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Functional Roles of Kv1 Channels in Neocortical Pyramidal Neurons
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| Zeitschriftentitel: | Journal of Neurophysiology |
|---|---|
| Personen und Körperschaften: | , , , , |
| In: | Journal of Neurophysiology, 97, 2007, 3, S. 1931-1940 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Physiological Society
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| Schlagwörter: |
| author_facet |
Guan, D. Lee, J. C. F. Higgs, M. H. Spain, W. J. Foehring, R. C. Guan, D. Lee, J. C. F. Higgs, M. H. Spain, W. J. Foehring, R. C. |
|---|---|
| author |
Guan, D. Lee, J. C. F. Higgs, M. H. Spain, W. J. Foehring, R. C. |
| spellingShingle |
Guan, D. Lee, J. C. F. Higgs, M. H. Spain, W. J. Foehring, R. C. Journal of Neurophysiology Functional Roles of Kv1 Channels in Neocortical Pyramidal Neurons Physiology General Neuroscience |
| author_sort |
guan, d. |
| spelling |
Guan, D. Lee, J. C. F. Higgs, M. H. Spain, W. J. Foehring, R. C. 0022-3077 1522-1598 American Physiological Society Physiology General Neuroscience http://dx.doi.org/10.1152/jn.00933.2006 <jats:p> Pyramidal neurons from layers II/III of somatosensory and motor cortex express multiple Kv1 α-subunits and a current sensitive to block by α-dendrotoxin (α-DTX). We examined functional roles of native Kv1 channels in these cells using current-clamp recordings in brain slices and current- and voltage-clamp recordings in dissociated cells. α-DTX caused a significant negative shift in voltage threshold for action potentials (APs) and reduced rheobase. Correspondingly, a ramp-voltage protocol revealed that the α-DTX–sensitive current activated at subthreshold voltages. AP width at threshold increased with successive APs during repetitive firing. The steady-state threshold width for a given firing rate was similar in control and α-DTX, despite an initially broader AP in α-DTX. AP voltage threshold increased similarly during a train of spikes under control conditions and in the presence of α-DTX. α-DTX had no effect on input resistance or resting membrane potential and modest effects on the amplitude or width of a single AP. Accordingly, experiments using AP waveforms (APWs) as voltage protocols revealed that α-DTX–sensitive current peaked late during the AP repolarization phase. Application of α-DTX increased the rate of firing to intracellular current injection and increased gain (multiplicative effects), but did not alter spike-frequency adaptation. Consistent with these findings, voltage-clamp experiments revealed that the proportion of outward current sensitive to α-DTX was highest during the interval between two APWs, reflecting slow deactivation kinetics at −50 mV. Finally, α-DTX did not alter the selectivity of pyramidal neurons for DC versus time-varying stimuli. </jats:p> Functional Roles of Kv1 Channels in Neocortical Pyramidal Neurons Journal of Neurophysiology |
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| title |
Functional Roles of Kv1 Channels in Neocortical Pyramidal Neurons |
| title_unstemmed |
Functional Roles of Kv1 Channels in Neocortical Pyramidal Neurons |
| title_full |
Functional Roles of Kv1 Channels in Neocortical Pyramidal Neurons |
| title_fullStr |
Functional Roles of Kv1 Channels in Neocortical Pyramidal Neurons |
| title_full_unstemmed |
Functional Roles of Kv1 Channels in Neocortical Pyramidal Neurons |
| title_short |
Functional Roles of Kv1 Channels in Neocortical Pyramidal Neurons |
| title_sort |
functional roles of kv1 channels in neocortical pyramidal neurons |
| topic |
Physiology General Neuroscience |
| url |
http://dx.doi.org/10.1152/jn.00933.2006 |
| publishDate |
2007 |
| physical |
1931-1940 |
| description |
<jats:p> Pyramidal neurons from layers II/III of somatosensory and motor cortex express multiple Kv1 α-subunits and a current sensitive to block by α-dendrotoxin (α-DTX). We examined functional roles of native Kv1 channels in these cells using current-clamp recordings in brain slices and current- and voltage-clamp recordings in dissociated cells. α-DTX caused a significant negative shift in voltage threshold for action potentials (APs) and reduced rheobase. Correspondingly, a ramp-voltage protocol revealed that the α-DTX–sensitive current activated at subthreshold voltages. AP width at threshold increased with successive APs during repetitive firing. The steady-state threshold width for a given firing rate was similar in control and α-DTX, despite an initially broader AP in α-DTX. AP voltage threshold increased similarly during a train of spikes under control conditions and in the presence of α-DTX. α-DTX had no effect on input resistance or resting membrane potential and modest effects on the amplitude or width of a single AP. Accordingly, experiments using AP waveforms (APWs) as voltage protocols revealed that α-DTX–sensitive current peaked late during the AP repolarization phase. Application of α-DTX increased the rate of firing to intracellular current injection and increased gain (multiplicative effects), but did not alter spike-frequency adaptation. Consistent with these findings, voltage-clamp experiments revealed that the proportion of outward current sensitive to α-DTX was highest during the interval between two APWs, reflecting slow deactivation kinetics at −50 mV. Finally, α-DTX did not alter the selectivity of pyramidal neurons for DC versus time-varying stimuli. </jats:p> |
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| author | Guan, D., Lee, J. C. F., Higgs, M. H., Spain, W. J., Foehring, R. C. |
| author_facet | Guan, D., Lee, J. C. F., Higgs, M. H., Spain, W. J., Foehring, R. C., Guan, D., Lee, J. C. F., Higgs, M. H., Spain, W. J., Foehring, R. C. |
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| container_title | Journal of Neurophysiology |
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| description | <jats:p> Pyramidal neurons from layers II/III of somatosensory and motor cortex express multiple Kv1 α-subunits and a current sensitive to block by α-dendrotoxin (α-DTX). We examined functional roles of native Kv1 channels in these cells using current-clamp recordings in brain slices and current- and voltage-clamp recordings in dissociated cells. α-DTX caused a significant negative shift in voltage threshold for action potentials (APs) and reduced rheobase. Correspondingly, a ramp-voltage protocol revealed that the α-DTX–sensitive current activated at subthreshold voltages. AP width at threshold increased with successive APs during repetitive firing. The steady-state threshold width for a given firing rate was similar in control and α-DTX, despite an initially broader AP in α-DTX. AP voltage threshold increased similarly during a train of spikes under control conditions and in the presence of α-DTX. α-DTX had no effect on input resistance or resting membrane potential and modest effects on the amplitude or width of a single AP. Accordingly, experiments using AP waveforms (APWs) as voltage protocols revealed that α-DTX–sensitive current peaked late during the AP repolarization phase. Application of α-DTX increased the rate of firing to intracellular current injection and increased gain (multiplicative effects), but did not alter spike-frequency adaptation. Consistent with these findings, voltage-clamp experiments revealed that the proportion of outward current sensitive to α-DTX was highest during the interval between two APWs, reflecting slow deactivation kinetics at −50 mV. Finally, α-DTX did not alter the selectivity of pyramidal neurons for DC versus time-varying stimuli. </jats:p> |
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| spelling | Guan, D. Lee, J. C. F. Higgs, M. H. Spain, W. J. Foehring, R. C. 0022-3077 1522-1598 American Physiological Society Physiology General Neuroscience http://dx.doi.org/10.1152/jn.00933.2006 <jats:p> Pyramidal neurons from layers II/III of somatosensory and motor cortex express multiple Kv1 α-subunits and a current sensitive to block by α-dendrotoxin (α-DTX). We examined functional roles of native Kv1 channels in these cells using current-clamp recordings in brain slices and current- and voltage-clamp recordings in dissociated cells. α-DTX caused a significant negative shift in voltage threshold for action potentials (APs) and reduced rheobase. Correspondingly, a ramp-voltage protocol revealed that the α-DTX–sensitive current activated at subthreshold voltages. AP width at threshold increased with successive APs during repetitive firing. The steady-state threshold width for a given firing rate was similar in control and α-DTX, despite an initially broader AP in α-DTX. AP voltage threshold increased similarly during a train of spikes under control conditions and in the presence of α-DTX. α-DTX had no effect on input resistance or resting membrane potential and modest effects on the amplitude or width of a single AP. Accordingly, experiments using AP waveforms (APWs) as voltage protocols revealed that α-DTX–sensitive current peaked late during the AP repolarization phase. Application of α-DTX increased the rate of firing to intracellular current injection and increased gain (multiplicative effects), but did not alter spike-frequency adaptation. Consistent with these findings, voltage-clamp experiments revealed that the proportion of outward current sensitive to α-DTX was highest during the interval between two APWs, reflecting slow deactivation kinetics at −50 mV. Finally, α-DTX did not alter the selectivity of pyramidal neurons for DC versus time-varying stimuli. </jats:p> Functional Roles of Kv1 Channels in Neocortical Pyramidal Neurons Journal of Neurophysiology |
| spellingShingle | Guan, D., Lee, J. C. F., Higgs, M. H., Spain, W. J., Foehring, R. C., Journal of Neurophysiology, Functional Roles of Kv1 Channels in Neocortical Pyramidal Neurons, Physiology, General Neuroscience |
| title | Functional Roles of Kv1 Channels in Neocortical Pyramidal Neurons |
| title_full | Functional Roles of Kv1 Channels in Neocortical Pyramidal Neurons |
| title_fullStr | Functional Roles of Kv1 Channels in Neocortical Pyramidal Neurons |
| title_full_unstemmed | Functional Roles of Kv1 Channels in Neocortical Pyramidal Neurons |
| title_short | Functional Roles of Kv1 Channels in Neocortical Pyramidal Neurons |
| title_sort | functional roles of kv1 channels in neocortical pyramidal neurons |
| title_unstemmed | Functional Roles of Kv1 Channels in Neocortical Pyramidal Neurons |
| topic | Physiology, General Neuroscience |
| url | http://dx.doi.org/10.1152/jn.00933.2006 |