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Pyruvate induces mitochondrial biogenesis by a PGC-1 α-independent mechanism
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| Zeitschriftentitel: | American Journal of Physiology-Cell Physiology |
|---|---|
| Personen und Körperschaften: | , , , , |
| In: | American Journal of Physiology-Cell Physiology, 292, 2007, 5, S. C1599-C1605 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Physiological Society
|
| Schlagwörter: |
| author_facet |
Wilson, Leanne Yang, Qing Szustakowski, Joseph D. Gullicksen, P. Scott Halse, Reza Wilson, Leanne Yang, Qing Szustakowski, Joseph D. Gullicksen, P. Scott Halse, Reza |
|---|---|
| author |
Wilson, Leanne Yang, Qing Szustakowski, Joseph D. Gullicksen, P. Scott Halse, Reza |
| spellingShingle |
Wilson, Leanne Yang, Qing Szustakowski, Joseph D. Gullicksen, P. Scott Halse, Reza American Journal of Physiology-Cell Physiology Pyruvate induces mitochondrial biogenesis by a PGC-1 α-independent mechanism Cell Biology Physiology |
| author_sort |
wilson, leanne |
| spelling |
Wilson, Leanne Yang, Qing Szustakowski, Joseph D. Gullicksen, P. Scott Halse, Reza 0363-6143 1522-1563 American Physiological Society Cell Biology Physiology http://dx.doi.org/10.1152/ajpcell.00428.2006 <jats:p>Oxidative cells increase mitochondrial mass in response to stimuli such as changes in energy demand or cellular differentiation. This plasticity enables the cell to adapt dynamically to achieve the necessary oxidative capacity. However, the pathways involved in triggering mitochondrial biogenesis are poorly defined. The present study examines the impact of altering energy provision on mitochondrial biogenesis in muscle cells. C2C12 myoblasts were chronically treated with supraphysiological levels of sodium pyruvate for 72 h. Treated cells exhibited increased mitochondrial protein expression, basal respiratory rate, and maximal oxidative capacity. The increase in mitochondrial biogenesis was independent of increases in peroxisomal proliferator activator receptor-γ coactivator-1α (PGC-1α) and PGC-1β mRNA expression. To further assess whether PGC-1α expression was necessary for pyruvate action, cells were infected with adenovirus containing shRNA for PGC-1α before treatment with pyruvate. Despite a 70% reduction in PGC-1α mRNA, the effect of pyruvate was preserved. Furthermore, pyruvate induced mitochondrial biogenesis in primary myoblasts from PGC-1α null mice. These data suggest that regulation of mitochondrial biogenesis by pyruvate in myoblasts is independent of PGC-1α, suggesting the existence of a novel energy-sensing pathway regulating oxidative capacity.</jats:p> Pyruvate induces mitochondrial biogenesis by a PGC-1 α-independent mechanism American Journal of Physiology-Cell Physiology |
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| title |
Pyruvate induces mitochondrial biogenesis by a PGC-1 α-independent mechanism |
| title_unstemmed |
Pyruvate induces mitochondrial biogenesis by a PGC-1 α-independent mechanism |
| title_full |
Pyruvate induces mitochondrial biogenesis by a PGC-1 α-independent mechanism |
| title_fullStr |
Pyruvate induces mitochondrial biogenesis by a PGC-1 α-independent mechanism |
| title_full_unstemmed |
Pyruvate induces mitochondrial biogenesis by a PGC-1 α-independent mechanism |
| title_short |
Pyruvate induces mitochondrial biogenesis by a PGC-1 α-independent mechanism |
| title_sort |
pyruvate induces mitochondrial biogenesis by a pgc-1 α-independent mechanism |
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Cell Biology Physiology |
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http://dx.doi.org/10.1152/ajpcell.00428.2006 |
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2007 |
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C1599-C1605 |
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<jats:p>Oxidative cells increase mitochondrial mass in response to stimuli such as changes in energy demand or cellular differentiation. This plasticity enables the cell to adapt dynamically to achieve the necessary oxidative capacity. However, the pathways involved in triggering mitochondrial biogenesis are poorly defined. The present study examines the impact of altering energy provision on mitochondrial biogenesis in muscle cells. C2C12 myoblasts were chronically treated with supraphysiological levels of sodium pyruvate for 72 h. Treated cells exhibited increased mitochondrial protein expression, basal respiratory rate, and maximal oxidative capacity. The increase in mitochondrial biogenesis was independent of increases in peroxisomal proliferator activator receptor-γ coactivator-1α (PGC-1α) and PGC-1β mRNA expression. To further assess whether PGC-1α expression was necessary for pyruvate action, cells were infected with adenovirus containing shRNA for PGC-1α before treatment with pyruvate. Despite a 70% reduction in PGC-1α mRNA, the effect of pyruvate was preserved. Furthermore, pyruvate induced mitochondrial biogenesis in primary myoblasts from PGC-1α null mice. These data suggest that regulation of mitochondrial biogenesis by pyruvate in myoblasts is independent of PGC-1α, suggesting the existence of a novel energy-sensing pathway regulating oxidative capacity.</jats:p> |
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| author | Wilson, Leanne, Yang, Qing, Szustakowski, Joseph D., Gullicksen, P. Scott, Halse, Reza |
| author_facet | Wilson, Leanne, Yang, Qing, Szustakowski, Joseph D., Gullicksen, P. Scott, Halse, Reza, Wilson, Leanne, Yang, Qing, Szustakowski, Joseph D., Gullicksen, P. Scott, Halse, Reza |
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| description | <jats:p>Oxidative cells increase mitochondrial mass in response to stimuli such as changes in energy demand or cellular differentiation. This plasticity enables the cell to adapt dynamically to achieve the necessary oxidative capacity. However, the pathways involved in triggering mitochondrial biogenesis are poorly defined. The present study examines the impact of altering energy provision on mitochondrial biogenesis in muscle cells. C2C12 myoblasts were chronically treated with supraphysiological levels of sodium pyruvate for 72 h. Treated cells exhibited increased mitochondrial protein expression, basal respiratory rate, and maximal oxidative capacity. The increase in mitochondrial biogenesis was independent of increases in peroxisomal proliferator activator receptor-γ coactivator-1α (PGC-1α) and PGC-1β mRNA expression. To further assess whether PGC-1α expression was necessary for pyruvate action, cells were infected with adenovirus containing shRNA for PGC-1α before treatment with pyruvate. Despite a 70% reduction in PGC-1α mRNA, the effect of pyruvate was preserved. Furthermore, pyruvate induced mitochondrial biogenesis in primary myoblasts from PGC-1α null mice. These data suggest that regulation of mitochondrial biogenesis by pyruvate in myoblasts is independent of PGC-1α, suggesting the existence of a novel energy-sensing pathway regulating oxidative capacity.</jats:p> |
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| spelling | Wilson, Leanne Yang, Qing Szustakowski, Joseph D. Gullicksen, P. Scott Halse, Reza 0363-6143 1522-1563 American Physiological Society Cell Biology Physiology http://dx.doi.org/10.1152/ajpcell.00428.2006 <jats:p>Oxidative cells increase mitochondrial mass in response to stimuli such as changes in energy demand or cellular differentiation. This plasticity enables the cell to adapt dynamically to achieve the necessary oxidative capacity. However, the pathways involved in triggering mitochondrial biogenesis are poorly defined. The present study examines the impact of altering energy provision on mitochondrial biogenesis in muscle cells. C2C12 myoblasts were chronically treated with supraphysiological levels of sodium pyruvate for 72 h. Treated cells exhibited increased mitochondrial protein expression, basal respiratory rate, and maximal oxidative capacity. The increase in mitochondrial biogenesis was independent of increases in peroxisomal proliferator activator receptor-γ coactivator-1α (PGC-1α) and PGC-1β mRNA expression. To further assess whether PGC-1α expression was necessary for pyruvate action, cells were infected with adenovirus containing shRNA for PGC-1α before treatment with pyruvate. Despite a 70% reduction in PGC-1α mRNA, the effect of pyruvate was preserved. Furthermore, pyruvate induced mitochondrial biogenesis in primary myoblasts from PGC-1α null mice. These data suggest that regulation of mitochondrial biogenesis by pyruvate in myoblasts is independent of PGC-1α, suggesting the existence of a novel energy-sensing pathway regulating oxidative capacity.</jats:p> Pyruvate induces mitochondrial biogenesis by a PGC-1 α-independent mechanism American Journal of Physiology-Cell Physiology |
| spellingShingle | Wilson, Leanne, Yang, Qing, Szustakowski, Joseph D., Gullicksen, P. Scott, Halse, Reza, American Journal of Physiology-Cell Physiology, Pyruvate induces mitochondrial biogenesis by a PGC-1 α-independent mechanism, Cell Biology, Physiology |
| title | Pyruvate induces mitochondrial biogenesis by a PGC-1 α-independent mechanism |
| title_full | Pyruvate induces mitochondrial biogenesis by a PGC-1 α-independent mechanism |
| title_fullStr | Pyruvate induces mitochondrial biogenesis by a PGC-1 α-independent mechanism |
| title_full_unstemmed | Pyruvate induces mitochondrial biogenesis by a PGC-1 α-independent mechanism |
| title_short | Pyruvate induces mitochondrial biogenesis by a PGC-1 α-independent mechanism |
| title_sort | pyruvate induces mitochondrial biogenesis by a pgc-1 α-independent mechanism |
| title_unstemmed | Pyruvate induces mitochondrial biogenesis by a PGC-1 α-independent mechanism |
| topic | Cell Biology, Physiology |
| url | http://dx.doi.org/10.1152/ajpcell.00428.2006 |