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Spindle checkpoint regulates Cdc20p stability in Saccharomyces cerevisiae
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| Zeitschriftentitel: | Genes & Development |
|---|---|
| Personen und Körperschaften: | , |
| In: | Genes & Development, 18, 2004, 12, S. 1439-1451 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Cold Spring Harbor Laboratory
|
| Schlagwörter: |
| author_facet |
Pan, Jing Chen, Rey-Huei Pan, Jing Chen, Rey-Huei |
|---|---|
| author |
Pan, Jing Chen, Rey-Huei |
| spellingShingle |
Pan, Jing Chen, Rey-Huei Genes & Development Spindle checkpoint regulates Cdc20p stability in Saccharomyces cerevisiae Developmental Biology Genetics |
| author_sort |
pan, jing |
| spelling |
Pan, Jing Chen, Rey-Huei 0890-9369 1549-5477 Cold Spring Harbor Laboratory Developmental Biology Genetics http://dx.doi.org/10.1101/gad.1184204 <jats:p>The spindle checkpoint arrests cells at the metaphase-to-anaphase transition until all chromosomes have properly attached to the mitotic spindle. Checkpoint proteins Mad2p and Mad3p/BubR1p bind and inhibit Cdc20p, an activator for the anaphase-promoting complex (APC). We find that upon spindle checkpoint activation by microtubule inhibitors benomyl or nocodazole, wild-type <jats:italic>Saccharomyces cerevisiae</jats:italic> contains less Cdc20p than spindle checkpoint mutants do, whereas their <jats:italic>CDC20</jats:italic> mRNA levels are similar. The difference in Cdc20p levels correlates with their difference in the half-lives of Cdc20p, indicating that the spindle checkpoint destabilizes Cdc20p. This process requires the association between Cdc20p and Mad2p, and functional APC, but is independent of the known destruction boxes in Cdc20p and the other APC activator Cdh1p. Importantly, destabilization of Cdc20p is important for the spindle checkpoint, because a modest overexpression of Cdc20p causes benomyl sensitivity and premature Pds1p degradation in cells treated with nocodazole. Our study suggests that the spindle checkpoint reduces Cdc20p to below a certain threshold level to ensure a complete inhibition of Cdc20p before anaphase.</jats:p> Spindle checkpoint regulates Cdc20p stability in <i>Saccharomyces cerevisiae</i> Genes & Development |
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10.1101/gad.1184204 |
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Cold Spring Harbor Laboratory |
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Genes & Development |
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| title |
Spindle checkpoint regulates Cdc20p stability in Saccharomyces cerevisiae |
| title_unstemmed |
Spindle checkpoint regulates Cdc20p stability in Saccharomyces cerevisiae |
| title_full |
Spindle checkpoint regulates Cdc20p stability in Saccharomyces cerevisiae |
| title_fullStr |
Spindle checkpoint regulates Cdc20p stability in Saccharomyces cerevisiae |
| title_full_unstemmed |
Spindle checkpoint regulates Cdc20p stability in Saccharomyces cerevisiae |
| title_short |
Spindle checkpoint regulates Cdc20p stability in Saccharomyces cerevisiae |
| title_sort |
spindle checkpoint regulates cdc20p stability in <i>saccharomyces cerevisiae</i> |
| topic |
Developmental Biology Genetics |
| url |
http://dx.doi.org/10.1101/gad.1184204 |
| publishDate |
2004 |
| physical |
1439-1451 |
| description |
<jats:p>The spindle checkpoint arrests cells at the metaphase-to-anaphase transition until all chromosomes have properly attached to the mitotic spindle. Checkpoint proteins Mad2p and Mad3p/BubR1p bind and inhibit Cdc20p, an activator for the anaphase-promoting complex (APC). We find that upon spindle checkpoint activation by microtubule inhibitors benomyl or nocodazole, wild-type <jats:italic>Saccharomyces cerevisiae</jats:italic> contains less Cdc20p than spindle checkpoint mutants do, whereas their <jats:italic>CDC20</jats:italic> mRNA levels are similar. The difference in Cdc20p levels correlates with their difference in the half-lives of Cdc20p, indicating that the spindle checkpoint destabilizes Cdc20p. This process requires the association between Cdc20p and Mad2p, and functional APC, but is independent of the known destruction boxes in Cdc20p and the other APC activator Cdh1p. Importantly, destabilization of Cdc20p is important for the spindle checkpoint, because a modest overexpression of Cdc20p causes benomyl sensitivity and premature Pds1p degradation in cells treated with nocodazole. Our study suggests that the spindle checkpoint reduces Cdc20p to below a certain threshold level to ensure a complete inhibition of Cdc20p before anaphase.</jats:p> |
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| description | <jats:p>The spindle checkpoint arrests cells at the metaphase-to-anaphase transition until all chromosomes have properly attached to the mitotic spindle. Checkpoint proteins Mad2p and Mad3p/BubR1p bind and inhibit Cdc20p, an activator for the anaphase-promoting complex (APC). We find that upon spindle checkpoint activation by microtubule inhibitors benomyl or nocodazole, wild-type <jats:italic>Saccharomyces cerevisiae</jats:italic> contains less Cdc20p than spindle checkpoint mutants do, whereas their <jats:italic>CDC20</jats:italic> mRNA levels are similar. The difference in Cdc20p levels correlates with their difference in the half-lives of Cdc20p, indicating that the spindle checkpoint destabilizes Cdc20p. This process requires the association between Cdc20p and Mad2p, and functional APC, but is independent of the known destruction boxes in Cdc20p and the other APC activator Cdh1p. Importantly, destabilization of Cdc20p is important for the spindle checkpoint, because a modest overexpression of Cdc20p causes benomyl sensitivity and premature Pds1p degradation in cells treated with nocodazole. Our study suggests that the spindle checkpoint reduces Cdc20p to below a certain threshold level to ensure a complete inhibition of Cdc20p before anaphase.</jats:p> |
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| imprint | Cold Spring Harbor Laboratory, 2004 |
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| institution | DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229 |
| issn | 0890-9369, 1549-5477 |
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| spelling | Pan, Jing Chen, Rey-Huei 0890-9369 1549-5477 Cold Spring Harbor Laboratory Developmental Biology Genetics http://dx.doi.org/10.1101/gad.1184204 <jats:p>The spindle checkpoint arrests cells at the metaphase-to-anaphase transition until all chromosomes have properly attached to the mitotic spindle. Checkpoint proteins Mad2p and Mad3p/BubR1p bind and inhibit Cdc20p, an activator for the anaphase-promoting complex (APC). We find that upon spindle checkpoint activation by microtubule inhibitors benomyl or nocodazole, wild-type <jats:italic>Saccharomyces cerevisiae</jats:italic> contains less Cdc20p than spindle checkpoint mutants do, whereas their <jats:italic>CDC20</jats:italic> mRNA levels are similar. The difference in Cdc20p levels correlates with their difference in the half-lives of Cdc20p, indicating that the spindle checkpoint destabilizes Cdc20p. This process requires the association between Cdc20p and Mad2p, and functional APC, but is independent of the known destruction boxes in Cdc20p and the other APC activator Cdh1p. Importantly, destabilization of Cdc20p is important for the spindle checkpoint, because a modest overexpression of Cdc20p causes benomyl sensitivity and premature Pds1p degradation in cells treated with nocodazole. Our study suggests that the spindle checkpoint reduces Cdc20p to below a certain threshold level to ensure a complete inhibition of Cdc20p before anaphase.</jats:p> Spindle checkpoint regulates Cdc20p stability in <i>Saccharomyces cerevisiae</i> Genes & Development |
| spellingShingle | Pan, Jing, Chen, Rey-Huei, Genes & Development, Spindle checkpoint regulates Cdc20p stability in Saccharomyces cerevisiae, Developmental Biology, Genetics |
| title | Spindle checkpoint regulates Cdc20p stability in Saccharomyces cerevisiae |
| title_full | Spindle checkpoint regulates Cdc20p stability in Saccharomyces cerevisiae |
| title_fullStr | Spindle checkpoint regulates Cdc20p stability in Saccharomyces cerevisiae |
| title_full_unstemmed | Spindle checkpoint regulates Cdc20p stability in Saccharomyces cerevisiae |
| title_short | Spindle checkpoint regulates Cdc20p stability in Saccharomyces cerevisiae |
| title_sort | spindle checkpoint regulates cdc20p stability in <i>saccharomyces cerevisiae</i> |
| title_unstemmed | Spindle checkpoint regulates Cdc20p stability in Saccharomyces cerevisiae |
| topic | Developmental Biology, Genetics |
| url | http://dx.doi.org/10.1101/gad.1184204 |