Eintrag weiter verarbeiten
Abstract A38: Maternal embryonic leucine zipper kinase (MELK) is upregulated in high-grade prostate cancer
Gespeichert in:
| Zeitschriftentitel: | Cancer Research |
|---|---|
| Personen und Körperschaften: | , , , , , , , , , |
| In: | Cancer Research, 72, 2012, 4_Supplement, S. A38-A38 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Association for Cancer Research (AACR)
|
| Schlagwörter: |
| author_facet |
Kuner, Ruprecht Sültmann, Holger Fälth, Maria Pressinotti, Nicole Chui Brase, Jan Christoph Balaguer-Puig, Sabrina Schäfer, Georg Bartsch, Georg Steiner, Eberhard Klocker, Helmut Kuner, Ruprecht Sültmann, Holger Fälth, Maria Pressinotti, Nicole Chui Brase, Jan Christoph Balaguer-Puig, Sabrina Schäfer, Georg Bartsch, Georg Steiner, Eberhard Klocker, Helmut |
|---|---|
| author |
Kuner, Ruprecht Sültmann, Holger Fälth, Maria Pressinotti, Nicole Chui Brase, Jan Christoph Balaguer-Puig, Sabrina Schäfer, Georg Bartsch, Georg Steiner, Eberhard Klocker, Helmut |
| spellingShingle |
Kuner, Ruprecht Sültmann, Holger Fälth, Maria Pressinotti, Nicole Chui Brase, Jan Christoph Balaguer-Puig, Sabrina Schäfer, Georg Bartsch, Georg Steiner, Eberhard Klocker, Helmut Cancer Research Abstract A38: Maternal embryonic leucine zipper kinase (MELK) is upregulated in high-grade prostate cancer Cancer Research Oncology |
| author_sort |
kuner, ruprecht |
| spelling |
Kuner, Ruprecht Sültmann, Holger Fälth, Maria Pressinotti, Nicole Chui Brase, Jan Christoph Balaguer-Puig, Sabrina Schäfer, Georg Bartsch, Georg Steiner, Eberhard Klocker, Helmut 0008-5472 1538-7445 American Association for Cancer Research (AACR) Cancer Research Oncology http://dx.doi.org/10.1158/1538-7445.prca2012-a38 <jats:title>Abstract</jats:title> <jats:p>Loss of cell cycle control is a prerequisite for cancer onset and progression. In prostate cancer, increased activity of cell cycle proliferating genes has been associated with prognostic parameters such as biochemical relapse and survival. The identification of novel oncogenic and druggable targets in patient subgroups with poor prognosis may help to develop targeted therapy approaches.</jats:p> <jats:p>We analyzed prostate cancer and corresponding benign tissues (n = 98) using microarrays. The comparison of high and low grade tumors (Gleason score ≥ 4+3 vs. ≤ 3+4) revealed 144 differentially expressed genes (p &lt; 0.05), which are associated with a severe disease course. Out of these, 15 genes are assigned to cell cycle process. The gene maternal embryonic leucine zipper kinase (MELK) was identified to be highly correlated with cell cycle and prostate cancer associated genes like UBE2C, TOP2A, CCNB2 and AURKB, and was therefore further included into validation and functional experiments. The association between MELK gene expression and high-risk prostate cancer was validated in an independent patient cohort (p &lt; 0.005, n = 79). Furthermore, our finding was supported by a public microarray dataset showing a higher MELK expression in prostate cancer patients with biochemical relapse. Immunohistochemical analysis using a tissue microarray (n = 94) revealed increased MELK protein expression in prostate cancer tissues of high Gleason scores. Knockdown of MELK decreased cell viability in PC3 and LNCaP cells. Microarray analysis upon MELK knockdown experiments revealed downstream processes like chromatin modification, embryonic development and cell migration. Our findings indicate that the protein serine/threonine kinase MELK represents a promising marker for advanced prostate cancer disease.</jats:p> <jats:p>Citation Format: Ruprecht Kuner, Holger Sültmann, Maria Fälth, Nicole Chui Pressinotti, Jan Christoph Brase, Sabrina Balaguer-Puig, Georg Schäfer, Georg Bartsch, Eberhard Steiner, Helmut Klocker. Maternal embryonic leucine zipper kinase (MELK) is upregulated in highgrade prostate cancer [abstract]. In: Proceedings of the AACR Special Conference on Advances in Prostate Cancer Research; 2012 Feb 6-9; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2012;72(4 Suppl):Abstract nr A38.</jats:p> Abstract A38: Maternal embryonic leucine zipper kinase (MELK) is upregulated in high-grade prostate cancer Cancer Research |
| doi_str_mv |
10.1158/1538-7445.prca2012-a38 |
| facet_avail |
Online Free |
| finc_class_facet |
Medizin |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTE1OC8xNTM4LTc0NDUucHJjYTIwMTItYTM4 |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTE1OC8xNTM4LTc0NDUucHJjYTIwMTItYTM4 |
| institution |
DE-Ch1 DE-L229 DE-D275 DE-Bn3 DE-Brt1 DE-Zwi2 DE-D161 DE-Gla1 DE-Zi4 DE-15 DE-Pl11 DE-Rs1 DE-105 DE-14 |
| imprint |
American Association for Cancer Research (AACR), 2012 |
| imprint_str_mv |
American Association for Cancer Research (AACR), 2012 |
| issn |
1538-7445 0008-5472 |
| issn_str_mv |
1538-7445 0008-5472 |
| language |
English |
| mega_collection |
American Association for Cancer Research (AACR) (CrossRef) |
| match_str |
kuner2012abstracta38maternalembryonicleucinezipperkinasemelkisupregulatedinhighgradeprostatecancer |
| publishDateSort |
2012 |
| publisher |
American Association for Cancer Research (AACR) |
| recordtype |
ai |
| record_format |
ai |
| series |
Cancer Research |
| source_id |
49 |
| title |
Abstract A38: Maternal embryonic leucine zipper kinase (MELK) is upregulated in high-grade prostate cancer |
| title_unstemmed |
Abstract A38: Maternal embryonic leucine zipper kinase (MELK) is upregulated in high-grade prostate cancer |
| title_full |
Abstract A38: Maternal embryonic leucine zipper kinase (MELK) is upregulated in high-grade prostate cancer |
| title_fullStr |
Abstract A38: Maternal embryonic leucine zipper kinase (MELK) is upregulated in high-grade prostate cancer |
| title_full_unstemmed |
Abstract A38: Maternal embryonic leucine zipper kinase (MELK) is upregulated in high-grade prostate cancer |
| title_short |
Abstract A38: Maternal embryonic leucine zipper kinase (MELK) is upregulated in high-grade prostate cancer |
| title_sort |
abstract a38: maternal embryonic leucine zipper kinase (melk) is upregulated in high-grade prostate cancer |
| topic |
Cancer Research Oncology |
| url |
http://dx.doi.org/10.1158/1538-7445.prca2012-a38 |
| publishDate |
2012 |
| physical |
A38-A38 |
| description |
<jats:title>Abstract</jats:title>
<jats:p>Loss of cell cycle control is a prerequisite for cancer onset and progression. In prostate cancer, increased activity of cell cycle proliferating genes has been associated with prognostic parameters such as biochemical relapse and survival. The identification of novel oncogenic and druggable targets in patient subgroups with poor prognosis may help to develop targeted therapy approaches.</jats:p>
<jats:p>We analyzed prostate cancer and corresponding benign tissues (n = 98) using microarrays. The comparison of high and low grade tumors (Gleason score ≥ 4+3 vs. ≤ 3+4) revealed 144 differentially expressed genes (p &lt; 0.05), which are associated with a severe disease course. Out of these, 15 genes are assigned to cell cycle process. The gene maternal embryonic leucine zipper kinase (MELK) was identified to be highly correlated with cell cycle and prostate cancer associated genes like UBE2C, TOP2A, CCNB2 and AURKB, and was therefore further included into validation and functional experiments. The association between MELK gene expression and high-risk prostate cancer was validated in an independent patient cohort (p &lt; 0.005, n = 79). Furthermore, our finding was supported by a public microarray dataset showing a higher MELK expression in prostate cancer patients with biochemical relapse. Immunohistochemical analysis using a tissue microarray (n = 94) revealed increased MELK protein expression in prostate cancer tissues of high Gleason scores. Knockdown of MELK decreased cell viability in PC3 and LNCaP cells. Microarray analysis upon MELK knockdown experiments revealed downstream processes like chromatin modification, embryonic development and cell migration. Our findings indicate that the protein serine/threonine kinase MELK represents a promising marker for advanced prostate cancer disease.</jats:p>
<jats:p>Citation Format: Ruprecht Kuner, Holger Sültmann, Maria Fälth, Nicole Chui Pressinotti, Jan Christoph Brase, Sabrina Balaguer-Puig, Georg Schäfer, Georg Bartsch, Eberhard Steiner, Helmut Klocker. Maternal embryonic leucine zipper kinase (MELK) is upregulated in highgrade prostate cancer [abstract]. In: Proceedings of the AACR Special Conference on Advances in Prostate Cancer Research; 2012 Feb 6-9; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2012;72(4 Suppl):Abstract nr A38.</jats:p> |
| container_issue |
4_Supplement |
| container_start_page |
0 |
| container_title |
Cancer Research |
| container_volume |
72 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792328276300005379 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T12:50:00.058Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Abstract+A38%3A+Maternal+embryonic+leucine+zipper+kinase+%28MELK%29+is+upregulated+in+high-grade+prostate+cancer&rft.date=2012-02-06&genre=article&issn=1538-7445&volume=72&issue=4_Supplement&pages=A38-A38&jtitle=Cancer+Research&atitle=Abstract+A38%3A+Maternal+embryonic+leucine+zipper+kinase+%28MELK%29+is+upregulated+in+high-grade+prostate+cancer&aulast=Klocker&aufirst=Helmut&rft_id=info%3Adoi%2F10.1158%2F1538-7445.prca2012-a38&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792328276300005379 |
| author | Kuner, Ruprecht, Sültmann, Holger, Fälth, Maria, Pressinotti, Nicole Chui, Brase, Jan Christoph, Balaguer-Puig, Sabrina, Schäfer, Georg, Bartsch, Georg, Steiner, Eberhard, Klocker, Helmut |
| author_facet | Kuner, Ruprecht, Sültmann, Holger, Fälth, Maria, Pressinotti, Nicole Chui, Brase, Jan Christoph, Balaguer-Puig, Sabrina, Schäfer, Georg, Bartsch, Georg, Steiner, Eberhard, Klocker, Helmut, Kuner, Ruprecht, Sültmann, Holger, Fälth, Maria, Pressinotti, Nicole Chui, Brase, Jan Christoph, Balaguer-Puig, Sabrina, Schäfer, Georg, Bartsch, Georg, Steiner, Eberhard, Klocker, Helmut |
| author_sort | kuner, ruprecht |
| container_issue | 4_Supplement |
| container_start_page | 0 |
| container_title | Cancer Research |
| container_volume | 72 |
| description | <jats:title>Abstract</jats:title> <jats:p>Loss of cell cycle control is a prerequisite for cancer onset and progression. In prostate cancer, increased activity of cell cycle proliferating genes has been associated with prognostic parameters such as biochemical relapse and survival. The identification of novel oncogenic and druggable targets in patient subgroups with poor prognosis may help to develop targeted therapy approaches.</jats:p> <jats:p>We analyzed prostate cancer and corresponding benign tissues (n = 98) using microarrays. The comparison of high and low grade tumors (Gleason score ≥ 4+3 vs. ≤ 3+4) revealed 144 differentially expressed genes (p &lt; 0.05), which are associated with a severe disease course. Out of these, 15 genes are assigned to cell cycle process. The gene maternal embryonic leucine zipper kinase (MELK) was identified to be highly correlated with cell cycle and prostate cancer associated genes like UBE2C, TOP2A, CCNB2 and AURKB, and was therefore further included into validation and functional experiments. The association between MELK gene expression and high-risk prostate cancer was validated in an independent patient cohort (p &lt; 0.005, n = 79). Furthermore, our finding was supported by a public microarray dataset showing a higher MELK expression in prostate cancer patients with biochemical relapse. Immunohistochemical analysis using a tissue microarray (n = 94) revealed increased MELK protein expression in prostate cancer tissues of high Gleason scores. Knockdown of MELK decreased cell viability in PC3 and LNCaP cells. Microarray analysis upon MELK knockdown experiments revealed downstream processes like chromatin modification, embryonic development and cell migration. Our findings indicate that the protein serine/threonine kinase MELK represents a promising marker for advanced prostate cancer disease.</jats:p> <jats:p>Citation Format: Ruprecht Kuner, Holger Sültmann, Maria Fälth, Nicole Chui Pressinotti, Jan Christoph Brase, Sabrina Balaguer-Puig, Georg Schäfer, Georg Bartsch, Eberhard Steiner, Helmut Klocker. Maternal embryonic leucine zipper kinase (MELK) is upregulated in highgrade prostate cancer [abstract]. In: Proceedings of the AACR Special Conference on Advances in Prostate Cancer Research; 2012 Feb 6-9; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2012;72(4 Suppl):Abstract nr A38.</jats:p> |
| doi_str_mv | 10.1158/1538-7445.prca2012-a38 |
| facet_avail | Online, Free |
| finc_class_facet | Medizin |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTE1OC8xNTM4LTc0NDUucHJjYTIwMTItYTM4 |
| imprint | American Association for Cancer Research (AACR), 2012 |
| imprint_str_mv | American Association for Cancer Research (AACR), 2012 |
| institution | DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14 |
| issn | 1538-7445, 0008-5472 |
| issn_str_mv | 1538-7445, 0008-5472 |
| language | English |
| last_indexed | 2024-03-01T12:50:00.058Z |
| match_str | kuner2012abstracta38maternalembryonicleucinezipperkinasemelkisupregulatedinhighgradeprostatecancer |
| mega_collection | American Association for Cancer Research (AACR) (CrossRef) |
| physical | A38-A38 |
| publishDate | 2012 |
| publishDateSort | 2012 |
| publisher | American Association for Cancer Research (AACR) |
| record_format | ai |
| recordtype | ai |
| series | Cancer Research |
| source_id | 49 |
| spelling | Kuner, Ruprecht Sültmann, Holger Fälth, Maria Pressinotti, Nicole Chui Brase, Jan Christoph Balaguer-Puig, Sabrina Schäfer, Georg Bartsch, Georg Steiner, Eberhard Klocker, Helmut 0008-5472 1538-7445 American Association for Cancer Research (AACR) Cancer Research Oncology http://dx.doi.org/10.1158/1538-7445.prca2012-a38 <jats:title>Abstract</jats:title> <jats:p>Loss of cell cycle control is a prerequisite for cancer onset and progression. In prostate cancer, increased activity of cell cycle proliferating genes has been associated with prognostic parameters such as biochemical relapse and survival. The identification of novel oncogenic and druggable targets in patient subgroups with poor prognosis may help to develop targeted therapy approaches.</jats:p> <jats:p>We analyzed prostate cancer and corresponding benign tissues (n = 98) using microarrays. The comparison of high and low grade tumors (Gleason score ≥ 4+3 vs. ≤ 3+4) revealed 144 differentially expressed genes (p &lt; 0.05), which are associated with a severe disease course. Out of these, 15 genes are assigned to cell cycle process. The gene maternal embryonic leucine zipper kinase (MELK) was identified to be highly correlated with cell cycle and prostate cancer associated genes like UBE2C, TOP2A, CCNB2 and AURKB, and was therefore further included into validation and functional experiments. The association between MELK gene expression and high-risk prostate cancer was validated in an independent patient cohort (p &lt; 0.005, n = 79). Furthermore, our finding was supported by a public microarray dataset showing a higher MELK expression in prostate cancer patients with biochemical relapse. Immunohistochemical analysis using a tissue microarray (n = 94) revealed increased MELK protein expression in prostate cancer tissues of high Gleason scores. Knockdown of MELK decreased cell viability in PC3 and LNCaP cells. Microarray analysis upon MELK knockdown experiments revealed downstream processes like chromatin modification, embryonic development and cell migration. Our findings indicate that the protein serine/threonine kinase MELK represents a promising marker for advanced prostate cancer disease.</jats:p> <jats:p>Citation Format: Ruprecht Kuner, Holger Sültmann, Maria Fälth, Nicole Chui Pressinotti, Jan Christoph Brase, Sabrina Balaguer-Puig, Georg Schäfer, Georg Bartsch, Eberhard Steiner, Helmut Klocker. Maternal embryonic leucine zipper kinase (MELK) is upregulated in highgrade prostate cancer [abstract]. In: Proceedings of the AACR Special Conference on Advances in Prostate Cancer Research; 2012 Feb 6-9; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2012;72(4 Suppl):Abstract nr A38.</jats:p> Abstract A38: Maternal embryonic leucine zipper kinase (MELK) is upregulated in high-grade prostate cancer Cancer Research |
| spellingShingle | Kuner, Ruprecht, Sültmann, Holger, Fälth, Maria, Pressinotti, Nicole Chui, Brase, Jan Christoph, Balaguer-Puig, Sabrina, Schäfer, Georg, Bartsch, Georg, Steiner, Eberhard, Klocker, Helmut, Cancer Research, Abstract A38: Maternal embryonic leucine zipper kinase (MELK) is upregulated in high-grade prostate cancer, Cancer Research, Oncology |
| title | Abstract A38: Maternal embryonic leucine zipper kinase (MELK) is upregulated in high-grade prostate cancer |
| title_full | Abstract A38: Maternal embryonic leucine zipper kinase (MELK) is upregulated in high-grade prostate cancer |
| title_fullStr | Abstract A38: Maternal embryonic leucine zipper kinase (MELK) is upregulated in high-grade prostate cancer |
| title_full_unstemmed | Abstract A38: Maternal embryonic leucine zipper kinase (MELK) is upregulated in high-grade prostate cancer |
| title_short | Abstract A38: Maternal embryonic leucine zipper kinase (MELK) is upregulated in high-grade prostate cancer |
| title_sort | abstract a38: maternal embryonic leucine zipper kinase (melk) is upregulated in high-grade prostate cancer |
| title_unstemmed | Abstract A38: Maternal embryonic leucine zipper kinase (MELK) is upregulated in high-grade prostate cancer |
| topic | Cancer Research, Oncology |
| url | http://dx.doi.org/10.1158/1538-7445.prca2012-a38 |