNA-seq reveals changes in cell cycle and differentiation programs upon aging of hematopoietic stem cells |
| title_sort |
single-cell rna-seq reveals changes in cell cycle and differentiation programs upon aging of hematopoietic stem cells |
| topic |
Genetics (clinical) Genetics |
| url |
http://dx.doi.org/10.1101/gr.192237.115 |
| publishDate |
2015 |
| physical |
1860-1872 |
| description |
<jats:p>Both intrinsic cell state changes and variations in the composition of stem cell populations have been implicated as contributors to aging. We used single-cell RNA-seq to dissect variability in hematopoietic stem cell (HSC) and hematopoietic progenitor cell populations from young and old mice from two strains. We found that cell cycle dominates the variability within each population and that there is a lower frequency of cells in the G1 phase among old compared with young long-term HSCs, suggesting that they traverse through G1 faster. Moreover, transcriptional changes in HSCs during aging are inversely related to those upon HSC differentiation, such that old short-term (ST) HSCs resemble young long-term (LT-HSCs), suggesting that they exist in a less differentiated state. Our results indicate both compositional changes and intrinsic, population-wide changes with age and are consistent with a model where a relationship between cell cycle progression and self-renewal versus differentiation of HSCs is affected by aging and may contribute to the functional decline of old HSCs.</jats:p> |
| container_issue |
12 |
| container_start_page |
1860 |
| container_title |
Genome Research |
| container_volume |
25 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792344844676366346 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T17:13:42.425Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Single-cell+RNA-seq+reveals+changes+in+cell+cycle+and+differentiation+programs+upon+aging+of+hematopoietic+stem+cells&rft.date=2015-12-01&genre=article&issn=1549-5469&volume=25&issue=12&spage=1860&epage=1872&pages=1860-1872&jtitle=Genome+Research&atitle=Single-cell+RNA-seq+reveals+changes+in+cell+cycle+and+differentiation+programs+upon+aging+of+hematopoietic+stem+cells&aulast=Regev&aufirst=Aviv&rft_id=info%3Adoi%2F10.1101%2Fgr.192237.115&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792344844676366346 |
| author | Kowalczyk, Monika S., Tirosh, Itay, Heckl, Dirk, Rao, Tata Nageswara, Dixit, Atray, Haas, Brian J., Schneider, Rebekka K., Wagers, Amy J., Ebert, Benjamin L., Regev, Aviv |
| author_facet | Kowalczyk, Monika S., Tirosh, Itay, Heckl, Dirk, Rao, Tata Nageswara, Dixit, Atray, Haas, Brian J., Schneider, Rebekka K., Wagers, Amy J., Ebert, Benjamin L., Regev, Aviv, Kowalczyk, Monika S., Tirosh, Itay, Heckl, Dirk, Rao, Tata Nageswara, Dixit, Atray, Haas, Brian J., Schneider, Rebekka K., Wagers, Amy J., Ebert, Benjamin L., Regev, Aviv |
| author_sort | kowalczyk, monika s. |
| container_issue | 12 |
| container_start_page | 1860 |
| container_title | Genome Research |
| container_volume | 25 |
| description | <jats:p>Both intrinsic cell state changes and variations in the composition of stem cell populations have been implicated as contributors to aging. We used single-cell RNA-seq to dissect variability in hematopoietic stem cell (HSC) and hematopoietic progenitor cell populations from young and old mice from two strains. We found that cell cycle dominates the variability within each population and that there is a lower frequency of cells in the G1 phase among old compared with young long-term HSCs, suggesting that they traverse through G1 faster. Moreover, transcriptional changes in HSCs during aging are inversely related to those upon HSC differentiation, such that old short-term (ST) HSCs resemble young long-term (LT-HSCs), suggesting that they exist in a less differentiated state. Our results indicate both compositional changes and intrinsic, population-wide changes with age and are consistent with a model where a relationship between cell cycle progression and self-renewal versus differentiation of HSCs is affected by aging and may contribute to the functional decline of old HSCs.</jats:p> |
| doi_str_mv | 10.1101/gr.192237.115 |
| facet_avail | Online, Free |
| finc_class_facet | Biologie |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTEwMS9nci4xOTIyMzcuMTE1 |
| imprint | Cold Spring Harbor Laboratory, 2015 |
| imprint_str_mv | Cold Spring Harbor Laboratory, 2015 |
| institution | DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Zi4, DE-Gla1, DE-15, DE-Pl11, DE-Rs1, DE-14, DE-105, DE-Ch1, DE-L229 |
| issn | 1088-9051, 1549-5469 |
| issn_str_mv | 1088-9051, 1549-5469 |
| language | English |
| last_indexed | 2024-03-01T17:13:42.425Z |
| match_str | kowalczyk2015singlecellrnaseqrevealschangesincellcycleanddifferentiationprogramsuponagingofhematopoieticstemcells |
| mega_collection | Cold Spring Harbor Laboratory (CrossRef) |
| physical | 1860-1872 |
| publishDate | 2015 |
| publishDateSort | 2015 |
| publisher | Cold Spring Harbor Laboratory |
| record_format | ai |
| recordtype | ai |
| series | Genome Research |
| source_id | 49 |
| spelling | Kowalczyk, Monika S. Tirosh, Itay Heckl, Dirk Rao, Tata Nageswara Dixit, Atray Haas, Brian J. Schneider, Rebekka K. Wagers, Amy J. Ebert, Benjamin L. Regev, Aviv 1088-9051 1549-5469 Cold Spring Harbor Laboratory Genetics (clinical) Genetics http://dx.doi.org/10.1101/gr.192237.115 <jats:p>Both intrinsic cell state changes and variations in the composition of stem cell populations have been implicated as contributors to aging. We used single-cell RNA-seq to dissect variability in hematopoietic stem cell (HSC) and hematopoietic progenitor cell populations from young and old mice from two strains. We found that cell cycle dominates the variability within each population and that there is a lower frequency of cells in the G1 phase among old compared with young long-term HSCs, suggesting that they traverse through G1 faster. Moreover, transcriptional changes in HSCs during aging are inversely related to those upon HSC differentiation, such that old short-term (ST) HSCs resemble young long-term (LT-HSCs), suggesting that they exist in a less differentiated state. Our results indicate both compositional changes and intrinsic, population-wide changes with age and are consistent with a model where a relationship between cell cycle progression and self-renewal versus differentiation of HSCs is affected by aging and may contribute to the functional decline of old HSCs.</jats:p> Single-cell RNA-seq reveals changes in cell cycle and differentiation programs upon aging of hematopoietic stem cells Genome Research |
| spellingShingle | Kowalczyk, Monika S., Tirosh, Itay, Heckl, Dirk, Rao, Tata Nageswara, Dixit, Atray, Haas, Brian J., Schneider, Rebekka K., Wagers, Amy J., Ebert, Benjamin L., Regev, Aviv, Genome Research, Single-cell RNA-seq reveals changes in cell cycle and differentiation programs upon aging of hematopoietic stem cells, Genetics (clinical), Genetics |
| title | Single-cell RNA-seq reveals changes in cell cycle and differentiation programs upon aging of hematopoietic stem cells |
| title_full | Single-cell RNA-seq reveals changes in cell cycle and differentiation programs upon aging of hematopoietic stem cells |
| title_fullStr | Single-cell RNA-seq reveals changes in cell cycle and differentiation programs upon aging of hematopoietic stem cells |
| title_full_unstemmed | Single-cell RNA-seq reveals changes in cell cycle and differentiation programs upon aging of hematopoietic stem cells |
| title_short | Single-cell RNA-seq reveals changes in cell cycle and differentiation programs upon aging of hematopoietic stem cells |
| title_sort | single-cell rna-seq reveals changes in cell cycle and differentiation programs upon aging of hematopoietic stem cells |
| title_unstemmed | Single-cell RNA-seq reveals changes in cell cycle and differentiation programs upon aging of hematopoietic stem cells |
| topic | Genetics (clinical), Genetics |
| url | http://dx.doi.org/10.1101/gr.192237.115 |