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Tumor β-1,4-Galactosyltransferase IV Overexpression Is Closely Associated with Colorectal Cancer Metastasis and Poor Prognosis

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Bibliographische Detailangaben
Zeitschriftentitel: Clinical Cancer Research
Personen und Körperschaften: Chen, Wei-Shone, Chang, Hong-Yi, Li, Chung-Pin, Liu, Jacqueline Ming, Huang, Tze-Sing
In: Clinical Cancer Research, 11, 2005, 24, S. 8615-8622
Format: E-Article
Sprache: Englisch
veröffentlicht:
American Association for Cancer Research (AACR)
Schlagwörter:
Cancer Research
Oncology
author_facet Chen, Wei-Shone
Chang, Hong-Yi
Li, Chung-Pin
Liu, Jacqueline Ming
Huang, Tze-Sing
Chen, Wei-Shone
Chang, Hong-Yi
Li, Chung-Pin
Liu, Jacqueline Ming
Huang, Tze-Sing
author Chen, Wei-Shone
Chang, Hong-Yi
Li, Chung-Pin
Liu, Jacqueline Ming
Huang, Tze-Sing
spellingShingle Chen, Wei-Shone
Chang, Hong-Yi
Li, Chung-Pin
Liu, Jacqueline Ming
Huang, Tze-Sing
Clinical Cancer Research
Tumor β-1,4-Galactosyltransferase IV Overexpression Is Closely Associated with Colorectal Cancer Metastasis and Poor Prognosis
Cancer Research
Oncology
author_sort chen, wei-shone
spelling Chen, Wei-Shone Chang, Hong-Yi Li, Chung-Pin Liu, Jacqueline Ming Huang, Tze-Sing 1078-0432 1557-3265 American Association for Cancer Research (AACR) Cancer Research Oncology http://dx.doi.org/10.1158/1078-0432.ccr-05-1006 <jats:title>Abstract</jats:title> <jats:p>Purpose: To elucidate the significance of β-1,4-galactosyltransferase IV (β-1,4-GT-IV) in the clinical presentation and prognostication of colorectal cancer.</jats:p> <jats:p>Experimental Design: Tissue lysates from paired tumor and nontumor tissues of a colon cancer patient were labeled separately with fluorescent dyes Cy5 and Cy3 for two-dimensional difference in-gel electrophoresis. Subsequent matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and immunoblot analyses identified a down-regulated level of β-1,4-GT-IV in the tumor tissue. In the follow-up study, paired tissue lysates were obtained from 100 colorectal cancer patients with immunoblot analyses done to compare the levels of β-1,4-GT-IV expression in these patients.</jats:p> <jats:p>Results: Of 100 colorectal patients studied, 48% had down-regulated expression of β-1,4-GT-IV in the tumor tissue but 28% of patients exhibited elevated β-1,4-GT-IV levels. Increased β-1,4-GT-IV in the tumor tissue was significantly coexistent with raised serum level of CA-199 and the presence of tumor metastasis (P = 0.006 and P &amp;lt; 0.001, respectively) but was independent of age and gender of patient, tumor site, tumor size, serum level of carcinoembryonic antigen, grade of tumor cell differentiation, and depth of tumor invasion. The results of logistic regression analyses suggested that tumor β-1,4-GT-IV overexpression and tumor invasion, but not other patient variables such as tumor size and serum levels of carcinoembryonic antigen and CA19-9, were significantly correlated with the occurrence of metastases (P &amp;lt; 0.05). In a multivariate regression analysis, the patient group with tumor β-1,4-GT-IV overexpression strongly predicted for tumor metastasis (odds ratio, 10.009; 95% confidence interval, 2.992-33.484; P &amp;lt; 0.001). Likewise, tumor β-1,4-GT-IV overexpression was significantly associated with poor overall survival (P &amp;lt; 0.01). By Cox regression analysis, this association remained significant even after adjustment for tumor metastasis (P = 0.048).</jats:p> <jats:p>Conclusion: Increased β-1,4-GT-IV expression in tumor tissue was strongly associated with tumor metastases and poor prognosis in colorectal cancer.</jats:p> Tumor β-1,4-Galactosyltransferase IV Overexpression Is Closely Associated with Colorectal Cancer Metastasis and Poor Prognosis Clinical Cancer Research
doi_str_mv 10.1158/1078-0432.ccr-05-1006
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title Tumor β-1,4-Galactosyltransferase IV Overexpression Is Closely Associated with Colorectal Cancer Metastasis and Poor Prognosis
title_unstemmed Tumor β-1,4-Galactosyltransferase IV Overexpression Is Closely Associated with Colorectal Cancer Metastasis and Poor Prognosis
title_full Tumor β-1,4-Galactosyltransferase IV Overexpression Is Closely Associated with Colorectal Cancer Metastasis and Poor Prognosis
title_fullStr Tumor β-1,4-Galactosyltransferase IV Overexpression Is Closely Associated with Colorectal Cancer Metastasis and Poor Prognosis
title_full_unstemmed Tumor β-1,4-Galactosyltransferase IV Overexpression Is Closely Associated with Colorectal Cancer Metastasis and Poor Prognosis
title_short Tumor β-1,4-Galactosyltransferase IV Overexpression Is Closely Associated with Colorectal Cancer Metastasis and Poor Prognosis
title_sort tumor β-1,4-galactosyltransferase iv overexpression is closely associated with colorectal cancer metastasis and poor prognosis
topic Cancer Research
Oncology
url http://dx.doi.org/10.1158/1078-0432.ccr-05-1006
publishDate 2005
physical 8615-8622
description <jats:title>Abstract</jats:title> <jats:p>Purpose: To elucidate the significance of β-1,4-galactosyltransferase IV (β-1,4-GT-IV) in the clinical presentation and prognostication of colorectal cancer.</jats:p> <jats:p>Experimental Design: Tissue lysates from paired tumor and nontumor tissues of a colon cancer patient were labeled separately with fluorescent dyes Cy5 and Cy3 for two-dimensional difference in-gel electrophoresis. Subsequent matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and immunoblot analyses identified a down-regulated level of β-1,4-GT-IV in the tumor tissue. In the follow-up study, paired tissue lysates were obtained from 100 colorectal cancer patients with immunoblot analyses done to compare the levels of β-1,4-GT-IV expression in these patients.</jats:p> <jats:p>Results: Of 100 colorectal patients studied, 48% had down-regulated expression of β-1,4-GT-IV in the tumor tissue but 28% of patients exhibited elevated β-1,4-GT-IV levels. Increased β-1,4-GT-IV in the tumor tissue was significantly coexistent with raised serum level of CA-199 and the presence of tumor metastasis (P = 0.006 and P &amp;lt; 0.001, respectively) but was independent of age and gender of patient, tumor site, tumor size, serum level of carcinoembryonic antigen, grade of tumor cell differentiation, and depth of tumor invasion. The results of logistic regression analyses suggested that tumor β-1,4-GT-IV overexpression and tumor invasion, but not other patient variables such as tumor size and serum levels of carcinoembryonic antigen and CA19-9, were significantly correlated with the occurrence of metastases (P &amp;lt; 0.05). In a multivariate regression analysis, the patient group with tumor β-1,4-GT-IV overexpression strongly predicted for tumor metastasis (odds ratio, 10.009; 95% confidence interval, 2.992-33.484; P &amp;lt; 0.001). Likewise, tumor β-1,4-GT-IV overexpression was significantly associated with poor overall survival (P &amp;lt; 0.01). By Cox regression analysis, this association remained significant even after adjustment for tumor metastasis (P = 0.048).</jats:p> <jats:p>Conclusion: Increased β-1,4-GT-IV expression in tumor tissue was strongly associated with tumor metastases and poor prognosis in colorectal cancer.</jats:p>
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author Chen, Wei-Shone, Chang, Hong-Yi, Li, Chung-Pin, Liu, Jacqueline Ming, Huang, Tze-Sing
author_facet Chen, Wei-Shone, Chang, Hong-Yi, Li, Chung-Pin, Liu, Jacqueline Ming, Huang, Tze-Sing, Chen, Wei-Shone, Chang, Hong-Yi, Li, Chung-Pin, Liu, Jacqueline Ming, Huang, Tze-Sing
author_sort chen, wei-shone
container_issue 24
container_start_page 8615
container_title Clinical Cancer Research
container_volume 11
description <jats:title>Abstract</jats:title> <jats:p>Purpose: To elucidate the significance of β-1,4-galactosyltransferase IV (β-1,4-GT-IV) in the clinical presentation and prognostication of colorectal cancer.</jats:p> <jats:p>Experimental Design: Tissue lysates from paired tumor and nontumor tissues of a colon cancer patient were labeled separately with fluorescent dyes Cy5 and Cy3 for two-dimensional difference in-gel electrophoresis. Subsequent matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and immunoblot analyses identified a down-regulated level of β-1,4-GT-IV in the tumor tissue. In the follow-up study, paired tissue lysates were obtained from 100 colorectal cancer patients with immunoblot analyses done to compare the levels of β-1,4-GT-IV expression in these patients.</jats:p> <jats:p>Results: Of 100 colorectal patients studied, 48% had down-regulated expression of β-1,4-GT-IV in the tumor tissue but 28% of patients exhibited elevated β-1,4-GT-IV levels. Increased β-1,4-GT-IV in the tumor tissue was significantly coexistent with raised serum level of CA-199 and the presence of tumor metastasis (P = 0.006 and P &amp;lt; 0.001, respectively) but was independent of age and gender of patient, tumor site, tumor size, serum level of carcinoembryonic antigen, grade of tumor cell differentiation, and depth of tumor invasion. The results of logistic regression analyses suggested that tumor β-1,4-GT-IV overexpression and tumor invasion, but not other patient variables such as tumor size and serum levels of carcinoembryonic antigen and CA19-9, were significantly correlated with the occurrence of metastases (P &amp;lt; 0.05). In a multivariate regression analysis, the patient group with tumor β-1,4-GT-IV overexpression strongly predicted for tumor metastasis (odds ratio, 10.009; 95% confidence interval, 2.992-33.484; P &amp;lt; 0.001). Likewise, tumor β-1,4-GT-IV overexpression was significantly associated with poor overall survival (P &amp;lt; 0.01). By Cox regression analysis, this association remained significant even after adjustment for tumor metastasis (P = 0.048).</jats:p> <jats:p>Conclusion: Increased β-1,4-GT-IV expression in tumor tissue was strongly associated with tumor metastases and poor prognosis in colorectal cancer.</jats:p>
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spelling Chen, Wei-Shone Chang, Hong-Yi Li, Chung-Pin Liu, Jacqueline Ming Huang, Tze-Sing 1078-0432 1557-3265 American Association for Cancer Research (AACR) Cancer Research Oncology http://dx.doi.org/10.1158/1078-0432.ccr-05-1006 <jats:title>Abstract</jats:title> <jats:p>Purpose: To elucidate the significance of β-1,4-galactosyltransferase IV (β-1,4-GT-IV) in the clinical presentation and prognostication of colorectal cancer.</jats:p> <jats:p>Experimental Design: Tissue lysates from paired tumor and nontumor tissues of a colon cancer patient were labeled separately with fluorescent dyes Cy5 and Cy3 for two-dimensional difference in-gel electrophoresis. Subsequent matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and immunoblot analyses identified a down-regulated level of β-1,4-GT-IV in the tumor tissue. In the follow-up study, paired tissue lysates were obtained from 100 colorectal cancer patients with immunoblot analyses done to compare the levels of β-1,4-GT-IV expression in these patients.</jats:p> <jats:p>Results: Of 100 colorectal patients studied, 48% had down-regulated expression of β-1,4-GT-IV in the tumor tissue but 28% of patients exhibited elevated β-1,4-GT-IV levels. Increased β-1,4-GT-IV in the tumor tissue was significantly coexistent with raised serum level of CA-199 and the presence of tumor metastasis (P = 0.006 and P &amp;lt; 0.001, respectively) but was independent of age and gender of patient, tumor site, tumor size, serum level of carcinoembryonic antigen, grade of tumor cell differentiation, and depth of tumor invasion. The results of logistic regression analyses suggested that tumor β-1,4-GT-IV overexpression and tumor invasion, but not other patient variables such as tumor size and serum levels of carcinoembryonic antigen and CA19-9, were significantly correlated with the occurrence of metastases (P &amp;lt; 0.05). In a multivariate regression analysis, the patient group with tumor β-1,4-GT-IV overexpression strongly predicted for tumor metastasis (odds ratio, 10.009; 95% confidence interval, 2.992-33.484; P &amp;lt; 0.001). Likewise, tumor β-1,4-GT-IV overexpression was significantly associated with poor overall survival (P &amp;lt; 0.01). By Cox regression analysis, this association remained significant even after adjustment for tumor metastasis (P = 0.048).</jats:p> <jats:p>Conclusion: Increased β-1,4-GT-IV expression in tumor tissue was strongly associated with tumor metastases and poor prognosis in colorectal cancer.</jats:p> Tumor β-1,4-Galactosyltransferase IV Overexpression Is Closely Associated with Colorectal Cancer Metastasis and Poor Prognosis Clinical Cancer Research
spellingShingle Chen, Wei-Shone, Chang, Hong-Yi, Li, Chung-Pin, Liu, Jacqueline Ming, Huang, Tze-Sing, Clinical Cancer Research, Tumor β-1,4-Galactosyltransferase IV Overexpression Is Closely Associated with Colorectal Cancer Metastasis and Poor Prognosis, Cancer Research, Oncology
title Tumor β-1,4-Galactosyltransferase IV Overexpression Is Closely Associated with Colorectal Cancer Metastasis and Poor Prognosis
title_full Tumor β-1,4-Galactosyltransferase IV Overexpression Is Closely Associated with Colorectal Cancer Metastasis and Poor Prognosis
title_fullStr Tumor β-1,4-Galactosyltransferase IV Overexpression Is Closely Associated with Colorectal Cancer Metastasis and Poor Prognosis
title_full_unstemmed Tumor β-1,4-Galactosyltransferase IV Overexpression Is Closely Associated with Colorectal Cancer Metastasis and Poor Prognosis
title_short Tumor β-1,4-Galactosyltransferase IV Overexpression Is Closely Associated with Colorectal Cancer Metastasis and Poor Prognosis
title_sort tumor β-1,4-galactosyltransferase iv overexpression is closely associated with colorectal cancer metastasis and poor prognosis
title_unstemmed Tumor β-1,4-Galactosyltransferase IV Overexpression Is Closely Associated with Colorectal Cancer Metastasis and Poor Prognosis
topic Cancer Research, Oncology
url http://dx.doi.org/10.1158/1078-0432.ccr-05-1006