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Metatranscriptomic exploration of microbial functioning in clouds

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Zeitschriftentitel: Scientific Reports
Personen und Körperschaften: Amato, Pierre, Besaury, Ludovic, Joly, Muriel, Penaud, Benjamin, Deguillaume, Laurent, Delort, Anne-Marie
In: Scientific Reports, 9, 2019, 1
Format: E-Article
Sprache: Englisch
veröffentlicht:
Springer Science and Business Media LLC
Schlagwörter:
Multidisciplinary
author_facet Amato, Pierre
Besaury, Ludovic
Joly, Muriel
Penaud, Benjamin
Deguillaume, Laurent
Delort, Anne-Marie
Amato, Pierre
Besaury, Ludovic
Joly, Muriel
Penaud, Benjamin
Deguillaume, Laurent
Delort, Anne-Marie
author Amato, Pierre
Besaury, Ludovic
Joly, Muriel
Penaud, Benjamin
Deguillaume, Laurent
Delort, Anne-Marie
spellingShingle Amato, Pierre
Besaury, Ludovic
Joly, Muriel
Penaud, Benjamin
Deguillaume, Laurent
Delort, Anne-Marie
Scientific Reports
Metatranscriptomic exploration of microbial functioning in clouds
Multidisciplinary
author_sort amato, pierre
spelling Amato, Pierre Besaury, Ludovic Joly, Muriel Penaud, Benjamin Deguillaume, Laurent Delort, Anne-Marie 2045-2322 Springer Science and Business Media LLC Multidisciplinary http://dx.doi.org/10.1038/s41598-019-41032-4 <jats:title>Abstract</jats:title><jats:p>Clouds constitute the uppermost layer of the biosphere. They host diverse communities whose functioning remains obscure, although biological activity potentially participates to atmospheric chemical and physical processes. In order to gain information on the metabolic functioning of microbial communities in clouds, we conducted coordinated metagenomics/metatranscriptomics profiling of cloud water microbial communities. Samples were collected from a high altitude atmospheric station in France and examined for biological content after untargeted amplification of nucleic acids. Living microorganisms, essentially bacteria, maintained transcriptional and translational activities and expressed many known complementary physiological responses intended to fight oxidants, osmotic variations and cold. These included activities of oxidant detoxification and regulation, synthesis of osmoprotectants/cryoprotectants, modifications of membranes, iron uptake. Consistently these energy-demanding processes were fueled by central metabolic routes involved in oxidative stress response and redox homeostasis management, such as pentose phosphate and glyoxylate pathways. Elevated binding and transmembrane ion transports demonstrated important interactions between cells and their cloud droplet chemical environments. In addition, polysaccharides, potentially beneficial for survival like exopolysaccharides, biosurfactants and adhesins, were synthesized. Our results support a biological influence on cloud physical and chemical processes, acting notably on the oxidant capacity, iron speciation and availability, amino-acids distribution and carbon and nitrogen fates.</jats:p> Metatranscriptomic exploration of microbial functioning in clouds Scientific Reports
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title Metatranscriptomic exploration of microbial functioning in clouds
title_unstemmed Metatranscriptomic exploration of microbial functioning in clouds
title_full Metatranscriptomic exploration of microbial functioning in clouds
title_fullStr Metatranscriptomic exploration of microbial functioning in clouds
title_full_unstemmed Metatranscriptomic exploration of microbial functioning in clouds
title_short Metatranscriptomic exploration of microbial functioning in clouds
title_sort metatranscriptomic exploration of microbial functioning in clouds
topic Multidisciplinary
url http://dx.doi.org/10.1038/s41598-019-41032-4
publishDate 2019
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description <jats:title>Abstract</jats:title><jats:p>Clouds constitute the uppermost layer of the biosphere. They host diverse communities whose functioning remains obscure, although biological activity potentially participates to atmospheric chemical and physical processes. In order to gain information on the metabolic functioning of microbial communities in clouds, we conducted coordinated metagenomics/metatranscriptomics profiling of cloud water microbial communities. Samples were collected from a high altitude atmospheric station in France and examined for biological content after untargeted amplification of nucleic acids. Living microorganisms, essentially bacteria, maintained transcriptional and translational activities and expressed many known complementary physiological responses intended to fight oxidants, osmotic variations and cold. These included activities of oxidant detoxification and regulation, synthesis of osmoprotectants/cryoprotectants, modifications of membranes, iron uptake. Consistently these energy-demanding processes were fueled by central metabolic routes involved in oxidative stress response and redox homeostasis management, such as pentose phosphate and glyoxylate pathways. Elevated binding and transmembrane ion transports demonstrated important interactions between cells and their cloud droplet chemical environments. In addition, polysaccharides, potentially beneficial for survival like exopolysaccharides, biosurfactants and adhesins, were synthesized. Our results support a biological influence on cloud physical and chemical processes, acting notably on the oxidant capacity, iron speciation and availability, amino-acids distribution and carbon and nitrogen fates.</jats:p>
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author Amato, Pierre, Besaury, Ludovic, Joly, Muriel, Penaud, Benjamin, Deguillaume, Laurent, Delort, Anne-Marie
author_facet Amato, Pierre, Besaury, Ludovic, Joly, Muriel, Penaud, Benjamin, Deguillaume, Laurent, Delort, Anne-Marie, Amato, Pierre, Besaury, Ludovic, Joly, Muriel, Penaud, Benjamin, Deguillaume, Laurent, Delort, Anne-Marie
author_sort amato, pierre
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description <jats:title>Abstract</jats:title><jats:p>Clouds constitute the uppermost layer of the biosphere. They host diverse communities whose functioning remains obscure, although biological activity potentially participates to atmospheric chemical and physical processes. In order to gain information on the metabolic functioning of microbial communities in clouds, we conducted coordinated metagenomics/metatranscriptomics profiling of cloud water microbial communities. Samples were collected from a high altitude atmospheric station in France and examined for biological content after untargeted amplification of nucleic acids. Living microorganisms, essentially bacteria, maintained transcriptional and translational activities and expressed many known complementary physiological responses intended to fight oxidants, osmotic variations and cold. These included activities of oxidant detoxification and regulation, synthesis of osmoprotectants/cryoprotectants, modifications of membranes, iron uptake. Consistently these energy-demanding processes were fueled by central metabolic routes involved in oxidative stress response and redox homeostasis management, such as pentose phosphate and glyoxylate pathways. Elevated binding and transmembrane ion transports demonstrated important interactions between cells and their cloud droplet chemical environments. In addition, polysaccharides, potentially beneficial for survival like exopolysaccharides, biosurfactants and adhesins, were synthesized. Our results support a biological influence on cloud physical and chemical processes, acting notably on the oxidant capacity, iron speciation and availability, amino-acids distribution and carbon and nitrogen fates.</jats:p>
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spelling Amato, Pierre Besaury, Ludovic Joly, Muriel Penaud, Benjamin Deguillaume, Laurent Delort, Anne-Marie 2045-2322 Springer Science and Business Media LLC Multidisciplinary http://dx.doi.org/10.1038/s41598-019-41032-4 <jats:title>Abstract</jats:title><jats:p>Clouds constitute the uppermost layer of the biosphere. They host diverse communities whose functioning remains obscure, although biological activity potentially participates to atmospheric chemical and physical processes. In order to gain information on the metabolic functioning of microbial communities in clouds, we conducted coordinated metagenomics/metatranscriptomics profiling of cloud water microbial communities. Samples were collected from a high altitude atmospheric station in France and examined for biological content after untargeted amplification of nucleic acids. Living microorganisms, essentially bacteria, maintained transcriptional and translational activities and expressed many known complementary physiological responses intended to fight oxidants, osmotic variations and cold. These included activities of oxidant detoxification and regulation, synthesis of osmoprotectants/cryoprotectants, modifications of membranes, iron uptake. Consistently these energy-demanding processes were fueled by central metabolic routes involved in oxidative stress response and redox homeostasis management, such as pentose phosphate and glyoxylate pathways. Elevated binding and transmembrane ion transports demonstrated important interactions between cells and their cloud droplet chemical environments. In addition, polysaccharides, potentially beneficial for survival like exopolysaccharides, biosurfactants and adhesins, were synthesized. Our results support a biological influence on cloud physical and chemical processes, acting notably on the oxidant capacity, iron speciation and availability, amino-acids distribution and carbon and nitrogen fates.</jats:p> Metatranscriptomic exploration of microbial functioning in clouds Scientific Reports
spellingShingle Amato, Pierre, Besaury, Ludovic, Joly, Muriel, Penaud, Benjamin, Deguillaume, Laurent, Delort, Anne-Marie, Scientific Reports, Metatranscriptomic exploration of microbial functioning in clouds, Multidisciplinary
title Metatranscriptomic exploration of microbial functioning in clouds
title_full Metatranscriptomic exploration of microbial functioning in clouds
title_fullStr Metatranscriptomic exploration of microbial functioning in clouds
title_full_unstemmed Metatranscriptomic exploration of microbial functioning in clouds
title_short Metatranscriptomic exploration of microbial functioning in clouds
title_sort metatranscriptomic exploration of microbial functioning in clouds
title_unstemmed Metatranscriptomic exploration of microbial functioning in clouds
topic Multidisciplinary
url http://dx.doi.org/10.1038/s41598-019-41032-4