Eintrag weiter verarbeiten
Revealing lymphoma growth and the efficacy of immune cell therapies using in vivo bioluminescence imaging
Gespeichert in:
| Zeitschriftentitel: | Blood |
|---|---|
| Personen und Körperschaften: | , , , , , |
| In: | Blood, 101, 2003, 2, S. 640-648 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society of Hematology
|
| Schlagwörter: |
| author_facet |
Edinger, Matthias Cao, Yu-An Verneris, Michael R. Bachmann, Michael H. Contag, Christopher H. Negrin, Robert S. Edinger, Matthias Cao, Yu-An Verneris, Michael R. Bachmann, Michael H. Contag, Christopher H. Negrin, Robert S. |
|---|---|
| author |
Edinger, Matthias Cao, Yu-An Verneris, Michael R. Bachmann, Michael H. Contag, Christopher H. Negrin, Robert S. |
| spellingShingle |
Edinger, Matthias Cao, Yu-An Verneris, Michael R. Bachmann, Michael H. Contag, Christopher H. Negrin, Robert S. Blood Revealing lymphoma growth and the efficacy of immune cell therapies using in vivo bioluminescence imaging Cell Biology Hematology Immunology Biochemistry |
| author_sort |
edinger, matthias |
| spelling |
Edinger, Matthias Cao, Yu-An Verneris, Michael R. Bachmann, Michael H. Contag, Christopher H. Negrin, Robert S. 1528-0020 0006-4971 American Society of Hematology Cell Biology Hematology Immunology Biochemistry http://dx.doi.org/10.1182/blood-2002-06-1751 <jats:p>Cancer therapeutics have achieved success in the treatment of a variety of malignancies, however, relapse of disease from small numbers of persistent tumor cells remains a major obstacle. Advancement of treatment regimens that effectively control minimal residual disease and prevent relapse would be greatly accelerated if sensitive and noninvasive assays were used to quantitatively assess tumor burden in animal models of minimal residual disease that are predictive of the human response. In vivo bioluminescence imaging (BLI) is an assay for the detection of small numbers of cells noninvasively and enables the quantification of tumor growth within internal organs. Fusion genes that encode bioluminescent and fluorescent reporter proteins effectively couple the powerful in vivo capabilities of BLI with the subset-discriminating capabilities of fluorescence-activated cell sorting. We labeled 2 murine lymphoma cell lines with dual function reporter genes and monitored radiation and chemotherapy as well as immune-based strategies that employ the tumorcidal activity of ex vivo–expanded CD8+ natural killer (NK)–T cells. Using BLI we were able to visualize the entire course of malignant disease including engraftment, expansion, metastasis, response to therapy, and unique patterns of relapse. We also labeled the effector NK-T cells and monitored their homing to the sites of tumor growth followed by tumor eradication. These studies reveal the efficacy of immune cell therapies and the tempo of NK-T cell trafficking in vivo. The complex cellular processes in bone marrow transplantation and antitumor immunotherapy, previously inaccessible to investigation, can now be revealed in real time in living animals.</jats:p> Revealing lymphoma growth and the efficacy of immune cell therapies using in vivo bioluminescence imaging Blood |
| doi_str_mv |
10.1182/blood-2002-06-1751 |
| facet_avail |
Online Free |
| finc_class_facet |
Chemie und Pharmazie Biologie Medizin |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTE4Mi9ibG9vZC0yMDAyLTA2LTE3NTE |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTE4Mi9ibG9vZC0yMDAyLTA2LTE3NTE |
| institution |
DE-Zi4 DE-Gla1 DE-15 DE-Pl11 DE-Rs1 DE-14 DE-105 DE-Ch1 DE-L229 DE-D275 DE-Bn3 DE-Brt1 DE-Zwi2 DE-D161 |
| imprint |
American Society of Hematology, 2003 |
| imprint_str_mv |
American Society of Hematology, 2003 |
| issn |
1528-0020 0006-4971 |
| issn_str_mv |
1528-0020 0006-4971 |
| language |
English |
| mega_collection |
American Society of Hematology (CrossRef) |
| match_str |
edinger2003revealinglymphomagrowthandtheefficacyofimmunecelltherapiesusinginvivobioluminescenceimaging |
| publishDateSort |
2003 |
| publisher |
American Society of Hematology |
| recordtype |
ai |
| record_format |
ai |
| series |
Blood |
| source_id |
49 |
| title |
Revealing lymphoma growth and the efficacy of immune cell therapies using in vivo bioluminescence imaging |
| title_unstemmed |
Revealing lymphoma growth and the efficacy of immune cell therapies using in vivo bioluminescence imaging |
| title_full |
Revealing lymphoma growth and the efficacy of immune cell therapies using in vivo bioluminescence imaging |
| title_fullStr |
Revealing lymphoma growth and the efficacy of immune cell therapies using in vivo bioluminescence imaging |
| title_full_unstemmed |
Revealing lymphoma growth and the efficacy of immune cell therapies using in vivo bioluminescence imaging |
| title_short |
Revealing lymphoma growth and the efficacy of immune cell therapies using in vivo bioluminescence imaging |
| title_sort |
revealing lymphoma growth and the efficacy of immune cell therapies using in vivo bioluminescence imaging |
| topic |
Cell Biology Hematology Immunology Biochemistry |
| url |
http://dx.doi.org/10.1182/blood-2002-06-1751 |
| publishDate |
2003 |
| physical |
640-648 |
| description |
<jats:p>Cancer therapeutics have achieved success in the treatment of a variety of malignancies, however, relapse of disease from small numbers of persistent tumor cells remains a major obstacle. Advancement of treatment regimens that effectively control minimal residual disease and prevent relapse would be greatly accelerated if sensitive and noninvasive assays were used to quantitatively assess tumor burden in animal models of minimal residual disease that are predictive of the human response. In vivo bioluminescence imaging (BLI) is an assay for the detection of small numbers of cells noninvasively and enables the quantification of tumor growth within internal organs. Fusion genes that encode bioluminescent and fluorescent reporter proteins effectively couple the powerful in vivo capabilities of BLI with the subset-discriminating capabilities of fluorescence-activated cell sorting. We labeled 2 murine lymphoma cell lines with dual function reporter genes and monitored radiation and chemotherapy as well as immune-based strategies that employ the tumorcidal activity of ex vivo–expanded CD8+ natural killer (NK)–T cells. Using BLI we were able to visualize the entire course of malignant disease including engraftment, expansion, metastasis, response to therapy, and unique patterns of relapse. We also labeled the effector NK-T cells and monitored their homing to the sites of tumor growth followed by tumor eradication. These studies reveal the efficacy of immune cell therapies and the tempo of NK-T cell trafficking in vivo. The complex cellular processes in bone marrow transplantation and antitumor immunotherapy, previously inaccessible to investigation, can now be revealed in real time in living animals.</jats:p> |
| container_issue |
2 |
| container_start_page |
640 |
| container_title |
Blood |
| container_volume |
101 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792346306220392451 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T17:37:18.214Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Revealing+lymphoma+growth+and+the+efficacy+of+immune+cell+therapies+using+in+vivo+bioluminescence+imaging&rft.date=2003-01-15&genre=article&issn=0006-4971&volume=101&issue=2&spage=640&epage=648&pages=640-648&jtitle=Blood&atitle=Revealing+lymphoma+growth+and+the+efficacy+of+immune+cell+therapies+using+in+vivo+bioluminescence+imaging&aulast=Negrin&aufirst=Robert+S.&rft_id=info%3Adoi%2F10.1182%2Fblood-2002-06-1751&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792346306220392451 |
| author | Edinger, Matthias, Cao, Yu-An, Verneris, Michael R., Bachmann, Michael H., Contag, Christopher H., Negrin, Robert S. |
| author_facet | Edinger, Matthias, Cao, Yu-An, Verneris, Michael R., Bachmann, Michael H., Contag, Christopher H., Negrin, Robert S., Edinger, Matthias, Cao, Yu-An, Verneris, Michael R., Bachmann, Michael H., Contag, Christopher H., Negrin, Robert S. |
| author_sort | edinger, matthias |
| container_issue | 2 |
| container_start_page | 640 |
| container_title | Blood |
| container_volume | 101 |
| description | <jats:p>Cancer therapeutics have achieved success in the treatment of a variety of malignancies, however, relapse of disease from small numbers of persistent tumor cells remains a major obstacle. Advancement of treatment regimens that effectively control minimal residual disease and prevent relapse would be greatly accelerated if sensitive and noninvasive assays were used to quantitatively assess tumor burden in animal models of minimal residual disease that are predictive of the human response. In vivo bioluminescence imaging (BLI) is an assay for the detection of small numbers of cells noninvasively and enables the quantification of tumor growth within internal organs. Fusion genes that encode bioluminescent and fluorescent reporter proteins effectively couple the powerful in vivo capabilities of BLI with the subset-discriminating capabilities of fluorescence-activated cell sorting. We labeled 2 murine lymphoma cell lines with dual function reporter genes and monitored radiation and chemotherapy as well as immune-based strategies that employ the tumorcidal activity of ex vivo–expanded CD8+ natural killer (NK)–T cells. Using BLI we were able to visualize the entire course of malignant disease including engraftment, expansion, metastasis, response to therapy, and unique patterns of relapse. We also labeled the effector NK-T cells and monitored their homing to the sites of tumor growth followed by tumor eradication. These studies reveal the efficacy of immune cell therapies and the tempo of NK-T cell trafficking in vivo. The complex cellular processes in bone marrow transplantation and antitumor immunotherapy, previously inaccessible to investigation, can now be revealed in real time in living animals.</jats:p> |
| doi_str_mv | 10.1182/blood-2002-06-1751 |
| facet_avail | Online, Free |
| finc_class_facet | Chemie und Pharmazie, Biologie, Medizin |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTE4Mi9ibG9vZC0yMDAyLTA2LTE3NTE |
| imprint | American Society of Hematology, 2003 |
| imprint_str_mv | American Society of Hematology, 2003 |
| institution | DE-Zi4, DE-Gla1, DE-15, DE-Pl11, DE-Rs1, DE-14, DE-105, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161 |
| issn | 1528-0020, 0006-4971 |
| issn_str_mv | 1528-0020, 0006-4971 |
| language | English |
| last_indexed | 2024-03-01T17:37:18.214Z |
| match_str | edinger2003revealinglymphomagrowthandtheefficacyofimmunecelltherapiesusinginvivobioluminescenceimaging |
| mega_collection | American Society of Hematology (CrossRef) |
| physical | 640-648 |
| publishDate | 2003 |
| publishDateSort | 2003 |
| publisher | American Society of Hematology |
| record_format | ai |
| recordtype | ai |
| series | Blood |
| source_id | 49 |
| spelling | Edinger, Matthias Cao, Yu-An Verneris, Michael R. Bachmann, Michael H. Contag, Christopher H. Negrin, Robert S. 1528-0020 0006-4971 American Society of Hematology Cell Biology Hematology Immunology Biochemistry http://dx.doi.org/10.1182/blood-2002-06-1751 <jats:p>Cancer therapeutics have achieved success in the treatment of a variety of malignancies, however, relapse of disease from small numbers of persistent tumor cells remains a major obstacle. Advancement of treatment regimens that effectively control minimal residual disease and prevent relapse would be greatly accelerated if sensitive and noninvasive assays were used to quantitatively assess tumor burden in animal models of minimal residual disease that are predictive of the human response. In vivo bioluminescence imaging (BLI) is an assay for the detection of small numbers of cells noninvasively and enables the quantification of tumor growth within internal organs. Fusion genes that encode bioluminescent and fluorescent reporter proteins effectively couple the powerful in vivo capabilities of BLI with the subset-discriminating capabilities of fluorescence-activated cell sorting. We labeled 2 murine lymphoma cell lines with dual function reporter genes and monitored radiation and chemotherapy as well as immune-based strategies that employ the tumorcidal activity of ex vivo–expanded CD8+ natural killer (NK)–T cells. Using BLI we were able to visualize the entire course of malignant disease including engraftment, expansion, metastasis, response to therapy, and unique patterns of relapse. We also labeled the effector NK-T cells and monitored their homing to the sites of tumor growth followed by tumor eradication. These studies reveal the efficacy of immune cell therapies and the tempo of NK-T cell trafficking in vivo. The complex cellular processes in bone marrow transplantation and antitumor immunotherapy, previously inaccessible to investigation, can now be revealed in real time in living animals.</jats:p> Revealing lymphoma growth and the efficacy of immune cell therapies using in vivo bioluminescence imaging Blood |
| spellingShingle | Edinger, Matthias, Cao, Yu-An, Verneris, Michael R., Bachmann, Michael H., Contag, Christopher H., Negrin, Robert S., Blood, Revealing lymphoma growth and the efficacy of immune cell therapies using in vivo bioluminescence imaging, Cell Biology, Hematology, Immunology, Biochemistry |
| title | Revealing lymphoma growth and the efficacy of immune cell therapies using in vivo bioluminescence imaging |
| title_full | Revealing lymphoma growth and the efficacy of immune cell therapies using in vivo bioluminescence imaging |
| title_fullStr | Revealing lymphoma growth and the efficacy of immune cell therapies using in vivo bioluminescence imaging |
| title_full_unstemmed | Revealing lymphoma growth and the efficacy of immune cell therapies using in vivo bioluminescence imaging |
| title_short | Revealing lymphoma growth and the efficacy of immune cell therapies using in vivo bioluminescence imaging |
| title_sort | revealing lymphoma growth and the efficacy of immune cell therapies using in vivo bioluminescence imaging |
| title_unstemmed | Revealing lymphoma growth and the efficacy of immune cell therapies using in vivo bioluminescence imaging |
| topic | Cell Biology, Hematology, Immunology, Biochemistry |
| url | http://dx.doi.org/10.1182/blood-2002-06-1751 |