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RELACS nuclei barcoding enables high-throughput ChIP-seq
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| Zeitschriftentitel: | Communications Biology |
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| Personen und Körperschaften: | , , , , , , , , , |
| In: | Communications Biology, 1, 2018, 1 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Springer Science and Business Media LLC
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| Schlagwörter: |
| author_facet |
Arrigoni, Laura Al-Hasani, Hoor Ramírez, Fidel Panzeri, Ilaria Ryan, Devon Patrick Santacruz, Diana Kress, Nadia Pospisilik, John Andrew Bönisch, Ulrike Manke, Thomas Arrigoni, Laura Al-Hasani, Hoor Ramírez, Fidel Panzeri, Ilaria Ryan, Devon Patrick Santacruz, Diana Kress, Nadia Pospisilik, John Andrew Bönisch, Ulrike Manke, Thomas |
|---|---|
| author |
Arrigoni, Laura Al-Hasani, Hoor Ramírez, Fidel Panzeri, Ilaria Ryan, Devon Patrick Santacruz, Diana Kress, Nadia Pospisilik, John Andrew Bönisch, Ulrike Manke, Thomas |
| spellingShingle |
Arrigoni, Laura Al-Hasani, Hoor Ramírez, Fidel Panzeri, Ilaria Ryan, Devon Patrick Santacruz, Diana Kress, Nadia Pospisilik, John Andrew Bönisch, Ulrike Manke, Thomas Communications Biology RELACS nuclei barcoding enables high-throughput ChIP-seq General Agricultural and Biological Sciences General Biochemistry, Genetics and Molecular Biology Medicine (miscellaneous) |
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arrigoni, laura |
| spelling |
Arrigoni, Laura Al-Hasani, Hoor Ramírez, Fidel Panzeri, Ilaria Ryan, Devon Patrick Santacruz, Diana Kress, Nadia Pospisilik, John Andrew Bönisch, Ulrike Manke, Thomas 2399-3642 Springer Science and Business Media LLC General Agricultural and Biological Sciences General Biochemistry, Genetics and Molecular Biology Medicine (miscellaneous) http://dx.doi.org/10.1038/s42003-018-0219-z <jats:title>Abstract</jats:title><jats:p>Chromatin immunoprecipitation followed by deep sequencing (ChIP-seq) is an invaluable tool for mapping chromatin-associated proteins. Current barcoding strategies aim to improve assay throughput and scalability but intense sample handling and lack of standardization over cell types, cell numbers and epitopes hinder wide-spread use in the field. Here, we present a barcoding method to enable high-throughput ChIP-seq using common molecular biology techniques. The method, called RELACS (restriction enzyme-based labeling of chromatin in situ) relies on standardized nuclei extraction from any source and employs chromatin cutting and barcoding within intact nuclei. Barcoded nuclei are pooled and processed within the same ChIP reaction, for maximal comparability and workload reduction. The innovative barcoding concept is particularly user-friendly and suitable for implementation to standardized large-scale clinical studies and scarce samples. Aiming to maximize universality and scalability, RELACS can generate ChIP-seq libraries for transcription factors and histone modifications from hundreds of samples within three days.</jats:p> RELACS nuclei barcoding enables high-throughput ChIP-seq Communications Biology |
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Springer Science and Business Media LLC |
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| title |
RELACS nuclei barcoding enables high-throughput ChIP-seq |
| title_unstemmed |
RELACS nuclei barcoding enables high-throughput ChIP-seq |
| title_full |
RELACS nuclei barcoding enables high-throughput ChIP-seq |
| title_fullStr |
RELACS nuclei barcoding enables high-throughput ChIP-seq |
| title_full_unstemmed |
RELACS nuclei barcoding enables high-throughput ChIP-seq |
| title_short |
RELACS nuclei barcoding enables high-throughput ChIP-seq |
| title_sort |
relacs nuclei barcoding enables high-throughput chip-seq |
| topic |
General Agricultural and Biological Sciences General Biochemistry, Genetics and Molecular Biology Medicine (miscellaneous) |
| url |
http://dx.doi.org/10.1038/s42003-018-0219-z |
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2018 |
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<jats:title>Abstract</jats:title><jats:p>Chromatin immunoprecipitation followed by deep sequencing (ChIP-seq) is an invaluable tool for mapping chromatin-associated proteins. Current barcoding strategies aim to improve assay throughput and scalability but intense sample handling and lack of standardization over cell types, cell numbers and epitopes hinder wide-spread use in the field. Here, we present a barcoding method to enable high-throughput ChIP-seq using common molecular biology techniques. The method, called RELACS (restriction enzyme-based labeling of chromatin in situ) relies on standardized nuclei extraction from any source and employs chromatin cutting and barcoding within intact nuclei. Barcoded nuclei are pooled and processed within the same ChIP reaction, for maximal comparability and workload reduction. The innovative barcoding concept is particularly user-friendly and suitable for implementation to standardized large-scale clinical studies and scarce samples. Aiming to maximize universality and scalability, RELACS can generate ChIP-seq libraries for transcription factors and histone modifications from hundreds of samples within three days.</jats:p> |
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| author | Arrigoni, Laura, Al-Hasani, Hoor, Ramírez, Fidel, Panzeri, Ilaria, Ryan, Devon Patrick, Santacruz, Diana, Kress, Nadia, Pospisilik, John Andrew, Bönisch, Ulrike, Manke, Thomas |
| author_facet | Arrigoni, Laura, Al-Hasani, Hoor, Ramírez, Fidel, Panzeri, Ilaria, Ryan, Devon Patrick, Santacruz, Diana, Kress, Nadia, Pospisilik, John Andrew, Bönisch, Ulrike, Manke, Thomas, Arrigoni, Laura, Al-Hasani, Hoor, Ramírez, Fidel, Panzeri, Ilaria, Ryan, Devon Patrick, Santacruz, Diana, Kress, Nadia, Pospisilik, John Andrew, Bönisch, Ulrike, Manke, Thomas |
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| description | <jats:title>Abstract</jats:title><jats:p>Chromatin immunoprecipitation followed by deep sequencing (ChIP-seq) is an invaluable tool for mapping chromatin-associated proteins. Current barcoding strategies aim to improve assay throughput and scalability but intense sample handling and lack of standardization over cell types, cell numbers and epitopes hinder wide-spread use in the field. Here, we present a barcoding method to enable high-throughput ChIP-seq using common molecular biology techniques. The method, called RELACS (restriction enzyme-based labeling of chromatin in situ) relies on standardized nuclei extraction from any source and employs chromatin cutting and barcoding within intact nuclei. Barcoded nuclei are pooled and processed within the same ChIP reaction, for maximal comparability and workload reduction. The innovative barcoding concept is particularly user-friendly and suitable for implementation to standardized large-scale clinical studies and scarce samples. Aiming to maximize universality and scalability, RELACS can generate ChIP-seq libraries for transcription factors and histone modifications from hundreds of samples within three days.</jats:p> |
| doi_str_mv | 10.1038/s42003-018-0219-z |
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| spelling | Arrigoni, Laura Al-Hasani, Hoor Ramírez, Fidel Panzeri, Ilaria Ryan, Devon Patrick Santacruz, Diana Kress, Nadia Pospisilik, John Andrew Bönisch, Ulrike Manke, Thomas 2399-3642 Springer Science and Business Media LLC General Agricultural and Biological Sciences General Biochemistry, Genetics and Molecular Biology Medicine (miscellaneous) http://dx.doi.org/10.1038/s42003-018-0219-z <jats:title>Abstract</jats:title><jats:p>Chromatin immunoprecipitation followed by deep sequencing (ChIP-seq) is an invaluable tool for mapping chromatin-associated proteins. Current barcoding strategies aim to improve assay throughput and scalability but intense sample handling and lack of standardization over cell types, cell numbers and epitopes hinder wide-spread use in the field. Here, we present a barcoding method to enable high-throughput ChIP-seq using common molecular biology techniques. The method, called RELACS (restriction enzyme-based labeling of chromatin in situ) relies on standardized nuclei extraction from any source and employs chromatin cutting and barcoding within intact nuclei. Barcoded nuclei are pooled and processed within the same ChIP reaction, for maximal comparability and workload reduction. The innovative barcoding concept is particularly user-friendly and suitable for implementation to standardized large-scale clinical studies and scarce samples. Aiming to maximize universality and scalability, RELACS can generate ChIP-seq libraries for transcription factors and histone modifications from hundreds of samples within three days.</jats:p> RELACS nuclei barcoding enables high-throughput ChIP-seq Communications Biology |
| spellingShingle | Arrigoni, Laura, Al-Hasani, Hoor, Ramírez, Fidel, Panzeri, Ilaria, Ryan, Devon Patrick, Santacruz, Diana, Kress, Nadia, Pospisilik, John Andrew, Bönisch, Ulrike, Manke, Thomas, Communications Biology, RELACS nuclei barcoding enables high-throughput ChIP-seq, General Agricultural and Biological Sciences, General Biochemistry, Genetics and Molecular Biology, Medicine (miscellaneous) |
| title | RELACS nuclei barcoding enables high-throughput ChIP-seq |
| title_full | RELACS nuclei barcoding enables high-throughput ChIP-seq |
| title_fullStr | RELACS nuclei barcoding enables high-throughput ChIP-seq |
| title_full_unstemmed | RELACS nuclei barcoding enables high-throughput ChIP-seq |
| title_short | RELACS nuclei barcoding enables high-throughput ChIP-seq |
| title_sort | relacs nuclei barcoding enables high-throughput chip-seq |
| title_unstemmed | RELACS nuclei barcoding enables high-throughput ChIP-seq |
| topic | General Agricultural and Biological Sciences, General Biochemistry, Genetics and Molecular Biology, Medicine (miscellaneous) |
| url | http://dx.doi.org/10.1038/s42003-018-0219-z |