Eintrag weiter verarbeiten
A temperature-sensitive MEK mutation demonstrates the conservation of the signaling pathways activated by receptor tyrosine kinases.
Gespeichert in:
| Zeitschriftentitel: | Genes & Development |
|---|---|
| Personen und Körperschaften: | , |
| In: | Genes & Development, 8, 1994, 18, S. 2176-2187 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Cold Spring Harbor Laboratory
|
| Schlagwörter: |
| author_facet |
Hsu, J C Perrimon, N Hsu, J C Perrimon, N |
|---|---|
| author |
Hsu, J C Perrimon, N |
| spellingShingle |
Hsu, J C Perrimon, N Genes & Development A temperature-sensitive MEK mutation demonstrates the conservation of the signaling pathways activated by receptor tyrosine kinases. Developmental Biology Genetics |
| author_sort |
hsu, j c |
| spelling |
Hsu, J C Perrimon, N 0890-9369 1549-5477 Cold Spring Harbor Laboratory Developmental Biology Genetics http://dx.doi.org/10.1101/gad.8.18.2176 <jats:p>MEK, a dual specificity threonine/tyrosine kinase, has been postulated to be a convergent point for signaling from receptor protein tyrosine kinases (RTKs) and G-protein-coupled receptors. In contrast to yeast and mammalian cells where several MEKs have been isolated, only one Drosophila MEK (D-Mek) has been characterized to date. Previous studies have shown that D-Mek acts in the Torso RTK signaling pathway. To demonstrate that D-Mek also operates downstream of other RTKs, we generated a temperature-sensitive allele of D-mek (D-mekts) by site-directed mutagenesis based on the amino acid change of a yeast cdc2ts mutation. Using D-mekts, we show that in addition to its role in Torso signaling, D-Mek operates in the Sevenless and in the Drosophila epidermal growth factor RTK pathways. Because loss-of-function mutations in D-mek and the upstream receptors give rise to similar phenotypes, it suggests that D-mek is the only MEK activated by Drosophila RTKs. In addition, we demonstrate that different RTK pathways respond differently to alteration in D-Mek activity.</jats:p> A temperature-sensitive MEK mutation demonstrates the conservation of the signaling pathways activated by receptor tyrosine kinases. Genes & Development |
| doi_str_mv |
10.1101/gad.8.18.2176 |
| facet_avail |
Online Free |
| finc_class_facet |
Biologie |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTEwMS9nYWQuOC4xOC4yMTc2 |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTEwMS9nYWQuOC4xOC4yMTc2 |
| institution |
DE-L229 DE-D275 DE-Bn3 DE-Brt1 DE-D161 DE-Zwi2 DE-Gla1 DE-Zi4 DE-15 DE-Pl11 DE-Rs1 DE-105 DE-14 DE-Ch1 |
| imprint |
Cold Spring Harbor Laboratory, 1994 |
| imprint_str_mv |
Cold Spring Harbor Laboratory, 1994 |
| issn |
0890-9369 1549-5477 |
| issn_str_mv |
0890-9369 1549-5477 |
| language |
English |
| mega_collection |
Cold Spring Harbor Laboratory (CrossRef) |
| match_str |
hsu1994atemperaturesensitivemekmutationdemonstratestheconservationofthesignalingpathwaysactivatedbyreceptortyrosinekinases |
| publishDateSort |
1994 |
| publisher |
Cold Spring Harbor Laboratory |
| recordtype |
ai |
| record_format |
ai |
| series |
Genes & Development |
| source_id |
49 |
| title |
A temperature-sensitive MEK mutation demonstrates the conservation of the signaling pathways activated by receptor tyrosine kinases. |
| title_unstemmed |
A temperature-sensitive MEK mutation demonstrates the conservation of the signaling pathways activated by receptor tyrosine kinases. |
| title_full |
A temperature-sensitive MEK mutation demonstrates the conservation of the signaling pathways activated by receptor tyrosine kinases. |
| title_fullStr |
A temperature-sensitive MEK mutation demonstrates the conservation of the signaling pathways activated by receptor tyrosine kinases. |
| title_full_unstemmed |
A temperature-sensitive MEK mutation demonstrates the conservation of the signaling pathways activated by receptor tyrosine kinases. |
| title_short |
A temperature-sensitive MEK mutation demonstrates the conservation of the signaling pathways activated by receptor tyrosine kinases. |
| title_sort |
a temperature-sensitive mek mutation demonstrates the conservation of the signaling pathways activated by receptor tyrosine kinases. |
| topic |
Developmental Biology Genetics |
| url |
http://dx.doi.org/10.1101/gad.8.18.2176 |
| publishDate |
1994 |
| physical |
2176-2187 |
| description |
<jats:p>MEK, a dual specificity threonine/tyrosine kinase, has been postulated to be a convergent point for signaling from receptor protein tyrosine kinases (RTKs) and G-protein-coupled receptors. In contrast to yeast and mammalian cells where several MEKs have been isolated, only one Drosophila MEK (D-Mek) has been characterized to date. Previous studies have shown that D-Mek acts in the Torso RTK signaling pathway. To demonstrate that D-Mek also operates downstream of other RTKs, we generated a temperature-sensitive allele of D-mek (D-mekts) by site-directed mutagenesis based on the amino acid change of a yeast cdc2ts mutation. Using D-mekts, we show that in addition to its role in Torso signaling, D-Mek operates in the Sevenless and in the Drosophila epidermal growth factor RTK pathways. Because loss-of-function mutations in D-mek and the upstream receptors give rise to similar phenotypes, it suggests that D-mek is the only MEK activated by Drosophila RTKs. In addition, we demonstrate that different RTK pathways respond differently to alteration in D-Mek activity.</jats:p> |
| container_issue |
18 |
| container_start_page |
2176 |
| container_title |
Genes & Development |
| container_volume |
8 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792347085660487691 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T17:49:21.194Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=A+temperature-sensitive+MEK+mutation+demonstrates+the+conservation+of+the+signaling+pathways+activated+by+receptor+tyrosine+kinases.&rft.date=1994-09-15&genre=article&issn=1549-5477&volume=8&issue=18&spage=2176&epage=2187&pages=2176-2187&jtitle=Genes+%26+Development&atitle=A+temperature-sensitive+MEK+mutation+demonstrates+the+conservation+of+the+signaling+pathways+activated+by+receptor+tyrosine+kinases.&aulast=Perrimon&aufirst=N&rft_id=info%3Adoi%2F10.1101%2Fgad.8.18.2176&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792347085660487691 |
| author | Hsu, J C, Perrimon, N |
| author_facet | Hsu, J C, Perrimon, N, Hsu, J C, Perrimon, N |
| author_sort | hsu, j c |
| container_issue | 18 |
| container_start_page | 2176 |
| container_title | Genes & Development |
| container_volume | 8 |
| description | <jats:p>MEK, a dual specificity threonine/tyrosine kinase, has been postulated to be a convergent point for signaling from receptor protein tyrosine kinases (RTKs) and G-protein-coupled receptors. In contrast to yeast and mammalian cells where several MEKs have been isolated, only one Drosophila MEK (D-Mek) has been characterized to date. Previous studies have shown that D-Mek acts in the Torso RTK signaling pathway. To demonstrate that D-Mek also operates downstream of other RTKs, we generated a temperature-sensitive allele of D-mek (D-mekts) by site-directed mutagenesis based on the amino acid change of a yeast cdc2ts mutation. Using D-mekts, we show that in addition to its role in Torso signaling, D-Mek operates in the Sevenless and in the Drosophila epidermal growth factor RTK pathways. Because loss-of-function mutations in D-mek and the upstream receptors give rise to similar phenotypes, it suggests that D-mek is the only MEK activated by Drosophila RTKs. In addition, we demonstrate that different RTK pathways respond differently to alteration in D-Mek activity.</jats:p> |
| doi_str_mv | 10.1101/gad.8.18.2176 |
| facet_avail | Online, Free |
| finc_class_facet | Biologie |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTEwMS9nYWQuOC4xOC4yMTc2 |
| imprint | Cold Spring Harbor Laboratory, 1994 |
| imprint_str_mv | Cold Spring Harbor Laboratory, 1994 |
| institution | DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-D161, DE-Zwi2, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1 |
| issn | 0890-9369, 1549-5477 |
| issn_str_mv | 0890-9369, 1549-5477 |
| language | English |
| last_indexed | 2024-03-01T17:49:21.194Z |
| match_str | hsu1994atemperaturesensitivemekmutationdemonstratestheconservationofthesignalingpathwaysactivatedbyreceptortyrosinekinases |
| mega_collection | Cold Spring Harbor Laboratory (CrossRef) |
| physical | 2176-2187 |
| publishDate | 1994 |
| publishDateSort | 1994 |
| publisher | Cold Spring Harbor Laboratory |
| record_format | ai |
| recordtype | ai |
| series | Genes & Development |
| source_id | 49 |
| spelling | Hsu, J C Perrimon, N 0890-9369 1549-5477 Cold Spring Harbor Laboratory Developmental Biology Genetics http://dx.doi.org/10.1101/gad.8.18.2176 <jats:p>MEK, a dual specificity threonine/tyrosine kinase, has been postulated to be a convergent point for signaling from receptor protein tyrosine kinases (RTKs) and G-protein-coupled receptors. In contrast to yeast and mammalian cells where several MEKs have been isolated, only one Drosophila MEK (D-Mek) has been characterized to date. Previous studies have shown that D-Mek acts in the Torso RTK signaling pathway. To demonstrate that D-Mek also operates downstream of other RTKs, we generated a temperature-sensitive allele of D-mek (D-mekts) by site-directed mutagenesis based on the amino acid change of a yeast cdc2ts mutation. Using D-mekts, we show that in addition to its role in Torso signaling, D-Mek operates in the Sevenless and in the Drosophila epidermal growth factor RTK pathways. Because loss-of-function mutations in D-mek and the upstream receptors give rise to similar phenotypes, it suggests that D-mek is the only MEK activated by Drosophila RTKs. In addition, we demonstrate that different RTK pathways respond differently to alteration in D-Mek activity.</jats:p> A temperature-sensitive MEK mutation demonstrates the conservation of the signaling pathways activated by receptor tyrosine kinases. Genes & Development |
| spellingShingle | Hsu, J C, Perrimon, N, Genes & Development, A temperature-sensitive MEK mutation demonstrates the conservation of the signaling pathways activated by receptor tyrosine kinases., Developmental Biology, Genetics |
| title | A temperature-sensitive MEK mutation demonstrates the conservation of the signaling pathways activated by receptor tyrosine kinases. |
| title_full | A temperature-sensitive MEK mutation demonstrates the conservation of the signaling pathways activated by receptor tyrosine kinases. |
| title_fullStr | A temperature-sensitive MEK mutation demonstrates the conservation of the signaling pathways activated by receptor tyrosine kinases. |
| title_full_unstemmed | A temperature-sensitive MEK mutation demonstrates the conservation of the signaling pathways activated by receptor tyrosine kinases. |
| title_short | A temperature-sensitive MEK mutation demonstrates the conservation of the signaling pathways activated by receptor tyrosine kinases. |
| title_sort | a temperature-sensitive mek mutation demonstrates the conservation of the signaling pathways activated by receptor tyrosine kinases. |
| title_unstemmed | A temperature-sensitive MEK mutation demonstrates the conservation of the signaling pathways activated by receptor tyrosine kinases. |
| topic | Developmental Biology, Genetics |
| url | http://dx.doi.org/10.1101/gad.8.18.2176 |