Selective assembly of HIV-1 Vif-Cul5-ElonginB-ElonginC E3 ubiquitin ligase complex through a novel SOCS box and upstream cysteines

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Bibliographische Detailangaben
Zeitschriftentitel:	Genes & Development
Personen und Körperschaften:	Yu, Yunkai, Xiao, Zuoxiang, Ehrlich, Elana S., Yu, Xianghui, Yu, Xiao-Fang
In:	Genes & Development, 18, 2004, 23, S. 2867-2872
Format:	E-Article
Sprache:	Englisch
veröffentlicht:	Cold Spring Harbor Laboratory
Schlagwörter:	Developmental Biology Genetics

Details
Zusammenfassung:	<jats:p>APOBEC3G, which induces hypermutations in newly synthesized viral DNA, is suppressed by HIV-1 Vif, acting through Cul5-ElonginB-ElonginC E3 ubiquitin ligase. We have now characterized a novel SOCS box in HIV-1 Vif that mediates its interaction with ElonginC. In this SOCS box, alanine replaces the consensus cysteine in the previously identified SOCS box. This new motif was necessary but insufficient for interaction with Cul5-ElonginB-ElonginC, as two highly conserved Cys residues outside the SOCS box were required to interact with Cul5 but not ElonginC. Therefore, selective assembly with Cul5 versus Cul2 E3 may require protein interfaces besides the SOCS-box-ElonginC interaction.</jats:p>
Umfang:	2867-2872
ISSN:	0890-9369 1549-5477
DOI:	10.1101/gad.1250204