Smoothened transduces Hedgehog signal by physically interacting with Costal2/Fused complex through its C-terminal tail

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Bibliographische Detailangaben
Zeitschriftentitel:	Genes & Development
Personen und Körperschaften:	Jia, Jianhang, Tong, Chao, Jiang, Jin
In:	Genes & Development, 17, 2003, 21, S. 2709-2720
Format:	E-Article
Sprache:	Englisch
veröffentlicht:	Cold Spring Harbor Laboratory
Schlagwörter:	Developmental Biology Genetics

Details
Zusammenfassung:	<jats:p>The Hedgehog (Hh) family of secreted proteins controls many aspects of growth and patterning in animal development. The seven-transmembrane protein Smoothened (Smo) transduces the Hh signal in both vertebrates and invertebrates; however, the mechanism of its action remains unknown. We found that Smo lacking its C-terminal tail (C-tail) is inactive, whereas membrane-tethered Smo C-tail has constitutive albeit low levels of Hh signaling activity. Smo physically interacts with Costal2 (Cos2) and Fused (Fu) through its C-tail. Deletion of the Cos2/Fu-binding domain from Smo abolishes its signaling activity. Moreover, overexpressing Cos2 mutants that fail to bind Fu and Ci but retain Smo-binding activity blocks Hh signaling. Taken together, our results suggest that Smo transduces the Hh signal by physically interacting with the Cos2/Fu protein complex.</jats:p>
Umfang:	2709-2720
ISSN:	0890-9369 1549-5477
DOI:	10.1101/gad.1136603