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Saccharomyces forkhead protein Fkh1 regulates donor preference during mating-type switching through the recombination enhancer
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| Zeitschriftentitel: | Genes & Development |
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| Personen und Körperschaften: | , , , , |
| In: | Genes & Development, 16, 2002, 16, S. 2085-2096 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Cold Spring Harbor Laboratory
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| Schlagwörter: |
| author_facet |
Sun, Kaiming Coı̈c, Eric Zhou, Zhiqi Durrens, Pascal Haber, James E. Sun, Kaiming Coı̈c, Eric Zhou, Zhiqi Durrens, Pascal Haber, James E. |
|---|---|
| author |
Sun, Kaiming Coı̈c, Eric Zhou, Zhiqi Durrens, Pascal Haber, James E. |
| spellingShingle |
Sun, Kaiming Coı̈c, Eric Zhou, Zhiqi Durrens, Pascal Haber, James E. Genes & Development Saccharomyces forkhead protein Fkh1 regulates donor preference during mating-type switching through the recombination enhancer Developmental Biology Genetics |
| author_sort |
sun, kaiming |
| spelling |
Sun, Kaiming Coı̈c, Eric Zhou, Zhiqi Durrens, Pascal Haber, James E. 0890-9369 1549-5477 Cold Spring Harbor Laboratory Developmental Biology Genetics http://dx.doi.org/10.1101/gad.994902 <jats:p><jats:italic>Saccharomyces</jats:italic> mating-type switching results from replacement by gene conversion of the <jats:italic>MAT</jats:italic> locus with sequences copied from one of two unexpressed donor loci, <jats:italic>HML</jats:italic> or <jats:italic>HMR. MAT</jats:italic>a cells recombine with <jats:italic>HML</jats:italic>α ∼90% of the time, whereas <jats:italic>MAT</jats:italic>α cells choose <jats:italic>HMR</jats:italic>a 80%–90% of the time. <jats:italic>HML</jats:italic> preference in <jats:italic>MAT</jats:italic>a is controlled by the cis-acting recombination enhancer (RE) that regulates recombination along the entire left arm of chromosome III. Comparison of RE sequences between <jats:italic>S. cerevisiae</jats:italic>, <jats:italic>S. carlsbergensis</jats:italic>, and <jats:italic>S. bayanus</jats:italic> defines four highly conserved regions (A, B, C, and D) within a 270-bp minimum RE. An adjacent E region enhances RE activity. Multimers of region A, D, or E are sufficient to promote selective use of <jats:italic>HML</jats:italic>. Regions A, D, and E each bind in vivo the transcription activator forkhead proteins Fkh1p and Fkh2p and their associated Ndd1p, although there are no adjacent open reading frames (ORFs). Deletion of <jats:italic>FKH1</jats:italic>significantly reduces <jats:italic>MAT</jats:italic>a's use of <jats:italic>HML</jats:italic>, as does mutation of the Fkh1/Fkh2-binding sites in a multimer of region A. We conclude that Fkh1p regulates <jats:italic>MAT</jats:italic>a donor preference through direct interaction with RE.</jats:p> <i>Saccharomyces</i> forkhead protein Fkh1 regulates donor preference during mating-type switching through the recombination enhancer Genes & Development |
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10.1101/gad.994902 |
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Cold Spring Harbor Laboratory, 2002 |
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| title |
Saccharomyces forkhead protein Fkh1 regulates donor preference during mating-type switching through the recombination enhancer |
| title_unstemmed |
Saccharomyces forkhead protein Fkh1 regulates donor preference during mating-type switching through the recombination enhancer |
| title_full |
Saccharomyces forkhead protein Fkh1 regulates donor preference during mating-type switching through the recombination enhancer |
| title_fullStr |
Saccharomyces forkhead protein Fkh1 regulates donor preference during mating-type switching through the recombination enhancer |
| title_full_unstemmed |
Saccharomyces forkhead protein Fkh1 regulates donor preference during mating-type switching through the recombination enhancer |
| title_short |
Saccharomyces forkhead protein Fkh1 regulates donor preference during mating-type switching through the recombination enhancer |
| title_sort |
<i>saccharomyces</i> forkhead protein fkh1 regulates donor preference during mating-type switching through the recombination enhancer |
| topic |
Developmental Biology Genetics |
| url |
http://dx.doi.org/10.1101/gad.994902 |
| publishDate |
2002 |
| physical |
2085-2096 |
| description |
<jats:p><jats:italic>Saccharomyces</jats:italic> mating-type switching results from replacement by gene conversion of the <jats:italic>MAT</jats:italic> locus with sequences copied from one of two unexpressed donor loci, <jats:italic>HML</jats:italic> or <jats:italic>HMR. MAT</jats:italic>a cells recombine with <jats:italic>HML</jats:italic>α ∼90% of the time, whereas <jats:italic>MAT</jats:italic>α cells choose <jats:italic>HMR</jats:italic>a 80%–90% of the time. <jats:italic>HML</jats:italic> preference in <jats:italic>MAT</jats:italic>a is controlled by the cis-acting recombination enhancer (RE) that regulates recombination along the entire left arm of chromosome III. Comparison of RE sequences between <jats:italic>S. cerevisiae</jats:italic>, <jats:italic>S. carlsbergensis</jats:italic>, and <jats:italic>S. bayanus</jats:italic> defines four highly conserved regions (A, B, C, and D) within a 270-bp minimum RE. An adjacent E region enhances RE activity. Multimers of region A, D, or E are sufficient to promote selective use of <jats:italic>HML</jats:italic>. Regions A, D, and E each bind in vivo the transcription activator forkhead proteins Fkh1p and Fkh2p and their associated Ndd1p, although there are no adjacent open reading frames (ORFs). Deletion of <jats:italic>FKH1</jats:italic>significantly reduces <jats:italic>MAT</jats:italic>a's use of <jats:italic>HML</jats:italic>, as does mutation of the Fkh1/Fkh2-binding sites in a multimer of region A. We conclude that Fkh1p regulates <jats:italic>MAT</jats:italic>a donor preference through direct interaction with RE.</jats:p> |
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| author | Sun, Kaiming, Coı̈c, Eric, Zhou, Zhiqi, Durrens, Pascal, Haber, James E. |
| author_facet | Sun, Kaiming, Coı̈c, Eric, Zhou, Zhiqi, Durrens, Pascal, Haber, James E., Sun, Kaiming, Coı̈c, Eric, Zhou, Zhiqi, Durrens, Pascal, Haber, James E. |
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| description | <jats:p><jats:italic>Saccharomyces</jats:italic> mating-type switching results from replacement by gene conversion of the <jats:italic>MAT</jats:italic> locus with sequences copied from one of two unexpressed donor loci, <jats:italic>HML</jats:italic> or <jats:italic>HMR. MAT</jats:italic>a cells recombine with <jats:italic>HML</jats:italic>α ∼90% of the time, whereas <jats:italic>MAT</jats:italic>α cells choose <jats:italic>HMR</jats:italic>a 80%–90% of the time. <jats:italic>HML</jats:italic> preference in <jats:italic>MAT</jats:italic>a is controlled by the cis-acting recombination enhancer (RE) that regulates recombination along the entire left arm of chromosome III. Comparison of RE sequences between <jats:italic>S. cerevisiae</jats:italic>, <jats:italic>S. carlsbergensis</jats:italic>, and <jats:italic>S. bayanus</jats:italic> defines four highly conserved regions (A, B, C, and D) within a 270-bp minimum RE. An adjacent E region enhances RE activity. Multimers of region A, D, or E are sufficient to promote selective use of <jats:italic>HML</jats:italic>. Regions A, D, and E each bind in vivo the transcription activator forkhead proteins Fkh1p and Fkh2p and their associated Ndd1p, although there are no adjacent open reading frames (ORFs). Deletion of <jats:italic>FKH1</jats:italic>significantly reduces <jats:italic>MAT</jats:italic>a's use of <jats:italic>HML</jats:italic>, as does mutation of the Fkh1/Fkh2-binding sites in a multimer of region A. We conclude that Fkh1p regulates <jats:italic>MAT</jats:italic>a donor preference through direct interaction with RE.</jats:p> |
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| imprint_str_mv | Cold Spring Harbor Laboratory, 2002 |
| institution | DE-14, DE-105, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Zi4, DE-Gla1, DE-15, DE-Pl11, DE-Rs1 |
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| physical | 2085-2096 |
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| publisher | Cold Spring Harbor Laboratory |
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| series | Genes & Development |
| source_id | 49 |
| spelling | Sun, Kaiming Coı̈c, Eric Zhou, Zhiqi Durrens, Pascal Haber, James E. 0890-9369 1549-5477 Cold Spring Harbor Laboratory Developmental Biology Genetics http://dx.doi.org/10.1101/gad.994902 <jats:p><jats:italic>Saccharomyces</jats:italic> mating-type switching results from replacement by gene conversion of the <jats:italic>MAT</jats:italic> locus with sequences copied from one of two unexpressed donor loci, <jats:italic>HML</jats:italic> or <jats:italic>HMR. MAT</jats:italic>a cells recombine with <jats:italic>HML</jats:italic>α ∼90% of the time, whereas <jats:italic>MAT</jats:italic>α cells choose <jats:italic>HMR</jats:italic>a 80%–90% of the time. <jats:italic>HML</jats:italic> preference in <jats:italic>MAT</jats:italic>a is controlled by the cis-acting recombination enhancer (RE) that regulates recombination along the entire left arm of chromosome III. Comparison of RE sequences between <jats:italic>S. cerevisiae</jats:italic>, <jats:italic>S. carlsbergensis</jats:italic>, and <jats:italic>S. bayanus</jats:italic> defines four highly conserved regions (A, B, C, and D) within a 270-bp minimum RE. An adjacent E region enhances RE activity. Multimers of region A, D, or E are sufficient to promote selective use of <jats:italic>HML</jats:italic>. Regions A, D, and E each bind in vivo the transcription activator forkhead proteins Fkh1p and Fkh2p and their associated Ndd1p, although there are no adjacent open reading frames (ORFs). Deletion of <jats:italic>FKH1</jats:italic>significantly reduces <jats:italic>MAT</jats:italic>a's use of <jats:italic>HML</jats:italic>, as does mutation of the Fkh1/Fkh2-binding sites in a multimer of region A. We conclude that Fkh1p regulates <jats:italic>MAT</jats:italic>a donor preference through direct interaction with RE.</jats:p> <i>Saccharomyces</i> forkhead protein Fkh1 regulates donor preference during mating-type switching through the recombination enhancer Genes & Development |
| spellingShingle | Sun, Kaiming, Coı̈c, Eric, Zhou, Zhiqi, Durrens, Pascal, Haber, James E., Genes & Development, Saccharomyces forkhead protein Fkh1 regulates donor preference during mating-type switching through the recombination enhancer, Developmental Biology, Genetics |
| title | Saccharomyces forkhead protein Fkh1 regulates donor preference during mating-type switching through the recombination enhancer |
| title_full | Saccharomyces forkhead protein Fkh1 regulates donor preference during mating-type switching through the recombination enhancer |
| title_fullStr | Saccharomyces forkhead protein Fkh1 regulates donor preference during mating-type switching through the recombination enhancer |
| title_full_unstemmed | Saccharomyces forkhead protein Fkh1 regulates donor preference during mating-type switching through the recombination enhancer |
| title_short | Saccharomyces forkhead protein Fkh1 regulates donor preference during mating-type switching through the recombination enhancer |
| title_sort | <i>saccharomyces</i> forkhead protein fkh1 regulates donor preference during mating-type switching through the recombination enhancer |
| title_unstemmed | Saccharomyces forkhead protein Fkh1 regulates donor preference during mating-type switching through the recombination enhancer |
| topic | Developmental Biology, Genetics |
| url | http://dx.doi.org/10.1101/gad.994902 |