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Sensitivity and Selectivity on Aptamer-Based Assay: The Determination of Tetracycline Residue in Bovine Milk
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| Zeitschriftentitel: | The Scientific World Journal |
|---|---|
| Personen und Körperschaften: | , |
| In: | The Scientific World Journal, 2012, 2012, S. 1-10 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Hindawi Limited
|
| Schlagwörter: |
| author_facet |
Jeong, Sohee Rhee Paeng, Insook Jeong, Sohee Rhee Paeng, Insook |
|---|---|
| author |
Jeong, Sohee Rhee Paeng, Insook |
| spellingShingle |
Jeong, Sohee Rhee Paeng, Insook The Scientific World Journal Sensitivity and Selectivity on Aptamer-Based Assay: The Determination of Tetracycline Residue in Bovine Milk General Environmental Science General Biochemistry, Genetics and Molecular Biology General Medicine |
| author_sort |
jeong, sohee |
| spelling |
Jeong, Sohee Rhee Paeng, Insook 1537-744X Hindawi Limited General Environmental Science General Biochemistry, Genetics and Molecular Biology General Medicine http://dx.doi.org/10.1100/2012/159456 <jats:p>A competitive enzyme-linked aptamer assay (ELAA) to detect tetracycline in milk was performed by using two different aptamers individually; one is 76 mer-DNA aptamer and the other is 57 mer-RNA aptamer. The best optimum condition was obtained without monovalent ion, Na<jats:sup>+</jats:sup>and also by adding no Mg<jats:sup>2+</jats:sup>ion in the assay buffer, along with RT incubation. The optimized ELAA showed a good sensitivity (LOD of 2.10 × 10<jats:sup>−8</jats:sup>M) with a wide dynamic range (3.16 × 10<jats:sup>−8</jats:sup>M ~ 3.16 × 10<jats:sup>−4</jats:sup>M). In addition, the average R.S.D. across all data points of the curve was less than 2.5% with good recoveries (~101.8%) from the milk media. Thus, this method provides a good tool to monitor tetracycline in milk from MRLs’ point of view. However, this ELAA method was not superior to the ELISA method in terms of specificity. This paper describes that it does not always give better sensitivity and specificity in assays even though aptamers have several advantages over antibodies and have been known to be good binders for binding assays.</jats:p> Sensitivity and Selectivity on Aptamer-Based Assay: The Determination of Tetracycline Residue in Bovine Milk The Scientific World Journal |
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| title |
Sensitivity and Selectivity on Aptamer-Based Assay: The Determination of Tetracycline Residue in Bovine Milk |
| title_unstemmed |
Sensitivity and Selectivity on Aptamer-Based Assay: The Determination of Tetracycline Residue in Bovine Milk |
| title_full |
Sensitivity and Selectivity on Aptamer-Based Assay: The Determination of Tetracycline Residue in Bovine Milk |
| title_fullStr |
Sensitivity and Selectivity on Aptamer-Based Assay: The Determination of Tetracycline Residue in Bovine Milk |
| title_full_unstemmed |
Sensitivity and Selectivity on Aptamer-Based Assay: The Determination of Tetracycline Residue in Bovine Milk |
| title_short |
Sensitivity and Selectivity on Aptamer-Based Assay: The Determination of Tetracycline Residue in Bovine Milk |
| title_sort |
sensitivity and selectivity on aptamer-based assay: the determination of tetracycline residue in bovine milk |
| topic |
General Environmental Science General Biochemistry, Genetics and Molecular Biology General Medicine |
| url |
http://dx.doi.org/10.1100/2012/159456 |
| publishDate |
2012 |
| physical |
1-10 |
| description |
<jats:p>A competitive enzyme-linked aptamer assay (ELAA) to detect tetracycline in milk was performed by using two different aptamers individually; one is 76 mer-DNA aptamer and the other is 57 mer-RNA aptamer. The best optimum condition was obtained without monovalent ion, Na<jats:sup>+</jats:sup>and also by adding no Mg<jats:sup>2+</jats:sup>ion in the assay buffer, along with RT incubation. The optimized ELAA showed a good sensitivity (LOD of 2.10 × 10<jats:sup>−8</jats:sup>M) with a wide dynamic range (3.16 × 10<jats:sup>−8</jats:sup>M ~ 3.16 × 10<jats:sup>−4</jats:sup>M). In addition, the average R.S.D. across all data points of the curve was less than 2.5% with good recoveries (~101.8%) from the milk media. Thus, this method provides a good tool to monitor tetracycline in milk from MRLs’ point of view. However, this ELAA method was not superior to the ELISA method in terms of specificity. This paper describes that it does not always give better sensitivity and specificity in assays even though aptamers have several advantages over antibodies and have been known to be good binders for binding assays.</jats:p> |
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| description | <jats:p>A competitive enzyme-linked aptamer assay (ELAA) to detect tetracycline in milk was performed by using two different aptamers individually; one is 76 mer-DNA aptamer and the other is 57 mer-RNA aptamer. The best optimum condition was obtained without monovalent ion, Na<jats:sup>+</jats:sup>and also by adding no Mg<jats:sup>2+</jats:sup>ion in the assay buffer, along with RT incubation. The optimized ELAA showed a good sensitivity (LOD of 2.10 × 10<jats:sup>−8</jats:sup>M) with a wide dynamic range (3.16 × 10<jats:sup>−8</jats:sup>M ~ 3.16 × 10<jats:sup>−4</jats:sup>M). In addition, the average R.S.D. across all data points of the curve was less than 2.5% with good recoveries (~101.8%) from the milk media. Thus, this method provides a good tool to monitor tetracycline in milk from MRLs’ point of view. However, this ELAA method was not superior to the ELISA method in terms of specificity. This paper describes that it does not always give better sensitivity and specificity in assays even though aptamers have several advantages over antibodies and have been known to be good binders for binding assays.</jats:p> |
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| spelling | Jeong, Sohee Rhee Paeng, Insook 1537-744X Hindawi Limited General Environmental Science General Biochemistry, Genetics and Molecular Biology General Medicine http://dx.doi.org/10.1100/2012/159456 <jats:p>A competitive enzyme-linked aptamer assay (ELAA) to detect tetracycline in milk was performed by using two different aptamers individually; one is 76 mer-DNA aptamer and the other is 57 mer-RNA aptamer. The best optimum condition was obtained without monovalent ion, Na<jats:sup>+</jats:sup>and also by adding no Mg<jats:sup>2+</jats:sup>ion in the assay buffer, along with RT incubation. The optimized ELAA showed a good sensitivity (LOD of 2.10 × 10<jats:sup>−8</jats:sup>M) with a wide dynamic range (3.16 × 10<jats:sup>−8</jats:sup>M ~ 3.16 × 10<jats:sup>−4</jats:sup>M). In addition, the average R.S.D. across all data points of the curve was less than 2.5% with good recoveries (~101.8%) from the milk media. Thus, this method provides a good tool to monitor tetracycline in milk from MRLs’ point of view. However, this ELAA method was not superior to the ELISA method in terms of specificity. This paper describes that it does not always give better sensitivity and specificity in assays even though aptamers have several advantages over antibodies and have been known to be good binders for binding assays.</jats:p> Sensitivity and Selectivity on Aptamer-Based Assay: The Determination of Tetracycline Residue in Bovine Milk The Scientific World Journal |
| spellingShingle | Jeong, Sohee, Rhee Paeng, Insook, The Scientific World Journal, Sensitivity and Selectivity on Aptamer-Based Assay: The Determination of Tetracycline Residue in Bovine Milk, General Environmental Science, General Biochemistry, Genetics and Molecular Biology, General Medicine |
| title | Sensitivity and Selectivity on Aptamer-Based Assay: The Determination of Tetracycline Residue in Bovine Milk |
| title_full | Sensitivity and Selectivity on Aptamer-Based Assay: The Determination of Tetracycline Residue in Bovine Milk |
| title_fullStr | Sensitivity and Selectivity on Aptamer-Based Assay: The Determination of Tetracycline Residue in Bovine Milk |
| title_full_unstemmed | Sensitivity and Selectivity on Aptamer-Based Assay: The Determination of Tetracycline Residue in Bovine Milk |
| title_short | Sensitivity and Selectivity on Aptamer-Based Assay: The Determination of Tetracycline Residue in Bovine Milk |
| title_sort | sensitivity and selectivity on aptamer-based assay: the determination of tetracycline residue in bovine milk |
| title_unstemmed | Sensitivity and Selectivity on Aptamer-Based Assay: The Determination of Tetracycline Residue in Bovine Milk |
| topic | General Environmental Science, General Biochemistry, Genetics and Molecular Biology, General Medicine |
| url | http://dx.doi.org/10.1100/2012/159456 |