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An alternatively spliced CXCL16 isoform expressed by dendritic cells is a secreted chemoattractant for CXCR6+ cells
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| Zeitschriftentitel: | Journal of Leukocyte Biology |
|---|---|
| Personen und Körperschaften: | , , , , , |
| In: | Journal of Leukocyte Biology, 87, 2010, 6, S. 1029-1039 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Oxford University Press (OUP)
|
| Schlagwörter: |
| author_facet |
van der Voort, Robbert Verweij, Viviènne de Witte, Theo M Lasonder, Edwin Adema, Gosse J Dolstra, Harry van der Voort, Robbert Verweij, Viviènne de Witte, Theo M Lasonder, Edwin Adema, Gosse J Dolstra, Harry |
|---|---|
| author |
van der Voort, Robbert Verweij, Viviènne de Witte, Theo M Lasonder, Edwin Adema, Gosse J Dolstra, Harry |
| spellingShingle |
van der Voort, Robbert Verweij, Viviènne de Witte, Theo M Lasonder, Edwin Adema, Gosse J Dolstra, Harry Journal of Leukocyte Biology An alternatively spliced CXCL16 isoform expressed by dendritic cells is a secreted chemoattractant for CXCR6+ cells Cell Biology Immunology Immunology and Allergy |
| author_sort |
van der voort, robbert |
| spelling |
van der Voort, Robbert Verweij, Viviènne de Witte, Theo M Lasonder, Edwin Adema, Gosse J Dolstra, Harry 1938-3673 0741-5400 Oxford University Press (OUP) Cell Biology Immunology Immunology and Allergy http://dx.doi.org/10.1189/jlb.0709482 <jats:title>Abstract</jats:title> <jats:p>A secreted isoform of the chemokine CXCL16 contributes to the interaction between dendritic cells and CXCR6+ lymphocytes.</jats:p> <jats:p>DC are professional APCs that initiate and regulate adaptive immune responses by interacting with naïve and memory T cells. Chemokines released by DC play an essential role in T cell recruitment and in the maintenance of antigen-specific T cell-DC conjugates. Here, we characterized the expression of the T cell-attracting chemokine CXCL16 by murine DC. We demonstrate that through alternative RNA splicing, DC not only express the previously characterized transmembrane CXCL16 isoform, which can be cleaved from the cell surface, but also a novel isoform lacking the transmembrane and cytoplasmic domains. Transfection of HEK293 cells shows that this novel isoform, termed CXCL16v, is not expressed on the cell membrane but is secreted as a protein of ∼10 kDa. Quantitative PCR demonstrates that CXCL16v is broadly expressed in lymphoid and nonlymphoid tissues resembling the tissue distribution of DC. Indeed, CXCL16v mRNA is expressed significantly by spleen DC and BM-DC. Moreover, we show that mature DC have increased CXCL16v mRNA levels and express transmembrane and soluble CXCL16 proteins. Finally, we show that CXCL16v specifically attracts cells expressing the chemokine receptor CXCR6. Our data demonstrate that mature DC express secreted, transmembrane, and cleaved CXCL16 isoforms to recruit and communicate efficiently with CXCR6+ lymphoid cells.</jats:p> An alternatively spliced CXCL16 isoform expressed by dendritic cells is a secreted chemoattractant for CXCR6+ cells Journal of Leukocyte Biology |
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| title |
An alternatively spliced CXCL16 isoform expressed by dendritic cells is a secreted chemoattractant for CXCR6+ cells |
| title_unstemmed |
An alternatively spliced CXCL16 isoform expressed by dendritic cells is a secreted chemoattractant for CXCR6+ cells |
| title_full |
An alternatively spliced CXCL16 isoform expressed by dendritic cells is a secreted chemoattractant for CXCR6+ cells |
| title_fullStr |
An alternatively spliced CXCL16 isoform expressed by dendritic cells is a secreted chemoattractant for CXCR6+ cells |
| title_full_unstemmed |
An alternatively spliced CXCL16 isoform expressed by dendritic cells is a secreted chemoattractant for CXCR6+ cells |
| title_short |
An alternatively spliced CXCL16 isoform expressed by dendritic cells is a secreted chemoattractant for CXCR6+ cells |
| title_sort |
an alternatively spliced cxcl16 isoform expressed by dendritic cells is a secreted chemoattractant for cxcr6+ cells |
| topic |
Cell Biology Immunology Immunology and Allergy |
| url |
http://dx.doi.org/10.1189/jlb.0709482 |
| publishDate |
2010 |
| physical |
1029-1039 |
| description |
<jats:title>Abstract</jats:title>
<jats:p>A secreted isoform of the chemokine CXCL16 contributes to the interaction between dendritic cells and CXCR6+ lymphocytes.</jats:p>
<jats:p>DC are professional APCs that initiate and regulate adaptive immune responses by interacting with naïve and memory T cells. Chemokines released by DC play an essential role in T cell recruitment and in the maintenance of antigen-specific T cell-DC conjugates. Here, we characterized the expression of the T cell-attracting chemokine CXCL16 by murine DC. We demonstrate that through alternative RNA splicing, DC not only express the previously characterized transmembrane CXCL16 isoform, which can be cleaved from the cell surface, but also a novel isoform lacking the transmembrane and cytoplasmic domains. Transfection of HEK293 cells shows that this novel isoform, termed CXCL16v, is not expressed on the cell membrane but is secreted as a protein of ∼10 kDa. Quantitative PCR demonstrates that CXCL16v is broadly expressed in lymphoid and nonlymphoid tissues resembling the tissue distribution of DC. Indeed, CXCL16v mRNA is expressed significantly by spleen DC and BM-DC. Moreover, we show that mature DC have increased CXCL16v mRNA levels and express transmembrane and soluble CXCL16 proteins. Finally, we show that CXCL16v specifically attracts cells expressing the chemokine receptor CXCR6. Our data demonstrate that mature DC express secreted, transmembrane, and cleaved CXCL16 isoforms to recruit and communicate efficiently with CXCR6+ lymphoid cells.</jats:p> |
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| author | van der Voort, Robbert, Verweij, Viviènne, de Witte, Theo M, Lasonder, Edwin, Adema, Gosse J, Dolstra, Harry |
| author_facet | van der Voort, Robbert, Verweij, Viviènne, de Witte, Theo M, Lasonder, Edwin, Adema, Gosse J, Dolstra, Harry, van der Voort, Robbert, Verweij, Viviènne, de Witte, Theo M, Lasonder, Edwin, Adema, Gosse J, Dolstra, Harry |
| author_sort | van der voort, robbert |
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| container_title | Journal of Leukocyte Biology |
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| description | <jats:title>Abstract</jats:title> <jats:p>A secreted isoform of the chemokine CXCL16 contributes to the interaction between dendritic cells and CXCR6+ lymphocytes.</jats:p> <jats:p>DC are professional APCs that initiate and regulate adaptive immune responses by interacting with naïve and memory T cells. Chemokines released by DC play an essential role in T cell recruitment and in the maintenance of antigen-specific T cell-DC conjugates. Here, we characterized the expression of the T cell-attracting chemokine CXCL16 by murine DC. We demonstrate that through alternative RNA splicing, DC not only express the previously characterized transmembrane CXCL16 isoform, which can be cleaved from the cell surface, but also a novel isoform lacking the transmembrane and cytoplasmic domains. Transfection of HEK293 cells shows that this novel isoform, termed CXCL16v, is not expressed on the cell membrane but is secreted as a protein of ∼10 kDa. Quantitative PCR demonstrates that CXCL16v is broadly expressed in lymphoid and nonlymphoid tissues resembling the tissue distribution of DC. Indeed, CXCL16v mRNA is expressed significantly by spleen DC and BM-DC. Moreover, we show that mature DC have increased CXCL16v mRNA levels and express transmembrane and soluble CXCL16 proteins. Finally, we show that CXCL16v specifically attracts cells expressing the chemokine receptor CXCR6. Our data demonstrate that mature DC express secreted, transmembrane, and cleaved CXCL16 isoforms to recruit and communicate efficiently with CXCR6+ lymphoid cells.</jats:p> |
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| imprint | Oxford University Press (OUP), 2010 |
| imprint_str_mv | Oxford University Press (OUP), 2010 |
| institution | DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229 |
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| spelling | van der Voort, Robbert Verweij, Viviènne de Witte, Theo M Lasonder, Edwin Adema, Gosse J Dolstra, Harry 1938-3673 0741-5400 Oxford University Press (OUP) Cell Biology Immunology Immunology and Allergy http://dx.doi.org/10.1189/jlb.0709482 <jats:title>Abstract</jats:title> <jats:p>A secreted isoform of the chemokine CXCL16 contributes to the interaction between dendritic cells and CXCR6+ lymphocytes.</jats:p> <jats:p>DC are professional APCs that initiate and regulate adaptive immune responses by interacting with naïve and memory T cells. Chemokines released by DC play an essential role in T cell recruitment and in the maintenance of antigen-specific T cell-DC conjugates. Here, we characterized the expression of the T cell-attracting chemokine CXCL16 by murine DC. We demonstrate that through alternative RNA splicing, DC not only express the previously characterized transmembrane CXCL16 isoform, which can be cleaved from the cell surface, but also a novel isoform lacking the transmembrane and cytoplasmic domains. Transfection of HEK293 cells shows that this novel isoform, termed CXCL16v, is not expressed on the cell membrane but is secreted as a protein of ∼10 kDa. Quantitative PCR demonstrates that CXCL16v is broadly expressed in lymphoid and nonlymphoid tissues resembling the tissue distribution of DC. Indeed, CXCL16v mRNA is expressed significantly by spleen DC and BM-DC. Moreover, we show that mature DC have increased CXCL16v mRNA levels and express transmembrane and soluble CXCL16 proteins. Finally, we show that CXCL16v specifically attracts cells expressing the chemokine receptor CXCR6. Our data demonstrate that mature DC express secreted, transmembrane, and cleaved CXCL16 isoforms to recruit and communicate efficiently with CXCR6+ lymphoid cells.</jats:p> An alternatively spliced CXCL16 isoform expressed by dendritic cells is a secreted chemoattractant for CXCR6+ cells Journal of Leukocyte Biology |
| spellingShingle | van der Voort, Robbert, Verweij, Viviènne, de Witte, Theo M, Lasonder, Edwin, Adema, Gosse J, Dolstra, Harry, Journal of Leukocyte Biology, An alternatively spliced CXCL16 isoform expressed by dendritic cells is a secreted chemoattractant for CXCR6+ cells, Cell Biology, Immunology, Immunology and Allergy |
| title | An alternatively spliced CXCL16 isoform expressed by dendritic cells is a secreted chemoattractant for CXCR6+ cells |
| title_full | An alternatively spliced CXCL16 isoform expressed by dendritic cells is a secreted chemoattractant for CXCR6+ cells |
| title_fullStr | An alternatively spliced CXCL16 isoform expressed by dendritic cells is a secreted chemoattractant for CXCR6+ cells |
| title_full_unstemmed | An alternatively spliced CXCL16 isoform expressed by dendritic cells is a secreted chemoattractant for CXCR6+ cells |
| title_short | An alternatively spliced CXCL16 isoform expressed by dendritic cells is a secreted chemoattractant for CXCR6+ cells |
| title_sort | an alternatively spliced cxcl16 isoform expressed by dendritic cells is a secreted chemoattractant for cxcr6+ cells |
| title_unstemmed | An alternatively spliced CXCL16 isoform expressed by dendritic cells is a secreted chemoattractant for CXCR6+ cells |
| topic | Cell Biology, Immunology, Immunology and Allergy |
| url | http://dx.doi.org/10.1189/jlb.0709482 |