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An oligonucleotide synthesizer based on a microreactor chip and an inkjet printer
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| Zeitschriftentitel: | Scientific Reports |
|---|---|
| Personen und Körperschaften: | , , , , , , |
| In: | Scientific Reports, 9, 2019, 1 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Springer Science and Business Media LLC
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| Schlagwörter: |
| author_facet |
Li, Hui Huang, Ye Wei, Zewen Wang, Wei Yang, Zhenjun Liang, Zicai Li, Zhihong Li, Hui Huang, Ye Wei, Zewen Wang, Wei Yang, Zhenjun Liang, Zicai Li, Zhihong |
|---|---|
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Li, Hui Huang, Ye Wei, Zewen Wang, Wei Yang, Zhenjun Liang, Zicai Li, Zhihong |
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Li, Hui Huang, Ye Wei, Zewen Wang, Wei Yang, Zhenjun Liang, Zicai Li, Zhihong Scientific Reports An oligonucleotide synthesizer based on a microreactor chip and an inkjet printer Multidisciplinary |
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li, hui |
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Li, Hui Huang, Ye Wei, Zewen Wang, Wei Yang, Zhenjun Liang, Zicai Li, Zhihong 2045-2322 Springer Science and Business Media LLC Multidisciplinary http://dx.doi.org/10.1038/s41598-019-41519-0 <jats:title>Abstract</jats:title><jats:p>Synthetic oligonucleotides (oligos) are important tools in the fields of molecular biology and genetic engineering. For applications requiring a large number of oligos with high concentration, it is critical to perform high throughput oligo synthesis and achieve high yield of each oligo. This study reports a microreactor chip for oligo synthesis. By incorporating silica beads in the microreactors, the surface area of the solid substrate for oligo synthesis increases significantly in each microreactor. These beads are fixed in the microreactors to withstand the flushing step in oligo synthesis. Compared to conventional synthesis methods, this design is able to avoid protocols to hold the beads and integrate more microreactors on a chip. An inkjet printer is utilized to deliver chemical reagents in the microreactors. To evaluate the feasibility of oligo synthesis using this proof-of-concept synthesizer, an oligo with six nucleotide units is successfully synthesized.</jats:p> An oligonucleotide synthesizer based on a microreactor chip and an inkjet printer Scientific Reports |
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An oligonucleotide synthesizer based on a microreactor chip and an inkjet printer |
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An oligonucleotide synthesizer based on a microreactor chip and an inkjet printer |
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An oligonucleotide synthesizer based on a microreactor chip and an inkjet printer |
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An oligonucleotide synthesizer based on a microreactor chip and an inkjet printer |
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An oligonucleotide synthesizer based on a microreactor chip and an inkjet printer |
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An oligonucleotide synthesizer based on a microreactor chip and an inkjet printer |
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an oligonucleotide synthesizer based on a microreactor chip and an inkjet printer |
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http://dx.doi.org/10.1038/s41598-019-41519-0 |
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<jats:title>Abstract</jats:title><jats:p>Synthetic oligonucleotides (oligos) are important tools in the fields of molecular biology and genetic engineering. For applications requiring a large number of oligos with high concentration, it is critical to perform high throughput oligo synthesis and achieve high yield of each oligo. This study reports a microreactor chip for oligo synthesis. By incorporating silica beads in the microreactors, the surface area of the solid substrate for oligo synthesis increases significantly in each microreactor. These beads are fixed in the microreactors to withstand the flushing step in oligo synthesis. Compared to conventional synthesis methods, this design is able to avoid protocols to hold the beads and integrate more microreactors on a chip. An inkjet printer is utilized to deliver chemical reagents in the microreactors. To evaluate the feasibility of oligo synthesis using this proof-of-concept synthesizer, an oligo with six nucleotide units is successfully synthesized.</jats:p> |
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| description | <jats:title>Abstract</jats:title><jats:p>Synthetic oligonucleotides (oligos) are important tools in the fields of molecular biology and genetic engineering. For applications requiring a large number of oligos with high concentration, it is critical to perform high throughput oligo synthesis and achieve high yield of each oligo. This study reports a microreactor chip for oligo synthesis. By incorporating silica beads in the microreactors, the surface area of the solid substrate for oligo synthesis increases significantly in each microreactor. These beads are fixed in the microreactors to withstand the flushing step in oligo synthesis. Compared to conventional synthesis methods, this design is able to avoid protocols to hold the beads and integrate more microreactors on a chip. An inkjet printer is utilized to deliver chemical reagents in the microreactors. To evaluate the feasibility of oligo synthesis using this proof-of-concept synthesizer, an oligo with six nucleotide units is successfully synthesized.</jats:p> |
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| spelling | Li, Hui Huang, Ye Wei, Zewen Wang, Wei Yang, Zhenjun Liang, Zicai Li, Zhihong 2045-2322 Springer Science and Business Media LLC Multidisciplinary http://dx.doi.org/10.1038/s41598-019-41519-0 <jats:title>Abstract</jats:title><jats:p>Synthetic oligonucleotides (oligos) are important tools in the fields of molecular biology and genetic engineering. For applications requiring a large number of oligos with high concentration, it is critical to perform high throughput oligo synthesis and achieve high yield of each oligo. This study reports a microreactor chip for oligo synthesis. By incorporating silica beads in the microreactors, the surface area of the solid substrate for oligo synthesis increases significantly in each microreactor. These beads are fixed in the microreactors to withstand the flushing step in oligo synthesis. Compared to conventional synthesis methods, this design is able to avoid protocols to hold the beads and integrate more microreactors on a chip. An inkjet printer is utilized to deliver chemical reagents in the microreactors. To evaluate the feasibility of oligo synthesis using this proof-of-concept synthesizer, an oligo with six nucleotide units is successfully synthesized.</jats:p> An oligonucleotide synthesizer based on a microreactor chip and an inkjet printer Scientific Reports |
| spellingShingle | Li, Hui, Huang, Ye, Wei, Zewen, Wang, Wei, Yang, Zhenjun, Liang, Zicai, Li, Zhihong, Scientific Reports, An oligonucleotide synthesizer based on a microreactor chip and an inkjet printer, Multidisciplinary |
| title | An oligonucleotide synthesizer based on a microreactor chip and an inkjet printer |
| title_full | An oligonucleotide synthesizer based on a microreactor chip and an inkjet printer |
| title_fullStr | An oligonucleotide synthesizer based on a microreactor chip and an inkjet printer |
| title_full_unstemmed | An oligonucleotide synthesizer based on a microreactor chip and an inkjet printer |
| title_short | An oligonucleotide synthesizer based on a microreactor chip and an inkjet printer |
| title_sort | an oligonucleotide synthesizer based on a microreactor chip and an inkjet printer |
| title_unstemmed | An oligonucleotide synthesizer based on a microreactor chip and an inkjet printer |
| topic | Multidisciplinary |
| url | http://dx.doi.org/10.1038/s41598-019-41519-0 |