Eintrag weiter verarbeiten
Elastase II Gene Encoding as Inflammatory Molecules in Acute Myeloblastic Leukemia
Gespeichert in:
| Zeitschriftentitel: | European Journal of Inflammation |
|---|---|
| Personen und Körperschaften: | , , , , , |
| In: | European Journal of Inflammation, 6, 2008, 2, S. 73-79 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
SAGE Publications
|
| Schlagwörter: |
| author_facet |
Ahangari, G. Shamsodin, N. Chavoshzadeh, Z. Moghadam, K.A. Ghavamzadeh, A. Nazarian, S.H. Ahangari, G. Shamsodin, N. Chavoshzadeh, Z. Moghadam, K.A. Ghavamzadeh, A. Nazarian, S.H. |
|---|---|
| author |
Ahangari, G. Shamsodin, N. Chavoshzadeh, Z. Moghadam, K.A. Ghavamzadeh, A. Nazarian, S.H. |
| spellingShingle |
Ahangari, G. Shamsodin, N. Chavoshzadeh, Z. Moghadam, K.A. Ghavamzadeh, A. Nazarian, S.H. European Journal of Inflammation Elastase II Gene Encoding as Inflammatory Molecules in Acute Myeloblastic Leukemia General Medicine Immunology Immunology and Allergy |
| author_sort |
ahangari, g. |
| spelling |
Ahangari, G. Shamsodin, N. Chavoshzadeh, Z. Moghadam, K.A. Ghavamzadeh, A. Nazarian, S.H. 2058-7392 2058-7392 SAGE Publications General Medicine Immunology Immunology and Allergy http://dx.doi.org/10.1177/1721727x0800600204 <jats:p> Acute myeloblastic leukemia (AML) is characterized by uncontrolled proliferation with the bone marrow (BM) of malignant myeloid progenitors arrested in their maturation process and the egress of these abnormal cells into the circulation. There is evidence that neutrophil production is a balance between the proliferative action of granulocyte-colony-stimulating factor (G-CSF) and a negative feedback from mature neutrophils. Recently, there have been reports on mutations in neutrophil elastase (ELA2) gene in genomic DNA of cyclic neutropenia. These patients developed acute myeloblastic leukemia. Therefore, we hypothesized that elastase may play role in the abnormal AML. Peripheral blood was obtained from 42 patients with acute myeloblastic leukemia and 30 healthy individuals. Total RNA was isolated using RNA standard techniques from freshly separated cells by polymorphoprep. RNA was analyzed by employing PCR amplification of reverse transcribed using a total of ten specific primers. We amplified five exons of ELA2 gene separately and sequenced each exon. Mutational analysis was carried out by directed capillary sequencing method. We found no mutation in 42 Acute myeloblastic leukemia patients compared to healthy individuals. Interestingly, we found heterozygote 50% single nucleotide polymorphism (SNP) in exon II codon 44 of healthy individuals but not in AML patients. It was a silent mutation G to A substitution but no changes in amino acid sequences. The codon sequence was GCG that changed to GCA. </jats:p> Elastase II Gene Encoding as Inflammatory Molecules in Acute Myeloblastic Leukemia European Journal of Inflammation |
| doi_str_mv |
10.1177/1721727x0800600204 |
| facet_avail |
Online Free |
| finc_class_facet |
Medizin |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTE3Ny8xNzIxNzI3eDA4MDA2MDAyMDQ |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTE3Ny8xNzIxNzI3eDA4MDA2MDAyMDQ |
| institution |
DE-Gla1 DE-Zi4 DE-15 DE-Rs1 DE-Pl11 DE-105 DE-14 DE-Ch1 DE-L229 DE-D275 DE-Bn3 DE-Brt1 DE-Zwi2 DE-D161 |
| imprint |
SAGE Publications, 2008 |
| imprint_str_mv |
SAGE Publications, 2008 |
| issn |
2058-7392 |
| issn_str_mv |
2058-7392 |
| language |
English |
| mega_collection |
SAGE Publications (CrossRef) |
| match_str |
ahangari2008elastaseiigeneencodingasinflammatorymoleculesinacutemyeloblasticleukemia |
| publishDateSort |
2008 |
| publisher |
SAGE Publications |
| recordtype |
ai |
| record_format |
ai |
| series |
European Journal of Inflammation |
| source_id |
49 |
| title |
Elastase II Gene Encoding as Inflammatory Molecules in Acute Myeloblastic Leukemia |
| title_unstemmed |
Elastase II Gene Encoding as Inflammatory Molecules in Acute Myeloblastic Leukemia |
| title_full |
Elastase II Gene Encoding as Inflammatory Molecules in Acute Myeloblastic Leukemia |
| title_fullStr |
Elastase II Gene Encoding as Inflammatory Molecules in Acute Myeloblastic Leukemia |
| title_full_unstemmed |
Elastase II Gene Encoding as Inflammatory Molecules in Acute Myeloblastic Leukemia |
| title_short |
Elastase II Gene Encoding as Inflammatory Molecules in Acute Myeloblastic Leukemia |
| title_sort |
elastase ii gene encoding as inflammatory molecules in acute myeloblastic leukemia |
| topic |
General Medicine Immunology Immunology and Allergy |
| url |
http://dx.doi.org/10.1177/1721727x0800600204 |
| publishDate |
2008 |
| physical |
73-79 |
| description |
<jats:p> Acute myeloblastic leukemia (AML) is characterized by uncontrolled proliferation with the bone marrow (BM) of malignant myeloid progenitors arrested in their maturation process and the egress of these abnormal cells into the circulation. There is evidence that neutrophil production is a balance between the proliferative action of granulocyte-colony-stimulating factor (G-CSF) and a negative feedback from mature neutrophils. Recently, there have been reports on mutations in neutrophil elastase (ELA2) gene in genomic DNA of cyclic neutropenia. These patients developed acute myeloblastic leukemia. Therefore, we hypothesized that elastase may play role in the abnormal AML. Peripheral blood was obtained from 42 patients with acute myeloblastic leukemia and 30 healthy individuals. Total RNA was isolated using RNA standard techniques from freshly separated cells by polymorphoprep. RNA was analyzed by employing PCR amplification of reverse transcribed using a total of ten specific primers. We amplified five exons of ELA2 gene separately and sequenced each exon. Mutational analysis was carried out by directed capillary sequencing method. We found no mutation in 42 Acute myeloblastic leukemia patients compared to healthy individuals. Interestingly, we found heterozygote 50% single nucleotide polymorphism (SNP) in exon II codon 44 of healthy individuals but not in AML patients. It was a silent mutation G to A substitution but no changes in amino acid sequences. The codon sequence was GCG that changed to GCA. </jats:p> |
| container_issue |
2 |
| container_start_page |
73 |
| container_title |
European Journal of Inflammation |
| container_volume |
6 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792322315122376708 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T11:15:55.417Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Elastase+II+Gene+Encoding+as+Inflammatory+Molecules+in+Acute+Myeloblastic+Leukemia&rft.date=2008-05-01&genre=article&issn=2058-7392&volume=6&issue=2&spage=73&epage=79&pages=73-79&jtitle=European+Journal+of+Inflammation&atitle=Elastase+II+Gene+Encoding+as+Inflammatory+Molecules+in+Acute+Myeloblastic+Leukemia&aulast=Nazarian&aufirst=S.H.&rft_id=info%3Adoi%2F10.1177%2F1721727x0800600204&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792322315122376708 |
| author | Ahangari, G., Shamsodin, N., Chavoshzadeh, Z., Moghadam, K.A., Ghavamzadeh, A., Nazarian, S.H. |
| author_facet | Ahangari, G., Shamsodin, N., Chavoshzadeh, Z., Moghadam, K.A., Ghavamzadeh, A., Nazarian, S.H., Ahangari, G., Shamsodin, N., Chavoshzadeh, Z., Moghadam, K.A., Ghavamzadeh, A., Nazarian, S.H. |
| author_sort | ahangari, g. |
| container_issue | 2 |
| container_start_page | 73 |
| container_title | European Journal of Inflammation |
| container_volume | 6 |
| description | <jats:p> Acute myeloblastic leukemia (AML) is characterized by uncontrolled proliferation with the bone marrow (BM) of malignant myeloid progenitors arrested in their maturation process and the egress of these abnormal cells into the circulation. There is evidence that neutrophil production is a balance between the proliferative action of granulocyte-colony-stimulating factor (G-CSF) and a negative feedback from mature neutrophils. Recently, there have been reports on mutations in neutrophil elastase (ELA2) gene in genomic DNA of cyclic neutropenia. These patients developed acute myeloblastic leukemia. Therefore, we hypothesized that elastase may play role in the abnormal AML. Peripheral blood was obtained from 42 patients with acute myeloblastic leukemia and 30 healthy individuals. Total RNA was isolated using RNA standard techniques from freshly separated cells by polymorphoprep. RNA was analyzed by employing PCR amplification of reverse transcribed using a total of ten specific primers. We amplified five exons of ELA2 gene separately and sequenced each exon. Mutational analysis was carried out by directed capillary sequencing method. We found no mutation in 42 Acute myeloblastic leukemia patients compared to healthy individuals. Interestingly, we found heterozygote 50% single nucleotide polymorphism (SNP) in exon II codon 44 of healthy individuals but not in AML patients. It was a silent mutation G to A substitution but no changes in amino acid sequences. The codon sequence was GCG that changed to GCA. </jats:p> |
| doi_str_mv | 10.1177/1721727x0800600204 |
| facet_avail | Online, Free |
| finc_class_facet | Medizin |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTE3Ny8xNzIxNzI3eDA4MDA2MDAyMDQ |
| imprint | SAGE Publications, 2008 |
| imprint_str_mv | SAGE Publications, 2008 |
| institution | DE-Gla1, DE-Zi4, DE-15, DE-Rs1, DE-Pl11, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161 |
| issn | 2058-7392 |
| issn_str_mv | 2058-7392 |
| language | English |
| last_indexed | 2024-03-01T11:15:55.417Z |
| match_str | ahangari2008elastaseiigeneencodingasinflammatorymoleculesinacutemyeloblasticleukemia |
| mega_collection | SAGE Publications (CrossRef) |
| physical | 73-79 |
| publishDate | 2008 |
| publishDateSort | 2008 |
| publisher | SAGE Publications |
| record_format | ai |
| recordtype | ai |
| series | European Journal of Inflammation |
| source_id | 49 |
| spelling | Ahangari, G. Shamsodin, N. Chavoshzadeh, Z. Moghadam, K.A. Ghavamzadeh, A. Nazarian, S.H. 2058-7392 2058-7392 SAGE Publications General Medicine Immunology Immunology and Allergy http://dx.doi.org/10.1177/1721727x0800600204 <jats:p> Acute myeloblastic leukemia (AML) is characterized by uncontrolled proliferation with the bone marrow (BM) of malignant myeloid progenitors arrested in their maturation process and the egress of these abnormal cells into the circulation. There is evidence that neutrophil production is a balance between the proliferative action of granulocyte-colony-stimulating factor (G-CSF) and a negative feedback from mature neutrophils. Recently, there have been reports on mutations in neutrophil elastase (ELA2) gene in genomic DNA of cyclic neutropenia. These patients developed acute myeloblastic leukemia. Therefore, we hypothesized that elastase may play role in the abnormal AML. Peripheral blood was obtained from 42 patients with acute myeloblastic leukemia and 30 healthy individuals. Total RNA was isolated using RNA standard techniques from freshly separated cells by polymorphoprep. RNA was analyzed by employing PCR amplification of reverse transcribed using a total of ten specific primers. We amplified five exons of ELA2 gene separately and sequenced each exon. Mutational analysis was carried out by directed capillary sequencing method. We found no mutation in 42 Acute myeloblastic leukemia patients compared to healthy individuals. Interestingly, we found heterozygote 50% single nucleotide polymorphism (SNP) in exon II codon 44 of healthy individuals but not in AML patients. It was a silent mutation G to A substitution but no changes in amino acid sequences. The codon sequence was GCG that changed to GCA. </jats:p> Elastase II Gene Encoding as Inflammatory Molecules in Acute Myeloblastic Leukemia European Journal of Inflammation |
| spellingShingle | Ahangari, G., Shamsodin, N., Chavoshzadeh, Z., Moghadam, K.A., Ghavamzadeh, A., Nazarian, S.H., European Journal of Inflammation, Elastase II Gene Encoding as Inflammatory Molecules in Acute Myeloblastic Leukemia, General Medicine, Immunology, Immunology and Allergy |
| title | Elastase II Gene Encoding as Inflammatory Molecules in Acute Myeloblastic Leukemia |
| title_full | Elastase II Gene Encoding as Inflammatory Molecules in Acute Myeloblastic Leukemia |
| title_fullStr | Elastase II Gene Encoding as Inflammatory Molecules in Acute Myeloblastic Leukemia |
| title_full_unstemmed | Elastase II Gene Encoding as Inflammatory Molecules in Acute Myeloblastic Leukemia |
| title_short | Elastase II Gene Encoding as Inflammatory Molecules in Acute Myeloblastic Leukemia |
| title_sort | elastase ii gene encoding as inflammatory molecules in acute myeloblastic leukemia |
| title_unstemmed | Elastase II Gene Encoding as Inflammatory Molecules in Acute Myeloblastic Leukemia |
| topic | General Medicine, Immunology, Immunology and Allergy |
| url | http://dx.doi.org/10.1177/1721727x0800600204 |