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Regulatory elements of the EKLF gene that direct erythroid cell-specific expression during mammalian development
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| Zeitschriftentitel: | Blood |
|---|---|
| Personen und Körperschaften: | , , , |
| In: | Blood, 103, 2004, 11, S. 4078-4083 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society of Hematology
|
| Schlagwörter: |
| author_facet |
Xue, Li Chen, Xiaoyong Chang, Yanjie Bieker, James J. Xue, Li Chen, Xiaoyong Chang, Yanjie Bieker, James J. |
|---|---|
| author |
Xue, Li Chen, Xiaoyong Chang, Yanjie Bieker, James J. |
| spellingShingle |
Xue, Li Chen, Xiaoyong Chang, Yanjie Bieker, James J. Blood Regulatory elements of the EKLF gene that direct erythroid cell-specific expression during mammalian development Cell Biology Hematology Immunology Biochemistry |
| author_sort |
xue, li |
| spelling |
Xue, Li Chen, Xiaoyong Chang, Yanjie Bieker, James J. 0006-4971 1528-0020 American Society of Hematology Cell Biology Hematology Immunology Biochemistry http://dx.doi.org/10.1182/blood-2003-09-3231 <jats:title>Abstract</jats:title><jats:p>Erythroid Krüppel-like factor (EKLF) plays an essential role in enabling β-globin expression during erythroid ontogeny. It is first expressed in the extraembryonic mesoderm of the yolk sac within the morphologically unique cells that give rise to the blood islands, and then later within the hepatic primordia. The BMP4/Smad pathway plays a critical role in the induction of EKLF, and transient transfection analyses demonstrate that sequences located within less than 1 kb of its transcription initiation site are sufficient for high-level erythroid-specific transcription. We have used transgenic analyses to verify that 950 bp located adjacent to the EKLF start site of transcription is sufficient to generate lacZ expression within the blood islands as well as the fetal liver during embryonic development. Of particular importance are 3 regions, 2 of which overlap endogenous erythroid-specific DNase hypersensitive sites, and 1 of which includes the proximal promoter region. The onset of transgene expression mimics that of endogenous EKLF as it begins by day 7.5 (d7.5) to d8.0. In addition, it exhibits a strict hematopoietic specificity, localized only to these cells and not to the adjacent vasculature at all stages examined. Finally, expression is heterocellular, implying that although these elements are sufficient for tissue-specific expression, they do not shield against the position effects of adjacent chromatin. These analyses demonstrate that a surprisingly small DNA segment contains all the information needed to target a linked gene to the hematopoietic compartment at both early and later stages of development, and may be a useful cassette for this purpose.</jats:p> Regulatory elements of the EKLF gene that direct erythroid cell-specific expression during mammalian development Blood |
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10.1182/blood-2003-09-3231 |
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American Society of Hematology, 2004 |
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American Society of Hematology, 2004 |
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Regulatory elements of the EKLF gene that direct erythroid cell-specific expression during mammalian development |
| title_unstemmed |
Regulatory elements of the EKLF gene that direct erythroid cell-specific expression during mammalian development |
| title_full |
Regulatory elements of the EKLF gene that direct erythroid cell-specific expression during mammalian development |
| title_fullStr |
Regulatory elements of the EKLF gene that direct erythroid cell-specific expression during mammalian development |
| title_full_unstemmed |
Regulatory elements of the EKLF gene that direct erythroid cell-specific expression during mammalian development |
| title_short |
Regulatory elements of the EKLF gene that direct erythroid cell-specific expression during mammalian development |
| title_sort |
regulatory elements of the eklf gene that direct erythroid cell-specific expression during mammalian development |
| topic |
Cell Biology Hematology Immunology Biochemistry |
| url |
http://dx.doi.org/10.1182/blood-2003-09-3231 |
| publishDate |
2004 |
| physical |
4078-4083 |
| description |
<jats:title>Abstract</jats:title><jats:p>Erythroid Krüppel-like factor (EKLF) plays an essential role in enabling β-globin expression during erythroid ontogeny. It is first expressed in the extraembryonic mesoderm of the yolk sac within the morphologically unique cells that give rise to the blood islands, and then later within the hepatic primordia. The BMP4/Smad pathway plays a critical role in the induction of EKLF, and transient transfection analyses demonstrate that sequences located within less than 1 kb of its transcription initiation site are sufficient for high-level erythroid-specific transcription. We have used transgenic analyses to verify that 950 bp located adjacent to the EKLF start site of transcription is sufficient to generate lacZ expression within the blood islands as well as the fetal liver during embryonic development. Of particular importance are 3 regions, 2 of which overlap endogenous erythroid-specific DNase hypersensitive sites, and 1 of which includes the proximal promoter region. The onset of transgene expression mimics that of endogenous EKLF as it begins by day 7.5 (d7.5) to d8.0. In addition, it exhibits a strict hematopoietic specificity, localized only to these cells and not to the adjacent vasculature at all stages examined. Finally, expression is heterocellular, implying that although these elements are sufficient for tissue-specific expression, they do not shield against the position effects of adjacent chromatin. These analyses demonstrate that a surprisingly small DNA segment contains all the information needed to target a linked gene to the hematopoietic compartment at both early and later stages of development, and may be a useful cassette for this purpose.</jats:p> |
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| author | Xue, Li, Chen, Xiaoyong, Chang, Yanjie, Bieker, James J. |
| author_facet | Xue, Li, Chen, Xiaoyong, Chang, Yanjie, Bieker, James J., Xue, Li, Chen, Xiaoyong, Chang, Yanjie, Bieker, James J. |
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| description | <jats:title>Abstract</jats:title><jats:p>Erythroid Krüppel-like factor (EKLF) plays an essential role in enabling β-globin expression during erythroid ontogeny. It is first expressed in the extraembryonic mesoderm of the yolk sac within the morphologically unique cells that give rise to the blood islands, and then later within the hepatic primordia. The BMP4/Smad pathway plays a critical role in the induction of EKLF, and transient transfection analyses demonstrate that sequences located within less than 1 kb of its transcription initiation site are sufficient for high-level erythroid-specific transcription. We have used transgenic analyses to verify that 950 bp located adjacent to the EKLF start site of transcription is sufficient to generate lacZ expression within the blood islands as well as the fetal liver during embryonic development. Of particular importance are 3 regions, 2 of which overlap endogenous erythroid-specific DNase hypersensitive sites, and 1 of which includes the proximal promoter region. The onset of transgene expression mimics that of endogenous EKLF as it begins by day 7.5 (d7.5) to d8.0. In addition, it exhibits a strict hematopoietic specificity, localized only to these cells and not to the adjacent vasculature at all stages examined. Finally, expression is heterocellular, implying that although these elements are sufficient for tissue-specific expression, they do not shield against the position effects of adjacent chromatin. These analyses demonstrate that a surprisingly small DNA segment contains all the information needed to target a linked gene to the hematopoietic compartment at both early and later stages of development, and may be a useful cassette for this purpose.</jats:p> |
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| imprint_str_mv | American Society of Hematology, 2004 |
| institution | DE-Zwi2, DE-D161, DE-Zi4, DE-Gla1, DE-15, DE-Pl11, DE-Rs1, DE-14, DE-105, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1 |
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| spelling | Xue, Li Chen, Xiaoyong Chang, Yanjie Bieker, James J. 0006-4971 1528-0020 American Society of Hematology Cell Biology Hematology Immunology Biochemistry http://dx.doi.org/10.1182/blood-2003-09-3231 <jats:title>Abstract</jats:title><jats:p>Erythroid Krüppel-like factor (EKLF) plays an essential role in enabling β-globin expression during erythroid ontogeny. It is first expressed in the extraembryonic mesoderm of the yolk sac within the morphologically unique cells that give rise to the blood islands, and then later within the hepatic primordia. The BMP4/Smad pathway plays a critical role in the induction of EKLF, and transient transfection analyses demonstrate that sequences located within less than 1 kb of its transcription initiation site are sufficient for high-level erythroid-specific transcription. We have used transgenic analyses to verify that 950 bp located adjacent to the EKLF start site of transcription is sufficient to generate lacZ expression within the blood islands as well as the fetal liver during embryonic development. Of particular importance are 3 regions, 2 of which overlap endogenous erythroid-specific DNase hypersensitive sites, and 1 of which includes the proximal promoter region. The onset of transgene expression mimics that of endogenous EKLF as it begins by day 7.5 (d7.5) to d8.0. In addition, it exhibits a strict hematopoietic specificity, localized only to these cells and not to the adjacent vasculature at all stages examined. Finally, expression is heterocellular, implying that although these elements are sufficient for tissue-specific expression, they do not shield against the position effects of adjacent chromatin. These analyses demonstrate that a surprisingly small DNA segment contains all the information needed to target a linked gene to the hematopoietic compartment at both early and later stages of development, and may be a useful cassette for this purpose.</jats:p> Regulatory elements of the EKLF gene that direct erythroid cell-specific expression during mammalian development Blood |
| spellingShingle | Xue, Li, Chen, Xiaoyong, Chang, Yanjie, Bieker, James J., Blood, Regulatory elements of the EKLF gene that direct erythroid cell-specific expression during mammalian development, Cell Biology, Hematology, Immunology, Biochemistry |
| title | Regulatory elements of the EKLF gene that direct erythroid cell-specific expression during mammalian development |
| title_full | Regulatory elements of the EKLF gene that direct erythroid cell-specific expression during mammalian development |
| title_fullStr | Regulatory elements of the EKLF gene that direct erythroid cell-specific expression during mammalian development |
| title_full_unstemmed | Regulatory elements of the EKLF gene that direct erythroid cell-specific expression during mammalian development |
| title_short | Regulatory elements of the EKLF gene that direct erythroid cell-specific expression during mammalian development |
| title_sort | regulatory elements of the eklf gene that direct erythroid cell-specific expression during mammalian development |
| title_unstemmed | Regulatory elements of the EKLF gene that direct erythroid cell-specific expression during mammalian development |
| topic | Cell Biology, Hematology, Immunology, Biochemistry |
| url | http://dx.doi.org/10.1182/blood-2003-09-3231 |