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Cellular Localization and Biochemical Characterization of a Chimeric Fluorescent Protein Fusion ofArabidopsisCellulose Synthase-Like A2 Inserted into Golgi Membrane
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| Zeitschriftentitel: | The Scientific World Journal |
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| Personen und Körperschaften: | , , , , , , |
| In: | The Scientific World Journal, 2014, 2014, S. 1-7 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Hindawi Limited
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| Schlagwörter: |
| author_facet |
De Caroli, Monica Lenucci, Marcello S. Di Sansebastiano, Gian-Pietro Tunno, Michela Montefusco, Anna Dalessandro, Giuseppe Piro, Gabriella De Caroli, Monica Lenucci, Marcello S. Di Sansebastiano, Gian-Pietro Tunno, Michela Montefusco, Anna Dalessandro, Giuseppe Piro, Gabriella |
|---|---|
| author |
De Caroli, Monica Lenucci, Marcello S. Di Sansebastiano, Gian-Pietro Tunno, Michela Montefusco, Anna Dalessandro, Giuseppe Piro, Gabriella |
| spellingShingle |
De Caroli, Monica Lenucci, Marcello S. Di Sansebastiano, Gian-Pietro Tunno, Michela Montefusco, Anna Dalessandro, Giuseppe Piro, Gabriella The Scientific World Journal Cellular Localization and Biochemical Characterization of a Chimeric Fluorescent Protein Fusion ofArabidopsisCellulose Synthase-Like A2 Inserted into Golgi Membrane General Environmental Science General Biochemistry, Genetics and Molecular Biology General Medicine |
| author_sort |
de caroli, monica |
| spelling |
De Caroli, Monica Lenucci, Marcello S. Di Sansebastiano, Gian-Pietro Tunno, Michela Montefusco, Anna Dalessandro, Giuseppe Piro, Gabriella 2356-6140 1537-744X Hindawi Limited General Environmental Science General Biochemistry, Genetics and Molecular Biology General Medicine http://dx.doi.org/10.1155/2014/792420 <jats:p><jats:italic>Cellulose synthase</jats:italic>-<jats:italic>like</jats:italic>(<jats:italic>Csl</jats:italic>) genes are believed to encode enzymes for the synthesis of cell wall matrix polysaccharides. The subfamily of<jats:italic>CslA</jats:italic>is putatively involved in the biosynthesis of<jats:italic>β</jats:italic>-mannans. Here we report a study on the cellular localization and the enzyme activity of an<jats:italic>Arabidopsis</jats:italic>CslA family member, AtCslA2. We show that the fluorescent protein fusion AtCslA2-GFP, transiently expressed in tobacco leaf protoplasts, is synthesized in the ER and it accumulates in the Golgi stacks. The chimera is inserted in the Golgi membrane and is functional since membrane preparations obtained by transformed protoplasts carry out the<jats:italic>in vitro</jats:italic>synthesis of a<jats:sup>14</jats:sup>C-mannan starting from GDP-<jats:sc>d</jats:sc>-[U-<jats:sup>14</jats:sup>C]mannose as substrate. The enzyme specific activity is increased by approximately 38% in the transformed protoplasts with respect to wild-type. Preliminary tests with proteinase K, biochemical data, and TM domain predictions suggest that the catalytic site of AtCslA2 faces the Golgi lumen.</jats:p> Cellular Localization and Biochemical Characterization of a Chimeric Fluorescent Protein Fusion of<i>Arabidopsis</i>Cellulose Synthase-Like A2 Inserted into Golgi Membrane The Scientific World Journal |
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| title |
Cellular Localization and Biochemical Characterization of a Chimeric Fluorescent Protein Fusion ofArabidopsisCellulose Synthase-Like A2 Inserted into Golgi Membrane |
| title_unstemmed |
Cellular Localization and Biochemical Characterization of a Chimeric Fluorescent Protein Fusion ofArabidopsisCellulose Synthase-Like A2 Inserted into Golgi Membrane |
| title_full |
Cellular Localization and Biochemical Characterization of a Chimeric Fluorescent Protein Fusion ofArabidopsisCellulose Synthase-Like A2 Inserted into Golgi Membrane |
| title_fullStr |
Cellular Localization and Biochemical Characterization of a Chimeric Fluorescent Protein Fusion ofArabidopsisCellulose Synthase-Like A2 Inserted into Golgi Membrane |
| title_full_unstemmed |
Cellular Localization and Biochemical Characterization of a Chimeric Fluorescent Protein Fusion ofArabidopsisCellulose Synthase-Like A2 Inserted into Golgi Membrane |
| title_short |
Cellular Localization and Biochemical Characterization of a Chimeric Fluorescent Protein Fusion ofArabidopsisCellulose Synthase-Like A2 Inserted into Golgi Membrane |
| title_sort |
cellular localization and biochemical characterization of a chimeric fluorescent protein fusion of<i>arabidopsis</i>cellulose synthase-like a2 inserted into golgi membrane |
| topic |
General Environmental Science General Biochemistry, Genetics and Molecular Biology General Medicine |
| url |
http://dx.doi.org/10.1155/2014/792420 |
| publishDate |
2014 |
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1-7 |
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<jats:p><jats:italic>Cellulose synthase</jats:italic>-<jats:italic>like</jats:italic>(<jats:italic>Csl</jats:italic>) genes are believed to encode enzymes for the synthesis of cell wall matrix polysaccharides. The subfamily of<jats:italic>CslA</jats:italic>is putatively involved in the biosynthesis of<jats:italic>β</jats:italic>-mannans. Here we report a study on the cellular localization and the enzyme activity of an<jats:italic>Arabidopsis</jats:italic>CslA family member, AtCslA2. We show that the fluorescent protein fusion AtCslA2-GFP, transiently expressed in tobacco leaf protoplasts, is synthesized in the ER and it accumulates in the Golgi stacks. The chimera is inserted in the Golgi membrane and is functional since membrane preparations obtained by transformed protoplasts carry out the<jats:italic>in vitro</jats:italic>synthesis of a<jats:sup>14</jats:sup>C-mannan starting from GDP-<jats:sc>d</jats:sc>-[U-<jats:sup>14</jats:sup>C]mannose as substrate. The enzyme specific activity is increased by approximately 38% in the transformed protoplasts with respect to wild-type. Preliminary tests with proteinase K, biochemical data, and TM domain predictions suggest that the catalytic site of AtCslA2 faces the Golgi lumen.</jats:p> |
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| author | De Caroli, Monica, Lenucci, Marcello S., Di Sansebastiano, Gian-Pietro, Tunno, Michela, Montefusco, Anna, Dalessandro, Giuseppe, Piro, Gabriella |
| author_facet | De Caroli, Monica, Lenucci, Marcello S., Di Sansebastiano, Gian-Pietro, Tunno, Michela, Montefusco, Anna, Dalessandro, Giuseppe, Piro, Gabriella, De Caroli, Monica, Lenucci, Marcello S., Di Sansebastiano, Gian-Pietro, Tunno, Michela, Montefusco, Anna, Dalessandro, Giuseppe, Piro, Gabriella |
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| description | <jats:p><jats:italic>Cellulose synthase</jats:italic>-<jats:italic>like</jats:italic>(<jats:italic>Csl</jats:italic>) genes are believed to encode enzymes for the synthesis of cell wall matrix polysaccharides. The subfamily of<jats:italic>CslA</jats:italic>is putatively involved in the biosynthesis of<jats:italic>β</jats:italic>-mannans. Here we report a study on the cellular localization and the enzyme activity of an<jats:italic>Arabidopsis</jats:italic>CslA family member, AtCslA2. We show that the fluorescent protein fusion AtCslA2-GFP, transiently expressed in tobacco leaf protoplasts, is synthesized in the ER and it accumulates in the Golgi stacks. The chimera is inserted in the Golgi membrane and is functional since membrane preparations obtained by transformed protoplasts carry out the<jats:italic>in vitro</jats:italic>synthesis of a<jats:sup>14</jats:sup>C-mannan starting from GDP-<jats:sc>d</jats:sc>-[U-<jats:sup>14</jats:sup>C]mannose as substrate. The enzyme specific activity is increased by approximately 38% in the transformed protoplasts with respect to wild-type. Preliminary tests with proteinase K, biochemical data, and TM domain predictions suggest that the catalytic site of AtCslA2 faces the Golgi lumen.</jats:p> |
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| spelling | De Caroli, Monica Lenucci, Marcello S. Di Sansebastiano, Gian-Pietro Tunno, Michela Montefusco, Anna Dalessandro, Giuseppe Piro, Gabriella 2356-6140 1537-744X Hindawi Limited General Environmental Science General Biochemistry, Genetics and Molecular Biology General Medicine http://dx.doi.org/10.1155/2014/792420 <jats:p><jats:italic>Cellulose synthase</jats:italic>-<jats:italic>like</jats:italic>(<jats:italic>Csl</jats:italic>) genes are believed to encode enzymes for the synthesis of cell wall matrix polysaccharides. The subfamily of<jats:italic>CslA</jats:italic>is putatively involved in the biosynthesis of<jats:italic>β</jats:italic>-mannans. Here we report a study on the cellular localization and the enzyme activity of an<jats:italic>Arabidopsis</jats:italic>CslA family member, AtCslA2. We show that the fluorescent protein fusion AtCslA2-GFP, transiently expressed in tobacco leaf protoplasts, is synthesized in the ER and it accumulates in the Golgi stacks. The chimera is inserted in the Golgi membrane and is functional since membrane preparations obtained by transformed protoplasts carry out the<jats:italic>in vitro</jats:italic>synthesis of a<jats:sup>14</jats:sup>C-mannan starting from GDP-<jats:sc>d</jats:sc>-[U-<jats:sup>14</jats:sup>C]mannose as substrate. The enzyme specific activity is increased by approximately 38% in the transformed protoplasts with respect to wild-type. Preliminary tests with proteinase K, biochemical data, and TM domain predictions suggest that the catalytic site of AtCslA2 faces the Golgi lumen.</jats:p> Cellular Localization and Biochemical Characterization of a Chimeric Fluorescent Protein Fusion of<i>Arabidopsis</i>Cellulose Synthase-Like A2 Inserted into Golgi Membrane The Scientific World Journal |
| spellingShingle | De Caroli, Monica, Lenucci, Marcello S., Di Sansebastiano, Gian-Pietro, Tunno, Michela, Montefusco, Anna, Dalessandro, Giuseppe, Piro, Gabriella, The Scientific World Journal, Cellular Localization and Biochemical Characterization of a Chimeric Fluorescent Protein Fusion ofArabidopsisCellulose Synthase-Like A2 Inserted into Golgi Membrane, General Environmental Science, General Biochemistry, Genetics and Molecular Biology, General Medicine |
| title | Cellular Localization and Biochemical Characterization of a Chimeric Fluorescent Protein Fusion ofArabidopsisCellulose Synthase-Like A2 Inserted into Golgi Membrane |
| title_full | Cellular Localization and Biochemical Characterization of a Chimeric Fluorescent Protein Fusion ofArabidopsisCellulose Synthase-Like A2 Inserted into Golgi Membrane |
| title_fullStr | Cellular Localization and Biochemical Characterization of a Chimeric Fluorescent Protein Fusion ofArabidopsisCellulose Synthase-Like A2 Inserted into Golgi Membrane |
| title_full_unstemmed | Cellular Localization and Biochemical Characterization of a Chimeric Fluorescent Protein Fusion ofArabidopsisCellulose Synthase-Like A2 Inserted into Golgi Membrane |
| title_short | Cellular Localization and Biochemical Characterization of a Chimeric Fluorescent Protein Fusion ofArabidopsisCellulose Synthase-Like A2 Inserted into Golgi Membrane |
| title_sort | cellular localization and biochemical characterization of a chimeric fluorescent protein fusion of<i>arabidopsis</i>cellulose synthase-like a2 inserted into golgi membrane |
| title_unstemmed | Cellular Localization and Biochemical Characterization of a Chimeric Fluorescent Protein Fusion ofArabidopsisCellulose Synthase-Like A2 Inserted into Golgi Membrane |
| topic | General Environmental Science, General Biochemistry, Genetics and Molecular Biology, General Medicine |
| url | http://dx.doi.org/10.1155/2014/792420 |