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Radiosensitization and Anti-tumour Effects of Cytosine Deaminase and Thymidine Kinase Fusion Suicide Gene in Human Adenoid Cystic Carcinoma Cells
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| Zeitschriftentitel: | Journal of International Medical Research |
|---|---|
| Personen und Körperschaften: | , , , , , , |
| In: | Journal of International Medical Research, 37, 2009, 2, S. 479-490 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
SAGE Publications
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| Schlagwörter: |
| author_facet |
Huang, S-Y Zhang, D-S Han, J-Q Zhang, N Zhang, S-Z Mu, W-L Wei, F-C Huang, S-Y Zhang, D-S Han, J-Q Zhang, N Zhang, S-Z Mu, W-L Wei, F-C |
|---|---|
| author |
Huang, S-Y Zhang, D-S Han, J-Q Zhang, N Zhang, S-Z Mu, W-L Wei, F-C |
| spellingShingle |
Huang, S-Y Zhang, D-S Han, J-Q Zhang, N Zhang, S-Z Mu, W-L Wei, F-C Journal of International Medical Research Radiosensitization and Anti-tumour Effects of Cytosine Deaminase and Thymidine Kinase Fusion Suicide Gene in Human Adenoid Cystic Carcinoma Cells Biochemistry (medical) Cell Biology Biochemistry General Medicine |
| author_sort |
huang, s-y |
| spelling |
Huang, S-Y Zhang, D-S Han, J-Q Zhang, N Zhang, S-Z Mu, W-L Wei, F-C 0300-0605 1473-2300 SAGE Publications Biochemistry (medical) Cell Biology Biochemistry General Medicine http://dx.doi.org/10.1177/147323000903700224 <jats:p> Herpes simplex virus thymidine kinase (HSV-TK) and Escherichia coli cytosine deaminase (CD) can convert innocuous prodrugs into cytotoxic metabolites and are being investigated for use in gene therapy for cancer. Human adenoid cystic carcinoma (ACC-2) cells transduced with a CD/HSV-TK fusion gene (ACC-2/CD-TK cells) were found to be more sensitive to radiation than ACC-2 cells when exposed to 5-fluorocytosine (5-FC; 40 μg/ml) plus ganciclovir (0.1 μg/ml) for 48 h before irradiation. Analysis of radiation survival curves for cells exposed to 5-FC plus ganciclovir before irradiation showed that ACC-2 cells had a higher capacity for sublethal damage repair (D<jats:sub>q</jats:sub> value) and greater cellular radiosensitivity (D<jats:sub>0</jats:sub> value) than ACC-2/CD-TK cells. Colony formation rate after 2 Gy of irradiation was significantly greater for ACC-2 than for ACC-2/CD-TK cells when cells were treated with 5-FC plus ganciclovir before irradiation. This study, therefore, indicates that addition of radiation might substantially improve the therapeutic potential of CD-TK fusion gene therapy of human adenoid cystic carcinomas. </jats:p> Radiosensitization and Anti-tumour Effects of Cytosine Deaminase and Thymidine Kinase Fusion Suicide Gene in Human Adenoid Cystic Carcinoma Cells Journal of International Medical Research |
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SAGE Publications, 2009 |
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SAGE Publications, 2009 |
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| title |
Radiosensitization and Anti-tumour Effects of Cytosine Deaminase and Thymidine Kinase Fusion Suicide Gene in Human Adenoid Cystic Carcinoma Cells |
| title_unstemmed |
Radiosensitization and Anti-tumour Effects of Cytosine Deaminase and Thymidine Kinase Fusion Suicide Gene in Human Adenoid Cystic Carcinoma Cells |
| title_full |
Radiosensitization and Anti-tumour Effects of Cytosine Deaminase and Thymidine Kinase Fusion Suicide Gene in Human Adenoid Cystic Carcinoma Cells |
| title_fullStr |
Radiosensitization and Anti-tumour Effects of Cytosine Deaminase and Thymidine Kinase Fusion Suicide Gene in Human Adenoid Cystic Carcinoma Cells |
| title_full_unstemmed |
Radiosensitization and Anti-tumour Effects of Cytosine Deaminase and Thymidine Kinase Fusion Suicide Gene in Human Adenoid Cystic Carcinoma Cells |
| title_short |
Radiosensitization and Anti-tumour Effects of Cytosine Deaminase and Thymidine Kinase Fusion Suicide Gene in Human Adenoid Cystic Carcinoma Cells |
| title_sort |
radiosensitization and anti-tumour effects of cytosine deaminase and thymidine kinase fusion suicide gene in human adenoid cystic carcinoma cells |
| topic |
Biochemistry (medical) Cell Biology Biochemistry General Medicine |
| url |
http://dx.doi.org/10.1177/147323000903700224 |
| publishDate |
2009 |
| physical |
479-490 |
| description |
<jats:p> Herpes simplex virus thymidine kinase (HSV-TK) and Escherichia coli cytosine deaminase (CD) can convert innocuous prodrugs into cytotoxic metabolites and are being investigated for use in gene therapy for cancer. Human adenoid cystic carcinoma (ACC-2) cells transduced with a CD/HSV-TK fusion gene (ACC-2/CD-TK cells) were found to be more sensitive to radiation than ACC-2 cells when exposed to 5-fluorocytosine (5-FC; 40 μg/ml) plus ganciclovir (0.1 μg/ml) for 48 h before irradiation. Analysis of radiation survival curves for cells exposed to 5-FC plus ganciclovir before irradiation showed that ACC-2 cells had a higher capacity for sublethal damage repair (D<jats:sub>q</jats:sub> value) and greater cellular radiosensitivity (D<jats:sub>0</jats:sub> value) than ACC-2/CD-TK cells. Colony formation rate after 2 Gy of irradiation was significantly greater for ACC-2 than for ACC-2/CD-TK cells when cells were treated with 5-FC plus ganciclovir before irradiation. This study, therefore, indicates that addition of radiation might substantially improve the therapeutic potential of CD-TK fusion gene therapy of human adenoid cystic carcinomas. </jats:p> |
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| author | Huang, S-Y, Zhang, D-S, Han, J-Q, Zhang, N, Zhang, S-Z, Mu, W-L, Wei, F-C |
| author_facet | Huang, S-Y, Zhang, D-S, Han, J-Q, Zhang, N, Zhang, S-Z, Mu, W-L, Wei, F-C, Huang, S-Y, Zhang, D-S, Han, J-Q, Zhang, N, Zhang, S-Z, Mu, W-L, Wei, F-C |
| author_sort | huang, s-y |
| container_issue | 2 |
| container_start_page | 479 |
| container_title | Journal of International Medical Research |
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| description | <jats:p> Herpes simplex virus thymidine kinase (HSV-TK) and Escherichia coli cytosine deaminase (CD) can convert innocuous prodrugs into cytotoxic metabolites and are being investigated for use in gene therapy for cancer. Human adenoid cystic carcinoma (ACC-2) cells transduced with a CD/HSV-TK fusion gene (ACC-2/CD-TK cells) were found to be more sensitive to radiation than ACC-2 cells when exposed to 5-fluorocytosine (5-FC; 40 μg/ml) plus ganciclovir (0.1 μg/ml) for 48 h before irradiation. Analysis of radiation survival curves for cells exposed to 5-FC plus ganciclovir before irradiation showed that ACC-2 cells had a higher capacity for sublethal damage repair (D<jats:sub>q</jats:sub> value) and greater cellular radiosensitivity (D<jats:sub>0</jats:sub> value) than ACC-2/CD-TK cells. Colony formation rate after 2 Gy of irradiation was significantly greater for ACC-2 than for ACC-2/CD-TK cells when cells were treated with 5-FC plus ganciclovir before irradiation. This study, therefore, indicates that addition of radiation might substantially improve the therapeutic potential of CD-TK fusion gene therapy of human adenoid cystic carcinomas. </jats:p> |
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| imprint | SAGE Publications, 2009 |
| imprint_str_mv | SAGE Publications, 2009 |
| institution | DE-Bn3, DE-Brt1, DE-D161, DE-Zwi2, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275 |
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| physical | 479-490 |
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| spelling | Huang, S-Y Zhang, D-S Han, J-Q Zhang, N Zhang, S-Z Mu, W-L Wei, F-C 0300-0605 1473-2300 SAGE Publications Biochemistry (medical) Cell Biology Biochemistry General Medicine http://dx.doi.org/10.1177/147323000903700224 <jats:p> Herpes simplex virus thymidine kinase (HSV-TK) and Escherichia coli cytosine deaminase (CD) can convert innocuous prodrugs into cytotoxic metabolites and are being investigated for use in gene therapy for cancer. Human adenoid cystic carcinoma (ACC-2) cells transduced with a CD/HSV-TK fusion gene (ACC-2/CD-TK cells) were found to be more sensitive to radiation than ACC-2 cells when exposed to 5-fluorocytosine (5-FC; 40 μg/ml) plus ganciclovir (0.1 μg/ml) for 48 h before irradiation. Analysis of radiation survival curves for cells exposed to 5-FC plus ganciclovir before irradiation showed that ACC-2 cells had a higher capacity for sublethal damage repair (D<jats:sub>q</jats:sub> value) and greater cellular radiosensitivity (D<jats:sub>0</jats:sub> value) than ACC-2/CD-TK cells. Colony formation rate after 2 Gy of irradiation was significantly greater for ACC-2 than for ACC-2/CD-TK cells when cells were treated with 5-FC plus ganciclovir before irradiation. This study, therefore, indicates that addition of radiation might substantially improve the therapeutic potential of CD-TK fusion gene therapy of human adenoid cystic carcinomas. </jats:p> Radiosensitization and Anti-tumour Effects of Cytosine Deaminase and Thymidine Kinase Fusion Suicide Gene in Human Adenoid Cystic Carcinoma Cells Journal of International Medical Research |
| spellingShingle | Huang, S-Y, Zhang, D-S, Han, J-Q, Zhang, N, Zhang, S-Z, Mu, W-L, Wei, F-C, Journal of International Medical Research, Radiosensitization and Anti-tumour Effects of Cytosine Deaminase and Thymidine Kinase Fusion Suicide Gene in Human Adenoid Cystic Carcinoma Cells, Biochemistry (medical), Cell Biology, Biochemistry, General Medicine |
| title | Radiosensitization and Anti-tumour Effects of Cytosine Deaminase and Thymidine Kinase Fusion Suicide Gene in Human Adenoid Cystic Carcinoma Cells |
| title_full | Radiosensitization and Anti-tumour Effects of Cytosine Deaminase and Thymidine Kinase Fusion Suicide Gene in Human Adenoid Cystic Carcinoma Cells |
| title_fullStr | Radiosensitization and Anti-tumour Effects of Cytosine Deaminase and Thymidine Kinase Fusion Suicide Gene in Human Adenoid Cystic Carcinoma Cells |
| title_full_unstemmed | Radiosensitization and Anti-tumour Effects of Cytosine Deaminase and Thymidine Kinase Fusion Suicide Gene in Human Adenoid Cystic Carcinoma Cells |
| title_short | Radiosensitization and Anti-tumour Effects of Cytosine Deaminase and Thymidine Kinase Fusion Suicide Gene in Human Adenoid Cystic Carcinoma Cells |
| title_sort | radiosensitization and anti-tumour effects of cytosine deaminase and thymidine kinase fusion suicide gene in human adenoid cystic carcinoma cells |
| title_unstemmed | Radiosensitization and Anti-tumour Effects of Cytosine Deaminase and Thymidine Kinase Fusion Suicide Gene in Human Adenoid Cystic Carcinoma Cells |
| topic | Biochemistry (medical), Cell Biology, Biochemistry, General Medicine |
| url | http://dx.doi.org/10.1177/147323000903700224 |