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KNL-1 directs assembly of the microtubule-binding interface of the kinetochore in C. elegans
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| Zeitschriftentitel: | Genes & Development |
|---|---|
| Personen und Körperschaften: | , , , , , , |
| In: | Genes & Development, 17, 2003, 19, S. 2421-2435 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Cold Spring Harbor Laboratory
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| Schlagwörter: |
| author_facet |
Desai, Arshad Rybina, Sonja Müller-Reichert, Thomas Shevchenko, Andrej Shevchenko, Anna Hyman, Anthony Oegema, Karen Desai, Arshad Rybina, Sonja Müller-Reichert, Thomas Shevchenko, Andrej Shevchenko, Anna Hyman, Anthony Oegema, Karen |
|---|---|
| author |
Desai, Arshad Rybina, Sonja Müller-Reichert, Thomas Shevchenko, Andrej Shevchenko, Anna Hyman, Anthony Oegema, Karen |
| spellingShingle |
Desai, Arshad Rybina, Sonja Müller-Reichert, Thomas Shevchenko, Andrej Shevchenko, Anna Hyman, Anthony Oegema, Karen Genes & Development KNL-1 directs assembly of the microtubule-binding interface of the kinetochore in C. elegans Developmental Biology Genetics |
| author_sort |
desai, arshad |
| spelling |
Desai, Arshad Rybina, Sonja Müller-Reichert, Thomas Shevchenko, Andrej Shevchenko, Anna Hyman, Anthony Oegema, Karen 0890-9369 1549-5477 Cold Spring Harbor Laboratory Developmental Biology Genetics http://dx.doi.org/10.1101/gad.1126303 <jats:p>Segregation of the replicated genome during cell division requires kinetochores, mechanochemical organelles that assemble on mitotic chromosomes to connect them to spindle microtubules. CENP-A, a histone H3 variant, and CENP-C, a conserved structural protein, form the DNA-proximal foundation for kinetochore assembly. Using RNA interference-based genomics in <jats:italic>Caenorhabditis elegans</jats:italic>, we identified KNL-1, a novel kinetochore protein whose depletion, like that of CeCENP-A or CeCENP-C, leads to a “kinetochore-null” phenotype. KNL-1 is downstream of CeCENP-A and CeCENP-C in a linear assembly hierarchy. In embryonic extracts, KNL-1 exhibits substoichiometric interactions with CeCENP-C and forms a near-stoichiometric complex with CeNDC-80 and HIM-10, the <jats:italic>C. elegans</jats:italic> homologs of Ndc80p/HEC1p and Nuf2p—two widely conserved outer kinetochore components. However, CeNDC-80 and HIM-10 are not functionally equivalent to KNL-1 because their inhibition, although preventing formation of a mechanically stable kinetochore-microtubule interface and causing chromosome missegregation, does not result in a kinetochore-null phenotype. The greater functional importance of KNL-1 may be due to its requirement for targeting multiple components of the outer kinetochore, including CeNDC-80 and HIM-10. Thus, KNL-1 plays a central role in translating the initiation of kinetochore assembly by CeCENP-A and CeCENP-C into the formation of a functional microtubule-binding interface.</jats:p> KNL-1 directs assembly of the microtubule-binding interface of the kinetochore in <i>C. elegans</i> Genes & Development |
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10.1101/gad.1126303 |
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Online Free |
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Biologie |
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Cold Spring Harbor Laboratory, 2003 |
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Cold Spring Harbor Laboratory, 2003 |
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0890-9369 1549-5477 |
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0890-9369 1549-5477 |
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2003 |
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Genes & Development |
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| title |
KNL-1 directs assembly of the microtubule-binding interface of the kinetochore in C. elegans |
| title_unstemmed |
KNL-1 directs assembly of the microtubule-binding interface of the kinetochore in C. elegans |
| title_full |
KNL-1 directs assembly of the microtubule-binding interface of the kinetochore in C. elegans |
| title_fullStr |
KNL-1 directs assembly of the microtubule-binding interface of the kinetochore in C. elegans |
| title_full_unstemmed |
KNL-1 directs assembly of the microtubule-binding interface of the kinetochore in C. elegans |
| title_short |
KNL-1 directs assembly of the microtubule-binding interface of the kinetochore in C. elegans |
| title_sort |
knl-1 directs assembly of the microtubule-binding interface of the kinetochore in <i>c. elegans</i> |
| topic |
Developmental Biology Genetics |
| url |
http://dx.doi.org/10.1101/gad.1126303 |
| publishDate |
2003 |
| physical |
2421-2435 |
| description |
<jats:p>Segregation of the replicated genome during cell division requires kinetochores, mechanochemical organelles that assemble on mitotic chromosomes to connect them to spindle microtubules. CENP-A, a histone H3 variant, and CENP-C, a conserved structural protein, form the DNA-proximal foundation for kinetochore assembly. Using RNA interference-based genomics in <jats:italic>Caenorhabditis elegans</jats:italic>, we identified KNL-1, a novel kinetochore protein whose depletion, like that of CeCENP-A or CeCENP-C, leads to a “kinetochore-null” phenotype. KNL-1 is downstream of CeCENP-A and CeCENP-C in a linear assembly hierarchy. In embryonic extracts, KNL-1 exhibits substoichiometric interactions with CeCENP-C and forms a near-stoichiometric complex with CeNDC-80 and HIM-10, the <jats:italic>C. elegans</jats:italic> homologs of Ndc80p/HEC1p and Nuf2p—two widely conserved outer kinetochore components. However, CeNDC-80 and HIM-10 are not functionally equivalent to KNL-1 because their inhibition, although preventing formation of a mechanically stable kinetochore-microtubule interface and causing chromosome missegregation, does not result in a kinetochore-null phenotype. The greater functional importance of KNL-1 may be due to its requirement for targeting multiple components of the outer kinetochore, including CeNDC-80 and HIM-10. Thus, KNL-1 plays a central role in translating the initiation of kinetochore assembly by CeCENP-A and CeCENP-C into the formation of a functional microtubule-binding interface.</jats:p> |
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| author | Desai, Arshad, Rybina, Sonja, Müller-Reichert, Thomas, Shevchenko, Andrej, Shevchenko, Anna, Hyman, Anthony, Oegema, Karen |
| author_facet | Desai, Arshad, Rybina, Sonja, Müller-Reichert, Thomas, Shevchenko, Andrej, Shevchenko, Anna, Hyman, Anthony, Oegema, Karen, Desai, Arshad, Rybina, Sonja, Müller-Reichert, Thomas, Shevchenko, Andrej, Shevchenko, Anna, Hyman, Anthony, Oegema, Karen |
| author_sort | desai, arshad |
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| description | <jats:p>Segregation of the replicated genome during cell division requires kinetochores, mechanochemical organelles that assemble on mitotic chromosomes to connect them to spindle microtubules. CENP-A, a histone H3 variant, and CENP-C, a conserved structural protein, form the DNA-proximal foundation for kinetochore assembly. Using RNA interference-based genomics in <jats:italic>Caenorhabditis elegans</jats:italic>, we identified KNL-1, a novel kinetochore protein whose depletion, like that of CeCENP-A or CeCENP-C, leads to a “kinetochore-null” phenotype. KNL-1 is downstream of CeCENP-A and CeCENP-C in a linear assembly hierarchy. In embryonic extracts, KNL-1 exhibits substoichiometric interactions with CeCENP-C and forms a near-stoichiometric complex with CeNDC-80 and HIM-10, the <jats:italic>C. elegans</jats:italic> homologs of Ndc80p/HEC1p and Nuf2p—two widely conserved outer kinetochore components. However, CeNDC-80 and HIM-10 are not functionally equivalent to KNL-1 because their inhibition, although preventing formation of a mechanically stable kinetochore-microtubule interface and causing chromosome missegregation, does not result in a kinetochore-null phenotype. The greater functional importance of KNL-1 may be due to its requirement for targeting multiple components of the outer kinetochore, including CeNDC-80 and HIM-10. Thus, KNL-1 plays a central role in translating the initiation of kinetochore assembly by CeCENP-A and CeCENP-C into the formation of a functional microtubule-binding interface.</jats:p> |
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| imprint | Cold Spring Harbor Laboratory, 2003 |
| imprint_str_mv | Cold Spring Harbor Laboratory, 2003 |
| institution | DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-D161, DE-Zwi2 |
| issn | 0890-9369, 1549-5477 |
| issn_str_mv | 0890-9369, 1549-5477 |
| language | English |
| last_indexed | 2024-03-01T16:48:37.624Z |
| match_str | desai2003knl1directsassemblyofthemicrotubulebindinginterfaceofthekinetochoreincelegans |
| mega_collection | Cold Spring Harbor Laboratory (CrossRef) |
| physical | 2421-2435 |
| publishDate | 2003 |
| publishDateSort | 2003 |
| publisher | Cold Spring Harbor Laboratory |
| record_format | ai |
| recordtype | ai |
| series | Genes & Development |
| source_id | 49 |
| spelling | Desai, Arshad Rybina, Sonja Müller-Reichert, Thomas Shevchenko, Andrej Shevchenko, Anna Hyman, Anthony Oegema, Karen 0890-9369 1549-5477 Cold Spring Harbor Laboratory Developmental Biology Genetics http://dx.doi.org/10.1101/gad.1126303 <jats:p>Segregation of the replicated genome during cell division requires kinetochores, mechanochemical organelles that assemble on mitotic chromosomes to connect them to spindle microtubules. CENP-A, a histone H3 variant, and CENP-C, a conserved structural protein, form the DNA-proximal foundation for kinetochore assembly. Using RNA interference-based genomics in <jats:italic>Caenorhabditis elegans</jats:italic>, we identified KNL-1, a novel kinetochore protein whose depletion, like that of CeCENP-A or CeCENP-C, leads to a “kinetochore-null” phenotype. KNL-1 is downstream of CeCENP-A and CeCENP-C in a linear assembly hierarchy. In embryonic extracts, KNL-1 exhibits substoichiometric interactions with CeCENP-C and forms a near-stoichiometric complex with CeNDC-80 and HIM-10, the <jats:italic>C. elegans</jats:italic> homologs of Ndc80p/HEC1p and Nuf2p—two widely conserved outer kinetochore components. However, CeNDC-80 and HIM-10 are not functionally equivalent to KNL-1 because their inhibition, although preventing formation of a mechanically stable kinetochore-microtubule interface and causing chromosome missegregation, does not result in a kinetochore-null phenotype. The greater functional importance of KNL-1 may be due to its requirement for targeting multiple components of the outer kinetochore, including CeNDC-80 and HIM-10. Thus, KNL-1 plays a central role in translating the initiation of kinetochore assembly by CeCENP-A and CeCENP-C into the formation of a functional microtubule-binding interface.</jats:p> KNL-1 directs assembly of the microtubule-binding interface of the kinetochore in <i>C. elegans</i> Genes & Development |
| spellingShingle | Desai, Arshad, Rybina, Sonja, Müller-Reichert, Thomas, Shevchenko, Andrej, Shevchenko, Anna, Hyman, Anthony, Oegema, Karen, Genes & Development, KNL-1 directs assembly of the microtubule-binding interface of the kinetochore in C. elegans, Developmental Biology, Genetics |
| title | KNL-1 directs assembly of the microtubule-binding interface of the kinetochore in C. elegans |
| title_full | KNL-1 directs assembly of the microtubule-binding interface of the kinetochore in C. elegans |
| title_fullStr | KNL-1 directs assembly of the microtubule-binding interface of the kinetochore in C. elegans |
| title_full_unstemmed | KNL-1 directs assembly of the microtubule-binding interface of the kinetochore in C. elegans |
| title_short | KNL-1 directs assembly of the microtubule-binding interface of the kinetochore in C. elegans |
| title_sort | knl-1 directs assembly of the microtubule-binding interface of the kinetochore in <i>c. elegans</i> |
| title_unstemmed | KNL-1 directs assembly of the microtubule-binding interface of the kinetochore in C. elegans |
| topic | Developmental Biology, Genetics |
| url | http://dx.doi.org/10.1101/gad.1126303 |