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A multidisciplinary approach to study a couple of monozygotic twins discordant for the chronic fatigue syndrome: a focus on potential salivary biomarkers
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| Zeitschriftentitel: | Journal of Translational Medicine |
|---|---|
| Personen und Körperschaften: | , , , , , , , , , , |
| In: | Journal of Translational Medicine, 11, 2013, 1 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Springer Science and Business Media LLC
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| Schlagwörter: |
| author_facet |
Ciregia, Federica Giusti, Laura Da Valle, Ylenia Donadio, Elena Consensi, Arianna Giacomelli, Camillo Sernissi, Francesca Scarpellini, Pietro Maggi, Fabrizio Lucacchini, Antonio Bazzichi, Laura Ciregia, Federica Giusti, Laura Da Valle, Ylenia Donadio, Elena Consensi, Arianna Giacomelli, Camillo Sernissi, Francesca Scarpellini, Pietro Maggi, Fabrizio Lucacchini, Antonio Bazzichi, Laura |
|---|---|
| author |
Ciregia, Federica Giusti, Laura Da Valle, Ylenia Donadio, Elena Consensi, Arianna Giacomelli, Camillo Sernissi, Francesca Scarpellini, Pietro Maggi, Fabrizio Lucacchini, Antonio Bazzichi, Laura |
| spellingShingle |
Ciregia, Federica Giusti, Laura Da Valle, Ylenia Donadio, Elena Consensi, Arianna Giacomelli, Camillo Sernissi, Francesca Scarpellini, Pietro Maggi, Fabrizio Lucacchini, Antonio Bazzichi, Laura Journal of Translational Medicine A multidisciplinary approach to study a couple of monozygotic twins discordant for the chronic fatigue syndrome: a focus on potential salivary biomarkers General Biochemistry, Genetics and Molecular Biology General Medicine |
| author_sort |
ciregia, federica |
| spelling |
Ciregia, Federica Giusti, Laura Da Valle, Ylenia Donadio, Elena Consensi, Arianna Giacomelli, Camillo Sernissi, Francesca Scarpellini, Pietro Maggi, Fabrizio Lucacchini, Antonio Bazzichi, Laura 1479-5876 Springer Science and Business Media LLC General Biochemistry, Genetics and Molecular Biology General Medicine http://dx.doi.org/10.1186/1479-5876-11-243 <jats:title>Abstract</jats:title> <jats:sec> <jats:title>Background</jats:title> <jats:p>Chronic Fatigue Syndrome (CFS) is a severe, systemic illness characterized by persistent, debilitating and medically unexplained fatigue. The etiology and pathophysiology of CFS remains obscure, and diagnosis is formulated through the patient’s history and exclusion of other medical causes. Thereby, the availability of biomarkers for CFS could be useful for clinical research. In the present study, we used a proteomic approach to evaluate the global changes in the salivary profile in a couple of monozygotic twins who were discordant for CFS. The aim was to evaluate differences of salivary protein expression in the CFS patient in respect to his healthy twin.</jats:p> </jats:sec> <jats:sec> <jats:title>Methods</jats:title> <jats:p>Saliva samples were submitted to two-dimensional electrophoresis (2DE). The gels were stained with Sypro, and a comparison between CFS subject and the healthy one was performed by the software Progenesis Same Spot including the Analysis of variance (ANOVA test). The proteins spot found with a ≥2-fold spot quantity change and p<0.05 were identified by Nano-liquid chromatography electrospray ionization tandem mass spectrometry. To validate the expression changes found with 2DE of 5 proteins (14-3-3 protein zeta/delta, cyclophilin A, Cystatin-C, Protein S100-A7, and zinc-alpha-2-glycoprotein), we used the western blot analysis. Moreover, proteins differentially expressed were functionally analyzed using the Ingenuity Pathways Analysis software with the aim to determine the predominant canonical pathways and the interaction network involved.</jats:p> </jats:sec> <jats:sec> <jats:title>Results</jats:title> <jats:p>The analysis of the protein profiles allowed us to find 13 proteins with a different expression in CFS in respect to control. Nine spots were up-regulated in CFS and 4 down-regulated. These proteins belong to different functional classes, such as inflammatory response, immune system and metabolism. In particular, as shown by the pathway analysis, the network built with our proteins highlights the involvement of inflammatory response in CFS pathogenesis.</jats:p> </jats:sec> <jats:sec> <jats:title>Conclusions</jats:title> <jats:p>This study shows the presence of differentially expressed proteins in the saliva of the couple of monozygotic twins discordant for CFS, probably related to the disease. Consequently, we believe the proteomic approach could be useful both to define a panel of potential diagnostic biomarkers and to shed new light on the comprehension of the pathogenetic pathways of CFS.</jats:p> </jats:sec> A multidisciplinary approach to study a couple of monozygotic twins discordant for the chronic fatigue syndrome: a focus on potential salivary biomarkers Journal of Translational Medicine |
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Springer Science and Business Media LLC, 2013 |
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Springer Science and Business Media LLC, 2013 |
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ciregia2013amultidisciplinaryapproachtostudyacoupleofmonozygotictwinsdiscordantforthechronicfatiguesyndromeafocusonpotentialsalivarybiomarkers |
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2013 |
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Journal of Translational Medicine |
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| title |
A multidisciplinary approach to study a couple of monozygotic twins discordant for the chronic fatigue syndrome: a focus on potential salivary biomarkers |
| title_unstemmed |
A multidisciplinary approach to study a couple of monozygotic twins discordant for the chronic fatigue syndrome: a focus on potential salivary biomarkers |
| title_full |
A multidisciplinary approach to study a couple of monozygotic twins discordant for the chronic fatigue syndrome: a focus on potential salivary biomarkers |
| title_fullStr |
A multidisciplinary approach to study a couple of monozygotic twins discordant for the chronic fatigue syndrome: a focus on potential salivary biomarkers |
| title_full_unstemmed |
A multidisciplinary approach to study a couple of monozygotic twins discordant for the chronic fatigue syndrome: a focus on potential salivary biomarkers |
| title_short |
A multidisciplinary approach to study a couple of monozygotic twins discordant for the chronic fatigue syndrome: a focus on potential salivary biomarkers |
| title_sort |
a multidisciplinary approach to study a couple of monozygotic twins discordant for the chronic fatigue syndrome: a focus on potential salivary biomarkers |
| topic |
General Biochemistry, Genetics and Molecular Biology General Medicine |
| url |
http://dx.doi.org/10.1186/1479-5876-11-243 |
| publishDate |
2013 |
| physical |
|
| description |
<jats:title>Abstract</jats:title>
<jats:sec>
<jats:title>Background</jats:title>
<jats:p>Chronic Fatigue Syndrome (CFS) is a severe, systemic illness characterized by persistent, debilitating and medically unexplained fatigue. The etiology and pathophysiology of CFS remains obscure, and diagnosis is formulated through the patient’s history and exclusion of other medical causes. Thereby, the availability of biomarkers for CFS could be useful for clinical research. In the present study, we used a proteomic approach to evaluate the global changes in the salivary profile in a couple of monozygotic twins who were discordant for CFS. The aim was to evaluate differences of salivary protein expression in the CFS patient in respect to his healthy twin.</jats:p>
</jats:sec>
<jats:sec>
<jats:title>Methods</jats:title>
<jats:p>Saliva samples were submitted to two-dimensional electrophoresis (2DE). The gels were stained with Sypro, and a comparison between CFS subject and the healthy one was performed by the software Progenesis Same Spot including the Analysis of variance (ANOVA test). The proteins spot found with a ≥2-fold spot quantity change and p<0.05 were identified by Nano-liquid chromatography electrospray ionization tandem mass spectrometry. To validate the expression changes found with 2DE of 5 proteins (14-3-3 protein zeta/delta, cyclophilin A, Cystatin-C, Protein S100-A7, and zinc-alpha-2-glycoprotein), we used the western blot analysis. Moreover, proteins differentially expressed were functionally analyzed using the Ingenuity Pathways Analysis software with the aim to determine the predominant canonical pathways and the interaction network involved.</jats:p>
</jats:sec>
<jats:sec>
<jats:title>Results</jats:title>
<jats:p>The analysis of the protein profiles allowed us to find 13 proteins with a different expression in CFS in respect to control. Nine spots were up-regulated in CFS and 4 down-regulated. These proteins belong to different functional classes, such as inflammatory response, immune system and metabolism. In particular, as shown by the pathway analysis, the network built with our proteins highlights the involvement of inflammatory response in CFS pathogenesis.</jats:p>
</jats:sec>
<jats:sec>
<jats:title>Conclusions</jats:title>
<jats:p>This study shows the presence of differentially expressed proteins in the saliva of the couple of monozygotic twins discordant for CFS, probably related to the disease. Consequently, we believe the proteomic approach could be useful both to define a panel of potential diagnostic biomarkers and to shed new light on the comprehension of the pathogenetic pathways of CFS.</jats:p>
</jats:sec> |
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| author | Ciregia, Federica, Giusti, Laura, Da Valle, Ylenia, Donadio, Elena, Consensi, Arianna, Giacomelli, Camillo, Sernissi, Francesca, Scarpellini, Pietro, Maggi, Fabrizio, Lucacchini, Antonio, Bazzichi, Laura |
| author_facet | Ciregia, Federica, Giusti, Laura, Da Valle, Ylenia, Donadio, Elena, Consensi, Arianna, Giacomelli, Camillo, Sernissi, Francesca, Scarpellini, Pietro, Maggi, Fabrizio, Lucacchini, Antonio, Bazzichi, Laura, Ciregia, Federica, Giusti, Laura, Da Valle, Ylenia, Donadio, Elena, Consensi, Arianna, Giacomelli, Camillo, Sernissi, Francesca, Scarpellini, Pietro, Maggi, Fabrizio, Lucacchini, Antonio, Bazzichi, Laura |
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| description | <jats:title>Abstract</jats:title> <jats:sec> <jats:title>Background</jats:title> <jats:p>Chronic Fatigue Syndrome (CFS) is a severe, systemic illness characterized by persistent, debilitating and medically unexplained fatigue. The etiology and pathophysiology of CFS remains obscure, and diagnosis is formulated through the patient’s history and exclusion of other medical causes. Thereby, the availability of biomarkers for CFS could be useful for clinical research. In the present study, we used a proteomic approach to evaluate the global changes in the salivary profile in a couple of monozygotic twins who were discordant for CFS. The aim was to evaluate differences of salivary protein expression in the CFS patient in respect to his healthy twin.</jats:p> </jats:sec> <jats:sec> <jats:title>Methods</jats:title> <jats:p>Saliva samples were submitted to two-dimensional electrophoresis (2DE). The gels were stained with Sypro, and a comparison between CFS subject and the healthy one was performed by the software Progenesis Same Spot including the Analysis of variance (ANOVA test). The proteins spot found with a ≥2-fold spot quantity change and p<0.05 were identified by Nano-liquid chromatography electrospray ionization tandem mass spectrometry. To validate the expression changes found with 2DE of 5 proteins (14-3-3 protein zeta/delta, cyclophilin A, Cystatin-C, Protein S100-A7, and zinc-alpha-2-glycoprotein), we used the western blot analysis. Moreover, proteins differentially expressed were functionally analyzed using the Ingenuity Pathways Analysis software with the aim to determine the predominant canonical pathways and the interaction network involved.</jats:p> </jats:sec> <jats:sec> <jats:title>Results</jats:title> <jats:p>The analysis of the protein profiles allowed us to find 13 proteins with a different expression in CFS in respect to control. Nine spots were up-regulated in CFS and 4 down-regulated. These proteins belong to different functional classes, such as inflammatory response, immune system and metabolism. In particular, as shown by the pathway analysis, the network built with our proteins highlights the involvement of inflammatory response in CFS pathogenesis.</jats:p> </jats:sec> <jats:sec> <jats:title>Conclusions</jats:title> <jats:p>This study shows the presence of differentially expressed proteins in the saliva of the couple of monozygotic twins discordant for CFS, probably related to the disease. Consequently, we believe the proteomic approach could be useful both to define a panel of potential diagnostic biomarkers and to shed new light on the comprehension of the pathogenetic pathways of CFS.</jats:p> </jats:sec> |
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| spelling | Ciregia, Federica Giusti, Laura Da Valle, Ylenia Donadio, Elena Consensi, Arianna Giacomelli, Camillo Sernissi, Francesca Scarpellini, Pietro Maggi, Fabrizio Lucacchini, Antonio Bazzichi, Laura 1479-5876 Springer Science and Business Media LLC General Biochemistry, Genetics and Molecular Biology General Medicine http://dx.doi.org/10.1186/1479-5876-11-243 <jats:title>Abstract</jats:title> <jats:sec> <jats:title>Background</jats:title> <jats:p>Chronic Fatigue Syndrome (CFS) is a severe, systemic illness characterized by persistent, debilitating and medically unexplained fatigue. The etiology and pathophysiology of CFS remains obscure, and diagnosis is formulated through the patient’s history and exclusion of other medical causes. Thereby, the availability of biomarkers for CFS could be useful for clinical research. In the present study, we used a proteomic approach to evaluate the global changes in the salivary profile in a couple of monozygotic twins who were discordant for CFS. The aim was to evaluate differences of salivary protein expression in the CFS patient in respect to his healthy twin.</jats:p> </jats:sec> <jats:sec> <jats:title>Methods</jats:title> <jats:p>Saliva samples were submitted to two-dimensional electrophoresis (2DE). The gels were stained with Sypro, and a comparison between CFS subject and the healthy one was performed by the software Progenesis Same Spot including the Analysis of variance (ANOVA test). The proteins spot found with a ≥2-fold spot quantity change and p<0.05 were identified by Nano-liquid chromatography electrospray ionization tandem mass spectrometry. To validate the expression changes found with 2DE of 5 proteins (14-3-3 protein zeta/delta, cyclophilin A, Cystatin-C, Protein S100-A7, and zinc-alpha-2-glycoprotein), we used the western blot analysis. Moreover, proteins differentially expressed were functionally analyzed using the Ingenuity Pathways Analysis software with the aim to determine the predominant canonical pathways and the interaction network involved.</jats:p> </jats:sec> <jats:sec> <jats:title>Results</jats:title> <jats:p>The analysis of the protein profiles allowed us to find 13 proteins with a different expression in CFS in respect to control. Nine spots were up-regulated in CFS and 4 down-regulated. These proteins belong to different functional classes, such as inflammatory response, immune system and metabolism. In particular, as shown by the pathway analysis, the network built with our proteins highlights the involvement of inflammatory response in CFS pathogenesis.</jats:p> </jats:sec> <jats:sec> <jats:title>Conclusions</jats:title> <jats:p>This study shows the presence of differentially expressed proteins in the saliva of the couple of monozygotic twins discordant for CFS, probably related to the disease. Consequently, we believe the proteomic approach could be useful both to define a panel of potential diagnostic biomarkers and to shed new light on the comprehension of the pathogenetic pathways of CFS.</jats:p> </jats:sec> A multidisciplinary approach to study a couple of monozygotic twins discordant for the chronic fatigue syndrome: a focus on potential salivary biomarkers Journal of Translational Medicine |
| spellingShingle | Ciregia, Federica, Giusti, Laura, Da Valle, Ylenia, Donadio, Elena, Consensi, Arianna, Giacomelli, Camillo, Sernissi, Francesca, Scarpellini, Pietro, Maggi, Fabrizio, Lucacchini, Antonio, Bazzichi, Laura, Journal of Translational Medicine, A multidisciplinary approach to study a couple of monozygotic twins discordant for the chronic fatigue syndrome: a focus on potential salivary biomarkers, General Biochemistry, Genetics and Molecular Biology, General Medicine |
| title | A multidisciplinary approach to study a couple of monozygotic twins discordant for the chronic fatigue syndrome: a focus on potential salivary biomarkers |
| title_full | A multidisciplinary approach to study a couple of monozygotic twins discordant for the chronic fatigue syndrome: a focus on potential salivary biomarkers |
| title_fullStr | A multidisciplinary approach to study a couple of monozygotic twins discordant for the chronic fatigue syndrome: a focus on potential salivary biomarkers |
| title_full_unstemmed | A multidisciplinary approach to study a couple of monozygotic twins discordant for the chronic fatigue syndrome: a focus on potential salivary biomarkers |
| title_short | A multidisciplinary approach to study a couple of monozygotic twins discordant for the chronic fatigue syndrome: a focus on potential salivary biomarkers |
| title_sort | a multidisciplinary approach to study a couple of monozygotic twins discordant for the chronic fatigue syndrome: a focus on potential salivary biomarkers |
| title_unstemmed | A multidisciplinary approach to study a couple of monozygotic twins discordant for the chronic fatigue syndrome: a focus on potential salivary biomarkers |
| topic | General Biochemistry, Genetics and Molecular Biology, General Medicine |
| url | http://dx.doi.org/10.1186/1479-5876-11-243 |