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Interactions with Costal2 and Suppressor of fused regulate nuclear translocation and activity of Cubitus interruptus
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| Zeitschriftentitel: | Genes & Development |
|---|---|
| Personen und Körperschaften: | , , , |
| In: | Genes & Development, 14, 2000, 22, S. 2893-2905 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Cold Spring Harbor Laboratory
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| Schlagwörter: |
| author_facet |
Wang, Gelin Amanai, Kazuhito Wang, Bing Jiang, Jin Wang, Gelin Amanai, Kazuhito Wang, Bing Jiang, Jin |
|---|---|
| author |
Wang, Gelin Amanai, Kazuhito Wang, Bing Jiang, Jin |
| spellingShingle |
Wang, Gelin Amanai, Kazuhito Wang, Bing Jiang, Jin Genes & Development Interactions with Costal2 and Suppressor of fused regulate nuclear translocation and activity of Cubitus interruptus Developmental Biology Genetics |
| author_sort |
wang, gelin |
| spelling |
Wang, Gelin Amanai, Kazuhito Wang, Bing Jiang, Jin 0890-9369 1549-5477 Cold Spring Harbor Laboratory Developmental Biology Genetics http://dx.doi.org/10.1101/gad.843900 <jats:p>The Hedgehog (Hh) family of secreted proteins controls many aspects of growth and patterning in animal development. In<jats:italic>Drosophila,</jats:italic> Hh acts by preventing the formation of a truncated repressor form of Cubitus interruptus (Ci) and stimulating the transcriptional activity of full-length Ci. Here, we provide evidence that Costal2 (Cos2) and Suppressor of Fused [Su(fu)] inhibit Ci by tethering it in the cytoplasm, whereas Hh induces nuclear translocaltion of Ci through Fused (Fu). We have identified a 125 amino acid domain in the C-terminal part of Ci that mediates response to Cos2 inhibition. We show that Cos2 binds Ci, prevents its nuclear import, and inhibits its activity via this domain. We also provide evidence that Su(fu) regulates Ci through two distinct mechanisms: (1) Su(fu) blocks Ci nuclear import through the N-terminal region of Ci , and (2) it inhibits the activity of Ci through a mechanism independent of Ci nuclear translocation. Finally, we show that Cos2 is required for transducing high levels of Hh signaling activity, and it does so by alleviating the blockage of Ci activity imposed by Su(fu).</jats:p> Interactions with Costal2 and Suppressor of fused regulate nuclear translocation and activity of Cubitus interruptus Genes & Development |
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| title |
Interactions with Costal2 and Suppressor of fused regulate nuclear translocation and activity of Cubitus interruptus |
| title_unstemmed |
Interactions with Costal2 and Suppressor of fused regulate nuclear translocation and activity of Cubitus interruptus |
| title_full |
Interactions with Costal2 and Suppressor of fused regulate nuclear translocation and activity of Cubitus interruptus |
| title_fullStr |
Interactions with Costal2 and Suppressor of fused regulate nuclear translocation and activity of Cubitus interruptus |
| title_full_unstemmed |
Interactions with Costal2 and Suppressor of fused regulate nuclear translocation and activity of Cubitus interruptus |
| title_short |
Interactions with Costal2 and Suppressor of fused regulate nuclear translocation and activity of Cubitus interruptus |
| title_sort |
interactions with costal2 and suppressor of fused regulate nuclear translocation and activity of cubitus interruptus |
| topic |
Developmental Biology Genetics |
| url |
http://dx.doi.org/10.1101/gad.843900 |
| publishDate |
2000 |
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2893-2905 |
| description |
<jats:p>The Hedgehog (Hh) family of secreted proteins controls many aspects of growth and patterning in animal development. In<jats:italic>Drosophila,</jats:italic> Hh acts by preventing the formation of a truncated repressor form of Cubitus interruptus (Ci) and stimulating the transcriptional activity of full-length Ci. Here, we provide evidence that Costal2 (Cos2) and Suppressor of Fused [Su(fu)] inhibit Ci by tethering it in the cytoplasm, whereas Hh induces nuclear translocaltion of Ci through Fused (Fu). We have identified a 125 amino acid domain in the C-terminal part of Ci that mediates response to Cos2 inhibition. We show that Cos2 binds Ci, prevents its nuclear import, and inhibits its activity via this domain. We also provide evidence that Su(fu) regulates Ci through two distinct mechanisms: (1) Su(fu) blocks Ci nuclear import through the N-terminal region of Ci , and (2) it inhibits the activity of Ci through a mechanism independent of Ci nuclear translocation. Finally, we show that Cos2 is required for transducing high levels of Hh signaling activity, and it does so by alleviating the blockage of Ci activity imposed by Su(fu).</jats:p> |
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| author | Wang, Gelin, Amanai, Kazuhito, Wang, Bing, Jiang, Jin |
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| description | <jats:p>The Hedgehog (Hh) family of secreted proteins controls many aspects of growth and patterning in animal development. In<jats:italic>Drosophila,</jats:italic> Hh acts by preventing the formation of a truncated repressor form of Cubitus interruptus (Ci) and stimulating the transcriptional activity of full-length Ci. Here, we provide evidence that Costal2 (Cos2) and Suppressor of Fused [Su(fu)] inhibit Ci by tethering it in the cytoplasm, whereas Hh induces nuclear translocaltion of Ci through Fused (Fu). We have identified a 125 amino acid domain in the C-terminal part of Ci that mediates response to Cos2 inhibition. We show that Cos2 binds Ci, prevents its nuclear import, and inhibits its activity via this domain. We also provide evidence that Su(fu) regulates Ci through two distinct mechanisms: (1) Su(fu) blocks Ci nuclear import through the N-terminal region of Ci , and (2) it inhibits the activity of Ci through a mechanism independent of Ci nuclear translocation. Finally, we show that Cos2 is required for transducing high levels of Hh signaling activity, and it does so by alleviating the blockage of Ci activity imposed by Su(fu).</jats:p> |
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| imprint | Cold Spring Harbor Laboratory, 2000 |
| imprint_str_mv | Cold Spring Harbor Laboratory, 2000 |
| institution | DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-D161, DE-Zwi2, DE-Gla1, DE-Zi4 |
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| spelling | Wang, Gelin Amanai, Kazuhito Wang, Bing Jiang, Jin 0890-9369 1549-5477 Cold Spring Harbor Laboratory Developmental Biology Genetics http://dx.doi.org/10.1101/gad.843900 <jats:p>The Hedgehog (Hh) family of secreted proteins controls many aspects of growth and patterning in animal development. In<jats:italic>Drosophila,</jats:italic> Hh acts by preventing the formation of a truncated repressor form of Cubitus interruptus (Ci) and stimulating the transcriptional activity of full-length Ci. Here, we provide evidence that Costal2 (Cos2) and Suppressor of Fused [Su(fu)] inhibit Ci by tethering it in the cytoplasm, whereas Hh induces nuclear translocaltion of Ci through Fused (Fu). We have identified a 125 amino acid domain in the C-terminal part of Ci that mediates response to Cos2 inhibition. We show that Cos2 binds Ci, prevents its nuclear import, and inhibits its activity via this domain. We also provide evidence that Su(fu) regulates Ci through two distinct mechanisms: (1) Su(fu) blocks Ci nuclear import through the N-terminal region of Ci , and (2) it inhibits the activity of Ci through a mechanism independent of Ci nuclear translocation. Finally, we show that Cos2 is required for transducing high levels of Hh signaling activity, and it does so by alleviating the blockage of Ci activity imposed by Su(fu).</jats:p> Interactions with Costal2 and Suppressor of fused regulate nuclear translocation and activity of Cubitus interruptus Genes & Development |
| spellingShingle | Wang, Gelin, Amanai, Kazuhito, Wang, Bing, Jiang, Jin, Genes & Development, Interactions with Costal2 and Suppressor of fused regulate nuclear translocation and activity of Cubitus interruptus, Developmental Biology, Genetics |
| title | Interactions with Costal2 and Suppressor of fused regulate nuclear translocation and activity of Cubitus interruptus |
| title_full | Interactions with Costal2 and Suppressor of fused regulate nuclear translocation and activity of Cubitus interruptus |
| title_fullStr | Interactions with Costal2 and Suppressor of fused regulate nuclear translocation and activity of Cubitus interruptus |
| title_full_unstemmed | Interactions with Costal2 and Suppressor of fused regulate nuclear translocation and activity of Cubitus interruptus |
| title_short | Interactions with Costal2 and Suppressor of fused regulate nuclear translocation and activity of Cubitus interruptus |
| title_sort | interactions with costal2 and suppressor of fused regulate nuclear translocation and activity of cubitus interruptus |
| title_unstemmed | Interactions with Costal2 and Suppressor of fused regulate nuclear translocation and activity of Cubitus interruptus |
| topic | Developmental Biology, Genetics |
| url | http://dx.doi.org/10.1101/gad.843900 |