Eintrag weiter verarbeiten
Label-free nonlinear optical microscopy detects early markers for osteogenic differentiation of human stem cells
Gespeichert in:
| Zeitschriftentitel: | Scientific Reports |
|---|---|
| Personen und Körperschaften: | , , , , , , , , , |
| In: | Scientific Reports, 6, 2016, 1 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Springer Science and Business Media LLC
|
| Schlagwörter: |
| author_facet |
Hofemeier, Arne D. Hachmeister, Henning Pilger, Christian Schürmann, Matthias Greiner, Johannes F. W. Nolte, Lena Sudhoff, Holger Kaltschmidt, Christian Huser, Thomas Kaltschmidt, Barbara Hofemeier, Arne D. Hachmeister, Henning Pilger, Christian Schürmann, Matthias Greiner, Johannes F. W. Nolte, Lena Sudhoff, Holger Kaltschmidt, Christian Huser, Thomas Kaltschmidt, Barbara |
|---|---|
| author |
Hofemeier, Arne D. Hachmeister, Henning Pilger, Christian Schürmann, Matthias Greiner, Johannes F. W. Nolte, Lena Sudhoff, Holger Kaltschmidt, Christian Huser, Thomas Kaltschmidt, Barbara |
| spellingShingle |
Hofemeier, Arne D. Hachmeister, Henning Pilger, Christian Schürmann, Matthias Greiner, Johannes F. W. Nolte, Lena Sudhoff, Holger Kaltschmidt, Christian Huser, Thomas Kaltschmidt, Barbara Scientific Reports Label-free nonlinear optical microscopy detects early markers for osteogenic differentiation of human stem cells Multidisciplinary |
| author_sort |
hofemeier, arne d. |
| spelling |
Hofemeier, Arne D. Hachmeister, Henning Pilger, Christian Schürmann, Matthias Greiner, Johannes F. W. Nolte, Lena Sudhoff, Holger Kaltschmidt, Christian Huser, Thomas Kaltschmidt, Barbara 2045-2322 Springer Science and Business Media LLC Multidisciplinary http://dx.doi.org/10.1038/srep26716 <jats:title>Abstract</jats:title><jats:p>Tissue engineering by stem cell differentiation is a novel treatment option for bone regeneration. Most approaches for the detection of osteogenic differentiation are invasive or destructive and not compatible with live cell analysis. Here, non-destructive and label-free approaches of Raman spectroscopy, coherent anti-Stokes Raman scattering (CARS) and second harmonic generation (SHG) microscopy were used to detect and image osteogenic differentiation of human neural crest-derived inferior turbinate stem cells (ITSCs). Combined CARS and SHG microscopy was able to detect markers of osteogenesis within 14 days after osteogenic induction. This process increased during continued differentiation. Furthermore, Raman spectroscopy showed significant increases of the PO<jats:sub>4</jats:sub><jats:sup>3−</jats:sup> symmetric stretch vibrations at 959 cm<jats:sup>−1</jats:sup> assigned to calcium hydroxyapatite between days 14 and 21. Additionally, CARS microscopy was able to image calcium hydroxyapatite deposits within 14 days following osteogenic induction, which was confirmed by Alizarin Red-Staining and RT- PCR. Taken together, the multimodal label-free analysis methods Raman spectroscopy, CARS and SHG microscopy can monitor osteogenic differentiation of adult human stem cells into osteoblasts with high sensitivity and spatial resolution in three dimensions. Our findings suggest a great potential of these optical detection methods for clinical applications including <jats:italic>in vivo</jats:italic> observation of bone tissue–implant-interfaces or disease diagnosis.</jats:p> Label-free nonlinear optical microscopy detects early markers for osteogenic differentiation of human stem cells Scientific Reports |
| doi_str_mv |
10.1038/srep26716 |
| facet_avail |
Online Free |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTAzOC9zcmVwMjY3MTY |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTAzOC9zcmVwMjY3MTY |
| institution |
DE-Bn3 DE-Brt1 DE-Zwi2 DE-D161 DE-Zi4 DE-Gla1 DE-15 DE-Pl11 DE-Rs1 DE-14 DE-105 DE-Ch1 DE-L229 DE-D275 |
| imprint |
Springer Science and Business Media LLC, 2016 |
| imprint_str_mv |
Springer Science and Business Media LLC, 2016 |
| issn |
2045-2322 |
| issn_str_mv |
2045-2322 |
| language |
English |
| mega_collection |
Springer Science and Business Media LLC (CrossRef) |
| match_str |
hofemeier2016labelfreenonlinearopticalmicroscopydetectsearlymarkersforosteogenicdifferentiationofhumanstemcells |
| publishDateSort |
2016 |
| publisher |
Springer Science and Business Media LLC |
| recordtype |
ai |
| record_format |
ai |
| series |
Scientific Reports |
| source_id |
49 |
| title |
Label-free nonlinear optical microscopy detects early markers for osteogenic differentiation of human stem cells |
| title_unstemmed |
Label-free nonlinear optical microscopy detects early markers for osteogenic differentiation of human stem cells |
| title_full |
Label-free nonlinear optical microscopy detects early markers for osteogenic differentiation of human stem cells |
| title_fullStr |
Label-free nonlinear optical microscopy detects early markers for osteogenic differentiation of human stem cells |
| title_full_unstemmed |
Label-free nonlinear optical microscopy detects early markers for osteogenic differentiation of human stem cells |
| title_short |
Label-free nonlinear optical microscopy detects early markers for osteogenic differentiation of human stem cells |
| title_sort |
label-free nonlinear optical microscopy detects early markers for osteogenic differentiation of human stem cells |
| topic |
Multidisciplinary |
| url |
http://dx.doi.org/10.1038/srep26716 |
| publishDate |
2016 |
| physical |
|
| description |
<jats:title>Abstract</jats:title><jats:p>Tissue engineering by stem cell differentiation is a novel treatment option for bone regeneration. Most approaches for the detection of osteogenic differentiation are invasive or destructive and not compatible with live cell analysis. Here, non-destructive and label-free approaches of Raman spectroscopy, coherent anti-Stokes Raman scattering (CARS) and second harmonic generation (SHG) microscopy were used to detect and image osteogenic differentiation of human neural crest-derived inferior turbinate stem cells (ITSCs). Combined CARS and SHG microscopy was able to detect markers of osteogenesis within 14 days after osteogenic induction. This process increased during continued differentiation. Furthermore, Raman spectroscopy showed significant increases of the PO<jats:sub>4</jats:sub><jats:sup>3−</jats:sup> symmetric stretch vibrations at 959 cm<jats:sup>−1</jats:sup> assigned to calcium hydroxyapatite between days 14 and 21. Additionally, CARS microscopy was able to image calcium hydroxyapatite deposits within 14 days following osteogenic induction, which was confirmed by Alizarin Red-Staining and RT- PCR. Taken together, the multimodal label-free analysis methods Raman spectroscopy, CARS and SHG microscopy can monitor osteogenic differentiation of adult human stem cells into osteoblasts with high sensitivity and spatial resolution in three dimensions. Our findings suggest a great potential of these optical detection methods for clinical applications including <jats:italic>in vivo</jats:italic> observation of bone tissue–implant-interfaces or disease diagnosis.</jats:p> |
| container_issue |
1 |
| container_start_page |
0 |
| container_title |
Scientific Reports |
| container_volume |
6 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792344415153422341 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T17:07:11.422Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Label-free+nonlinear+optical+microscopy+detects+early+markers+for+osteogenic+differentiation+of+human+stem+cells&rft.date=2016-05-26&genre=article&issn=2045-2322&volume=6&issue=1&jtitle=Scientific+Reports&atitle=Label-free+nonlinear+optical+microscopy+detects+early+markers+for+osteogenic+differentiation+of+human+stem+cells&aulast=Kaltschmidt&aufirst=Barbara&rft_id=info%3Adoi%2F10.1038%2Fsrep26716&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792344415153422341 |
| author | Hofemeier, Arne D., Hachmeister, Henning, Pilger, Christian, Schürmann, Matthias, Greiner, Johannes F. W., Nolte, Lena, Sudhoff, Holger, Kaltschmidt, Christian, Huser, Thomas, Kaltschmidt, Barbara |
| author_facet | Hofemeier, Arne D., Hachmeister, Henning, Pilger, Christian, Schürmann, Matthias, Greiner, Johannes F. W., Nolte, Lena, Sudhoff, Holger, Kaltschmidt, Christian, Huser, Thomas, Kaltschmidt, Barbara, Hofemeier, Arne D., Hachmeister, Henning, Pilger, Christian, Schürmann, Matthias, Greiner, Johannes F. W., Nolte, Lena, Sudhoff, Holger, Kaltschmidt, Christian, Huser, Thomas, Kaltschmidt, Barbara |
| author_sort | hofemeier, arne d. |
| container_issue | 1 |
| container_start_page | 0 |
| container_title | Scientific Reports |
| container_volume | 6 |
| description | <jats:title>Abstract</jats:title><jats:p>Tissue engineering by stem cell differentiation is a novel treatment option for bone regeneration. Most approaches for the detection of osteogenic differentiation are invasive or destructive and not compatible with live cell analysis. Here, non-destructive and label-free approaches of Raman spectroscopy, coherent anti-Stokes Raman scattering (CARS) and second harmonic generation (SHG) microscopy were used to detect and image osteogenic differentiation of human neural crest-derived inferior turbinate stem cells (ITSCs). Combined CARS and SHG microscopy was able to detect markers of osteogenesis within 14 days after osteogenic induction. This process increased during continued differentiation. Furthermore, Raman spectroscopy showed significant increases of the PO<jats:sub>4</jats:sub><jats:sup>3−</jats:sup> symmetric stretch vibrations at 959 cm<jats:sup>−1</jats:sup> assigned to calcium hydroxyapatite between days 14 and 21. Additionally, CARS microscopy was able to image calcium hydroxyapatite deposits within 14 days following osteogenic induction, which was confirmed by Alizarin Red-Staining and RT- PCR. Taken together, the multimodal label-free analysis methods Raman spectroscopy, CARS and SHG microscopy can monitor osteogenic differentiation of adult human stem cells into osteoblasts with high sensitivity and spatial resolution in three dimensions. Our findings suggest a great potential of these optical detection methods for clinical applications including <jats:italic>in vivo</jats:italic> observation of bone tissue–implant-interfaces or disease diagnosis.</jats:p> |
| doi_str_mv | 10.1038/srep26716 |
| facet_avail | Online, Free |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTAzOC9zcmVwMjY3MTY |
| imprint | Springer Science and Business Media LLC, 2016 |
| imprint_str_mv | Springer Science and Business Media LLC, 2016 |
| institution | DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Zi4, DE-Gla1, DE-15, DE-Pl11, DE-Rs1, DE-14, DE-105, DE-Ch1, DE-L229, DE-D275 |
| issn | 2045-2322 |
| issn_str_mv | 2045-2322 |
| language | English |
| last_indexed | 2024-03-01T17:07:11.422Z |
| match_str | hofemeier2016labelfreenonlinearopticalmicroscopydetectsearlymarkersforosteogenicdifferentiationofhumanstemcells |
| mega_collection | Springer Science and Business Media LLC (CrossRef) |
| physical | |
| publishDate | 2016 |
| publishDateSort | 2016 |
| publisher | Springer Science and Business Media LLC |
| record_format | ai |
| recordtype | ai |
| series | Scientific Reports |
| source_id | 49 |
| spelling | Hofemeier, Arne D. Hachmeister, Henning Pilger, Christian Schürmann, Matthias Greiner, Johannes F. W. Nolte, Lena Sudhoff, Holger Kaltschmidt, Christian Huser, Thomas Kaltschmidt, Barbara 2045-2322 Springer Science and Business Media LLC Multidisciplinary http://dx.doi.org/10.1038/srep26716 <jats:title>Abstract</jats:title><jats:p>Tissue engineering by stem cell differentiation is a novel treatment option for bone regeneration. Most approaches for the detection of osteogenic differentiation are invasive or destructive and not compatible with live cell analysis. Here, non-destructive and label-free approaches of Raman spectroscopy, coherent anti-Stokes Raman scattering (CARS) and second harmonic generation (SHG) microscopy were used to detect and image osteogenic differentiation of human neural crest-derived inferior turbinate stem cells (ITSCs). Combined CARS and SHG microscopy was able to detect markers of osteogenesis within 14 days after osteogenic induction. This process increased during continued differentiation. Furthermore, Raman spectroscopy showed significant increases of the PO<jats:sub>4</jats:sub><jats:sup>3−</jats:sup> symmetric stretch vibrations at 959 cm<jats:sup>−1</jats:sup> assigned to calcium hydroxyapatite between days 14 and 21. Additionally, CARS microscopy was able to image calcium hydroxyapatite deposits within 14 days following osteogenic induction, which was confirmed by Alizarin Red-Staining and RT- PCR. Taken together, the multimodal label-free analysis methods Raman spectroscopy, CARS and SHG microscopy can monitor osteogenic differentiation of adult human stem cells into osteoblasts with high sensitivity and spatial resolution in three dimensions. Our findings suggest a great potential of these optical detection methods for clinical applications including <jats:italic>in vivo</jats:italic> observation of bone tissue–implant-interfaces or disease diagnosis.</jats:p> Label-free nonlinear optical microscopy detects early markers for osteogenic differentiation of human stem cells Scientific Reports |
| spellingShingle | Hofemeier, Arne D., Hachmeister, Henning, Pilger, Christian, Schürmann, Matthias, Greiner, Johannes F. W., Nolte, Lena, Sudhoff, Holger, Kaltschmidt, Christian, Huser, Thomas, Kaltschmidt, Barbara, Scientific Reports, Label-free nonlinear optical microscopy detects early markers for osteogenic differentiation of human stem cells, Multidisciplinary |
| title | Label-free nonlinear optical microscopy detects early markers for osteogenic differentiation of human stem cells |
| title_full | Label-free nonlinear optical microscopy detects early markers for osteogenic differentiation of human stem cells |
| title_fullStr | Label-free nonlinear optical microscopy detects early markers for osteogenic differentiation of human stem cells |
| title_full_unstemmed | Label-free nonlinear optical microscopy detects early markers for osteogenic differentiation of human stem cells |
| title_short | Label-free nonlinear optical microscopy detects early markers for osteogenic differentiation of human stem cells |
| title_sort | label-free nonlinear optical microscopy detects early markers for osteogenic differentiation of human stem cells |
| title_unstemmed | Label-free nonlinear optical microscopy detects early markers for osteogenic differentiation of human stem cells |
| topic | Multidisciplinary |
| url | http://dx.doi.org/10.1038/srep26716 |