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A model for the abrogation of the SOS response by an SOS protein: a negatively charged helix in DinI mimics DNA in its interaction with RecA
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| Zeitschriftentitel: | Genes & Development |
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| Personen und Körperschaften: | , , , |
| In: | Genes & Development, 15, 2001, 4, S. 415-427 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Cold Spring Harbor Laboratory
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| Schlagwörter: |
| Zusammenfassung: | <jats:p>DinI is a recently described negative regulator of the SOS response in <jats:italic>Escherichia coli</jats:italic>. Here we show that it physically interacts with RecA and prevents the binding of single-stranded DNA to RecA, which is required for the activation of the latter. DinI also displaces ssDNA from a stable RecA–DNA cofilament, thus eliminating the SOS signal. In addition, DinI inhibits RecA-mediated homologous DNA pairing, but has no effect on actively proceeding strand exchange. Biochemical data, together with the molecular structure, define the C-terminal α-helix in DinI as the active site of the protein. In an unusual example of molecular mimicry, a negatively charged surface on this α-helix, by imitating single-stranded DNA, interacts with the loop L2 homologous pairing region of RecA and interferes with the activation of RecA.</jats:p> |
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| Umfang: | 415-427 |
| ISSN: |
0890-9369
1549-5477 |
| DOI: | 10.1101/gad.862901 |