Eintrag weiter verarbeiten
Induction of angiotensin-converting enzyme after miR-143/145 deletion is critical for impaired smooth muscle contractility
Gespeichert in:
Zeitschriftentitel: | American Journal of Physiology-Cell Physiology |
---|---|
Personen und Körperschaften: | , , , , , , , |
In: | American Journal of Physiology-Cell Physiology, 307, 2014, 12, S. C1093-C1101 |
Format: | E-Article |
Sprache: | Englisch |
veröffentlicht: |
American Physiological Society
|
Schlagwörter: |
author_facet |
Dahan, Diana Ekman, Mari Larsson-Callerfelt, Anna-Karin Turczyńska, Karolina Boettger, Thomas Braun, Thomas Swärd, Karl Albinsson, Sebastian Dahan, Diana Ekman, Mari Larsson-Callerfelt, Anna-Karin Turczyńska, Karolina Boettger, Thomas Braun, Thomas Swärd, Karl Albinsson, Sebastian |
---|---|
author |
Dahan, Diana Ekman, Mari Larsson-Callerfelt, Anna-Karin Turczyńska, Karolina Boettger, Thomas Braun, Thomas Swärd, Karl Albinsson, Sebastian |
spellingShingle |
Dahan, Diana Ekman, Mari Larsson-Callerfelt, Anna-Karin Turczyńska, Karolina Boettger, Thomas Braun, Thomas Swärd, Karl Albinsson, Sebastian American Journal of Physiology-Cell Physiology Induction of angiotensin-converting enzyme after miR-143/145 deletion is critical for impaired smooth muscle contractility Cell Biology Physiology |
author_sort |
dahan, diana |
spelling |
Dahan, Diana Ekman, Mari Larsson-Callerfelt, Anna-Karin Turczyńska, Karolina Boettger, Thomas Braun, Thomas Swärd, Karl Albinsson, Sebastian 0363-6143 1522-1563 American Physiological Society Cell Biology Physiology http://dx.doi.org/10.1152/ajpcell.00250.2014 <jats:p> MicroRNAs have emerged as regulators of smooth muscle cell phenotype with a role in smooth muscle-related disease. Studies have shown that miR-143 and miR-145 are the most highly expressed microRNAs in smooth muscle cells, controlling differentiation and function. The effect of miR-143/145 knockout has been established in the vasculature but not in smooth muscle from other organs. Using knockout mice we found that maximal contraction induced by either depolarization or phosphatase inhibition was reduced in vascular and airway smooth muscle but maintained in the urinary bladder. Furthermore, a reduction of media thickness and reduced expression of differentiation markers was seen in the aorta but not in the bladder. Supporting the view that phenotype switching depends on a tissue-specific target of miR-143/145, we found induction of angiotensin-converting enzyme in the aorta but not in the bladder where angiotensin-converting enzyme was expressed at a low level. Chronic treatment with angiotensin type-1 receptor antagonist restored contractility in miR-143/145-deficient aorta while leaving bladder contractility unaffected. This shows that tissue-specific targets are critical for the effects of miR-143/145 on smooth muscle differentiation and that angiotensin converting enzyme is one such target. </jats:p> Induction of angiotensin-converting enzyme after miR-143/145 deletion is critical for impaired smooth muscle contractility American Journal of Physiology-Cell Physiology |
doi_str_mv |
10.1152/ajpcell.00250.2014 |
facet_avail |
Online Free |
finc_class_facet |
Biologie |
format |
ElectronicArticle |
fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTE1Mi9hanBjZWxsLjAwMjUwLjIwMTQ |
id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTE1Mi9hanBjZWxsLjAwMjUwLjIwMTQ |
institution |
DE-Bn3 DE-Brt1 DE-Zwi2 DE-D161 DE-Gla1 DE-Zi4 DE-15 DE-Pl11 DE-Rs1 DE-105 DE-14 DE-Ch1 DE-L229 DE-D275 |
imprint |
American Physiological Society, 2014 |
imprint_str_mv |
American Physiological Society, 2014 |
issn |
0363-6143 1522-1563 |
issn_str_mv |
0363-6143 1522-1563 |
language |
English |
mega_collection |
American Physiological Society (CrossRef) |
match_str |
dahan2014inductionofangiotensinconvertingenzymeaftermir143145deletioniscriticalforimpairedsmoothmusclecontractility |
publishDateSort |
2014 |
publisher |
American Physiological Society |
recordtype |
ai |
record_format |
ai |
series |
American Journal of Physiology-Cell Physiology |
source_id |
49 |
title |
Induction of angiotensin-converting enzyme after miR-143/145 deletion is critical for impaired smooth muscle contractility |
title_unstemmed |
Induction of angiotensin-converting enzyme after miR-143/145 deletion is critical for impaired smooth muscle contractility |
title_full |
Induction of angiotensin-converting enzyme after miR-143/145 deletion is critical for impaired smooth muscle contractility |
title_fullStr |
Induction of angiotensin-converting enzyme after miR-143/145 deletion is critical for impaired smooth muscle contractility |
title_full_unstemmed |
Induction of angiotensin-converting enzyme after miR-143/145 deletion is critical for impaired smooth muscle contractility |
title_short |
Induction of angiotensin-converting enzyme after miR-143/145 deletion is critical for impaired smooth muscle contractility |
title_sort |
induction of angiotensin-converting enzyme after mir-143/145 deletion is critical for impaired smooth muscle contractility |
topic |
Cell Biology Physiology |
url |
http://dx.doi.org/10.1152/ajpcell.00250.2014 |
publishDate |
2014 |
physical |
C1093-C1101 |
description |
<jats:p> MicroRNAs have emerged as regulators of smooth muscle cell phenotype with a role in smooth muscle-related disease. Studies have shown that miR-143 and miR-145 are the most highly expressed microRNAs in smooth muscle cells, controlling differentiation and function. The effect of miR-143/145 knockout has been established in the vasculature but not in smooth muscle from other organs. Using knockout mice we found that maximal contraction induced by either depolarization or phosphatase inhibition was reduced in vascular and airway smooth muscle but maintained in the urinary bladder. Furthermore, a reduction of media thickness and reduced expression of differentiation markers was seen in the aorta but not in the bladder. Supporting the view that phenotype switching depends on a tissue-specific target of miR-143/145, we found induction of angiotensin-converting enzyme in the aorta but not in the bladder where angiotensin-converting enzyme was expressed at a low level. Chronic treatment with angiotensin type-1 receptor antagonist restored contractility in miR-143/145-deficient aorta while leaving bladder contractility unaffected. This shows that tissue-specific targets are critical for the effects of miR-143/145 on smooth muscle differentiation and that angiotensin converting enzyme is one such target. </jats:p> |
container_issue |
12 |
container_start_page |
0 |
container_title |
American Journal of Physiology-Cell Physiology |
container_volume |
307 |
format_de105 |
Article, E-Article |
format_de14 |
Article, E-Article |
format_de15 |
Article, E-Article |
format_de520 |
Article, E-Article |
format_de540 |
Article, E-Article |
format_dech1 |
Article, E-Article |
format_ded117 |
Article, E-Article |
format_degla1 |
E-Article |
format_del152 |
Buch |
format_del189 |
Article, E-Article |
format_dezi4 |
Article |
format_dezwi2 |
Article, E-Article |
format_finc |
Article, E-Article |
format_nrw |
Article, E-Article |
_version_ |
1792335025735204870 |
geogr_code |
not assigned |
last_indexed |
2024-03-01T14:37:59.523Z |
geogr_code_person |
not assigned |
openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Induction+of+angiotensin-converting+enzyme+after+miR-143%2F145+deletion+is+critical+for+impaired+smooth+muscle+contractility&rft.date=2014-12-15&genre=article&issn=1522-1563&volume=307&issue=12&pages=C1093-C1101&jtitle=American+Journal+of+Physiology-Cell+Physiology&atitle=Induction+of+angiotensin-converting+enzyme+after+miR-143%2F145+deletion+is+critical+for+impaired+smooth+muscle+contractility&aulast=Albinsson&aufirst=Sebastian&rft_id=info%3Adoi%2F10.1152%2Fajpcell.00250.2014&rft.language%5B0%5D=eng |
SOLR | |
_version_ | 1792335025735204870 |
author | Dahan, Diana, Ekman, Mari, Larsson-Callerfelt, Anna-Karin, Turczyńska, Karolina, Boettger, Thomas, Braun, Thomas, Swärd, Karl, Albinsson, Sebastian |
author_facet | Dahan, Diana, Ekman, Mari, Larsson-Callerfelt, Anna-Karin, Turczyńska, Karolina, Boettger, Thomas, Braun, Thomas, Swärd, Karl, Albinsson, Sebastian, Dahan, Diana, Ekman, Mari, Larsson-Callerfelt, Anna-Karin, Turczyńska, Karolina, Boettger, Thomas, Braun, Thomas, Swärd, Karl, Albinsson, Sebastian |
author_sort | dahan, diana |
container_issue | 12 |
container_start_page | 0 |
container_title | American Journal of Physiology-Cell Physiology |
container_volume | 307 |
description | <jats:p> MicroRNAs have emerged as regulators of smooth muscle cell phenotype with a role in smooth muscle-related disease. Studies have shown that miR-143 and miR-145 are the most highly expressed microRNAs in smooth muscle cells, controlling differentiation and function. The effect of miR-143/145 knockout has been established in the vasculature but not in smooth muscle from other organs. Using knockout mice we found that maximal contraction induced by either depolarization or phosphatase inhibition was reduced in vascular and airway smooth muscle but maintained in the urinary bladder. Furthermore, a reduction of media thickness and reduced expression of differentiation markers was seen in the aorta but not in the bladder. Supporting the view that phenotype switching depends on a tissue-specific target of miR-143/145, we found induction of angiotensin-converting enzyme in the aorta but not in the bladder where angiotensin-converting enzyme was expressed at a low level. Chronic treatment with angiotensin type-1 receptor antagonist restored contractility in miR-143/145-deficient aorta while leaving bladder contractility unaffected. This shows that tissue-specific targets are critical for the effects of miR-143/145 on smooth muscle differentiation and that angiotensin converting enzyme is one such target. </jats:p> |
doi_str_mv | 10.1152/ajpcell.00250.2014 |
facet_avail | Online, Free |
finc_class_facet | Biologie |
format | ElectronicArticle |
format_de105 | Article, E-Article |
format_de14 | Article, E-Article |
format_de15 | Article, E-Article |
format_de520 | Article, E-Article |
format_de540 | Article, E-Article |
format_dech1 | Article, E-Article |
format_ded117 | Article, E-Article |
format_degla1 | E-Article |
format_del152 | Buch |
format_del189 | Article, E-Article |
format_dezi4 | Article |
format_dezwi2 | Article, E-Article |
format_finc | Article, E-Article |
format_nrw | Article, E-Article |
geogr_code | not assigned |
geogr_code_person | not assigned |
id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTE1Mi9hanBjZWxsLjAwMjUwLjIwMTQ |
imprint | American Physiological Society, 2014 |
imprint_str_mv | American Physiological Society, 2014 |
institution | DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275 |
issn | 0363-6143, 1522-1563 |
issn_str_mv | 0363-6143, 1522-1563 |
language | English |
last_indexed | 2024-03-01T14:37:59.523Z |
match_str | dahan2014inductionofangiotensinconvertingenzymeaftermir143145deletioniscriticalforimpairedsmoothmusclecontractility |
mega_collection | American Physiological Society (CrossRef) |
physical | C1093-C1101 |
publishDate | 2014 |
publishDateSort | 2014 |
publisher | American Physiological Society |
record_format | ai |
recordtype | ai |
series | American Journal of Physiology-Cell Physiology |
source_id | 49 |
spelling | Dahan, Diana Ekman, Mari Larsson-Callerfelt, Anna-Karin Turczyńska, Karolina Boettger, Thomas Braun, Thomas Swärd, Karl Albinsson, Sebastian 0363-6143 1522-1563 American Physiological Society Cell Biology Physiology http://dx.doi.org/10.1152/ajpcell.00250.2014 <jats:p> MicroRNAs have emerged as regulators of smooth muscle cell phenotype with a role in smooth muscle-related disease. Studies have shown that miR-143 and miR-145 are the most highly expressed microRNAs in smooth muscle cells, controlling differentiation and function. The effect of miR-143/145 knockout has been established in the vasculature but not in smooth muscle from other organs. Using knockout mice we found that maximal contraction induced by either depolarization or phosphatase inhibition was reduced in vascular and airway smooth muscle but maintained in the urinary bladder. Furthermore, a reduction of media thickness and reduced expression of differentiation markers was seen in the aorta but not in the bladder. Supporting the view that phenotype switching depends on a tissue-specific target of miR-143/145, we found induction of angiotensin-converting enzyme in the aorta but not in the bladder where angiotensin-converting enzyme was expressed at a low level. Chronic treatment with angiotensin type-1 receptor antagonist restored contractility in miR-143/145-deficient aorta while leaving bladder contractility unaffected. This shows that tissue-specific targets are critical for the effects of miR-143/145 on smooth muscle differentiation and that angiotensin converting enzyme is one such target. </jats:p> Induction of angiotensin-converting enzyme after miR-143/145 deletion is critical for impaired smooth muscle contractility American Journal of Physiology-Cell Physiology |
spellingShingle | Dahan, Diana, Ekman, Mari, Larsson-Callerfelt, Anna-Karin, Turczyńska, Karolina, Boettger, Thomas, Braun, Thomas, Swärd, Karl, Albinsson, Sebastian, American Journal of Physiology-Cell Physiology, Induction of angiotensin-converting enzyme after miR-143/145 deletion is critical for impaired smooth muscle contractility, Cell Biology, Physiology |
title | Induction of angiotensin-converting enzyme after miR-143/145 deletion is critical for impaired smooth muscle contractility |
title_full | Induction of angiotensin-converting enzyme after miR-143/145 deletion is critical for impaired smooth muscle contractility |
title_fullStr | Induction of angiotensin-converting enzyme after miR-143/145 deletion is critical for impaired smooth muscle contractility |
title_full_unstemmed | Induction of angiotensin-converting enzyme after miR-143/145 deletion is critical for impaired smooth muscle contractility |
title_short | Induction of angiotensin-converting enzyme after miR-143/145 deletion is critical for impaired smooth muscle contractility |
title_sort | induction of angiotensin-converting enzyme after mir-143/145 deletion is critical for impaired smooth muscle contractility |
title_unstemmed | Induction of angiotensin-converting enzyme after miR-143/145 deletion is critical for impaired smooth muscle contractility |
topic | Cell Biology, Physiology |
url | http://dx.doi.org/10.1152/ajpcell.00250.2014 |