Eintrag weiter verarbeiten
Copper-transporting P-type adenosine triphosphatase (ATP7A) is associated with platinum-resistance in non-small cell lung cancer (NSCLC)
Gespeichert in:
| Zeitschriftentitel: | Journal of Translational Medicine |
|---|---|
| Personen und Körperschaften: | , , , , , , , , , |
| In: | Journal of Translational Medicine, 10, 2012, 1 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Springer Science and Business Media LLC
|
| Schlagwörter: |
| author_facet |
Li, Zhuang-hua Qiu, Miao-zhen Zeng, Zhao-lei Luo, Hui-yan Wu, Wen-jing Wang, Feng Wang, Zhi-qiang Zhang, Dong-sheng Li, Yu-hong Xu, Rui-hua Li, Zhuang-hua Qiu, Miao-zhen Zeng, Zhao-lei Luo, Hui-yan Wu, Wen-jing Wang, Feng Wang, Zhi-qiang Zhang, Dong-sheng Li, Yu-hong Xu, Rui-hua |
|---|---|
| author |
Li, Zhuang-hua Qiu, Miao-zhen Zeng, Zhao-lei Luo, Hui-yan Wu, Wen-jing Wang, Feng Wang, Zhi-qiang Zhang, Dong-sheng Li, Yu-hong Xu, Rui-hua |
| spellingShingle |
Li, Zhuang-hua Qiu, Miao-zhen Zeng, Zhao-lei Luo, Hui-yan Wu, Wen-jing Wang, Feng Wang, Zhi-qiang Zhang, Dong-sheng Li, Yu-hong Xu, Rui-hua Journal of Translational Medicine Copper-transporting P-type adenosine triphosphatase (ATP7A) is associated with platinum-resistance in non-small cell lung cancer (NSCLC) General Biochemistry, Genetics and Molecular Biology General Medicine |
| author_sort |
li, zhuang-hua |
| spelling |
Li, Zhuang-hua Qiu, Miao-zhen Zeng, Zhao-lei Luo, Hui-yan Wu, Wen-jing Wang, Feng Wang, Zhi-qiang Zhang, Dong-sheng Li, Yu-hong Xu, Rui-hua 1479-5876 Springer Science and Business Media LLC General Biochemistry, Genetics and Molecular Biology General Medicine http://dx.doi.org/10.1186/1479-5876-10-21 <jats:title>Abstract</jats:title> <jats:sec> <jats:title>Background</jats:title> <jats:p>Copper export protein ATP7A is important for maintaining copper homeostasis. Recent studies have shown that copper transporters are also involved in the transport of platinum. The goal of this study was to determine the role of ATP7A in the platinum-resistance of non-small cell lung cancer (NSCLC).</jats:p> </jats:sec> <jats:sec> <jats:title>Methods</jats:title> <jats:p>Sensitivities to platinums were detected by MTT assay and drug-resistance related genes were analyzed by real-time PCR and immunoblotting between DDP-sensitive A549 and the corresponding DDP-resistant cell subline (A549/DDP). ATP7A expression was evaluated by immunohistochemistry in tumor tissues of unresectable NSCLC patients who received cisplatin-basing chemotherapy.</jats:p> </jats:sec> <jats:sec> <jats:title>Results</jats:title> <jats:p>The expression of ATP7A was significantly higher in A549/DDP cell subline than in A549 cells at both mRNA and protein levels. The silencing of ATP7A expression in A549/DDP by siRNA partially reversed DDP-resistance (29.62%) and increased cell apoptosis. ATP7A expression was detected in 41.6%of NSCLC patients, but not in adjacent stroma nor normal lung tissues. ATP7A-positive patients had a significantly poorer histological grade (p = 0.039) and poorer response to platinum-basing chemotherapy (p = 0.001) compared with ATP7A-negative patients. Cox's proportional hazards analysis showed that ATP7A expression was an independent prognostic factor for overall survival (p = 0.045).</jats:p> </jats:sec> <jats:sec> <jats:title>Conclusions</jats:title> <jats:p>ATP7A overexpression played an important role in platinum-resistance of NSCLC, and was a negative prognostic factor of NSCLC patients treated with platinum-based chemotherapy.</jats:p> </jats:sec> Copper-transporting P-type adenosine triphosphatase (ATP7A) is associated with platinum-resistance in non-small cell lung cancer (NSCLC) Journal of Translational Medicine |
| doi_str_mv |
10.1186/1479-5876-10-21 |
| facet_avail |
Online Free |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTE4Ni8xNDc5LTU4NzYtMTAtMjE |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTE4Ni8xNDc5LTU4NzYtMTAtMjE |
| institution |
DE-Brt1 DE-Zwi2 DE-D161 DE-Gla1 DE-Zi4 DE-15 DE-Rs1 DE-Pl11 DE-105 DE-14 DE-Ch1 DE-L229 DE-D275 DE-Bn3 |
| imprint |
Springer Science and Business Media LLC, 2012 |
| imprint_str_mv |
Springer Science and Business Media LLC, 2012 |
| issn |
1479-5876 |
| issn_str_mv |
1479-5876 |
| language |
English |
| mega_collection |
Springer Science and Business Media LLC (CrossRef) |
| match_str |
li2012coppertransportingptypeadenosinetriphosphataseatp7aisassociatedwithplatinumresistanceinnonsmallcelllungcancernsclc |
| publishDateSort |
2012 |
| publisher |
Springer Science and Business Media LLC |
| recordtype |
ai |
| record_format |
ai |
| series |
Journal of Translational Medicine |
| source_id |
49 |
| title |
Copper-transporting P-type adenosine triphosphatase (ATP7A) is associated with platinum-resistance in non-small cell lung cancer (NSCLC) |
| title_unstemmed |
Copper-transporting P-type adenosine triphosphatase (ATP7A) is associated with platinum-resistance in non-small cell lung cancer (NSCLC) |
| title_full |
Copper-transporting P-type adenosine triphosphatase (ATP7A) is associated with platinum-resistance in non-small cell lung cancer (NSCLC) |
| title_fullStr |
Copper-transporting P-type adenosine triphosphatase (ATP7A) is associated with platinum-resistance in non-small cell lung cancer (NSCLC) |
| title_full_unstemmed |
Copper-transporting P-type adenosine triphosphatase (ATP7A) is associated with platinum-resistance in non-small cell lung cancer (NSCLC) |
| title_short |
Copper-transporting P-type adenosine triphosphatase (ATP7A) is associated with platinum-resistance in non-small cell lung cancer (NSCLC) |
| title_sort |
copper-transporting p-type adenosine triphosphatase (atp7a) is associated with platinum-resistance in non-small cell lung cancer (nsclc) |
| topic |
General Biochemistry, Genetics and Molecular Biology General Medicine |
| url |
http://dx.doi.org/10.1186/1479-5876-10-21 |
| publishDate |
2012 |
| physical |
|
| description |
<jats:title>Abstract</jats:title>
<jats:sec>
<jats:title>Background</jats:title>
<jats:p>Copper export protein ATP7A is important for maintaining copper homeostasis. Recent studies have shown that copper transporters are also involved in the transport of platinum. The goal of this study was to determine the role of ATP7A in the platinum-resistance of non-small cell lung cancer (NSCLC).</jats:p>
</jats:sec>
<jats:sec>
<jats:title>Methods</jats:title>
<jats:p>Sensitivities to platinums were detected by MTT assay and drug-resistance related genes were analyzed by real-time PCR and immunoblotting between DDP-sensitive A549 and the corresponding DDP-resistant cell subline (A549/DDP). ATP7A expression was evaluated by immunohistochemistry in tumor tissues of unresectable NSCLC patients who received cisplatin-basing chemotherapy.</jats:p>
</jats:sec>
<jats:sec>
<jats:title>Results</jats:title>
<jats:p>The expression of ATP7A was significantly higher in A549/DDP cell subline than in A549 cells at both mRNA and protein levels. The silencing of ATP7A expression in A549/DDP by siRNA partially reversed DDP-resistance (29.62%) and increased cell apoptosis. ATP7A expression was detected in 41.6%of NSCLC patients, but not in adjacent stroma nor normal lung tissues. ATP7A-positive patients had a significantly poorer histological grade (p = 0.039) and poorer response to platinum-basing chemotherapy (p = 0.001) compared with ATP7A-negative patients. Cox's proportional hazards analysis showed that ATP7A expression was an independent prognostic factor for overall survival (p = 0.045).</jats:p>
</jats:sec>
<jats:sec>
<jats:title>Conclusions</jats:title>
<jats:p>ATP7A overexpression played an important role in platinum-resistance of NSCLC, and was a negative prognostic factor of NSCLC patients treated with platinum-based chemotherapy.</jats:p>
</jats:sec> |
| container_issue |
1 |
| container_start_page |
0 |
| container_title |
Journal of Translational Medicine |
| container_volume |
10 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792345825179860997 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T17:29:38.444Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Copper-transporting+P-type+adenosine+triphosphatase+%28ATP7A%29+is+associated+with+platinum-resistance+in+non-small+cell+lung+cancer+%28NSCLC%29&rft.date=2012-12-01&genre=article&issn=1479-5876&volume=10&issue=1&jtitle=Journal+of+Translational+Medicine&atitle=Copper-transporting+P-type+adenosine+triphosphatase+%28ATP7A%29+is+associated+with+platinum-resistance+in+non-small+cell+lung+cancer+%28NSCLC%29&aulast=Xu&aufirst=Rui-hua&rft_id=info%3Adoi%2F10.1186%2F1479-5876-10-21&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792345825179860997 |
| author | Li, Zhuang-hua, Qiu, Miao-zhen, Zeng, Zhao-lei, Luo, Hui-yan, Wu, Wen-jing, Wang, Feng, Wang, Zhi-qiang, Zhang, Dong-sheng, Li, Yu-hong, Xu, Rui-hua |
| author_facet | Li, Zhuang-hua, Qiu, Miao-zhen, Zeng, Zhao-lei, Luo, Hui-yan, Wu, Wen-jing, Wang, Feng, Wang, Zhi-qiang, Zhang, Dong-sheng, Li, Yu-hong, Xu, Rui-hua, Li, Zhuang-hua, Qiu, Miao-zhen, Zeng, Zhao-lei, Luo, Hui-yan, Wu, Wen-jing, Wang, Feng, Wang, Zhi-qiang, Zhang, Dong-sheng, Li, Yu-hong, Xu, Rui-hua |
| author_sort | li, zhuang-hua |
| container_issue | 1 |
| container_start_page | 0 |
| container_title | Journal of Translational Medicine |
| container_volume | 10 |
| description | <jats:title>Abstract</jats:title> <jats:sec> <jats:title>Background</jats:title> <jats:p>Copper export protein ATP7A is important for maintaining copper homeostasis. Recent studies have shown that copper transporters are also involved in the transport of platinum. The goal of this study was to determine the role of ATP7A in the platinum-resistance of non-small cell lung cancer (NSCLC).</jats:p> </jats:sec> <jats:sec> <jats:title>Methods</jats:title> <jats:p>Sensitivities to platinums were detected by MTT assay and drug-resistance related genes were analyzed by real-time PCR and immunoblotting between DDP-sensitive A549 and the corresponding DDP-resistant cell subline (A549/DDP). ATP7A expression was evaluated by immunohistochemistry in tumor tissues of unresectable NSCLC patients who received cisplatin-basing chemotherapy.</jats:p> </jats:sec> <jats:sec> <jats:title>Results</jats:title> <jats:p>The expression of ATP7A was significantly higher in A549/DDP cell subline than in A549 cells at both mRNA and protein levels. The silencing of ATP7A expression in A549/DDP by siRNA partially reversed DDP-resistance (29.62%) and increased cell apoptosis. ATP7A expression was detected in 41.6%of NSCLC patients, but not in adjacent stroma nor normal lung tissues. ATP7A-positive patients had a significantly poorer histological grade (p = 0.039) and poorer response to platinum-basing chemotherapy (p = 0.001) compared with ATP7A-negative patients. Cox's proportional hazards analysis showed that ATP7A expression was an independent prognostic factor for overall survival (p = 0.045).</jats:p> </jats:sec> <jats:sec> <jats:title>Conclusions</jats:title> <jats:p>ATP7A overexpression played an important role in platinum-resistance of NSCLC, and was a negative prognostic factor of NSCLC patients treated with platinum-based chemotherapy.</jats:p> </jats:sec> |
| doi_str_mv | 10.1186/1479-5876-10-21 |
| facet_avail | Online, Free |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTE4Ni8xNDc5LTU4NzYtMTAtMjE |
| imprint | Springer Science and Business Media LLC, 2012 |
| imprint_str_mv | Springer Science and Business Media LLC, 2012 |
| institution | DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Rs1, DE-Pl11, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3 |
| issn | 1479-5876 |
| issn_str_mv | 1479-5876 |
| language | English |
| last_indexed | 2024-03-01T17:29:38.444Z |
| match_str | li2012coppertransportingptypeadenosinetriphosphataseatp7aisassociatedwithplatinumresistanceinnonsmallcelllungcancernsclc |
| mega_collection | Springer Science and Business Media LLC (CrossRef) |
| physical | |
| publishDate | 2012 |
| publishDateSort | 2012 |
| publisher | Springer Science and Business Media LLC |
| record_format | ai |
| recordtype | ai |
| series | Journal of Translational Medicine |
| source_id | 49 |
| spelling | Li, Zhuang-hua Qiu, Miao-zhen Zeng, Zhao-lei Luo, Hui-yan Wu, Wen-jing Wang, Feng Wang, Zhi-qiang Zhang, Dong-sheng Li, Yu-hong Xu, Rui-hua 1479-5876 Springer Science and Business Media LLC General Biochemistry, Genetics and Molecular Biology General Medicine http://dx.doi.org/10.1186/1479-5876-10-21 <jats:title>Abstract</jats:title> <jats:sec> <jats:title>Background</jats:title> <jats:p>Copper export protein ATP7A is important for maintaining copper homeostasis. Recent studies have shown that copper transporters are also involved in the transport of platinum. The goal of this study was to determine the role of ATP7A in the platinum-resistance of non-small cell lung cancer (NSCLC).</jats:p> </jats:sec> <jats:sec> <jats:title>Methods</jats:title> <jats:p>Sensitivities to platinums were detected by MTT assay and drug-resistance related genes were analyzed by real-time PCR and immunoblotting between DDP-sensitive A549 and the corresponding DDP-resistant cell subline (A549/DDP). ATP7A expression was evaluated by immunohistochemistry in tumor tissues of unresectable NSCLC patients who received cisplatin-basing chemotherapy.</jats:p> </jats:sec> <jats:sec> <jats:title>Results</jats:title> <jats:p>The expression of ATP7A was significantly higher in A549/DDP cell subline than in A549 cells at both mRNA and protein levels. The silencing of ATP7A expression in A549/DDP by siRNA partially reversed DDP-resistance (29.62%) and increased cell apoptosis. ATP7A expression was detected in 41.6%of NSCLC patients, but not in adjacent stroma nor normal lung tissues. ATP7A-positive patients had a significantly poorer histological grade (p = 0.039) and poorer response to platinum-basing chemotherapy (p = 0.001) compared with ATP7A-negative patients. Cox's proportional hazards analysis showed that ATP7A expression was an independent prognostic factor for overall survival (p = 0.045).</jats:p> </jats:sec> <jats:sec> <jats:title>Conclusions</jats:title> <jats:p>ATP7A overexpression played an important role in platinum-resistance of NSCLC, and was a negative prognostic factor of NSCLC patients treated with platinum-based chemotherapy.</jats:p> </jats:sec> Copper-transporting P-type adenosine triphosphatase (ATP7A) is associated with platinum-resistance in non-small cell lung cancer (NSCLC) Journal of Translational Medicine |
| spellingShingle | Li, Zhuang-hua, Qiu, Miao-zhen, Zeng, Zhao-lei, Luo, Hui-yan, Wu, Wen-jing, Wang, Feng, Wang, Zhi-qiang, Zhang, Dong-sheng, Li, Yu-hong, Xu, Rui-hua, Journal of Translational Medicine, Copper-transporting P-type adenosine triphosphatase (ATP7A) is associated with platinum-resistance in non-small cell lung cancer (NSCLC), General Biochemistry, Genetics and Molecular Biology, General Medicine |
| title | Copper-transporting P-type adenosine triphosphatase (ATP7A) is associated with platinum-resistance in non-small cell lung cancer (NSCLC) |
| title_full | Copper-transporting P-type adenosine triphosphatase (ATP7A) is associated with platinum-resistance in non-small cell lung cancer (NSCLC) |
| title_fullStr | Copper-transporting P-type adenosine triphosphatase (ATP7A) is associated with platinum-resistance in non-small cell lung cancer (NSCLC) |
| title_full_unstemmed | Copper-transporting P-type adenosine triphosphatase (ATP7A) is associated with platinum-resistance in non-small cell lung cancer (NSCLC) |
| title_short | Copper-transporting P-type adenosine triphosphatase (ATP7A) is associated with platinum-resistance in non-small cell lung cancer (NSCLC) |
| title_sort | copper-transporting p-type adenosine triphosphatase (atp7a) is associated with platinum-resistance in non-small cell lung cancer (nsclc) |
| title_unstemmed | Copper-transporting P-type adenosine triphosphatase (ATP7A) is associated with platinum-resistance in non-small cell lung cancer (NSCLC) |
| topic | General Biochemistry, Genetics and Molecular Biology, General Medicine |
| url | http://dx.doi.org/10.1186/1479-5876-10-21 |