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Differentiation of two types of mobilized peripheral blood stem cells by microRNA and cDNA expression analysis
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Zeitschriftentitel: | Journal of Translational Medicine |
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Personen und Körperschaften: | , , , , , , , |
In: | Journal of Translational Medicine, 6, 2008, 1 |
Format: | E-Article |
Sprache: | Englisch |
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Springer Science and Business Media LLC
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author_facet |
Jin, Ping Wang, Ena Ren, Jiaqiang Childs, Richard Shin, Jeong Won Khuu, Hanh Marincola, Francesco M Stroncek, David F Jin, Ping Wang, Ena Ren, Jiaqiang Childs, Richard Shin, Jeong Won Khuu, Hanh Marincola, Francesco M Stroncek, David F |
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author |
Jin, Ping Wang, Ena Ren, Jiaqiang Childs, Richard Shin, Jeong Won Khuu, Hanh Marincola, Francesco M Stroncek, David F |
spellingShingle |
Jin, Ping Wang, Ena Ren, Jiaqiang Childs, Richard Shin, Jeong Won Khuu, Hanh Marincola, Francesco M Stroncek, David F Journal of Translational Medicine Differentiation of two types of mobilized peripheral blood stem cells by microRNA and cDNA expression analysis General Biochemistry, Genetics and Molecular Biology General Medicine |
author_sort |
jin, ping |
spelling |
Jin, Ping Wang, Ena Ren, Jiaqiang Childs, Richard Shin, Jeong Won Khuu, Hanh Marincola, Francesco M Stroncek, David F 1479-5876 Springer Science and Business Media LLC General Biochemistry, Genetics and Molecular Biology General Medicine http://dx.doi.org/10.1186/1479-5876-6-39 <jats:title>Abstract</jats:title> <jats:sec> <jats:title>Background</jats:title> <jats:p>Mobilized-peripheral blood hematopoietic stem cells (HSCs) have been used for transplantation, immunotherapy, and cardiovascular regenerative medicine. Agents used for HSC mobilization include G-CSF and the CXCR4 inhibitor AMD3100 (plerixafor). The HSCs cells mobilized by each agent may contain different subtypes and have different functions. To characterize mobilized HSCs used for clinical applications, microRNA (miRNA) profiling and gene expression profiling were used to compare AMD3100-mobilized CD133+ cells from 4 subjects, AMD3100 plus G-CSF-mobilized CD133+ cells from 4 subjects and G-CSF-mobilized CD34+ cells from 5 subjects. The HSCs were compared to peripheral blood leukocytes (PBLs) from 7 subjects.</jats:p> </jats:sec> <jats:sec> <jats:title>Results</jats:title> <jats:p>Hierarchical clustering of miRNAs separated HSCs from PBLs. miRNAs up-regulated in all HSCs included hematopoiesis-associated miRNA; miR-126, miR-10a, miR-221 and miR-17-92 cluster. miRNAs up-regulated in PBLs included miR-142-3p, -218, -21, and -379. Hierarchical clustering analysis of miRNA expression separated the AMD3100-mobilized CD133+ cells from G-CSF-mobilized CD34+ cells. Gene expression analysis of the HSCs naturally segregated samples according to mobilization and isolation protocol and cell differentiation status.</jats:p> </jats:sec> <jats:sec> <jats:title>Conclusion</jats:title> <jats:p>HSCs and PBLs have unique miRNA and gene expression profiles. miRNA and gene expression microarrays maybe useful for assessing differences in HSCs.</jats:p> </jats:sec> Differentiation of two types of mobilized peripheral blood stem cells by microRNA and cDNA expression analysis Journal of Translational Medicine |
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10.1186/1479-5876-6-39 |
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Online Free |
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Springer Science and Business Media LLC, 2008 |
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2008 |
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Springer Science and Business Media LLC |
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ai |
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Journal of Translational Medicine |
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49 |
title |
Differentiation of two types of mobilized peripheral blood stem cells by microRNA and cDNA expression analysis |
title_unstemmed |
Differentiation of two types of mobilized peripheral blood stem cells by microRNA and cDNA expression analysis |
title_full |
Differentiation of two types of mobilized peripheral blood stem cells by microRNA and cDNA expression analysis |
title_fullStr |
Differentiation of two types of mobilized peripheral blood stem cells by microRNA and cDNA expression analysis |
title_full_unstemmed |
Differentiation of two types of mobilized peripheral blood stem cells by microRNA and cDNA expression analysis |
title_short |
Differentiation of two types of mobilized peripheral blood stem cells by microRNA and cDNA expression analysis |
title_sort |
differentiation of two types of mobilized peripheral blood stem cells by microrna and cdna expression analysis |
topic |
General Biochemistry, Genetics and Molecular Biology General Medicine |
url |
http://dx.doi.org/10.1186/1479-5876-6-39 |
publishDate |
2008 |
physical |
|
description |
<jats:title>Abstract</jats:title>
<jats:sec>
<jats:title>Background</jats:title>
<jats:p>Mobilized-peripheral blood hematopoietic stem cells (HSCs) have been used for transplantation, immunotherapy, and cardiovascular regenerative medicine. Agents used for HSC mobilization include G-CSF and the CXCR4 inhibitor AMD3100 (plerixafor). The HSCs cells mobilized by each agent may contain different subtypes and have different functions. To characterize mobilized HSCs used for clinical applications, microRNA (miRNA) profiling and gene expression profiling were used to compare AMD3100-mobilized CD133+ cells from 4 subjects, AMD3100 plus G-CSF-mobilized CD133+ cells from 4 subjects and G-CSF-mobilized CD34+ cells from 5 subjects. The HSCs were compared to peripheral blood leukocytes (PBLs) from 7 subjects.</jats:p>
</jats:sec>
<jats:sec>
<jats:title>Results</jats:title>
<jats:p>Hierarchical clustering of miRNAs separated HSCs from PBLs. miRNAs up-regulated in all HSCs included hematopoiesis-associated miRNA; miR-126, miR-10a, miR-221 and miR-17-92 cluster. miRNAs up-regulated in PBLs included miR-142-3p, -218, -21, and -379. Hierarchical clustering analysis of miRNA expression separated the AMD3100-mobilized CD133+ cells from G-CSF-mobilized CD34+ cells. Gene expression analysis of the HSCs naturally segregated samples according to mobilization and isolation protocol and cell differentiation status.</jats:p>
</jats:sec>
<jats:sec>
<jats:title>Conclusion</jats:title>
<jats:p>HSCs and PBLs have unique miRNA and gene expression profiles. miRNA and gene expression microarrays maybe useful for assessing differences in HSCs.</jats:p>
</jats:sec> |
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author | Jin, Ping, Wang, Ena, Ren, Jiaqiang, Childs, Richard, Shin, Jeong Won, Khuu, Hanh, Marincola, Francesco M, Stroncek, David F |
author_facet | Jin, Ping, Wang, Ena, Ren, Jiaqiang, Childs, Richard, Shin, Jeong Won, Khuu, Hanh, Marincola, Francesco M, Stroncek, David F, Jin, Ping, Wang, Ena, Ren, Jiaqiang, Childs, Richard, Shin, Jeong Won, Khuu, Hanh, Marincola, Francesco M, Stroncek, David F |
author_sort | jin, ping |
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container_title | Journal of Translational Medicine |
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description | <jats:title>Abstract</jats:title> <jats:sec> <jats:title>Background</jats:title> <jats:p>Mobilized-peripheral blood hematopoietic stem cells (HSCs) have been used for transplantation, immunotherapy, and cardiovascular regenerative medicine. Agents used for HSC mobilization include G-CSF and the CXCR4 inhibitor AMD3100 (plerixafor). The HSCs cells mobilized by each agent may contain different subtypes and have different functions. To characterize mobilized HSCs used for clinical applications, microRNA (miRNA) profiling and gene expression profiling were used to compare AMD3100-mobilized CD133+ cells from 4 subjects, AMD3100 plus G-CSF-mobilized CD133+ cells from 4 subjects and G-CSF-mobilized CD34+ cells from 5 subjects. The HSCs were compared to peripheral blood leukocytes (PBLs) from 7 subjects.</jats:p> </jats:sec> <jats:sec> <jats:title>Results</jats:title> <jats:p>Hierarchical clustering of miRNAs separated HSCs from PBLs. miRNAs up-regulated in all HSCs included hematopoiesis-associated miRNA; miR-126, miR-10a, miR-221 and miR-17-92 cluster. miRNAs up-regulated in PBLs included miR-142-3p, -218, -21, and -379. Hierarchical clustering analysis of miRNA expression separated the AMD3100-mobilized CD133+ cells from G-CSF-mobilized CD34+ cells. Gene expression analysis of the HSCs naturally segregated samples according to mobilization and isolation protocol and cell differentiation status.</jats:p> </jats:sec> <jats:sec> <jats:title>Conclusion</jats:title> <jats:p>HSCs and PBLs have unique miRNA and gene expression profiles. miRNA and gene expression microarrays maybe useful for assessing differences in HSCs.</jats:p> </jats:sec> |
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spelling | Jin, Ping Wang, Ena Ren, Jiaqiang Childs, Richard Shin, Jeong Won Khuu, Hanh Marincola, Francesco M Stroncek, David F 1479-5876 Springer Science and Business Media LLC General Biochemistry, Genetics and Molecular Biology General Medicine http://dx.doi.org/10.1186/1479-5876-6-39 <jats:title>Abstract</jats:title> <jats:sec> <jats:title>Background</jats:title> <jats:p>Mobilized-peripheral blood hematopoietic stem cells (HSCs) have been used for transplantation, immunotherapy, and cardiovascular regenerative medicine. Agents used for HSC mobilization include G-CSF and the CXCR4 inhibitor AMD3100 (plerixafor). The HSCs cells mobilized by each agent may contain different subtypes and have different functions. To characterize mobilized HSCs used for clinical applications, microRNA (miRNA) profiling and gene expression profiling were used to compare AMD3100-mobilized CD133+ cells from 4 subjects, AMD3100 plus G-CSF-mobilized CD133+ cells from 4 subjects and G-CSF-mobilized CD34+ cells from 5 subjects. The HSCs were compared to peripheral blood leukocytes (PBLs) from 7 subjects.</jats:p> </jats:sec> <jats:sec> <jats:title>Results</jats:title> <jats:p>Hierarchical clustering of miRNAs separated HSCs from PBLs. miRNAs up-regulated in all HSCs included hematopoiesis-associated miRNA; miR-126, miR-10a, miR-221 and miR-17-92 cluster. miRNAs up-regulated in PBLs included miR-142-3p, -218, -21, and -379. Hierarchical clustering analysis of miRNA expression separated the AMD3100-mobilized CD133+ cells from G-CSF-mobilized CD34+ cells. Gene expression analysis of the HSCs naturally segregated samples according to mobilization and isolation protocol and cell differentiation status.</jats:p> </jats:sec> <jats:sec> <jats:title>Conclusion</jats:title> <jats:p>HSCs and PBLs have unique miRNA and gene expression profiles. miRNA and gene expression microarrays maybe useful for assessing differences in HSCs.</jats:p> </jats:sec> Differentiation of two types of mobilized peripheral blood stem cells by microRNA and cDNA expression analysis Journal of Translational Medicine |
spellingShingle | Jin, Ping, Wang, Ena, Ren, Jiaqiang, Childs, Richard, Shin, Jeong Won, Khuu, Hanh, Marincola, Francesco M, Stroncek, David F, Journal of Translational Medicine, Differentiation of two types of mobilized peripheral blood stem cells by microRNA and cDNA expression analysis, General Biochemistry, Genetics and Molecular Biology, General Medicine |
title | Differentiation of two types of mobilized peripheral blood stem cells by microRNA and cDNA expression analysis |
title_full | Differentiation of two types of mobilized peripheral blood stem cells by microRNA and cDNA expression analysis |
title_fullStr | Differentiation of two types of mobilized peripheral blood stem cells by microRNA and cDNA expression analysis |
title_full_unstemmed | Differentiation of two types of mobilized peripheral blood stem cells by microRNA and cDNA expression analysis |
title_short | Differentiation of two types of mobilized peripheral blood stem cells by microRNA and cDNA expression analysis |
title_sort | differentiation of two types of mobilized peripheral blood stem cells by microrna and cdna expression analysis |
title_unstemmed | Differentiation of two types of mobilized peripheral blood stem cells by microRNA and cDNA expression analysis |
topic | General Biochemistry, Genetics and Molecular Biology, General Medicine |
url | http://dx.doi.org/10.1186/1479-5876-6-39 |